Article
Biochemistry & Molecular Biology
Sung Yun Park, Nadine Muschalik, Jessica Chadwick, Sean Munro
Summary: The Golgi is a crucial sorting station in the secretory pathway, responsible for receiving different types of transport vesicles. Golgin proteins have been proposed to direct vesicles to the Golgi, with differences in cargo captured by each golgin in different tissues. While individual golgins are not essential for cell viability, the deletion of multiple golgins leads to loss of viability.
Article
Cell Biology
Viktoria Szentgyoergyi, Anne Spang
Summary: Cargo delivery in cellular organelles relies on the fusion of vesicles with the help of tethering factors. Recent studies show that tethers play a significant role in membrane fusion, and the discovery of novel tether FERARI complex has changed our understanding of cargo transport.
JOURNAL OF CELL SCIENCE
(2023)
Review
Plant Sciences
Yutaro Shimizu, Tomohiro Uemura
Summary: Membrane trafficking is crucial for organelle maintenance and function, and the TGN plays a critical role as a sorting station. Recent studies have identified distinct subdomains of the TGN for different trafficking pathways.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Cell Biology
Tien Phan-Everson, Fred Etoc, Shu Li, Samuel Khodursky, Anna Yoney, Ali H. Brivanlou, Eric D. Siggia
Summary: The research discovered that in human embryos, NOGGIN and BMP4 are secreted into different extracellular spaces, with NOGGIN inhibiting BMP4's action. Experiments with apically imposed microfluidic flow showed that NOGGIN travels in the apical extracellular space, while co-localization analysis detailed the endocytotic route that trafficked NOGGIN to the basolateral intercellular space where BMP4 receptors were located.
DEVELOPMENTAL CELL
(2021)
Article
Oncology
Khalisah L. Zulkefli, Ismail S. Mahmoud, Nicholas A. Williamson, Prajakta Kulkarni Gosavi, Fiona J. Houghton, Paul A. Gleeson
Summary: Rab30 is primarily localized to the TGN and recycling endosomes, playing a role in retrograde trafficking and maintaining endosomal-TGN organization. Interaction with Arf1 and Arf4 suggests association with tubular carriers arising from recycling endosomes.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Plant Sciences
Fangfang Niu, Changyang Ji, Zizhen Liang, Rongfang Guo, Yixuan Chen, Yonglun Zeng, Liwen Jiang
Summary: ADP-ribosylation factor (ARF) family proteins play a central role in regulating vesicular traffic and organelle structures in eukaryotes. This study characterized the function of a unique ARF protein, ARFD1B, in Arabidopsis. It was found that ARFD1B is required for cell plate formation, maintenance of Golgi morphology, and plant growth in Arabidopsis by interacting with COAT PROTEIN COMPLEX I components.
Review
Cell Biology
Petia Adarska, Luis Wong-Dilworth, Francesca Bottanelli
Summary: Molecular switches of the ADP-ribosylation factor (ARF) GTPase family play crucial roles in coordinating intracellular trafficking, including membrane deformation, cargo sorting, and recruitment of downstream coat proteins and effectors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Ying-Yi Li, Kazuyuki Kuroki, Tetsuro Shimakami, Kazuhisa Murai, Kazunori Kawaguchi, Takayoshi Shirasaki, Kouki Nio, Saiho Sugimoto, Tomoki Nishikawa, Hikari Okada, Noriaki Orita, Hideo Takayama, Ying Wang, Phuong Doan Thi Bich, Astuya Ishida, Sadahiro Iwabuchi, Shinichi Hashimoto, Takeshi Shimaoka, Noriko Tabata, Miho Watanabe-Takahashi, Kiyotaka Nishikawa, Hiroshi Yanagawa, Motoharu Seiki, Kouji Matsushima, Taro Yamashita, Shuichi Kaneko, Masao Honda
Summary: Through single-cell transcriptome analysis, we have found that DOCK11 plays a crucial role in the persistence of hepatitis B virus (HBV) infection. Our experiments have demonstrated that DOCK11 is functionally associated with the retrograde trafficking process of HBV, helping it avoid lysosomal degradation and maintain covalently closed circular viral DNA (cccDNA). Therefore, DOCK11 is a potential therapeutic target to prevent persistent HBV infection.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jie Jia, Xihua Yue, Lianhui Zhu, Shuaiyang Jing, Yijing Wang, Bopil Gim, Yi Qian, Intaek Lee
Summary: The KDEL receptor undergoes a complex recycling pathway through Golgi and perinuclear recycling endosomes when expressed on the cell surface, preferring clathrin-mediated endocytic pathway and transport carriers. This suggests that the KDEL receptor may function as a bona fide cell surface receptor with a well-defined trafficking itinerary through endolysosomal compartments.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Maika S. Deffieu, Ieva Cesonyte, Francois Delalande, Gaelle Boncompain, Cristina Dorobantu, Eli Song, Vincent Lucansky, Aurelie Hirschler, Sarah Cianferani, Franck Perez, Christine Carapito, Raphael Gaudin
Summary: The study combined RUSH and eRUSH methods and identified Rab7-harboring vesicles as important intermediates in the Golgi-to-plasma membrane transport of TfR. Rab7 was found to transiently associate with neosynthetic TfR-containing post-Golgi vesicles before dissociation before fusion with the plasma membrane, revealing the diversity of secretory vesicles.
