Article
Biochemistry & Molecular Biology
Kemal Ugur Tufekci, Begum Alural, Emre Tarakcioglu, Tugba San, Sermin Genc
Summary: In this study, it was found that lithium can protect neuronal cells from senescence through the miR-34a/Sirt1/p53 pathway. This suggests a potential novel treatment strategy for neurodegenerative diseases involving lithium in preventing neuronal senescence at the molecular level.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Oncology
Andrew Chin, Javier Mariscal, Minhyung Kim, Giorgia Guerra, Blandine Victor, Chen Qian, Elisabetta Broseghini, Edwin Posadas, Michael R. Freeman, Shivani Sharma, Paolo Gandellini, Nadia Zaffaroni, Sungyong You, Keith Syson Chan, Jlenia Guarnerio, Muller Fabbri, Dolores Di Vizio
Summary: This study reveals a novel mechanism of extracellular vesicles (EVs) shedding, in which the inhibition of SEC23A by miR-1227 induces a shift in EV shedding, favoring the shedding of large EV over small EV.
Article
Medicine, Research & Experimental
Qiuxia Li, Quanjiang Zhang
Summary: MicroRNAs (miRNAs) are small endogenous RNAs that regulate gene expression by binding to the mRNAs of protein-coding genes. In endothelial cells, miR-34a is an early identified miRNA that plays important roles in regulating endothelial functions, angiogenesis, differentiation, senescence, inflammatory response, responses to shear stress, and mitochondrial function. This review provides an overview of the current understanding of miR-34a in endothelial biology and discusses its potential as a therapeutic target for vascular diseases.
Article
Medicine, Research & Experimental
Zongwei Li, Yaying Song, Tingting He, Ruoxue Wen, Yongfang Li, Tingting Chen, Shuxian Huang, Yongting Wang, Yaohui Tang, FanXia Shen, Heng-Li Tian, Guo-Yuan Yang, Zhijun Zhang
Summary: M2 microglial small extracellular vesicles decreased glial scar formation and promoted recovery after stroke through miR-124, inhibiting astrocyte proliferation and transforming them into neuronal progenitor cells. These vesicles also downregulated Notch 1 expression and upregulated Sox2 expression in astrocytes.
Article
Biochemistry & Molecular Biology
Jiunn-Miin Lin, Chih-Hsiang Hsu, Jeen-Chen Chen, Shao-Hsuan Kao, You-Cian Lin
Summary: The study revealed that BCL-6 increases H3K27me3 levels in the promoter region of miR-34a by recruiting EZH2, leading to the inhibition of miR-34a expression and subsequent suppression of cardiomyocyte apoptosis, thus providing cardiac protection in AMI models.
Article
Chemistry, Medicinal
Li-tao Yi, Ji-xiao Zhu, Shu-qi Dong, Min Chen, Cheng-fu Li
Summary: The study demonstrated the antidepressant-like effects of berberine in mice exposed to chronic stress, primarily achieved by regulating the expression level of miR-34a. Berberine reversed behavioral changes and impaired neurogenesis induced by chronic stress, which was blocked by the intervention of miR-34a.
CHINESE HERBAL MEDICINES
(2021)
Article
Chemistry, Multidisciplinary
Esra Dirimtekin, Maria Mortoglou, Ceren Alavanda, Asmaa Benomar Yemlahi, Esra Arslan Ates, Ilter Guney, Pinar Uysal-Onganer
Summary: miR-34a is downregulated and associated with inhibition of tumor growth and invasion in ovarian cancer, while FOXP1 is reported as either an oncogene or tumor suppressor. It is found that miR-34a and FOXP1 are reversely correlated, and inhibition of miR-34a leads to upregulation of FOXP1 expression and increased cellular invasion. miR-34a could be a potential biomarker for improving the diagnostic efficiency of ovarian cancer, and its overexpression may reduce pathogenesis by targeting FOXP1.
