Article
Plant Sciences
Nuria Real, Irene Villar, Irene Serrano, Celia Guiu-Aragones, Ana Montserrat Martin-Hernandez
Summary: Resistance to cucumber mosaic virus in melon is controlled by a recessive resistance gene encoding a vacuolar protein sorting 41, which restricts the virus to bundle sheath cells. The resistant accessions show specific intravacuolar structures that colocalize with late endosomes.
Review
Cell Biology
Nagaja Capitani, Cosima T. Baldari
Summary: Membrane proteins endocytosed at the cell surface are sorted and recycled at early endosomes by multi-molecular complexes, promoting actin polymerization. Endosomal actin pools play a crucial role in cargo recycling, contributing to transport and cell polarity acquisition during the assembly of immune synapse in T cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Laura A. Hehl, Brenda A. Schulman
Summary: Tsai et al.1 and Mark et al.2 show that the E3 ligase UBR5 mediates broad regulation by selectively targeting agonist-bound nuclear hormone receptors, MYC, and other transcriptional regulators not incorporated into active gene expression complexes.
Editorial Material
Biochemistry & Molecular Biology
Yuan Xie, Minglei Zhao
Summary: Proper regulation of protein degradation is crucial for cell physiology. In the recent issue of Cell, Baek et al. revealed the assembly and disassembly mechanism of a widely present ubiquitin ligase class called CRL, through a key regulatory protein, CAND1.
Review
Oncology
Jerry Vriend, Mark W. Nachtigal
Summary: This study utilized public datasets to identify potential therapeutic targets in high-grade serous ovarian cancer by analyzing genes encoding proteins of the ubiquitin proteasome system. The differential expression of genes encoding ubiquitin ligases, ubiquitin ligase adaptors, and Cullin Ring Ligase (CRL) adaptors suggests their potential as novel therapeutic targets. The findings also indicate the involvement of CRLs in HGSOC and highlight the importance of ubiquitin proteasome pathway components in this type of cancer.
Article
Medicine, Research & Experimental
Zongang Liu, Mengnan Zhao, Xizi Jiang, Yao Zhang, Suning Zhang, Yitong Xu, Hongjiu Ren, Hongbo Su, Huanxi Wang, Xueshan Qiu
Summary: This study elucidates the role of KLHL17 in the development and progression of NSCLC using clinical samples and NSCLC cell lines. The results show that upregulated KLHL17 in NSCLC promotes the proliferation and migration of tumor by activating Ras/MAPK signaling pathway, and suggest that KLHL17 may be a novel therapeutic target for the treatment of NSCLC.
LABORATORY INVESTIGATION
(2022)
Editorial Material
Pharmacology & Pharmacy
Asad M. Taherbhoy, Danette L. Daniels
Summary: This article introduces targeted protein degradation (TPD) and proposes multiple strategies using UBR5 as an E3 ligase to drive degradation of key transcriptional targets implicated in disease.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2023)
Article
Medicine, General & Internal
Guannan Zhou, Yuanyuan Gu, Zhongyi Zhu, Hongdao Zhang, Wei Liu, Beiying Xu, Fangyue Zhou, Menglei Zhang, Keqin Hua, Ligang Wu, Jingxin Ding
Summary: This study discovered that exosomes can deliver cisplatin through clathrin-independent endocytosis, avoiding endosome trapping and evenly distributing in the cytosol. The research clarifies the mechanism of exosome-mediated drug delivery against cisplatin-resistant cancer and suggests that exosomes can be a potential nano-carrier for treating cisplatin-resistant ovarian cancer.
FRONTIERS IN MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Jan Knop, Tim Lienemann, Haifa El-Kilani, Sven Falke, Catharina Krings, Maria Sindalovskaya, Johannes Bergler, Christian Betzel, Stefan Hoth
Summary: Plant U-box armadillo repeat (PUB-ARM) ubiquitin (Ub) ligases play crucial roles in plant defense by ubiquitinating target proteins. The PUB-ARM protein SENESCENCE ASSOCIATED UBIQUITIN E3 LIGASE1 (SAUL1) can form patches at the plasma membrane related to tethering multi-vesicular bodies (MVBs) to the membrane. The structure of SAUL1 consists of two domains: a domain containing the N-terminal U-box and armadillo (ARM) repeats, and the C-terminal ARM repeat domain, which includes a positively charged groove.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Cell Biology
Jun-Ichi Sakamaki, Noboru Mizushima
Summary: Conjugation of Atg8-family proteins to phosphatidylethanolamine (PE) is important for auto-phagosome formation. PE conjugation was previously thought to be specific to Atg8 among the ubiquitin-family proteins, but our recent study found that ubiquitin is also conjugated to PE on endosomes and the vacuole. Other ubiquitin-like proteins, like NEDD8 and ISG15, can also bind to phospholipids covalently. We propose that conjugation to phospholipids could be a common feature of the ubiquitin family.
