Article
Multidisciplinary Sciences
Shuai Hu, Baiying Li, Fan Wu, Dongmei Zhu, Jan Zouhar, Caiji Gao, Tomoo Shimada, Enrique Rojo, Ikuko Hara-Nishimura, Liwen Jiang, Jinbo Shen
Summary: This study elucidates the role and mechanism of ALIX in regulating vacuolar transport of soluble proteins in plants. It is found that ALIX physically interacts with the retromer core subunits Vps26 and Vps29 and cooperatively regulates the trafficking of soluble vacuolar proteins. Depletion of ALIX disrupts the membrane recruitment of Vps26 and Vps29 and alters the endosomal localization of vacuolar sorting receptors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Sebastian Baenfer, Sophie Kutscher, Fenja Fleck, Martina Dienst, Christian Preusser, Elke Pogge von Strandmann, Ralf Jacob
Summary: In this study, we demonstrate that E-cadherin can be recruited into the membrane of extracellular vesicles through a specific domain in its cytoplasmic tail that interacts with Tsg101. This finding contributes to a better understanding of the formation and function of extracellular vesicles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Rostyslav Horbay, Ali Hamraghani, Leonardo Ermini, Sophie Holcik, Shawn T. Beug, Behzad Yeganeh
Summary: In this review, we summarize recent advances on the complex roles of sphingolipids and lysosomes in the process of extracellular vesicle (EVs) biogenesis to uptake.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Shenmin Xie, Qin Zhang, Li Jiang
Summary: Extracellular vesicles, specifically exosomes, are nanoscale membrane vesicles released by cells and have the potential to uncover unknown cellular and molecular mechanisms in intercellular communication and disease. They are effective drug delivery systems and display complexity in their formation, release, and bioengineering.
Article
Plant Sciences
Chuanliang Liu, Yonglun Zeng, Hongbo Li, Chao Yang, Wenjin Shen, Min Xu, Zhidan Xiao, Tongsheng Chen, Baiying Li, Wenhan Cao, Liwen Jiang, Marisa S. Otegui, Caiji Gao
Summary: The study identified FYVE4 as a novel plant endosomal regulator, functioning in the ESCRT pathway to regulate MVE biogenesis.
Article
Biotechnology & Applied Microbiology
Julia Radler, Dhanu Gupta, Antje Zickler, Samir EL Andaloussi
Summary: Extracellular vesicles (EVs) have become a focus of attention in the field of diagnostic and therapeutic applications for various diseases. These natural nanoparticles have favorable safety profiles and unique biodistribution capabilities, making them attractive as drug delivery modalities compared to synthetic analogs. However, their widespread use is limited by technological shortcomings and biological gaps in understanding their heterogeneity. Understanding their biogenesis is crucial for fully unlocking their therapeutic potential.
Article
Microbiology
Juan Manuel Spera, Francisco Guaimas, Cecilia Czibener, Juan Esteban Ugalde
Summary: How intracellular bacterial pathogens egress from host cells has been poorly studied. This is particularly important because this stage of the infectious cycle can have a strong impact on how the host resolves the infection. Brucella is an intracellular pathogen that infects mammals, including humans, and causes a chronic debilitating illness. Our research shows that Brucella exploits multivesicular bodies to exit host cells, and we visualized the process of egress in real time using live video microscopy. Manipulating multivesicular bodies with drugs can alter the efficiency of bacterial egress, indicating that hijacking multivesicular bodies is crucial for Brucella to initiate reinfection.
Article
Biochemistry & Molecular Biology
Takahide Matsui, Futaba Osaki, Shu Hiragi, Yuriko Sakamaki, Mitsunori Fukuda
Summary: The study demonstrates that different types of small extracellular vesicles, known as exosomes, are differentially secreted from the apical and basolateral sides of polarized epithelial cells. Two independent machineries are responsible for the polarized exosome release from epithelial cells.
Review
Biochemistry & Molecular Biology
Linda Wedemann, Felix J. Flomm, Jens B. Bosse
Summary: HCMV is a major virus causing congenital disabilities and diseases in immunocompromised patients, and the envelopment and egress processes play crucial roles in viral spread and tropism. Researchers discussed the role of virus-containing multivesicular body-like vesicles in virus egress and spread, and explored potential divergent egress pathways leading to virions of different tropisms.
