Article
Cell Biology
He-Yen Chou, Yi-Tang Lee, Yuchieh Jay Lin, Jung-Kun Wen, Wen-Hsin Peng, Pei-Lien Hsieh, Shu-Yu Lin, Chin-Chun Hung, Guang-Chao Chen
Summary: Macroautophagy/autophagy is an evolutionarily conserved intracellular pathway for the degradation of cytoplasmic materials. PTPN9 plays a crucial role in regulating the biogenesis of autophagosomes by modulating the phosphorylation status of VTI1B. The study highlights the importance of PTPN9 in the fusion of ATG16L1(+) autophagosome precursors and autophagosome formation.
Article
Cell Biology
Hannah L. Black, Rachel Livingstone, Cynthia C. Mastick, Mohammed Al Tobi, Holly Taylor, Angeline Geiser, Laura Stirrat, Dimitrios Kioumourtzoglou, John R. Petrie, James G. Boyle, Nia J. Bryant, Gwyn W. Gould
Summary: The knockout of syntaxin-4 in 3T3-L1 adipocytes was found to significantly reduce insulin-stimulated glucose transport and adiponectin secretion, but had only modest effects on the translocation capacity of the cells and the exocytic and endocytic rates of GLUT4.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Thibault Courtellemont, Maria Giovanna De Leo, Navin Gopaldass, Andreas Mayer
Summary: Endo-lysosomal compartments exchange proteins through different processes, including fusion, fission, and endosomal transport carriers. The membrane fission events that occur during these processes are not well understood. This study identifies the CROP complex as a factor that plays a role in membrane fission. The CROP complex consists of members from two protein families and enhances the membrane fission activity of a specific protein. Disrupting the CROP complex prevents fragmentation of lysosome-like structures in yeast and impairs cargo export in mammalian endosomes.
Review
Biochemistry & Molecular Biology
Amitha Muraleedharan, Benoit Vanderperre
Summary: Parkinson's disease is the second most common neurodegenerative disorder after Alzheimer's disease, and its prevalence increases with age. The endolysosomal system plays a major role in the pathogenesis of PD, and studying the genes and proteins involved in this system can lead to potential therapeutic interventions.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Navin Gopaldass, Maria Giovanna De Leo, Thibault Courtellemont, Vincent Mercier, Christin Bissig, Aurelien Roux, Andreas Mayer
Summary: Proteins exit from endosomes through retromer-coated tubular carriers, which affects cellular signaling, lysosomal biogenesis, and various diseases. The coat formation requires overcoming membrane tension. In this study, we investigated the dynamics and driving force of this process using yeast retromer, BAR-domain sorting nexins Vps5 and Vps17, and synthetic lipid tubules. The results showed that the coat forms a static tubular structure by bidirectional oligomerization, and high concentrations of sorting nexins constrict the membrane tubes. However, retromer oligomers are needed to drive constriction at lower concentrations.
Review
Biochemistry & Molecular Biology
Mai K. L. Nguyen, Jaimy Jose, Mohamed Wahba, Marc Bernaus-Esque, Andrew J. Hoy, Carlos Enrich, Carles Rentero, Thomas Grewal
Summary: Cancer cells undergo metabolic adaptions to meet their increased bioenergetic needs, including an increased demand for cholesterol. They uptake cholesterol from low-density lipoproteins (LDL) and distribute it from late endosomes/lysosomes (LE/Lys) to other organelles to promote cancer growth and spread. Understanding the regulation of cholesterol transporters and related proteins in LE/Lys is important for developing treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Femke M. Feringa, Annemiek A. van Berkel, Anushka Nair, Matthijs Verhage
Summary: Depletion of presynaptic proteins MUNC18-1 or syntaxin-1 triggers an atypical, staged cell death pathway characterized by consecutive neurite retraction, ultimately leading to, but not driven by, apoptosis.
JOURNAL OF NEUROSCIENCE
(2023)
Review
Cell Biology
Liriope Toupenet Marchesi, Marion Leblanc, Giovanni Stevanin
Summary: Hereditary spastic paraplegia (HSP) involves the degeneration of motor neurons and is characterized by clinical and genetic heterogeneity, making it challenging to find effective therapies. Several HSP genes/proteins are linked to the endolysosomal and autophagic pathways, suggesting a functional convergence, which may have implications for understanding the pathological mechanisms of HSP.
