Article
Immunology
Yi Chen, Jiamin Huang, Zaiwen Guo, Zhechen Zhu, Yiming Shao, Linbin Li, Yunxi Yang, Yanzhen Yu, Lu Liu, Bingwei Sun
Summary: In this study, the function and heterogeneity of neutrophils in neonatal umbilical cord blood (UCB) were analyzed using single-cell RNA sequencing analysis for the first time. The results showed that neutrophils in UCB were more naive and had more obvious degranulation and activation functions compared to healthy adults. Significant differences were also observed in the amount and function of a specific subset of neutrophils between UCB and healthy adults. Additionally, a new subset of neutrophils with distinct characteristics was identified. This study provides new insights into the mechanism of neutrophil-mediated diseases in neonates and the potential use of neutrophils in UCB.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Mailin Gan, Lin Liu, Shunhua Zhang, Zongyi Guo, Ya Tan, Jia Luo, Qiong Yang, Hongmei Pan, Xuewei Li, Jinyong Wang, Linyuan Shen, Li Zhu
Summary: Differential expression of porcine umbilical cord blood miRNA may have differences in immunity and angiogenesis, with great potential in the treatment of human diseases due to their ability for cross-species transmission. miRNA in cord blood may be beneficial for muscle-related diseases and liver- and brain-related diseases, providing insights for potential applications in mother-fetal communication.
Article
Agriculture, Dairy & Animal Science
Qiang Pu, Jie Chai, Li Chen, Changbao Liu, Changfeng Yang, Yongfu Huang, Jia Luo
Summary: The decline in reproductive potential of high-parity sows is mainly attributed to the repression of angiogenesis, thereby limiting the reproductive efficiency.
Review
Cell Biology
Yufeng Shang, Haotong Guan, Fuling Zhou
Summary: Umbilical cord mesenchymal stem cells (UC-MSCs) have the characteristics of inhibiting tumor cell proliferation and migration, supporting hematopoietic microenvironment, and suppressing the immune system, showing great potential in the treatment of hematologic diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Se-Hwa Jung, Ji-Eun You, Soon-Won Choi, Kyung-Sun Kang, Je-Yeol Cho, Jungmook Lyu, Pyung-Hwan Kim
Summary: The study investigated the biological function of the PKD1 gene in umbilical cord blood-derived mesenchymal stem cells (UCB-MSC), finding that it can enhance differentiation ability and maintain long-term stemness, providing new possibilities for stem cell therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Joanie Hare, Paulina Garcia DeLeon, Krystle Pool, Donna Reioux, Milford Fontenot, Richard E. Champlin, Erin N. Eaton, David Marin, Katayoun Rezvani, Elizabeth J. Shpall, Jeffrey M. Wilson, Chitra Hosing
Summary: The success of umbilical cord blood transplant depends on the dose of total nucleated and CD34+ cells infused, highlighting the importance of collecting high-quality cord blood units with high cell doses. Maternal gestational age and race, and neonatal weight and sex were found to correlate with the total nucleated cell dose, emphasizing the impact of these factors on cell dose. Optimized collection of umbilical cord blood is crucial for its use as a source of stem cells for transplantation.
Article
Cell & Tissue Engineering
Jessica M. Sun, Laura E. Case, Mohamad A. Mikati, Joan M. Jasien, Colleen McLaughlin, Barbara Waters-Pick, Gordon Worley, Jesse Troy, Joanne Kurtzberg
Summary: Studies suggest that appropriately dosed allogeneic sibling HLA-matched umbilical cord blood infusion has the potential to improve motor function in young children with cerebral palsy. This treatment is well tolerated and shows no adverse events related to the infusion.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Cell Biology
Anindita Roy, Guanlin Wang, Deena Iskander, Sorcha O'Byrne, Natalina Elliott, Jennifer O'Sullivan, Gemma Buck, Elisabeth F. Heuston, Wei Xiong Wen, Alba Rodriguez Meira, Peng Hua, Anastasios Karadimitris, Adam J. Mead, David M. Bodine, Irene Roberts, Bethan Psaila, Supat Thongjuea
Summary: Human hematopoiesis is a dynamic process that starts in utero 18-21 days post-conception, and understanding the site- and stage-specific variations is crucial for unraveling the origin of hematological disorders. The comparison of 57,489 HSPCs from 5 different tissues revealed significant transitions in cellular architecture and gene regulatory networks at different locations and developmental stages, with hematopoietic stem cells showing progression from cycling to quiescence during ontogeny. The dataset was also useful for understanding aberrant hematopoiesis in comparison to two cancers that present at distinct time points in postnatal life.
