Article
Fisheries
Ying Huang, Rui-Xia Wang, Fu-Hui Jiang, Xiao-Tong Xu, Yan Shi, Zhe Zhao
Summary: ToCnx is a novel Cnx homologue from Takifugu obscurus, possessing typical characteristics of fish Cnxs and having the closest relationship with Cnxs from Takifugu flavidus and Takifugu rubripes. It is mainly expressed in the liver, kidney, and intestine, with significant upregulation in the kidneys upon bacterial infection. The recombinant calreticulin domain of ToCnx can bind and agglutinate various bacteria in the absence and presence of calcium, suggesting its role in host immune defense.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Environmental Sciences
Alejandro Reyes-Cervantes, Diana Laura Robles-Morales, Alejandro Tellez-Jurado, Sergio Huerta-Ochoa, Angelica Jimenez-Gonzalez, Sergio Alejandro Medina-Moreno
Summary: The study evaluated and characterized the biodegradation of the herbicide diuron in its commercial form above saturation concentration by acclimatized Lysinibacillus fusiformis. The results showed improved capacity of L. fusiformis to remove diuron at high concentrations, indicating potential for bioremediation of diuron-contaminated sites.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Environmental Sciences
Jun Wang, Xuexia Zhu, Yunfei Sun, Lei Gu, Yaping Wu, Yafen Chen, Zhou Yang
Summary: This study investigated the molecular and physiological changes underlying salinity tolerance in the obscure puffer fish using transcriptomics and micrography. The study found that as salinity increased, the structure of the gill, intestine, and kidney, which are the main osmoregulatory organs, underwent significant changes. Transcriptome analysis revealed reduction in ATP-binding box transporter and cyclic adenosine monophosphate (cAMP) signaling pathway in the gill and kidney. Growth hormone, prolactin, and cortisol were found to regulate transporters, chloride cell proliferation, and differentiation. Adenosine-activating protein kinase and thyroid hormone signaling pathway were also upregulated. These findings indicate that osmoregulation in fish migration is a complex process involving multiple organs and signaling pathways.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Shi-Weng Li, Ya Wen, Yan Leng
Summary: The study revealed that the Cr(VI)-reducing Lysinibacillus fusiformis 15-4 strain employs a variety of genes and pathways in the reduction of Cr(VI), including oxidoreductases, dehydrogenases, cell redox homeostasis proteins, and stress response proteins which were significantly upregulated. On the other hand, Na+:H+ antiporter complex-coding genes and transcriptional regulator-coding genes were notably downregulated in response to Cr treatment. Multiple FMN/NAD(P)H-dependent reductase-encoding genes were induced by Cr, suggesting their involvement in Cr(VI) reduction in strain 15-4. Synergistic roles of sulfur, iron ions, and thiol-disulfide exchange reactions were also implicated in Cr reduction processes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ying Huang, Yun-Feng Li, Rui-Xia Wang, Meng-Fan Xie, Yan Shi, Zhe Zhao
Summary: A Crt homolog named ToCrt was identified in the obscure puffer Takifugu obscurus, showing antimicrobial activity and playing a role in immune response. ToCrt was widely expressed in various tissues and significantly upregulated after stimulation to combat bacteria.
MOLECULAR IMMUNOLOGY
(2021)
Review
Infectious Diseases
Hiroshi Morioka, Keisuke Oka, Yosuke Yamada, Yoshimasa Nakane, Hitoshi Komiya, Chiaki Murase, Mitsutaka Iguchi, Tetsuya Yagi
Summary: This article reports a case of a 93-year-old woman diagnosed with Lysinibacillus fusiformis bacteremia complicated with coma blisters. Through multiple stainings and mass spectrometry identification, the organism was definitively identified as L. fusiformis. The patient showed improvement with the two-week administration of antibiotics.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2022)
Article
Fisheries
Ying Huang, Xin-Yue Yu, Peng Luo, Fu-Hui Jiang, Li-Fan Cui, Yan Shi, Xiao-Rui Song, Zhe Zhao
Summary: In this study, three L-type lectin homologs from the obscure puffer Takifugu obscurus were cloned and identified for the first time. These lectins were found to be widely expressed in different tissues, with relatively high levels in the liver and intestine. Their expression levels were significantly altered after bacterial induction. Recombinant proteins of these lectins showed calcium-dependent agglutination with various bacteria and inhibited the growth of certain bacteria by binding to specific molecules.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Fan-Xiang Gao, Wei-Jia Lu, Yan Shi, Li Zhou, Jian-Fang Gui, Zhe Zhao
