Article
Biochemistry & Molecular Biology
Jenny Serra-Vinardell, Maxwell B. Sandler, Raffaella De Pace, Javier Manzella-Lapeira, Antony Cougnoux, Keyvan Keyvanfar, Wendy J. Introne, Joseph A. Brzostowski, Michael E. Ward, William A. Gahl, Prashant Sharma, May Christine C. Malicdan
Summary: Chediak-Higashi syndrome (CHS) is a rare autosomal recessive disorder caused by mutations in the LYST gene. This study shows that LYST-deficient human neuronal models exhibit lysosome depletion and hyperelongated tubules protruding from autolysosomes, which is also observed in neurons derived from CHS patients' induced pluripotent stem cells (iPSCs). The results suggest that LYST plays a crucial role in autophagic lysosome reformation (ALR) by ensuring the correct fission of autolysosome tubules and being recruited to the lysosome membrane.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Cell Biology
K. Adam Bohnert, Alyssa E. Johnson
Summary: Lysosomes are essential organelles in cells, with both vesicular and tubular forms. Tubular lysosomes may have unique functions that are critical to cell behavior, function, and viability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Erika Garay, Nils Schuth, Alessandra Barbanente, Carlos Tejeda-Guzman, Daniele Vitone, Beatriz Osorio, Adam H. Clark, Maarten Nachtegaal, Michael Haumann, Holger Dau, Alberto Vela, Fabio Arnesano, Liliana Quintanar, Fanis Missirlis
Summary: This study reveals that zinc stores in Drosophila melanogaster are regulated by tryptophan consumption. The tryptophan metabolite kynurenine induces the formation of zinc storage granules, thus modulating zinc status of the insect.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Medicine, General & Internal
Jose Alcides Almeida de Arruda, Sebastiao Silverio Sousa-Neto, Lucas Guimaraes Abreu, Lauren Frenzel Schuch, Vinicius Gomes Souza, Thainara Vitoria Lima Alves, Barbara Martins-Andrade, Sameep S. Shetty, Joao Luiz Gomes Carneiro Monteiro, Elismauro Francisco Mendonca, Ricardo Alves Mesquita, Gerhilde Callou
Summary: This article summarizes the oral manifestations of individuals with Chediak-Higashi syndrome (CHS), including periodontal disease, oral mucosal ulceration, gingival/labial abscess, and periodontal abscess. Oral rehabilitation such as dental implants may be needed due to the poor prognosis of periodontal therapy. Oral health providers should be aware of these manifestations and provide special care and prophylaxis.
DM DISEASE-A-MONTH
(2023)
Article
Engineering, Environmental
Zishuang Shao, Paul Guagliardo, Haibo Jiang, Wen-Xiong Wang
Summary: This study utilized high-resolution nanoscale secondary ion mass spectrometry and scanning electron microscopy to visualize the subcellular localization and potential toxicity effects of AgNPs in oyster gill filaments. The findings demonstrated an endocytosis-based entry of AgNPs into cells and identified AgNP-induced autophagy in oyster gill cells. Additionally, the study revealed the transport and sequestration roles of different hemocytes in AgNP distribution.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiao-Wei Pang, Yun-Hui Chu, Luo-Qi Zhou, Man Chen, Yun-Fan You, Yue Tang, Sheng Yang, Hang Zhang, Jun Xiao, Gang Deng, Wei Wang, Ke Shang, Chuan Qin, Dai-Shi Tian
Summary: This study investigated the specific role of Trem2 in chronic cerebral hypoperfusion. The results showed that Trem2 deficiency alleviated microglial phagocytosis and activation of the autophagic-lysosomal pathway, leading to microglial polarization towards anti-inflammatory and homeostatic phenotypes. These findings suggest that Trem2 could be a promising target for the treatment of chronic cerebral hypoperfusion.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Medical Laboratory Technology
Ruimin Li, Yiping Wu, Xiaofang Zhang, Lihua Wang, Yungang Zhang, Heng Xiao
Summary: The aim of the study was to improve the understanding of complex karyotype acute mixed cell leukemia containing pseudo Chediak-Higashi granules. A case of acute mixed cell leukemia resembling AML1-ETO positive acute myeloid leukemia was reported and analyzed through morphological, immunophenotypic, and cytogenetic tests.