MOLECULAR MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Hong Wei, Qi Chen, Li Lin, Chunli Sha, Taoqiong Li, Yueqin Liu, Xinming Yin, Yuhao Xu, Lu Chen, Wujiang Gao, Yuefeng Li, Xiaolan Zhu
Summary: Cellular communication through the exchange of biological information, mainly in the form of proteins and RNAs, is mediated by extracellular vesicles like exosomes. Understanding the molecular mechanisms of exosome secretion and cargo sorting is crucial for shedding light on their physiological and pathological functions, as well as for exploring potential therapeutic opportunities in clinical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chun-Che Tseng, Robert C. Piper, David J. Katzmann
Summary: The Endosomal Sorting Complexes Required for Transport (ESCRTs) play a crucial role in membrane remodeling during cellular processes. Recent research has uncovered the ability of Bro1, an ESCRT-associated Ub-binding protein, to coordinate ESCRT-III and Vps4-dependent intralumenal vesicle (ILV) biogenesis, as well as its involvement in upstream events such as cargo recognition.
Article
Biochemistry & Molecular Biology
Xiaohui Qiu, Yvan Campos, Diantha van de Vlekkert, Elida Gomero, Ajay C. Tanwar, Ravi Kalathur, Jason A. Weesner, Antonella Bongiovanni, Jeroen Demmers, Alessandra d'Azzo
Summary: This article reveals the mechanism of Alix homodimerization and its functions in cellular processes. The study shows that Alix can form dimers through disulfide bonds under physiological conditions and these dimers are enriched in exosomes and F-actin cytoskeleton. Furthermore, Alix plays an essential role in loading syntenin into exosomes and has different effects on cytoskeleton remodeling in its dimeric and monomeric forms.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Neurosciences
Jessica A. Lawrence, Patricia Aguilar-Calvo, Daniel Ojeda-Juarez, Helen Khuu, Katrin Soldau, Donald P. Pizzo, Jin Wang, Adela Malik, Timothy F. Shay, Erin E. Sullivan, Brent Aulston, Seung Min Song, Julia A. Callender, Henry Sanchez, Michael D. Geschwind, Subhojit Roy, Robert A. Rissman, JoAnn Trejo, Nobuyuki Tanaka, Chengbiao Wu, Xu Chen, Gentry N. Patrick, Christina J. Sigurdson
Summary: Endolysosomal defects, including the reduction of Hrs and STAM1, exacerbate synaptic derangements and accelerate neurodegeneration in prion-infected brains. Depletion of neuronal Hrs leads to increased surface levels of PrPC, contributing to the rapidly advancing disease through neurotoxic signaling. The findings highlight the importance of proteostatic pathways and synaptic integrity in prion diseases.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Cell Biology
Satoru Higashi, Tomohiko Makiyama, Hiroshi Sakane, Satoru Nogami, Hiromichi Shirataki
Summary: In this study, a novel regulator called gamma-taxilin was found to be involved in Hook1-mediated endosomal sorting. Gamma-taxilin negatively regulates the sorting of cargo proteins by interfering with the interactions between Hook1 and the cargo proteins.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Sudeep Banjade, Lu Zhu, Jeffrey R. Jorgensen, Sho W. Suzuki, Scott D. Emr
Summary: This study investigates the mechanism of action of ESCRTs in yeast vacuoles, finding that multivalent interactions between ESCRT-0 and polyubiquitin are critical for substrate recognition and proposing that self-assembly of early ESCRTs induces condensation and assembly on membranes.
