Article
Biochemistry & Molecular Biology
Md Abu Sayem Khan, Md Ibrahim Miah, Sabita Rezwana Rahman
Summary: This study utilized integrated reverse vaccinology and immunoinformatics approach to construct multi-epitope vaccines targeting essential proteins of Cryptococcus neoformans. The vaccines showed high antigenicity, stability, and generated robust immune responses.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Microbiology
Charles A. Specht, E. Jane Homan, Chrono K. Lee, Zhongming Mou, Christina L. Gomez, Maureen M. Hester, Ambily Abraham, Florentina Rus, Gary R. Ostroff, Stuart M. Levitz
Summary: This study reveals the potential of protein peptides in the development of cryptococcosis vaccines, and demonstrates that peptide vaccines can effectively protect mice from cryptococcosis.
Article
Microbiology
Yina Wang, Keyi Wang, Amariliz Rivera, Chaoyang Xue
Summary: Invasive fungal infections, such as cryptococcosis, are difficult to treat and lack effective vaccines. However, heat-killed fbp1 Delta cells (HK-fbp1) have shown potential as a vaccine candidate, providing immunity against lethal fungal pathogens. This study revealed that T cells, specifically CD4(+) or CD8(+) T cells, are critical for the vaccine-induced protection. Additionally, HK-fbp1 administration can inhibit disease dissemination when used as a therapeutic agent at the early stage of infection.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Tyler G. Normile, Maurizio Del Poeta
Summary: This study found that the Cryptococcus neoformans Delta sgl1 mutant has the potential to be a vaccine for preventing and treating cryptococcosis, providing effective protection even in immunocompromised conditions. It was also discovered that the HK Delta sgl1 vaccine can prevent the reactivation of lung granuloma after immunosuppression.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Enoch Tay, Sharon C-A Chen, Wendy Green, Ronald Lopez, Catriona L. Halliday
Summary: This study developed a new real-time PCR assay that can diagnose cryptococcosis rapidly and accurately, and differentiate between the two major causative agents. The assay showed high sensitivity and specificity, and can be applied to diverse clinical specimens.
Article
Microbiology
Yang Zhang, Liyan Zhou, Yan Liu, Xi Zhao, Xianqiang Lian, Jie Zhang, De Zhao, Yujuan Wang, Jin Zhong, Junfeng Wang, Hongli Wang, Linqi Wang, Yu Fu
Summary: SP1, a peptide derived from yeast GAPDH, has the potential to be developed as a treatment option for cryptococcosis by efficiently killing Cryptococcus neoformans and Cryptococcus gattii.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Pin Liang, Jiquan Song, Qin Liu
Summary: This study found that the combination of everolimus with two antifungal drugs, amphotericin B and azoles, showed synergistic effects in inhibiting Cryptococcus. The combination therapy also significantly lowered the minimum inhibitory concentrations of the antifungal drugs. These findings suggest that this combination treatment may be a potential therapeutic strategy for Cryptococcus infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
John W. Baddley, Sharon C-A Chen, Carrie Huisingh, Kaitlin Benedict, Emilio E. DeBess, Eleni Galanis, Brendan R. Jackson, Laura MacDougall, Nicola Marsden-Haug, Hanna Oltean, John R. Perfect, Peter Phillips, Tania C. Sorrell, Peter G. Pappas
Summary: This study compared patients with cryptococcosis caused by Cryptococcus neoformans and Cryptococcus gattii, revealing differences in characteristics, clinical manifestations, and treatment outcomes between the two species.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Chemistry, Medicinal
R. Madison Green, Kevin L. Bicker
Summary: RMG8-8 is a novel antifungal peptoid that shows limited efficacy against Candida albicans but excellent efficacy against Cryptococcus neoformans. This compound exhibits minimal cytotoxicity and good selectivity. Further studies are needed to explore its pharmacological importance and killing kinetics, as well as its plasma protein binding and proteolytic stability.
ACS MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Microbiology
Jianfeng Lin, Tuyetnhu Pham, Kenton Hipsher, Nathan Glueck, Yumeng Fan, Xiaorong Lin
Summary: The overexpression of Znf2 in Cryptococcus plays a crucial role in the interaction with the host, leading to protective immune responses. Components enriched in ZNF2(oe) cells are found to be immuno-protective, with antigens located within the capsule being essential for host protection.
Article
Microbiology
Jianfeng Lin, Tuyetnhu Pham, Kenton Hipsher, Nathan Glueck, Yumeng Fan, Xiaorong Lin
Summary: Systemic cryptococcosis is a fatal disease without treatment, causing 180,000 deaths annually worldwide. Vaccination with cryptococcal cells overexpressing ZNF2 provides significant protection to the host from subsequent challenge by the wild-type strain and may have clinical applications. Identifying immunoprotective components enriched in ZNF2(oe) cells and understanding the role of the capsule in host protection are crucial for developing effective vaccines.
Article
Biochemistry & Molecular Biology
Santhasiri Orrapin, Sittiruk Roytrakul, Narumon Phaonakrop, Siriwan Thaisakun, Khajornsak Tragoolpua, Amornrat Intorasoot, Suzanne McGill, Richard Burchmore, Sorasak Intorasoot
Summary: The study evaluated the biological activities of recombinant javanicin against the human fungal pathogen Cryptococcus neoformans, showing concentration- and time-dependent antifungal activity. Microscopic and proteomics analysis indicated that javanicin may inhibit fungal growth by interfering with carbohydrate metabolism and energy production pathways.