CLINICAL LABORATORY
(2023)
Article
Cell Biology
Halim aKusumaatmaja, Alexander May, Roland L. Knorr
Summary: Protein-rich droplets, formed by phase separation and interacting with membrane-bound organelles, play crucial roles in intracellular organization and function. The physical mechanisms of droplet-membrane interactions and the novel reshaping mechanism of membrane scaffolding by liquids provide insights into the complex and elastic wetting phenomena in cells. These interactions link phase separation with mechanosensitivity and explain key cellular structures observed during embryogenesis, autophagy, and at synapses.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Oncology
Almudena Fernandez, Masahiro Hayashi, Gema Garrido, Andrea Montero, Ana Guardia, Tamio Suzuki, Lluis Montoliu
Summary: Oculocutaneous albinism (OCA) is a common presentation of albinism, affecting pigmentation and vision, with syndromic and non-syndromic types. Syndromic OCA may be more severe and associated with additional systemic consequences.
PIGMENT CELL & MELANOMA RESEARCH
(2021)
Article
Cell Biology
Ramona Lattao, Helene Rangone, Salud Llamazares, David M. Glover
Summary: Fruit fly Mauve suppresses vesicle fusion events with lipid droplets during yolk granules formation and opposes fusion-promoting Rab5. It also interacts closely with LDs component Minispindles/Ch-TOG, and changes in Minispindles levels can affect microtubule nucleation from centrosomes.
DEVELOPMENTAL CELL
(2021)
Article
Multidisciplinary Sciences
Shinji Sasaki, Toshio Watanabe, Takayuki Ibi, Kiyotoshi Hasegawa, Yoichi Sakamoto, Shunsuke Moriwaki, Kazuhito Kurogi, Atsushi Ogino, Takanori Yasumori, Hiroyuki Wakaguri, Eiji Muraki, Youko Miki, Yuichi Yoshida, Yoshinobu Inoue, Ichiro Tabuchi, Ken Iwao, Taichi Arishima, Keisuke Kawashima, Manabu Watanabe, Sumio Sugano, Yoshikazu Sugimoto, Yutaka Suzuki
Summary: The intensive use of a few elite sires in cattle has led to an increased risk of deleterious recessive traits. By analyzing SNP array data and exome sequencing, researchers identified deleterious recessive haplotypes in Japanese Black cattle, some of which affect embryonic survival. The study found specific candidate mutations in genes like IARS, CDC45, and CLDN16 associated with these deleterious haplotypes.
SCIENTIFIC REPORTS
(2021)
Review
Neurosciences
Martin Lang, Peter P. Pramstaller, Irene Pichler
Summary: Living organisms need to adapt and handle stress, and mitochondria and lysosomes play a key role in regulating energy and nutrient availability within cells. Dysfunction in these mechanisms can lead to pathologies, such as Parkinson's disease. Mitochondrial dysfunction and defects in the autophagy-lysosomal pathway have been observed in PD patients and models, and the MiT pathway, which regulates cellular homeostasis, could be a potential therapeutic target against PD.
MOLECULAR NEURODEGENERATION
(2022)
Article
Neurosciences
Hailan He, Xiaoshuang Cao, Fei Yin, Tenghui Wu, Tobias Stauber, Jing Peng
Summary: Vesicular chloride/proton exchangers of the CLC family are crucial for the endosomal-lysosomal pathway, and dysfunction may lead to severe disorders. A study reported a novel variant of CLCN6 associated with West syndrome, providing clinical and functional evidence for the link. The research shed light on the involvement of autophagic-lysosomal dysfunction in the pathogenesis of West syndrome.
MOLECULAR NEUROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Zhibo Si, Yajuan Zheng, Jing Zhao
Summary: Age-related macular degeneration (AMD) is a common cause of vision loss in the elderly. It is characterized by the formation of Drusen in dry AMD and cell proliferation and choroidal angiogenesis in wet AMD. The retinal pigment epithelium (RPE) plays a crucial role in the development of AMD, with outer segment phagocytosis and autophagy being important processes for RPE cell renewal and protection against oxidative stress damage. However, impaired phagocytosis and autophagy in RPE cells can lead to AMD progression. Understanding the roles of RPE cell phagocytosis and autophagy in AMD pathogenesis can help identify potential therapeutic targets to prevent irreversible RPE and photoreceptor cell death and protect against AMD.
Article
Pathology
Moeinadin Safavi, Nima Parvaneh
Summary: Chediak-Higashi syndrome (CHS) is a disorder caused by dysfunction of lysosomal trafficking, leading to hypopigmentation, bleeding tendencies, neurological symptoms, and NK cell dysfunction. CHS can be one of the underlying causes of HLH.
