Article
Biochemistry & Molecular Biology
Arina G. Kicheeva, Ekaterina S. Sushko, Lyubov S. Bondarenko, Kamila A. Kydralieva, Denis A. Pankratov, Nataliya S. Tropskaya, Artur A. Dzeranov, Gulzhian I. Dzhardimalieva, Mauro Zarrelli, Nadezhda S. Kudryasheva
Summary: The current study investigates the role of reactive oxygen species (ROS) in the bioeffects of different types of magnetite nanoparticles (MNPs) - bare (Fe3O4), humic acids (Fe3O4-Ha), and 3-aminopropyltriethoxysilane (Fe3O4-APTES) modified MNPs. The study found that Fe3O4-HA MNPs had the lowest amount of Fe2+ ions, while Fe3O4-APTES MNPs had the highest amount of Fe2+ ions. Bioluminescent cellular and enzymatic assays were used to monitor the toxicity and antioxidant activity of the MNPs. The results showed that the modified MNPs had toxic effects at higher concentrations (>10(-2) g/L) and stimulated ROS production by bacteria in a wide concentration range (10(-15)-1 g/L).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Renata Biba, Petra Cvjetko, Mihaela Jakopcic, Bruno Komazec, Mirta Tkalec, Nino Dimitrov, Tajana Begovic, Biljana Balen
Summary: Plastic contamination is a pressing environmental issue due to the increasing production of disposable plastic products. In this study, onion roots were exposed to commercial polystyrene (PS-MPs) and polymethyl methacrylate microparticles (PMMA-MPs) to investigate their effects. The results showed that PS-MPs were more readily taken up by onion roots compared to PMMA-MPs. Both types of microparticles did not induce toxic effects on root growth, and PMMA-MPs even stimulated root growth. Antioxidant enzymes were activated in root cells after exposure to both types of microparticles.
Review
Plant Sciences
Petr Dvorak, Yuliya Krasylenko, Adam Zeiner, Jozef Samaj, Tomas Takac
Summary: Reactive oxygen species (ROS) are essential signaling molecules in plant responses to environmental stimuli and development. Antioxidant enzymes play a critical role in scavenging ROS and their regulation is influenced by both environmental conditions and transcriptional activation.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Chemistry, Multidisciplinary
Zhao Jinyuan, Zhang Qian, Wang Jian, Zhang Qi, Li Heng, Du Yaping
Summary: This paper briefly reviews the research progress on scavenging materials for ROS and proposes a three-level pyramidic structure for the design of related materials. The design of nano-hybrid free radical scavenger not only solves the toxicity problem, but also provides additional functions.
ACTA CHIMICA SINICA
(2022)
Article
Biochemistry & Molecular Biology
Iwona Lukasik, Sylwia Golawska, Hubert Sytykiewicz
Summary: Changes in oxidative stress markers and antioxidant enzyme activities were studied in the black bean aphid. Winged adults showed lower oxidative stress marker levels and higher antioxidant enzyme activities, indicating adaptation to new host plants. Wingless females during spring migration showed increased marker levels and enzyme activities, suggesting biochemical adaptation to winter hosts.
Article
Chemistry, Physical
Hassan O. Shaikhaldein, Fahad Al-Qurainy, Mohammad Nadeem, Salim Khan, Mohamed Tarroum, Abdalrhaman M. Salih, Saleh Alansi, Abdulrahman Al-Hashimi, Alanoud Alfagham, Jawaher Alkahtani
Summary: This study examines the effects of biosynthesized silver nanoparticles on salt-stressed Maerua oblongifolia plants and finds that these nanoparticles can alleviate the adverse effects of salt stress and improve plant growth and antioxidant defense systems.
Article
Biochemistry & Molecular Biology
Noor Akmal Shareela Ismail, Jun Xin Lee, Fatimah Yusof
Summary: This study found that platinum nanoparticles possess high antioxidant capacity and can reduce oxidative stress in lung cancer cells. Additionally, platinum nanoparticles can decrease DNA damage and inhibit ROS production, as well as increase antioxidant enzyme activities. Therefore, platinum nanoparticles could be a promising antioxidant in the treatment of lung cancer.