Article
Biochemistry & Molecular Biology
Elisabet Uribe-Carretero, Guadalupe Martinez-Chacon, Sokhna M. S. Yakhine-Diop, Gema Duque-Gonzalez, Mario Rodriguez-Arribas, Eva Alegre-Cortes, Marta Paredes-Barquero, Saray Canales-Cortes, Elisa Pizarro-Estrella, Antonio Cuadrado, Rosa Ana Gonzalez-Polo, Jose M. Fuentes, Mireia Niso-Santano
Summary: KEAP1 is an adaptor protein that plays an important role in regulating protein degradation. Loss of KEAP1 leads to protein oxidative aggregation and defective autophagy. Our study demonstrates that KEAP1 deficiency affects the endosome-lysosomal pathway, resulting in downregulation of cathepsin D and accumulation of autophagosomes.
Article
Cell Biology
Zoe Elizabeth West, Savannah Margaret Aitcheson, Annalese Barbara Trudy Semmler, Rachael Zoe Murray
Summary: The study demonstrates that the transportation of MT1-MMP in macrophages is regulated by the complex formed by Stx6/Stx7/Vti1b and VAMP4. These proteins form a crucial pathway through SNARE complexes to ensure efficient transport of MT1-MMP within the cell, facilitating its involvement in the migration process into injured and infected tissues.
Article
Cell Biology
Yuan Gao, Juan Xiong, Qing-Zhu Chu, Wei-Ke Ji
Summary: This study reveals that PDZD8 acts as a tether at ER-LE/lys MCSs and its SMP domain has lipid transfer activities. PDZD8 is crucial for LE/lys positioning and neurite outgrowth.
JOURNAL OF CELL SCIENCE
(2022)
Article
Immunology
Zhenfeng Wang, Jiadi Lv, Pin Yu, Yajin Qu, Yabo Zhou, Li Zhou, Qiangqiang Zhu, Shunshun Li, Jiangping Song, Wei Deng, Ran Gao, Yuying Liu, Jiangning Liu, Wei-Min Tong, Chuan Qin, Bo Huang
Summary: The study demonstrates the potential therapeutic effect of ACE2-overexpressing A549 cell-derived microparticles (AO-MPs) against SARS-CoV-2 infection by changing endosomal pH and promoting viral degradation. In addition to inhibiting the inflammatory response of alveolar macrophages, AO-MPs increase treatment efficacy in a SARS-CoV-2-infected mouse model without side effects, highlighting the potential use of AO-MPs in treating SARS-CoV-2-infected patients and the feasibility of microparticle therapies for combating emerging respiratory viruses in the future.
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)
Article
Toxicology
Kei Takahashi, Yasuhiro Morita, Shuji Udagawa, Eriko Higashi, Mayumi Nakajima, Yohei Miyamoto, Keiyu Oshida
Summary: A research conducted overseas found that a drug called DMIP induces cell death and vacuolation in human aortic vascular smooth muscle cells. The vacuolation is caused by the accumulation of DMIP in the cells, and can be significantly reduced by using a vacuolar H+- ATPase inhibitor. The study also suggests that these vacuoles are mainly enlarged late endosomes/lysosomes, and not formed through autophagy.
TOXICOLOGY IN VITRO
(2023)
Article
Cell Biology
Eva Alegre-Cortes, Alberto Gimenez-Bejarano, Elisabet Uribe-Carretero, Marta Paredes-Barquero, Andre R. A. Marques, Mafalda Lopes-da-Silva, Otilia V. Vieira, Saray Canales-Cortes, Pedro J. Camello, Guadalupe Martinez-Chacon, Ana Aiastui, Roberto Fernandez-Torron, Adolfo Lopez de Munain, Patricia Gomez-Suaga, Mireia Niso-Santano, Rosa A. Gonzalez-Polo, Jose M. Fuentes, Sokhna M. S. Yakhine-Diop
Summary: Myotonic dystrophy type 1 (DM1) is an autosomal dominant disease caused by CTG repeat expansion in the 3' untranslated region of the dystrophia myotonica protein kinase gene. Fibroblasts derived from DM1 patients show increased autophagy flux, enlarged endosomes, and lysosomes. The binding of EGF to EGFR and the internalization of EGFR are delayed in DM1 cells, but EGF-activated EGFR enhances the phosphorylation of AKT and ERK1/2.