Article
Biotechnology & Applied Microbiology
Weidong Qian, Wenjing Wang, Jianing Zhang, Yuting Fu, Qiming Liu, Xinchen Li, Ting Wang, Qian Zhang
Summary: The research evaluated the antifungal and antibiofilm activities of plumbagin against Cryptococcus neoformans and identified its underlying mechanisms. Plumbagin disrupted cell membrane integrity and reduced metabolic activities of C. neoformans. Plumbagin damaged biofilm cells and dispersed mature biofilms by regulating gene expression involved in important cellular processes. This study suggests that plumbagin can be utilized as an antifungal agent against C. neoformans infections.
Article
Immunology
Weidong Qian, Xinchen Li, Qiming Liu, Jiaxing Lu, Ting Wang, Qian Zhang
Summary: Paeonol exhibits antifungal and antibiofilm activities against Candida albicans and Cryptococcus neoformans, disrupting cell membrane integrity, inhibiting biofilm formation, and affecting yeast-to-hyphal transition.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Magdalena Florek, Urszula Nawrot, Agnieszka Korzeniowska-Kowal, Katarzyna Wlodarczyk, Anna Wzorek, Anna Wozniak-Biel, Magdalena Brzozowska, Jozef Galli, Anna Bogucka, Jaroslaw Krol
Summary: Fungi of Cryptococcus neoformans/C. gattii species complex are pathogens causing severe infections in humans and animals, with a mortality rate of up to 70% in humans. Understanding the population structure and distribution of these pathogens is crucial for studying the epidemiology of cryptococcosis. A study in Poland revealed a population of C. neoformans strains in animals, some of which exhibited resistance to antifungal treatment.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Chrissy M. Leopold Wager, Camaron R. Hole, Karen L. Wozniak, Michal A. Olszewski, Mathias Mueller, Floyd L. Wormley
INFECTION AND IMMUNITY
(2015)
Article
Immunology
Camaron R. Hole, Chrissy M. Leopold Wager, Andrew S. Mendiola, Karen L. Wozniak, Althea Campuzano, Xin Lin, Floyd L. Wormley
INFECTION AND IMMUNITY
(2016)
Review
Microbiology
Chrissy M. Leopold Wager, Camaron R. Hole, Karen L. Woznia, Floyd L. Wormley
FRONTIERS IN MICROBIOLOGY
(2016)
Article
Microbiology
Kyla S. Ost, Shannon K. Esher, Chrissy M. Leopold Wager, Louise Walker, Jeanette Wagener, Carol Munro, Floyd L. Wormley, J. Andrew Alspaugh
Article
Immunology
Marley C. Caballero Van Dyke, Ashok K. Chaturvedi, Sarah E. Hardison, Chrissy M. Leopold Wager, Natalia Castro-Lopez, Camaron R. Hole, Karen L. Wozniak, Floyd L. Wormley
FRONTIERS IN IMMUNOLOGY
(2017)
Article
Immunology
Chrissy M. Leopold Wager, Camaron R. Hole, Karen L. Wozniak, Michal A. Olszewski, Floyd L. Wormley
JOURNAL OF IMMUNOLOGY
(2014)
Review
Immunology
C. M. Leopold Wager, F. L. Wormley
MUCOSAL IMMUNOLOGY
(2014)
Article
Multidisciplinary Sciences
Ashok K. Chaturvedi, Rumanasma S. Hameed, Karen L. Wozniak, Camaron R. Hole, Chrissy M. Leopold Wager, Susan T. Weintraub, Jose L. Lopez-Ribot, Floyd L. Wormley
Article
Multidisciplinary Sciences
Althea Campuzano, Natalia Castro-Lopez, Karen L. Wozniak, Chrissy M. Leopold Wager, Floyd L. Wormley
Article
Microbiology
Chrissy M. Leopold Wager, Camaron R. Hole, Althea Campuzano, Natalia Castro-Lopez, Hong Cai, Marley C. Caballero Van Dyke, Karen L. Wozniak, Yufeng Wang, Floyd L. Wormley
Review
Microbiology
Chrissy M. Leopold Wager, Eusondia Arnett, Larry S. Schlesinger
Article
Immunology
Chrissy M. Leopold Wager, Eusondia Arnett, Larry S. Schlesinger
Article
Microbiology
Chrissy M. Leopold Wager, Jordan R. Bonifacio, Jan Simper, Adrian A. Naoun, Eusondia Arnett, Larry S. Schlesinger
Summary: Mycobacterium tuberculosis exploits CREB activation in human macrophages to create an environment conducive to bacterial growth. Inhibiting the CREB signaling pathway could be an innovative approach for treating tuberculosis.
Article
Physiology
Brady J. Hurtgen, Catherine L. Ward, Chrissy M. Leopold Wager, Koyal Garg, Stephen M. Goldman, Beth E. P. Henderson, Todd O. McKinley, Sarah M. Greising, Joseph C. Wenke, Benjamin T. Corona
PHYSIOLOGICAL REPORTS
(2017)
