期刊
TRAFFIC
卷 12, 期 10, 页码 1383-1393出版社
WILEY
DOI: 10.1111/j.1600-0854.2011.01247.x
关键词
apical transport; axonal outgrowth; cell polarity; knockout mouse; lysosomal exocytosis; SNARE
类别
资金
- Naito Foundation
- Takeda Science Foundation
- Japanese Ministry of Education, Culture, Sports, Science and Technology
- Science and Technology Foundation of Japan (JSTF)
- Life Science Foundation of Japan
- Kao Foundation for Arts and Sciences
- Inamori Foundation
- Ichiro Kanehara Foundation
- Grants-in-Aid for Scientific Research [21390050, 23591347, 23132502] Funding Source: KAKEN
VAMP7 or tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) has been proposed to regulate apical transport in polarized epithelial cells, axonal transport in neurons and lysosomal exocytosis. To investigate the function of VAMP7 in vivo, we generated VAMP7 knockout mice. Here, we show that VAMP7 knockout mice are indistinguishable from control mice and display a similar localization of apical proteins in the kidney and small intestine and a similar localization of axonal proteins in the nervous system. Neurite outgrowth of cultured mutant hippocampal neurons was reduced in mutant neurons. However, lysosomal exocytosis was not affected in mutant fibroblasts. Our results show that VAMP7 is required in neurons to extend axons to the full extent. However, VAMP7 does not seem to be required for epithelial cell polarity and lysosomal exocytosis.
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