Article
Cell Biology
Xiuping Sun, Divyanshu Mahajan, Bing Chen, Zhiwei Song, Lei Lu
Summary: The mechanism of Golgi localization of Golgi glycosyltransferases has been found to be primarily through retention rather than retrieval. The study also revealed the contributions of different regions of ST6GAL1 to Golgi retention, showing that the N-terminal cytosolic tail and transmembrane domain can act as Golgi export signals.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Nan Liu, Kai Liu, Chonglin Yang
Summary: WDR91 is identified as an important factor in retromer-dependent recycling, promoting the interaction of Rab7 with SNX-retromer components and facilitating the formation of the endosomal retrieval subdomain.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Joanne Watson, Harriet R. Ferguson, Rosie M. Brady, Jennifer Ferguson, Paul Fullwood, Hanyi Mo, Katherine H. Bexley, David Knight, Gareth Howell, Jean-Marc Schwartz, Michael P. Smith, Chiara Francavilla
Summary: In this study, the authors reveal the recruitment of FGFR signaling partners proximal to recycling endosomes using the SRP approach. They demonstrate that FGFR2b, stimulated by its recycling ligand FGF10, activates mTOR-dependent signaling and ULK1 at the recycling endosomes, leading to autophagy suppression and cell survival. This highlights the importance of RTK recycling in regulating cell fate and suggests a therapeutically targetable vulnerability in ligand-responsive cancer cells.
NATURE COMMUNICATIONS
(2022)
Article
Virology
Bailey S. Mosher, Heather L. Fulkerson, Tori Boyle, Liudmila S. Chesnokova, Stephen J. Cieply, Andrew D. Yurochko
Summary: Human cytomegalovirus (HCMV) infection causes severe and often fatal disease in the immunocompromised. By uncovering the unique pathways used by the virus to infect key cells, such as monocytes, responsible for dissemination and persistence, we provide new potential targets for therapeutic intervention.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Roya Mehrasa, Ileana Cristea, Cecilie Bredrup, Eyvind Rodahl, Ove Bruland
Summary: The ATRAID factor was discovered in HL60 cells and has multiple mRNA isoforms, but the proteins have not been fully characterized. In transfected cells expressing Myc-Flag-tagged ATRAID Isoforms A, B, and C, Iso C was highly expressed, Iso A was lowly expressed due to rapid degradation, and Iso B's predicted protein was not detected. Iso C mainly existed in a N-glycosylated form. Subcellular fractionation experiments showed that Iso C localized to membranous and nuclear fractions, while immunofluorescence analysis revealed its location close to the plasma membrane, mainly in cytoplasmic vesicles and the Golgi area. The study confirmed the colocalization of Iso C with endosomal/lysosomal markers LAMP1 and LAMP2 to some extent. Furthermore, ATRAID was shown to co-localize with RAB11, which is involved in regulating vesicular trafficking.
