Article
Veterinary Sciences
Jihoon Ryu, Gi-Jong Kang, Onnuri Kim, Jae-Yeon Park, Hyun-Jin Shin
Summary: This study found possible vertical transmission of PEDV through the placenta, which is crucial for controlling the disease.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Tong Ding, Ting Cheng, Xuerui Zhu, Wenwen Xiao, Sijin Xia, Liurong Fang, Puxian Fang, Shaobo Xiao
Summary: Porcine epidemic diarrhea virus (PEDV) is a highly pathogenic enteric coronavirus that causes severe enteritis and lethal watery diarrhea in suckling piglets. In this study, it was found that exosomes from PEDV-infected cells contain viral genomic RNA and viral nucleocapsid protein, and these exosomes can transmit the virus to both susceptible and non-susceptible cells. This study reveals a potential immune evasion mechanism utilized by PEDV and provides new insight into the transmission and infection of this important pathogen.
VETERINARY MICROBIOLOGY
(2023)
Article
Nutrition & Dietetics
Cristina Rosell-Cardona, Concepcio Amat, Christian Grinan-Ferre, Javier Polo, Merce Pallas, Anna Perez-Bosque, Miquel Moreto, Lluisa Miro
Summary: Supplementation with spray-dried porcine plasma (SDP) reduces Alzheimer's disease (AD) hallmarks in aging mice, potentially through the gut microbiota-gut-brain axis. SDP promotes the proliferation of probiotic species while reducing inflammation-associated bacteria, resulting in anti-inflammatory effects and neuroprotection.
Article
Microbiology
Zhiwei Li, Zhiqian Ma, Linfang Dong, Ting Yang, Yang Li, Dian Jiao, Weiguo Han, Haixue Zheng, Shuqi Xiao
Summary: This study confirms that the S gene is the decisive gene for PEDV adaptability to monkey Vero cells and porcine LLC-PK1 cells. The exchange of S1 and S2 subunits in adaptive strains does not enable PEDV to adapt to cells, and the 803L and 976H residues in the S2 subunit are critical for rCH/SX/2016-S1(HNXP)+S3(HNXP) adaptation to Vero cells. These findings highlight the crucial role of the PEDV S protein in cell tropism and suggest the potential role of coronaviruses S protein in cross-species transmission. Additionally, this study provides unique insights into identifying PEDV functional receptors and developing PEDV and other coronaviruses vaccines.
Article
Pharmacology & Pharmacy
Hongyu Rao, Weiwei Su, Xiaoli Zhang, Yonggang Wang, Tao Li, Jinliang Li, Xuan Zeng, Peibo Li
Summary: In this study, Hypericum japonicum extract (HJ) was found to have antiviral effects against PEDV. In vitro experiments showed that HJ could directly inactivate PEDV and inhibit its proliferation at non-cytotoxic concentrations. In vivo experiments demonstrated that HJ could reduce viral titers in the intestines of infected piglets and improve their intestinal pathological, suggesting that HJ has the potential to be developed as an anti-PEDV drug.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Haifei Wang, Peng Hui, Yoshinobu Uemoto, Yueyun Ding, Zongjun Yin, Wenbin Bao
Summary: This study investigated the metabolic and proteomic characteristics of PEDV-infected porcine intestinal epithelial cells using liquid chromatography tandem mass spectrometry and isobaric tags for relative and absolute quantification techniques. Changes in metabolism related to cysteine and methionine metabolism, glycine, serine, and threonine metabolism, and mineral absorption were observed. The betaine-homocysteine S-methyltransferase (BHMT) gene was found to potentially regulate these processes and knockdown of BHMT decreased PEDV replication and virus titers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Yiling Zhang, Shujuan Zhang, Zhiwei Sun, Xiangyang Liu, Guisong Liao, Zheng Niu, Zifei Kan, Shasha Xu, Jingyi Zhang, Hong Zou, Xingcui Zhang, Zhenhui Song
Summary: Porcine Epidemic Diarrhea Virus (PEDV) is a significant cause of diarrhea and death in piglets. This study found that PEDV induces diarrhea in piglets by inhibiting Na+ absorption in pig intestinal epithelial cells. The study also demonstrated the importance of EGFR in regulating NHE3 activity after PEDV infection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Hongliang Zhang, Yingguang Li, Ruimei Yang, Ling Xiao, Shaoming Dong, Jiaxu Lin, Gang Liu, Hu Shan
Summary: In this study, it was found that erastin can significantly inhibit the replication of PEDV virus in cells, and it functions by regulating the gene expression of the ferroptosis pathway. This suggests that erastin may be a potential drug for the treatment of PEDV infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Kazimierska, Wioletta Biel
Summary: Spray-dried porcine plasma (SDPP) and hydrolyzed porcine protein (HPP) are promising animal protein ingredients with high protein and mineral content. SDPP outperforms HPP in terms of protein content and molybdenum levels, making it an excellent source of protein.