Review
Biochemistry & Molecular Biology
Marijn Kuijpers, Domenico Azarnia Tehran, Volker Haucke, Tolga Soykan
Summary: Neurons present special challenges to the autophagy and endolysosomal systems due to their complex morphology and longevity. These systems play crucial roles in degrading defective proteins, maintaining cellular homeostasis, and participating in signaling in neurons, especially in axons.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Yue Wu, Zhenling Cui, Yen-Hua Huang, Simon J. de Veer, Andrey V. Aralov, Zhong Guo, Shayli V. Moradi, Alexandra O. Hinton, Jennifer R. Deuis, Shaodong Guo, Kai-En Chen, Brett M. Collins, Irina Vetter, Volker Herzig, Alun Jones, Matthew A. Cooper, Glenn F. King, David J. Craik, Kirill Alexandrov, Sergey Mureev
Summary: Advances in peptide and protein therapeutics have increased the demand for rapid and cost-effective polypeptide prototyping. A modified in vitro translation system allows efficient folding of disulfide-rich peptides and proteins in an aggregation-free and thermodynamically controlled environment.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Belinda L. Spillings, Christopher J. Day, Albert Garcia-Minambres, Anupriya Aggarwal, Nicholas D. Condon, Thomas Haselhorst, Damian F. J. Purcell, Stuart G. Turville, Jennifer L. Stow, Michael P. Jennings, Johnson Mak
Summary: This study reveals that HIV is captured near the lymphocyte surface in a virion-glycan-dependent manner. Removing certain glycans impairs virus-cell binding and interfering with glycan interactions reduces HIV infectivity. These findings provide new insights into the interaction between HIV and host cells.
Article
Microbiology
Thao Thanh Tran, Carmen D. Mathmann, Marcela Gatica-Andrades, Rachel F. Rollo, Melanie Oelker, Johanna K. Ljungberg, Tam T. K. Nguyen, Alina Zamoshnikova, Lalith K. Kummari, Orry J. K. Wyer, Katharine M. Irvine, Javier Melo-Bolivar, Annette Gross, Darren Brown, Jeffrey Y. W. Mak, David P. Fairlie, Karl A. Hansford, Matthew A. Cooper, Rabina Giri, Veronika Schreiber, Shannon R. Joseph, Fiona Simpson, Timothy C. Barnett, Joergen Johansson, Wendy Dankers, James Harris, Timothy J. Wells, Ronan Kapetanovic, Matthew J. Sweet, Eleanor A. Latomanski, Hayley J. Newton, Romain J. R. Guerillot, Abderrahman Hachani, Timothy P. Stinear, Sze Ying Ong, Yogeswari Chandran, Elizabeth L. Hartland, Bostjan Kobe, Jennifer L. Stow, A. Elisabeth Sauer-Eriksson, Jakob Begun, Jessica C. Kling, Antje Blumenthal
Summary: Listeria monocytogenes is a pathogen that escapes from its entry vacuole to replicate and spread in host cells. In this study, the inhibition of a key regulator of Listeria virulence resulted in the trapping of bacteria inside their entry vacuoles and extensive replication in spacious vacuoles. However, the majority of infected host immune cells were able to clear the bacteria, indicating that intra-vacuolar existence is not necessarily tied to bacterial persistence.
Article
Cell Biology
Blessy Paul, Saroja Weeratunga, Vikas A. Tillu, Hanaa Hariri, W. Mike Henne, Brett M. Collins
Summary: Recent advances in protein structure prediction using machine learning, such as AlphaFold2, have revolutionized structural biology by providing unprecedented insights into protein architecture and interactions. In this study, the researchers utilized AlphaFold2 to reveal novel structures and functions of sorting nexin proteins, particularly in regulating lipid metabolism. The findings suggest the existence of a new class of conserved lipid transfer proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
James E. B. Curson, Lin Luo, Liping Liu, Belinda J. Burgess, Nilesh J. Bokil, Adam A. Wall, Tomas Brdicka, Ronan Kapetanovic, Jennifer L. Stow, Matthew J. Sweet
Summary: A naturally occurring translational variant of Scimp (Scimp TV1) has been discovered, which is basally phosphorylated and not inducibly phosphorylated by lipopolysaccharide (LPS). Macrophages expressing Scimp TV1 showed impaired cytokine responses, while myeloid cells with high levels of Scimp and selective overexpression of Scimp TV1 exhibited enhanced cytokine production in response to CpG DNA. Furthermore, Scimp TV1 accumulated in intracellular compartments, particularly the Golgi.