Review
Pharmacology & Pharmacy
Si-Min Qi, Jinyun Dong, Zhi-Yuan Xu, Xiang-Dong Cheng, Wei-Dong Zhang, Jiang-Jiang Qin
Summary: PROTAC technology is an effective method for degrading target proteins through the ubiquitin-proteasome system, with promising applications in cancer treatment. The first oral PROTACs have shown encouraging results in clinical trials, sparking greater enthusiasm for PROTAC research.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Immunology
Wenzheng Han, Qing Chen, Jun Cui, Yinxia Zhao, Min Li, Xiaoning Li
Summary: The study found that human RNF114 promotes VSV replication by inhibiting the type I interferon signaling pathway and demonstrated its negative regulatory role in vivo.
MICROBIAL PATHOGENESIS
(2022)
Review
Biochemistry & Molecular Biology
Rui Zhang, Shaoqing Shi
Summary: HECT-type E3 ubiquitin ligases play a vital role in controlling protein function and stability, and members of the NEDD4 family have critical roles in dysregulation of autophagy in cancer cells. This review focuses on the role of NEDD4 E3 ligases in defective autophagy in cancer cells, discussing their function, substrates, and signaling pathways, providing a basis for cancer treatment through modulation of these ligases.
MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Bjoern U. Klink, Eric Herrmann, Claudia Antoni, Lars Langemeyer, Stephan Kiontke, Christos Gatsogiannis, Christian Ungermann, Stefan Raunser, Daniel Kuemmel
Summary: This study presents a high-resolution cryogenic electron microscopy structure of the Mon1-Ccz1 complex, revealing its strong scaffold for the catalytic center and the phosphatidylinositol phosphate-binding site, providing molecular insights into endosomal Rab activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Naava Naslavsky, Steve Caplan
Summary: This Viewpoint focuses on the importance of endosomes in cells and the underlying mechanisms of their functions.
Article
Biochemistry & Molecular Biology
Ley-Hian Low, Yuh-Lit Chow, Yijia Li, Choo-Peng Goh, Ulrich Putz, John Silke, Toru Ouchi, Jason Howitt, Seong-Seng Tan
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Biochemical Research Methods
Ulrich Putz, Sophia Mah, Choo-Peng Goh, Ley-Hian Low, Jason Howitt, Seong-Seng Tan
Article
Cell Biology
Jason Howitt, Ley-Hian Low, Ulrich Putz, Anh Doan, Jenny Lackovic, Choo-Peng Goh, Jenny Gunnersen, John Silke, Seong-Seng Tan
JOURNAL OF MOLECULAR CELL BIOLOGY
(2015)
Review
Biochemistry & Molecular Biology
Jason Howitt, Andrew F. Hill
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Biotechnology & Applied Microbiology
Ulrich Sterzenbach, Ulrich Putz, Ley-Hian Low, John Silke, Seong-Seng Tan, Jason Howitt
Article
Neurosciences
S. S. Murray, A. W. Wong, J. Yang, Y. Li, U. Putz, S-S. Tan, J. Howitt
MOLECULAR NEUROBIOLOGY
(2019)
Article
Cell Biology
Mayura Wagle, Julia M. Marchingo, Jason Howitt, Seong-Seng Tan, Christopher C. Goodnow, Ian A. Parish
Article
Neurosciences
Vicki E. Hammond, Jenny M. Gunnersen, Choo-Peng Goh, Ley-Hian Low, Tomoko Hyakumura, Michelle M. Tang, Joanne M. Britto, Ulrich Putz, Jason A. Howitt, Seong-Seng Tan
Article
Neurosciences
Choo-Peng Goh, Ulrich Putz, Jason Howitt, Ley-Hian Low, Jenny Gunnersen, Nicole Bye, Cristina Morganti-Kossmann, Seong-Seng Tan
EXPERIMENTAL NEUROLOGY
(2014)
Article
Neurosciences
Choo-Peng Goh, Ley-Hian Low, Ulrich Putz, Jenny Gunnersen, Vicki Hammond, Jason Howitt, Seong-Seng Tan
NEUROSCIENCE LETTERS
(2013)
Article
Multidisciplinary Sciences
Jason Howitt, Amanda M. Gysbers, Scott Ayton, Francine Carew-Jones, Ulrich Putz, David I. Finkelstein, Glenda M. Halliday, Seong-Seng Tan
Article
Multidisciplinary Sciences
John A. Altin, Stephen R. Daley, Jason Howitt, Helen J. Rickards, Alison K. Batkin, Keisuke Horikawa, Simon J. Prasad, Keats A. Nelms, Sharad Kumar, Lawren C. Wu, Seong-Seng Tan, Matthew C. Cook, Christopher C. Goodnow
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Review
Biochemistry & Molecular Biology
Alex Mazurskyy, Jason Howitt
NEUROCHEMICAL RESEARCH
(2019)
Review
Biochemistry & Molecular Biology
Arjelle Decasa Agupitan, Paul Neeson, Scott Williams, Jason Howitt, Sue Haupt, Ygal Haupt
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)