Review
Biochemistry & Molecular Biology
Yang Zhang, Mengqiu Hao, Xuyang Yang, Su Zhang, Junhong Han, Ziqiang Wang, Hai-Ning Chen
Summary: Colorectal cancer often requires adjuvant therapies to reduce tumor burden, and the efficacy of these therapies is significantly influenced by reactive oxygen species (ROS). ROS-mediated colorectal cancer adjuvant therapies involve multiple mechanisms, and preliminary clinical trials have shown the potential of ROS-manipulating therapy in enhancing treatment outcomes.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Review
Plant Sciences
Anna Ekner-Grzyb, Anna Duka, Tomasz Grzyb, Isabel Lopes, Jagna Chmielowska-Bak
Summary: Pollution of the environment with plastic is a significant concern in the modern world. Despite recycling methods, a substantial amount of plastic is still deposited in the environment. Nanoplastic, in particular, poses a serious danger as it can be effectively taken up and transported within organisms. It has been found to exert toxic effects on plants, mainly through oxidative response.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yuanduo Wang, Piaopiao Tan, Liang Chang, Zheming Yue, Chaozhen Zeng, Mei Li, Zhixiang Liu, Xujie Dong, Mingli Yan
Summary: Exogenous proline treatment enhances the tolerance of Brassica juncea to cadmium by reducing cadmium accumulation and reestablishing redox homeostasis.
Article
Microbiology
Anjney Sharma, Xiu-Peng Song, Rajesh Kumar Singh, Anukool Vaishnav, Saurabh Gupta, Pratiksha Singh, Dao-Jun Guo, Krishan K. Verma, Yang-Rui Li
Summary: The overconsumption and irregular use of agrochemicals can have adverse effects on soil flora and non-target beneficial microorganisms. This study focused on the effects of a high dosage of fungicide on the survival of plant-beneficial rhizobacteria and their ability to promote plant growth. The results showed that the high dosage of fungicide decreased the cell growth and survival of the rhizobacteria and also reduced their plant growth promoting activities.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biology
Peiman Zandi, Ewald Schnug
Summary: Environmental changes caused by anthropogenic activities have a significant impact on plants, leading to the generation of reactive oxygen species (ROS) and ultimately oxidative stress. Plants have developed defensive mechanisms, including enzymatic and non-enzymatic antioxidants, to scavenge excess ROS and maintain cellular redox homeostasis. Additionally, plant-microbe interactions have been found to improve immune systems in plants under stress. Understanding and studying these antioxidant systems and beneficial microbiome interactions in plants are important for plant survival and productivity.
Article
Biotechnology & Applied Microbiology
Jiwang Deng, Bangxian Yu, Zhenglin Chang, Sicheng Wu, Guanlin Li, Wenzhe Chen, Shujue Li, Xiaolu Duan, Wenqi Wu, Xinyuan Sun, Guohua Zeng, Hongxing Liu
Summary: Oxidative stress damage to renal epithelial cells plays a significant role in the formation of calcium oxalate calculi. In this study, porous nanorods CeO2 nanoparticles (CNPs) were used as an antioxidant reagent to suppress kidney calcium oxalate crystal depositions. The results showed that CNPs could effectively reduce oxidative damage, inhibit calcium oxalate crystallization, and protect renal epithelial cells from stress damage.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Plant Sciences
Samrah Afzal Awan, Imran Khan, Qi Wang, Jing Gao, Xianming Tan, Feng Yang
Summary: The hormone melatonin can enhance the germination potential and other physiological attributes of soybean seeds under abiotic stresses. In particular, at concentrations of 50 mu mol L-1 and 100 mu mol L-1, melatonin significantly improved the germination indices and other physiological attributes of soybean seeds.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Chen Dong, Xuehua Ma, Yi Huang, Yujie Zhang, Xiang Gao
Summary: This study demonstrates the synthesis of carbon dots nanozyme with excellent scavenging activity of reactive oxygen species (ROS) for anti-inflammatory therapy, indicating its potential in the treatment of inflammation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Andrea Soltysova, Patricia Begerova, Kristina Jakic, Katarina Kozics, Monika Sramkova, Eckart Meese, Bozena Smolkova, Alena Gabelova
Summary: The unique physicochemical properties of inorganic nanoparticles make them an exciting tool in diagnosis and disease management. However, their difficulty in degradation and excretion may lead to unintended accumulation in tissues and adverse health effects. This study provides a methylome-transcriptome framework for assessing the chronic effects of commonly used inorganic nanoparticles in human kidney cells, revealing deregulated genes related to immune response, DNA damage, and cancer pathways.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Meeting Abstract
Oncology
Michal Chovanec, Katarina Kalavska, Jana Obertova, Patrik Palacka, Katarina Rejlekova, Zuzana Sycova-Mila, Valentina De Angelis, Zuzana Orszaghova, Peter Lesko, Daniela Svetlovska, Beata Mladosievicova, Jozef Mardiak, Michal Pastorek, Barbora Vlkova, Peter Celec, Michal Mego
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lucia Balintova, Miroslava Matuskova, Alena Gabelova
Summary: 5-Fluorouracil is an essential chemotherapeutic drug for colorectal cancer treatment, but drug resistance is a common issue. The study explored the potential of the epigenetic drug SAHA to increase sensitivity of chemoresistant CRC cells to 5-FU, with a focus on genotoxic risk. The combination treatment showed increased sensitivity in some cell lines but potential genotoxic effects.
