Review
Biochemistry & Molecular Biology
Antonio Galindo, Sean Munro
Summary: Transport Protein Particle (TRAPP) complexes are conserved complexes that act as nucleotide exchange factors for Rab GTPases. They play roles in protein secretion, autophagy, and other processes. There are two complexes in metazoans: TRAPPII activates Rab11, and TRAPPIII activates Rab1. Crystal and cryo-EM structures have provided insights into the organization and activation mechanisms of these complexes.
Review
Cell Biology
Akihiko Nakano
Summary: The structure of the Golgi apparatus varies among different species, but its functions remain conserved. In addition to the core layers of cisternae, the Golgi is accompanied by neighboring compartments on its cis and trans sides. High-resolution live imaging has revealed striking similarities in the behaviors of these compartments, indicating the presence of conserved mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Junko Y. Toshima, Ayana Tsukahara, Makoto Nagano, Takuro Tojima, Daria E. Siekhaus, Akihiko Nakano, Jiro Toshima, Sean Munro
Summary: Budding yeast possesses an independent endocytic early/sorting compartment within the trans-Golgi network (TGN) that sorts endocytic cargos. This compartment functions as the target for endocytic vesicles and is involved in recycling to the plasma membrane or transport to the Rab5 endosomal compartment. The Tlg2p protein, a homolog of yeast syntaxin, resides in this compartment and acts as a Q-SNARE for endocytic vesicles.
Article
Plant Sciences
Emi Ito, Tomohiro Uemura
Summary: This article mainly introduces the basic principles of membrane traffic and its diversity in plants. Through the study of regulators in the trans-Golgi network, it summarizes their functions in different transport pathways and their roles in plant development and environmental stimuli.
JOURNAL OF PLANT RESEARCH
(2022)
Article
Cell Biology
Viktoria Szentgyoergyi, Anne Spang
Summary: Cargo delivery in cellular organelles relies on the fusion of vesicles with the help of tethering factors. Recent studies show that tethers play a significant role in membrane fusion, and the discovery of novel tether FERARI complex has changed our understanding of cargo transport.
JOURNAL OF CELL SCIENCE
(2023)
Review
Plant Sciences
Yutaro Shimizu, Tomohiro Uemura
Summary: Membrane trafficking is crucial for organelle maintenance and function, and the TGN plays a critical role as a sorting station. Recent studies have identified distinct subdomains of the TGN for different trafficking pathways.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Aya Nishizawa, Yuto Maruta, Mitsunori Fukuda
Summary: This study investigates the differences in melanosome biogenesis and transport mechanisms between B16-F1 melanoma cells and normal melanocytes. The findings indicate the existence of a Rab32/38-independent tyrosinase transport mechanism in B16-F1 cells, highlighting a limitation in using melanoma cells as a model for studying the mechanism of endosomal tyrosinase transport in melanocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Galina Beznoussenko, Hee-Seok Kweon, Irina S. Sesorova, Alexander A. Mironov
Summary: This study compared the explanatory power of the cisterna maturation-progression model and the kiss-and-run model for the intra-Golgi transport. The authors found that their observations were more in line with the kiss-and-run model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Ann-Christin Borchers, Maren Janz, Jan-Hannes Schaefer, Arne Moeller, Daniel Kuemmel, Achim Paululat, Christian Ungermann, Lars Langemeyer
Summary: The regulation of GEF activity by Mon1 in membranes is inhibited by the N-terminal domain, resulting in alterations in endosomal structures. Mutations impairing Rab5 interaction with Mon1 lead to poor GEF activity and growth defects in endosomes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Naava Naslavsky, Steve Caplan
Summary: This Viewpoint focuses on the importance of endosomes in cells and the underlying mechanisms of their functions.
Article
Biology
Joanne Chia, Shyi-Chyi Wang, Sheena Wee, David James Gill, Felicia Tay, Srinivasaraghavan Kannan, Chandra S. Verma, Jayantha Gunaratne, Frederic A. Bard
Summary: The Src tyrosine kinase promotes the relocation of GALNTs enzymes by phosphorylating GBF1, inducing tubular transport carriers formation. This mechanism of tyrosine phosphorylation-mediated regulation may be a conserved and widespread mechanism in GEF-Arf complexes.
Article
Biochemistry & Molecular Biology
Antonio Galindo, Vicente J. Planelles-Herrero, Gianluca Degliesposti, Sean Munro
Summary: TRAPP complexes, which are nucleotide exchange factors, play vital roles in membrane traffic and autophagy. TRAPPII activates Rab11, while TRAPPIII activates Rab1, with shared core subunits affecting nucleotide exchange and specific large subunits essential for in vivo activity. Cryo-EM structures reveal the assembly of TRAPPIII complex, showing how TRAPPIII-specific subunits hold the catalytic core and large arms determine specificity. The lower resolution structure of TRAPPII suggests evolution from a single ur-TRAPP.
Review
Biochemistry & Molecular Biology
Qingchun Lu, Po-Shun Wang, Ling Yang
Summary: This review focuses on highlighting Golgi-associated Rab GTPases as master regulators of autophagic flux, discussing the future research prospects in elucidating their role and mechanism in autophagy and autophagy-related diseases.
CELL AND BIOSCIENCE
(2021)
Article
Chemistry, Analytical
Zi Gao, Yen-Yu Yang, Ming Huang, Tianyu F. Qi, Handing Wang, Yinsheng Wang
Summary: Radiation therapy is beneficial for cancer patients, and this study provides a comprehensive investigation of the differential expression of small GTPase proteins associated with the development of radioresistance in breast cancer cells. The study identifies ARFRP1 as a novel regulator of radioresistance and a potential target for enhancing radiation sensitivity in breast cancer. This research has important implications for the treatment of breast cancer.