Article
Biochemistry & Molecular Biology
Silvia C. Rodrigues, Renato M. S. Cardoso, Patricia C. Freire, Claudia F. Gomes, Filipe V. Duarte, Ricardo Pires das Neves, Joana Simoes-Correia
Summary: The study demonstrates the immunomodulatory properties of small extracellular vesicles derived from umbilical cord blood mononuclear cells, which have the potential to be used for treating inflammatory skin diseases like psoriasis. These vesicles can shift macrophages to an anti-inflammatory phenotype, reduce T-cell proliferation and cytokine release, and support the development of regulatory T cells. Additionally, they show promising results in reducing psoriatic markers and acanthosis in a 3D psoriatic skin model, while increasing regulatory T cells in the skin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Jiasheng Wang, Leland Metheny
Summary: Cord blood is an important source for allogeneic hematopoietic cell transplantation and is also being developed for cell therapy products. The unique cellular composition of cord blood, including higher percentages of hematopoietic stem cells and progenitor cells, offers advantages in immune tolerance and off-the-shelf cell therapy development. In addition, cord blood-derived mesenchymal stem cells have potential in regenerative medicine and inflammatory/autoimmune conditions. This review focuses on recent clinical development of cord blood-derived therapies in oncology.
FRONTIERS IN ONCOLOGY
(2023)
Article
Cell Biology
Pei Tian, Andrew Elefanty, Edouard G. Stanley, Jennifer C. Durnall, Lachlan H. Thompson, Ngaire J. Elwood
Summary: Clinical-grade induced pluripotent stem cells (iPSCs) can be efficiently created using small volumes of thawed cord blood from public cord blood banks. This study presents a protocol that involves ex-vivo expansion and reprogramming of erythroid progenitor cells derived from cord blood, resulting in iPSC lines that are tested for pluripotency, genomic integrity, and stability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Environmental Sciences
Jiwon Oh, Hyeong-Moo Shin, Tomoko Nishimura, Mohammad Shafiur Rahman, Nagahide Takahashi, Kenji J. Tsuchiya
Summary: This study investigated the effects of prenatal exposure to PFAS on child cognitive development. The results showed mixed associations between PFOA and PFOS concentrations and cognitive functions at specific ages, but positive associations with longitudinal changes in cognitive development from 4 to 40 months of age.
ENVIRONMENT INTERNATIONAL
(2022)
Review
Immunology
Elena Boero, Giacomo Vezzani, Francesca Micoli, Mariagrazia Pizza, Omar Rossi
Summary: Shigella is a major global pathogen causing shigellosis, primarily affecting low- and middle-income countries. The role of antibodies in Shigella pathogenesis remains to be elucidated, especially since Shigella is mostly an intracellular pathogen. In vitro assays are important tools to evaluate the functionality of anti-Shigella antibodies, and animal models have been established to partially recapitulate human pathogenesis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Cell & Tissue Engineering
Gabriela Sanchez-Petitto, Katayoun Rezvani, May Daher, Hind Rafei, Partow Kebriaei, Elizabeth J. Shpall, Amanda Olson
Summary: Umbilical cord blood transplantation is an attractive alternative source of hematopoietic stem cells, with unique immune tolerance and engraftment kinetics. It has a lower risk of GvHD and comparable clinical outcomes to bone marrow transplantation. However, limitations in cell numbers and delayed engraftment pose challenges for its wider application. Research is being conducted to address these limitations and explore the potential of cord blood in treating various disorders.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Environmental Sciences
Anne P. Starling, Cheyret Wood, Cuining Liu, Katerina Kechris, Ivana V. Yang, Chloe Friedman, Deborah S. K. Thomas, Jennifer L. Peel, John L. Adgate, Sheryl Magzamen, Sheena E. Martenies, William B. Allshouse, Dana Dabelea
Summary: Prenatal exposure to ambient air pollution, such as PM2.5 and O3, can lead to changes in DNA methylation in umbilical cord blood cells, which are associated with various health outcomes in offspring. This study identified differentially methylated regions (DMRs) in cord blood cells associated with maternal exposure to PM2.5 and O3. The significant sites were annotated to genes involved in cardiometabolic disease, immune function and inflammation, and neurologic disorders. However, limited evidence was found for the mediation of the association between O3 exposure and infant adiposity by DNA methylation.
ENVIRONMENTAL RESEARCH
(2022)