Article
Virology
Mingxia Li, Liping Zhang, Li Pan, Peng Zhou, Ruiming Yu, Zhongwang Zhang, Jianliang Lv, Huichen Guo, Yonglu Wang, Sa Xiao, Xinsheng Liu
Summary: In this study, the researchers found that nicotinamide (NAM) effectively inhibits the replication of PEDV and PDCoV by primarily targeting their RNA and protein synthesis processes. The activation of the ERK1/2/MAPK pathway and downregulation of transcription factors also contribute to the inhibition of virus replication. This suggests that NAM could be a promising antiviral candidate not only for swine intestinal coronaviruses but also for other human and animal coronaviruses.
Article
Microbiology
Yingshuo Sun, Ting Gong, Dongdong Wu, Yongzhi Feng, Qi Gao, Jiabao Xing, Xiaoyu Zheng, Zebu Song, Xing Liu, Xiongnan Chen, Yankuo Sun, Guihong Zhang, Lang Gong
Summary: In this study, two strains of PEDV were isolated from diarrheal samples collected from two new PED outbreak farms in 2022. The strains were analyzed for their gene sequences and pathogenicity. The results showed that both strains belong to the G2c subtype and can cause severe diarrhea and intestinal tissue lesions in infected piglets. It was also found that PEDV can be detected in various organs, with the highest levels in the intestinal tissues.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Mingxiang Li, Yiye Zhang, Yuxin Fang, Shaobo Xiao, Puxian Fang, Liurong Fang
Summary: Porcine epidemic diarrhea virus (PEDV) is a re-emerging enteropathogenic coronavirus. In this study, a recombinant trypsin-independent PEDV strain was developed, which can provide protection against both G1 and G2 PEDV infections and does not require trypsin for propagation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Jie Ren, Zhen Ding, Puxian Fang, Shaobo Xiao, Liurong Fang
Summary: PEDV nsp13 is a crucial protein with ATPase and helicase activities that play a key role in unwinding viral RNA. By studying its biochemical properties, new insights have been provided for the development of antiviral drugs.
VETERINARY MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Baicheng Huang, Guoqian Gu, Yunjing Zhang, Zhenzhen Chen, Kegong Tian
Summary: This study analyzed the complete genomes of 647 strains of the highly contagious porcine epidemic diarrhea virus (PEDV) to understand its evolutionary characteristics. The results showed that the S protein, especially its S1 domain, experienced high positive selective pressure. The S gene was found to be more representative of the evolutionary relationship at the genome-wide level, and the haplotype networks revealed geographical clustering of strains. These findings provide valuable insights into the evolution of PEDV and offer guidance for developing effective prevention measures.
Article
Virology
Montserrat-Elemi Garcia-Hernandez, Maria-Elena Trujillo-Ortega, Sofia-Lizbeth Alcaraz-Estrada, Luis Lozano-Aguirre-Beltran, Carlos Sandoval-Jaime, Blanca Itzel Taboada-Ramirez, Rosa-Elena Sarmiento-Silva
Summary: This study revealed a high rate of coinfection of PEDV and PKV in piglets with acute diarrhea, providing experimental evidence for the importance of interactions between these pathogens and showcasing the NGS approach for gaining a better perspective on circulating viruses and pathogens in affected production units.