Article
Cell Biology
Prajakta Gosavi, Fiona J. Houghton, Paul J. McMillan, Eric Hanssen, Paul A. Gleeson
JOURNAL OF CELL SCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Jenny Chia, Jade Louber, Isabelle Glauser, Shirley Taylor, Greg T. Bass, Steve K. Dower, Paul A. Gleeson, Anne M. Verhagen
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Review
Biochemistry & Molecular Biology
Jing Zhi A. Tan, Paul A. Gleeson
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2019)
Article
Biochemistry & Molecular Biology
Jing Zhi A. Tan, Paul A. Gleeson
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Cell Biology
Christian Makhoul, Prajakta Gosavi, Regina Duffield, Bronwen Delbridge, Nicholas A. Williamson, Paul A. Gleeson
MOLECULAR BIOLOGY OF THE CELL
(2019)
Review
Cell Biology
Jing Zhi Anson Tan, Paul Anthony Gleeson
Review
Biochemistry & Molecular Biology
Prajakta Kulkarni-Gosavi, Christian Makhoul, Paul A. Gleeson
Article
Cell Biology
Wei Hong Toh, Jade Louber, Ismail S. Mahmoud, Jenny Chia, Greg T. Bass, Steve K. Dower, Anne M. Verhagen, Paul A. Gleeson
JOURNAL OF CELL SCIENCE
(2020)
Review
Biochemistry & Molecular Biology
Xiao Peng Lin, Justine D. Mintern, Paul A. Gleeson
Article
Cell Biology
Lou Fourriere, Ellie Hyun-Jung Cho, Paul A. Gleeson
Summary: This study examined the segregation of BACE1 and APP in the Golgi apparatus using high-resolution microscopy techniques. The results showed that BACE1 and APP are segregated throughout the Golgi stack, while the transferrin receptor colocalizes with BACE1 but not APP.
Article
Biochemistry & Molecular Biology
Fiona J. Houghton, Christian Makhoul, Ellie Hyun-Jung Cho, Nicholas A. Williamson, Paul A. Gleeson
Summary: There is a highly organized complex of small G proteins on TGN membranes, with Arl5b being one of its members, and Arl5b is required for TGN recruitment of ACBD3.
Editorial Material
Biochemistry & Molecular Biology
Akira Kikuchi, Zengyi Chang, Paul A. Gleeson
Article
Cell Biology
Andreas Pannek, Janine Becker-Gotot, Steven K. Dower, Anne M. Verhagen, Paul A. Gleeson
Summary: This study investigates the endocytic trafficking pathway of FcRn-albumin in primary vascular endothelial cells, and finds that it is mainly internalized through fluid-phase macropinocytosis and co-localizes with FcRn in acidic endosomal structures, before being transported to the plasma membrane through FcRn-positive tubules.
JOURNAL OF CELL SCIENCE
(2023)
Article
Cell Biology
Jingqi Wang, Maciej Daniszewski, Marlene M. Hao, Damian Hernandez, Alice Pebay, Paul A. Gleeson, Lou Fourriere
Summary: This study investigates the secretory pathways within dendrites of human neurons. It finds that the entire Golgi apparatus transiently translocates into dendrites during early neuronal development. In mature neurons, dynamic Golgi structures are transported along dendrites, showing bidirectional movement. This study reveals the presence of dynamic, functional Golgi structures in dendrites and provides insights into dendrite trafficking in human neurons.
Article
Medicine, Research & Experimental
Janine Becker-Gotot, Mirjam Meissner, Vadim Kotov, Blanca Jurado-Mestre, Andrea Maione, Andreas Pannek, Thilo Albert, Chrystel Flores, Frank A. Schildberg, Paul A. Gleeson, Birgit M. Reipert, Johannes Oldenburg, Christian Kurts
Summary: A major complication of hemophilia A therapy is the development of inhibitors that neutralize coagulation factor VIII (FVIII). Immune tolerance induction therapy (ITI) can help eradicate these inhibitors, but its success is unpredictable. This study found that programmed death (PD) ligand 1-expressing regulatory T cells (Tregs) play a role in maintaining immune tolerance against FVIII. ITI induces FVIII-specific PD-L1+ Tregs and reengages the removal of inhibitor-forming B cells. These findings have implications for monitoring ITI success and treating hemophilia A patients.
JOURNAL OF CLINICAL INVESTIGATION
(2022)