Article
Cell Biology
Andy N. Vo, Srikanya Kundu, Caroline Strong, Olive Jung, Emily Lee, Min Jae Song, Molly E. Boutin, Michael Raghunath, Marc Ferrer
Summary: The neuroglial extracellular matrix (ECM) provides crucial physiological cues for neuronal cells in the brain. However, most in vitro studies lack these cues as cells are grown on stiff surfaces. Macromolecular crowding (MMC) can induce the deposition of native ECM by cells in culture. Using MMC, it was found that human astrocytes cultured with Ficoll and vitamin C showed enhanced deposition of brain ECM. Neural cultures grown on this astrocyte-ECM bed exhibited improved growth and functionality.
Article
Cell Biology
Yazhen Xu, Bingyu Guo, Xiaoyan Liu, Kai Tao
Summary: miR-34a inhibits melanoma growth by targeting ZEB1, suggesting a potential novel target for melanoma treatment.
Article
Multidisciplinary Sciences
Nadia Z. Shaban, Nashwa K. Ibrahim, Helen N. Saada, Fatma H. El-Rashidy, Heba M. Shaaban, Mostafa A. Farrag, Khalid Debaiky, Ahmad S. Kodous
Summary: The expression of miR-21 is upregulated in female breast cancer patients, while the expression of miR-34a is downregulated. Chemo-radiotherapy treatment decreases miR-21 expression and increases miR-34a expression. MiR-21 is directly correlated with Bcl2, while miR-34a is directly correlated with BRCA1, BRCA2, and p53. MiR-34a and miR-21 may be promising non-invasive biomarkers for evaluating the response of female breast cancer patients to chemo-radiotherapy.
JOURNAL OF RADIATION RESEARCH AND APPLIED SCIENCES
(2022)
Editorial Material
Oncology
Sophie Mockly, Herve Seitz
Summary: In this article titled "Analysis of the failure of miR-34a clinical trial", the authors review the reasons behind the disappointing outcomes of a clinical trial involving miR-34a, a potential tumor suppressor gene, and discuss the implications for other miRNA studies.
BRITISH JOURNAL OF CANCER
(2023)
Article
Biotechnology & Applied Microbiology
Yingying Wang, Baiyan Wang, Yangfan Xiao, Qingchun Cai, Junyue Xing, Hao Tang, Ruiqin Li, Hongtao Zhang
Summary: This study provides a novel approach for gene therapy based on miR-34a by constructing a charge-regulated, hydrophobic-modified, and functionally modified polyethylenimine (PEI) gene delivery vector. The vector shows efficient compaction of miRNAs into positively charged nanoparticles and possesses perfect degradability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Pawel Krzyzek, Beatrice Marinacci, Irene Vitale, Rossella Grande
Summary: The proper functioning of the human body has been extensively studied, and probiotics have been found to have a significant impact on human health through their production of extracellular vesicles. These vesicles contribute to maintaining the balance of microorganisms, regulating the immune system, enhancing digestion, and preventing or alleviating diseases. However, the health-promoting effects of extracellular vesicles secreted by non-pathogenic microorganisms, including probiotics, are not well understood. This review article aims to provide the latest literature data on the health-inducing properties of probiotics' extracellular vesicles, including their physicochemical properties and proteome.
Review
Chemistry, Multidisciplinary
Fei Teng, Martin Fussenegger
Summary: Extracellular vesicles (EVs) are nano-sized secreted vesicles containing various biomolecules, which have low immunogenicity and can functionally modify recipient cells by transferring bioactive constituents. Therefore, they are considered as an excellent candidate for the next generation drug delivery system.
Article
Neurosciences
Yi-Jun Li, Qing-Wen Xu, Cong-Hui Xu, Wei-Ming Li
Summary: In this study, it was found that mesenchymal stem cells (MSC) improved intestinal ischemia/reperfusion (I/R) injury by secreting exosomal miR-34a-5p and through METTL3/IGF2BP3-mediated pre-miR-34A m(6)A modification.
MOLECULAR NEUROBIOLOGY
(2022)