FETAL AND PEDIATRIC PATHOLOGY
(2023)
Editorial Material
Cell Biology
Nilay Nandi, Helmut Kramer
Article
Urology & Nephrology
Qifei Sun, Yipin Wu, Sima Jonusaite, John M. Pleinis, John M. Humphreys, Haixia He, Jeffrey N. Schellinger, Radha Akella, Drew Stenesen, Helmut Kramer, Elizabeth J. Goldsmith, Aylin R. Rodan
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2018)
Article
Biology
Andrew T. Moehlman, Amanda K. Casey, Kelly Servage, Kim Orth, Helmut Kraemer
Article
Neurosciences
Annie Vu, Tyler Humphries, Sean Vogel, Adam Haberman
MOLECULAR AND CELLULAR NEUROSCIENCE
(2018)
Article
Cell Biology
Paula Kurtz, Amanda E. Jones, Bhavana Tiwari, Nichole Link, Annika Wylie, Charles Tracy, Helmut Kramer, John M. Abrams
MOLECULAR BIOLOGY OF THE CELL
(2019)
Editorial Material
Cell Biology
Michael Buszczak, Helmut Kramer
DEVELOPMENTAL CELL
(2019)
Article
Cell Biology
Lauren K. Tyra, Nilay Nandi, Charles Tracy, Helmut Kramer
DEVELOPMENTAL CELL
(2020)
Article
Cell Biology
Jian Zhang, Charles Tracy, Chandrashekhar Pasare, Jinsheng Zeng, Helmut Kraemer
Article
Biology
Nilay Nandi, Zuhair Zaidi, Charles Tracy, Helmut Kramer
Summary: This study identified the mechanisms regulating neuronal autophagy. It was found that the PPM-type phosphatase Nil counterbalances the phosphorylation of Acinus-serine(437) and controls the level of neuronal autophagy. It was also discovered that exposure to Cd2+ increases the phosphorylation of Acinus-serine(437), leading to increased autophagy and protection against Cd2+-induced cytotoxicity.
Article
Education, Scientific Disciplines
David Lopatto, Anne G. Rosenwald, Rebecca C. Burgess, Catherine Silver Key, Melanie Van Stry, Matthew Wawersik, Justin R. DiAngelo, Amy T. Hark, Matthew Skerritt, Anna K. Allen, Consuelo Alvarez, Sara Anderson, Cindy Arrigo, Andrew Arsham, Daron Barnard, James E. J. Bedard, Indrani Bose, John M. Braverman, Martin G. Burg, Paula Croonquist, Chunguang Du, Sondra Dubowsky, Heather Eisler, Matthew A. Escobar, Michael Foulk, Thomas Giarla, Rivka L. Glaser, Anya L. Goodman, Yuying Gosser, Adam Haberman, Charles Hauser, Shan Hays, Carina E. Howell, Jennifer Jemc, Christopher J. Jones, Lisa Kadlec, Jacob D. Kagey, Kimberly L. Keller, Jennifer Kennell, Adam J. Kleinschmit, Melissa Kleinschmit, Nighat P. Kokan, Olga Ruiz Kopp, Meg M. Laakso, Judith Leatherman, Lindsey J. Long, Mollie Manier, Juan C. Martinez-Cruzado, Luis F. Matos, Amie Jo McClellan, Gerard McNeil, Evan Merkhofer, Vida Mingo, Hemlata Mistry, Elizabeth Mitchell, Nathan T. Mortimer, Jennifer Leigh Myka, Alexis Nagengast, Paul Overvoorde, Don Paetkau, Leocadia Paliulis, Susan Parrish, Stephanie Toering Peters, Mary Lai Preuss, James V. Price, Nicholas A. Pullen, Catherine Reinke, Dennis Revie, Srebrenka Robic, Jennifer A. Roecklein-Canfield, Michael R. Rubin, Takrima Sadikot, Jamie Siders Sanford, Maria Santisteban, Kenneth Saville, Stephanie Schroeder, Christopher D. Shaffer, Karim A. Sharif, Diane E. Sklensky, Chiyedza Small, Sheryl Smith, Rebecca Spokony, Aparna Sreenivasan, Joyce Stamm, Rachel Sterne-Marr, Katherine C. Teeter, Justin Thackeray, Jeffrey S. Thompson, Norma Velazquez-Ulloa, Cindy Wolfe, James Youngblom, Brian Yowler, Leming Zhou, Janie Brennan, Jeremy Buhler, Wilson Leung, Sarah C. R. Elgin, Laura K. Reed
Summary: This study investigates the impact of student attitudes on their success in a course-based undergraduate research experience. The findings suggest that students' attitudes, particularly their positive beliefs about science, play a significant role in achieving positive learning outcomes.