ANALYTICAL CHEMISTRY
(2022)
Article
Biology
R. Sikora, P. Bun, L. Danglot, M. Alqabandi, P. Bassereau, F. Niedergang, T. Galli, A. Zahraoui
Summary: Secreted proteins are transported along intracellular route from the endoplasmic reticulum through the Golgi before reaching the plasma membrane. Small GTPase Rab and their effectors play a key role in membrane trafficking. MICAL-L1 is found to be associated with tubulo-vesicular structures and to have a significant colocalization with markers of the Golgi apparatus and recycling endosomes, revealing a new role in cargo delivery to the plasma membrane.
Article
Cell Biology
Nikit Kumar, Marianna Leonzino, William Hancock-Cerutti, Florian A. Horenkamp, Peiqi Li, Joshua A. Lees, Heather Wheeler, Karin M. Reinisch, Pietro De Camilli
JOURNAL OF CELL BIOLOGY
(2018)
Article
Cell Biology
Diana P. Valverde, Shenliang Yu, Venkata Boggavarapu, Nikit Kumar, Joshua A. Lees, Thomas Walz, Karin M. Reinisch, Thomas J. Melia
JOURNAL OF CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Yixian Cui, Smriti Parashar, Muhammad Zahoor, Patrick G. Needham, Muriel Mari, Ming Zhu, Shuliang Chen, Hsuan-Chung Ho, Fulvio Reggiori, Hesso Farhan, Jeffrey L. Brodsky, Susan Ferro-Novick
Article
Cell Biology
PeiQi Li, Joshua Aaron Lees, C. Patrick Lusk, Karin M. Reinisch
JOURNAL OF CELL BIOLOGY
(2020)
Review
Cell Biology
Joshua A. Lees, Karin M. Reinisch
CURRENT OPINION IN CELL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Shuliang Chen, Muriel Mari, Smriti Parashar, Dongmei Liu, Yixian Cui, Fulvio Reggiori, Peter J. Novick, Susan Ferro-Novick
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Joshua A. Lees, PeiQi Li, Nikit Kumar, Lois S. Weisman, Karin M. Reinisch
Review
Cell Biology
Karin M. Reinisch, William A. Prinz
Summary: Lipids can be transported between cellular membranes via nonvesicular mechanisms, supporting biogenesis of rapidly expanding membranes or allowing targeted modulation of specific lipid levels. Nonvesicular transport likely occurs at membrane contact sites and involves lipid transport proteins, but many questions remain unanswered regarding the mechanisms involved.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Dong Huang, Bolin Xu, Lu Liu, Lingzhi Wu, Yuangang Zhu, Alireza Ghanbarpour, Yawei Wang, Feng-Jung Chen, Jia Lyu, Yating Hu, Yunlu Kang, Wenjing Zhou, Xiao Wang, Wanqiu Ding, Xin Li, Zhaodi Jiang, Jizheng Chen, Xu Zhang, Hongwen Zhou, John Zhong Li, Chunguang Guo, Wen Zheng, Xiuqin Zhang, Peng Li, Thomas Melia, Karin Reinisch, Xiao-Wei Chen
Summary: This study revealed the role of TMEM41B in phospholipid shuttling process, highlighting its critical importance in maintaining ER function and lipid homeostasis.
Article
Multidisciplinary Sciences
Alireza Ghanbarpour, Diana P. Valverde, Thomas J. Melia, Karin M. Reinisch
Summary: ATG2 interacts with TMEM41B, VMP1, and ATG9 in Golgi-derived vesicles to initiate autophagosome formation, with TMEM41B and VMP1 playing key roles in lipid homeostasis and membrane dynamics processes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Marianna Leonzino, Karin M. Reinisch, Pietro De Camilli
Summary: Recent studies have discovered a novel protein-mediated mechanism of lipid transport, where a rod-like protein bridge with a hydrophobic groove facilitates the transfer of lipids between organelles in eukaryotic cells. This mechanism is well-suited for bulk transport of bilayer lipids to promote membrane growth.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Cell Biology
Jyoti Adlakha, Zhouping Hong, PeiQi Li, Karin M. Reinisch
Summary: VPS13 proteins act as bridges between organelles, facilitating directional and bulk lipid transport. They are anchored between membranes through interactions with receptors. The study reveals that Mcp1p and XK, two integral membrane proteins that interact with VPS13A, are scramblases.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Michael G. Hanna, Patreece H. Suen, Yumei Wu, Karin M. Reinisch, Pietro De Camilli
Summary: Cellular membranes differ in protein and lipid composition, and the transfer of lipids between membranes may play a role in their trafficking. Recent studies on the VPS13 family proteins and SHIP164 suggest that lipid transfer between membranes can occur independently of vesicular traffic.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Thomas J. Melia, Karin M. Reinisch
Summary: This article reviews the structure and function of a new class of lipid transporters, discussing their coordination with other lipid transport proteins. It also explores the potential role of this type of lipid transport in membrane expansion and other emerging hypotheses and questions.
JOURNAL OF CELL SCIENCE
(2022)
Article
Cell Biology
Zhouping Hong, Jyoti Adlakha, Neng Wan, Emily Guinn, Fabian Giska, Kallol Gupta, Thomas J. Melia, Karin M. Reinisch
Summary: Mitoguardin-2 is a mitochondrial protein that binds fatty acids and phospholipids at contacts with the ER or lipid droplets, and can transfer phospholipids between membranes. It plays crucial roles in mitochondrial and lipid droplet biology.
JOURNAL OF CELL BIOLOGY
(2022)