Summary: IRF7, a key regulator in the type I interferon response, has been found to have three alternative splicing variants in obscure puffer fish. These variants show differences in their abilities to induce transcription of interferon and interferon-stimulated genes, suggesting potential distinct roles in immune response regulation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Fan-Xiang Gao, Wei-Jia Lu, Yan Shi, Zhan-Wei Zhao, Li Zhou, Jian-Fang Gui, Zhe Zhao
Summary: This study systematically analyzed IFN genes in pufferfish and identified different types of IFN genes, studying their molecular characteristics and phylogenetic relationships. The results showed that these IFN genes have different expression patterns in various tissues of pufferfish and play multiple roles in antiviral responses. Additionally, specific transcription factor binding sites and induction effects were investigated.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Rui-Xia Wang, Ying Huang, Yan Shi, Fu-Hui Jiang, Yang Gao, Xin Liu, Zhe Zhao
Summary: This study cloned and characterized a c-type lysozyme gene ToLyzC from the obscure puffer, and found that it plays an important role in the immune response against bacterial infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Microbiology
Brittny R. Smith, Kistie B. Patch, Anjali Gupta, Emma M. Knoles, Robert L. Unckless
Summary: The genetic causes of phenotypic variation differ among populations, and may also differ for similar traits. Differences in genetic causes among populations suggest context dependence, while similarities suggest pleiotropy. We found no overlap in the genetic architecture of variation in survival post infection of a bacterial pathogen, but a shared QTL peak was found when comparing to a fungal pathogen. This suggests that different populations adapt differently, but similar genetic variation occurs within a population.
Article
Multidisciplinary Sciences
Suliat Olatidayo Omisore, Temitope Bukola Fabunmi, Adeyemi Oluwadare Ayodeji, Oladipo Oladiti Olaniyi, Daniel Juwon Arotupin
Summary: Xylanases are important enzymes for the conversion of xylan-rich lignocellulosic waste. This study presents the physicochemical and kinetic properties of a purified endoxylanase produced by Lysinibacillus fusiformis. The purified enzyme showed high activity at 60 degrees C and pH 5.0, and had good stability. It exhibited hydrolytic activity on Birchwood xylan and kolanut husk, but low or no activity on carboxymethyl cellulose, starch or pectin.
Article
Green & Sustainable Science & Technology
Ines Taieb, Sonia Ben Younes, Boutheina Messai, Sami Mnif, Ridha Mzoughi, Amina Bakhrouf, Chedia Jabeur, Juan Alfonso Ayala Serrano, Ali Ellafi
Summary: The study successfully isolated a strain of Lysinibacillus fusiformis from Metlaoui phosphate laundries wastewater, which demonstrated survival under high metal ion concentrations and resistance to metal stress. Through characterization in terms of enzyme production, proteomic alteration, and antibiotic resistance, this strain showed promising bioprocessing performance in treating MPLW.
Article
Environmental Sciences
Kexin Song, Wengang Du, Xiaona Ma, Yangjun Chen, Yixin Sun, Tao Zhang, Wei Huang, Zhihua Feng
Summary: Microplastics in fish pose risks to ecology and food safety. This study investigated the characteristics of microplastics in fugu and found that over 98.85% of fugu were contaminated by microplastics. Wild fugu had a lower abundance of microplastics compared to cultured fugu, and the abundance of microplastics varied among different tissues and life patterns.
MARINE POLLUTION BULLETIN
(2022)
Article
Microbiology
Alessandro Passera, Marzia Rossato, John S. Oliver, Giovanna Battelli, Gul-I-Rayna Shahzad, Emanuela Cosentino, Jay M. Sage, Silvia L. Toffolatti, Giulia Lopatriello, Jennifer R. Davis, Michael D. Kaiser, Massimo Delledonne, Paola Casati
Summary: This study characterized Lysinibacillus fusiformis strain S4C11 isolated from the roots of an apple tree in northern Italy using various approaches such as in vitro, in vivo, in planta, and in silico methods. The strain exhibited antifungal activity, plant growth promotion abilities, and its genome analysis revealed genes related to biocontrol effects observed in experimental trials.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Mohammadhossein Movassaghghazani, Nazanin Shabansalmani
Summary: The study revealed high levels of AFM1 in both breast milk and powdered milk samples in Tehran, with some samples exceeding the established limits. Therefore, monitoring and regulating toxin levels in these products is crucial for maternal and infant health.
Article
Pharmacology & Pharmacy
Defeng Wen, Wantong Han, Quan Chen, Guanhui Qi, Mengling Gao, Pu Guo, Yu Liu, Zhongyuan Wu, Shulin Fu, Qirong Lu, Yinsheng Qiu
Summary: Luteolin can inhibit FB1-induced intestinal inflammatory injury by inhibiting the activation of NF-kappa B and ERK signaling pathways, according to network pharmacology and in vitro experiments.