JOURNAL OF MICROBIOLOGY & BIOLOGY EDUCATION
(2022)
Article
Multidisciplinary Sciences
Amanda K. Casey, Hillery F. Gray, Suneeta Chimalapati, Genaro Hernandez, Andrew T. Moehlman, Nathan Stewart, Hazel A. Fields, Burak Gulen, Kelly A. Servage, Karoliina Stefanius, Aubrie Blevins, Bret M. Evers, Helmut Kramer, Kim Orth
Summary: Protein homeostasis is crucial for cellular health, and imbalanced unfolded protein response (UPR) can lead to inflammation and cellular damage. The enzyme filamentation induced by cyclic-AMP (Fic) modulates UPR response by posttranslational modification of binding immunoglobulin protein (BiP). Loss of Fic affects vision, UPR activation, and stress recovery. This study highlights the importance of Fic-mediated AMPylation in the mammalian pancreas during physiological stress.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Shashank Shekhar, Andrew T. Moehlman, Brenden Park, Michael Ewnetu, Charles Tracy, Iris Titos, Krzysztof Pawlowski, Vincent S. Tagliabracci, Helmut Kramer
Summary: The study reveals that Allnighter (Aln), a pseudokinase, plays a crucial role in maintaining normal sleep and neuronal structural plasticity in Drosophila photoreceptors by regulating proteostasis. Aln protein is secreted by different neurons but not expressed in photoreceptors, yet it is retrogradely endocytosed by photoreceptors. Inhibition of photoreceptor synaptic release reduces Aln levels in lamina neurons, indicating the involvement of secreted Aln in a feedback loop. Moreover, aln mutants exhibit reduced night time sleep, suggesting a molecular link between dysregulated proteostasis and sleep, which are characteristics of ageing and neurodegenerative diseases.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Nilay Nandi, Charles Tracy, Helmut Kramer
Summary: Understanding the phosphorylation status of autophagy-related proteins is crucial, with current research focusing more on kinases rather than phosphatases. Genetic screens and proteomic approaches are effective tools for identifying candidate phosphatases, but further experiments are needed to confirm their direct roles.
Article
Education, Scientific Disciplines
David Lopatto, Anne G. Rosenwald, Justin R. DiAngelo, Amy T. Hark, Matthew Skerritt, Matthew Wawersik, Anna K. Allen, Consuelo Alvarez, Sara Anderson, Cindy Arrigo, Andrew Arsham, Daron Barnard, Christopher Bazinet, James E. J. Bedard, Indrani Bose, John M. Braverman, Martin G. Burg, Rebecca C. Burgess, Paula Croonquist, Chunguang Du, Sondra Dubowsky, Heather Eisler, Matthew A. Escobar, Michael Foulk, Emily Furbee, Thomas Giarla, Rivka L. Glaser, Anya L. Goodman, Yuying Gosser, Adam Haberman, Charles Hauser, Shan Hays, Carina E. Howell, Jennifer Jemc, M. Logan Johnson, Christopher J. Jones, Lisa Kadlec, Jacob D. Kagey, Kimberly L. Keller, Jennifer Kennell, S. Catherine Silver Key, Adam J. Kleinschmit, Melissa Kleinschmit, Nighat P. Kokan, Olga Ruiz Kopp, Meg M. Laakso, Judith Leatherman, Lindsey J. Long, Mollie Manier, Juan C. Martinez-Cruzado, Luis F. Matos, Amie Jo McClellan, Gerard McNeil, Evan Merkhofer, Vida Mingo, Hemlata Mistry, Elizabeth Mitchell, Nathan T. Mortimer, Debaditya Mukhopadhyay, Jennifer Leigh Myka, Alexis Nagengast, Paul Overvoorde, Don Paetkau, Leocadia Paliulis, Susan Parrish, Mary Lai Preuss, James V. Price, Nicholas A. Pullen, Catherine Reinke, Dennis Revie, Srebrenka Robic, Jennifer A. Roecklein-Canfield, Michael R. Rubin, Takrima Sadikot, Jamie Siders Sanford, Maria Santisteban, Kenneth Saville, Stephanie Schroeder, Christopher D. Shaffer, Karim A. Sharif, Diane E. Sklensky, Chiyedza Small, Mary Smith, Sheryl Smith, Rebecca Spokony, Aparna Sreenivasan, Joyce Stamm, Rachel Sterne-Marr, Katherine C. Teeter, Justin Thackeray, Jeffrey S. Thompson, Stephanie Toering Peters, Melanie Van Stry, Norma Velazquez-Ulloa, Cindy Wolfe, James Youngblom, Brian Yowler, Leming Zhou, Janie Brennan, Jeremy Buhler, Wilson Leung, Laura K. Reed, Sarah C. R. Elgin
JOURNAL OF MICROBIOLOGY & BIOLOGY EDUCATION
(2020)
Article
Multidisciplinary Sciences
Drew Stenesen, Andrew T. Moehlman, Jeffrey N. Schellinger, Aylin R. Rodan, Helmut Kramer
SCIENTIFIC REPORTS
(2019)