Article
Cell Biology
Shrawan Kumar Mageswaran, Natalie K. Johnson, Greg Odorizzi, Markus Babst
MOLECULAR BIOLOGY OF THE CELL
(2015)
Article
Biochemistry & Molecular Biology
Stephen D. Carter, Shrawan K. Mageswaran, Zachary J. Farino, Joao I. Mamede, Catherine M. Oikonomou, Thomas J. Hope, Zachary Freyberg, Grant J. Jensen
JOURNAL OF STRUCTURAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Mohammed Kaplan, Poorna Subramanian, Debnath Ghosal, Catherine M. Oikonomou, Sahand Pirbadian, Ruth Starwalt-Lee, Shrawan Kumar Mageswaran, Davi R. Ortega, Jeffrey A. Gralnick, Mohamed Y. El-Naggar, Grant J. Jensen
Article
Microbiology
Eleonora Aquilini, Marta Mendonca Cova, Shrawan Kumar Mageswaran, Nicolas Dos Santos Pacheco, Daniela Sparvoli, Diana Marcela Penarete-Vargas, Rania Najm, Arnault Graindorge, Catherine Suarez, Marjorie Maynadier, Laurence Berry-Sterkers, Serge Urbach, Pilar Ruga Fahy, Amandine N. Guerin, Boris Striepen, Jean-Francois Dubremetz, Yi-Wei Chang, Aaron P. Turkewitz, Maryse Lebrun
Summary: The study reveals that the mechanism of rhoptry secretion in Apicomplexa shares structural and genetic elements with ciliates, indicating a conserved secretion system adapted for defense in free-living unicellular eukaryotes and host cell injection in intracellular parasites. The formation of a rosette-shaped intramembranous particle embedded in the parasite apex plasma membrane is crucial for rhoptry exocytosis, which depends on non-discharge proteins conserved in Ciliata, Dinoflagellata, and Apicomplexa within the superphylum Alveolata.
NATURE MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shrawan Kumar Mageswaran, Wei Yuan Yang, Yogaditya Chakrabarty, Catherine M. Oikonomou, Grant J. Jensen
Summary: The study combines live-cell microscopy, correlative light and electron microscopy, and cryo-ET to investigate shedding in response to plasma membrane damage in HeLa cells, revealing filopodia-like protrusions as scaffolds enriched at damage sites. The shedding process involves F-actin dynamics, myosin-1a, and vacuolar protein sorting 4B, indicating a more complex mechanism than current models of vesiculation. The findings suggest a conserved mechanism with constitutive shedding in enterocytes and the potential for adapting the workflow to study other damage response pathways and dynamic cellular events.
Article
Multidisciplinary Sciences
Shrawan Kumar Mageswaran, Amandine Guerin, Liam M. Theveny, William David Chen, Matthew Martinez, Maryse Lebrun, Boris Striepen, Yi-Wei Chang
Summary: The study reveals the conserved architecture and mechanism of rhoptries from two different apicomplexan parasites, providing insights into how these important parasites deliver effectors into host cells.
NATURE COMMUNICATIONS
(2021)
Review
Parasitology
Liam M. Theveny, Shrawan Kumar Mageswaran, William David Chen, Matthew Martinez, Amandine Guerin, Yi-Wei Chang
Summary: Cryo-electron tomography (cryo-ET) is a cryo-EM approach that allows 3D imaging of cellular structures with molecular resolution. Advanced technologies and workflows have improved the information yield from cellular samples, while sample-thinning techniques such as cryo-FIB milling have expanded the scope of cryo-ET. Cryo-ET has become more versatile and capable of transforming our understanding of parasite biology.
TRENDS IN PARASITOLOGY
(2022)
Correction
Multidisciplinary Sciences
Shrawan Kumar Mageswaran, Amandine Guerin, Liam M. Theveny, William David Chen, Matthew Martinez, Maryse Lebrun, Boris Striepen, Yi-Wei Chang
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Matthew Martinez, William David Chen, Marta Mendonca Cova, Petra Molnar, Shrawan Kumar Mageswaran, Amandine Guerin, Audrey R. Odom John, Maryse Lebrun, Yi-Wei Chang
Summary: In this study, cryo-electron tomography was used to investigate the ultrastructure of the rhoptry secretion system in Plasmodium falciparum. The presence of a rhoptry secretory apparatus (RSA) and a morphologically distinct apical vesicle was identified, and different conformations of the RSA structure corresponding to different rhoptry organizations were revealed. These findings provide new insights into the process of rhoptry secretion and indicate the regulatory role of the apical vesicle in host invasion by apicomplexan parasites.
NATURE MICROBIOLOGY
(2022)