Article
Immunology
Ameneh Khatami, Rebecca Burrell, Brendan J. McMullan, William Rawlinson, Rodney C. Givney, Jen Kok, Soren Alexandersen, Cheryl A. Jones, Kristine K. Macartney, Philip N. Britton
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2020)
Article
Virology
Tarka Raj Bhatta, Anthony Chamings, Jessy Vibin, Marcel Klaassen, Soren Alexandersen
Article
Multidisciplinary Sciences
Jessy Vibin, Anthony Chamings, Marcel Klaassen, Tarka Raj Bhatta, Soren Alexandersen
SCIENTIFIC REPORTS
(2020)
Article
Virology
Tarka Raj Bhatta, Pia Ryt-Hansen, Jens Peter Nielsen, Lars Erik Larsen, Inge Larsen, Anthony Chamings, Nicole B. Goecke, Soren Alexandersen
Article
Virology
Tarka Raj Bhatta, Anthony Chamings, Soren Alexandersen
Summary: This study used a metagenomics approach to identify and characterize potential pathogens in colon and lung samples from pigs with diarrhea and poor growth in an Australian pig herd, revealing a wide diversity of porcine viruses including RNA and single-stranded DNA viruses, as well as the presence of bacteria such as Lawsonia intracellularis, Brachyspira spp., and Campylobacter spp., suggesting a complex interaction among microorganisms contributing to clinical disease and poor growth in pigs.
Article
Microbiology
Vanina Guernier-Cambert, Anthony Chamings, Fiona Collier, Soren Alexandersen
Summary: The study found that antimicrobial resistance genes are widely present and diverse in the gut microbiota of clinically healthy individuals from south-eastern Victoria, Australia. These genes mainly belong to classes of antibiotics such as tetracycline, beta-lactams, and MLSB, with the most commonly identified bacterial Orders carrying them being Clostridiales, Bacteroidales, and Enterobacteriales.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Pediatrics
Adelene Hilbig, Kwee C. Liew, Claire Foster, David G. Fuller, Anthony Chamings, Soren Alexandersen
JOURNAL OF PAEDIATRICS AND CHILD HEALTH
(2022)
Article
Multidisciplinary Sciences
Berta Blanch-Lazaro, Raoul F. H. Ribot, Mathew L. Berg, Soren Alexandersen, Andrew T. D. Bennett
Summary: This study found that detection of antibodies decreases over time when stored at room temperature on filter paper, leading to a higher risk of false negative results. Serum should be the preferred sample type for seroepidemiological studies on BFDV. Crimson rosellas have the potential to resist BFDV infection, especially adults which can produce high levels of antibodies.
Article
Immunology
Seweryn Bialasiewicz, Meryta May, Sarah Tozer, Rebecca Day, Anne Bernard, Julian Zaugg, Kyana Gartrell, Soren Alexandersen, Anthony Chamings, Claire Y. T. Wang, Julia Clark, Keith Grimwood, Claire Heney, Luregn Schlapbach, Robert S. Ware, David Speers, Ross M. Andrews, Stephen Lambert
Summary: A study found that the recombinant strain of human parechovirus 3 (HPeV3-AR) was the dominant strain in the outbreaks of sepsis-like illnesses in infants in Australia. However, this strain was not associated with increased disease severity.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Editorial Material
Veterinary Sciences
Grant Maxie
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Editorial Material
Veterinary Sciences
Grant Maxie
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Editorial Material
Veterinary Sciences
Grant Maxie
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Editorial Material
Veterinary Sciences
Grant Maxie
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Medicine, General & Internal
Man-Hung Eric Tang, Kim Lee Ng, Sofie Marie Edslev, Kirsten Ellegaard, Marc Stegger, Soren Alexandersen, Danish COVID Genom Consortium DCGC
Summary: A study found no significant statistical relationship between the levels of subgenomic RNAs in SARS-CoV-2 and host-related factors, suggesting that differences in target cell tropism, viral replication/transcription kinetics, or gene sequence features may contribute to the observed differences between lineages.
Letter
Immunology
Klara M. Andersen, Lasse S. Vestergaard, Jakob N. Nissen, Sophie J. George, Pia Ryt-Hansen, Charlotte K. Hjulsager, Jesper S. Krog, Marianne N. Skov, Soren Alexandersen, Lars E. Larsen, Ramona Trebbien
Summary: A zoonotic swine influenza A virus was detected in a severely ill patient during routine surveillance at the National Influenza Center, Denmark. We provide clinical and genetic characterization of this variant virus, which differs from another variant previously found in Denmark.
EMERGING INFECTIOUS DISEASES
(2022)