IMMUNOLOGY AND CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Liping Liu, Richard M. Lucas, Jeffrey D. Nanson, Yan Li, Jason Whitfield, James E. B. Curson, Neeraj Tuladhar, Kirill Alexandrov, Mehdi Mobli, Matthew J. Sweet, Bostjan Kobe, Jennifer L. Stow, Lin Luo
Summary: In this study, we identify a novel immune-specific Syk scaffold protein SCIMP, which can contribute to inflammation through selective TLR-driven inflammatory responses.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Boris Simonetti, Qian Guo, Manuel Gimenez-Andres, Kai-En Chen, Edmund R. R. Moody, Ashley J. Evans, Mintu Chandra, Chris M. Danson, Tom A. Williams, Brett M. Collins, Peter J. Cullen
Summary: This study elucidates how SNX27-Retromer promotes endosome-to-plasma membrane recycling through its interaction with the SNX-BAR sorting complex, and reveals the stepwise evolution of this complex during early metazoan evolution.
Editorial Material
Multidisciplinary Sciences
Robert G. Parton, Brett M. Collins
Summary: The structure of caveolin, the major membrane protein of caveolae, is revealed by cryo-electron microscopy, providing new insights into its functions.
Article
Multidisciplinary Sciences
Boris Simonetti, James L. Daly, Lorena Simon-Gracia, Katja Klein, Saroja Weeratunga, Carlos Anton-Plagaro, Allan Tobi, Lorna Hodgson, Philip A. Lewis, Kate J. Heesom, Deborah K. Shoemark, Andrew D. Davidson, Brett M. Collins, Tambet Teesalu, Yohei Yamauchi, Peter J. Cullen
Summary: Endosomal sorting is important for maintaining cellular homeostasis. The study identified NRP1 as a cargo protein bound and trafficked by the ESCPE-1 complex, which may play a role in intracellular membrane trafficking of NRP1-interacting viruses such as SARS-CoV-2.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Optics
Mark L. Watson, Darren L. Brown, Alexander B. Stilgoe, Jennifer L. Stow, Halina Rubinsztein-Dunlop
Summary: Studying the mechanical properties of living cells has provided insights into the biological functions of macropinocytosis. This study used a high-resolution rotational geometry to trap and monitor a photonic probe within a macropinosome in vivo, enabling successful measurement of shear viscosity. It lays the foundation for dynamic mechanobiological studies of intracellular vesicles.
Review
Biochemistry & Molecular Biology
Richard M. Lucas, Lin Luo, Jennifer L. Stow
Summary: This article summarizes the important role of ERK1/2 in cell signaling, particularly in immune responses in immune cells. Additionally, it discusses the dysfunctional signaling of ERK1/2 in inflammatory diseases and explores the potential therapeutic benefits of targeting this pathway in inflammation.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Editorial Material
Cell Biology
Jennifer L. L. Stow, Matthew J. J. Sweet
Summary: The excessive phagocytosis of apoptotic cell corpses by macrophages in Drosophila embryos creates highly oxidative environments. A recent study by Clemente and Weavers (2023) reveals for the first time that macrophage Nrf2 is primed to sustain immune function and mitigate bystander oxidative damage.
JOURNAL OF CELL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Michael D. Healy, Brett M. Collins
Summary: PDZ and LIM domain proteins play important roles in the actin cytoskeleton and are involved in actin organization and function in metazoans. They act as scaffolds, linking various proteins to actin and its binding partner alpha-actinin. In addition to their well-known role in actin organization, emerging evidence suggests their involvement in actin-dependent membrane trafficking in the endosomal system.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Merja Joensuu, Vanessa Lanoue, Parnayan Syed, Tristan Wallis, James Rae, Ailisa Blum, Rachel Gormal, Christopher Small, Shanley Sanders, Anmin Jiang, Stefan Mahrhold, Nadja Krez, Michael Cousin, Ruby Cooper-White, Justin J. Cooper-White, Brett M. Collins, Rober G. Parton, Giuseppe Balistreri, Andreas Rummel, Frederic A. Meunier
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Seckin Akgul, Carolin Offenhauser, Anja Kordowski, Bryan W. Day
Summary: This study establishes a simple and efficient method for generating lentiviral vectors by integrating existing methods and introducing new ideas. The new method includes innovative PCR primer design, improvement of traditional methods, utilizing endogenous mRNA to construct new lentiviruses, and incorporating a purification method. The functional validity of the method is demonstrated through an infection strategy.
BIOENGINEERING-BASEL
(2022)