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2022)
Article
Chemistry, Multidisciplinary
Tibor Dubaj, Katarina Kozics, Monika Sramkova, Alena Manova, Neus G. Bastus, Oscar H. Moriones, Yvonne Kohl, Maria Dusinska, Elise Runden-Pran, Victor Puntes, Andrew Nelson, Alena Gabelova, Peter Simon
Summary: Data for assembling a physiologically-based pharmacokinetic (PBPK) model for nanoparticles (NPs) are relatively scarce, leading to a trend of extrapolating results from in vitro and in silico studies to in vivo nanoparticle hazard and risk assessment. To evaluate the reliability of this approach, a pharmacokinetic study was conducted using the same polyethylene glycol-coated gold nanoparticles (PEG-AuNPs) in vitro and in vivo. The study found notable differences in the internalized amount of Au between individual cell lines and corresponding tissues in vivo, indicating the caution needed when extrapolating in vitro data to predict the in vivo NP burden and response to exposure.
Article
Engineering, Chemical
Michal Pastorek, Maria Suchonova, Barbora Konecna, Slavomir Pasztor, Jakub Petrus, Nadja Ivaskova, Peter Celec, Roman Gardlik, Zdenko Machala, L'ubomira Tothova
Summary: Urinary tract infections (UTI) caused by resistant uropathogenic bacteria are a serious health concern. This study aimed to evaluate the potential of cold plasma activated liquid (PAL) as a treatment for UTI using an animal model. However, the in vivo experiments showed that PAL had no significant effect on bacterial load in the mouse model of UTI, possibly due to the attenuating effect of urine and its toxicity on immune cells.
PLASMA CHEMISTRY AND PLASMA PROCESSING
(2022)
Review
Immunology
Michal Pastorek, Martin Dubrava, Peter Celec
Summary: Despite ongoing vaccination, COVID-19 remains a global healthcare problem due to the lack of effective targeted therapy. Severe cases of COVID-19 involve uncontrolled activation of the innate immune system, resulting in cytokine deregulation, release of damage-associated molecular patterns, and a high occurrence of thrombotic events. These pathomechanisms are associated with neutrophil function and dysfunction, specifically the excessive formation of neutrophil extracellular traps (NETs). This review aims to summarize potential inducers of NETs formation in severe COVID-19 and discuss potential treatment options targeting NETs formation or removal.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Michal Pastorek, Diana Drobna, Peter Celec
Summary: The etiology of autism spectrum disorders (ASD) is complex and involves genetic, hormonal, and environmental factors. The interactions between increased testosterone, certain genetic mutations, and prenatal immunological stress are proposed to drive the development of ASD. The formation of neutrophil extracellular traps (NETs), which are pro-inflammatory and can affect blood-brain barrier permeability, may be an initiating factor in ASD development.
MEDICAL HYPOTHESES
(2022)
Review
Psychiatry
Miriam Pillerova, Veronika Borbelyova, Michal Pastorek, Vladimir Riljak, Julius Hodosy, Karyn M. Frick, L'ubomira Tothova
Summary: Anxiety disorders are common mood disorders that can significantly impact quality of life. Dysregulated sex steroid hormones during early brain development may increase the risk of anxiety disorders in adulthood, with evidence of sex-specific effects. Understanding the molecular mechanisms involved may provide insights for potential therapeutic targets in the future.
FRONTIERS IN PSYCHIATRY
(2022)
Article
Multidisciplinary Sciences
Lucia Mihalovicova, Veronika Kunstekova, David Milacek, Jakub Janko, Michal Pastorek, Barbora Konecna, Radana Gurecka, Zuzana Rausova, Olga Ulicna, Peter Celec, Katarina Sebekova
Summary: The study investigates the impact of maternal gestational diabetes (GDM) on the metabolic status of offspring. GDM was induced in CD1 mice consuming a fast-food diet (FFD) before mating. Offspring from dams consuming standard chow or FFD served as controls. Results showed that male GDM offspring exhibited growth retardation and lower kidney weight. GDM offspring, regardless of sex, had the lowest IL-1 alpha levels, while other inflammatory markers showed inconsistent changes. Offspring from dams consuming FFD had higher liver triacylglycerols content.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Barbora Svitkova, Michal Selc, Veronika Nemethova, Filip Razga, Alena Gabelova, Monika Ursinyova, Andrea Babelova
Summary: Fundamental studies investigating the biological effects induced by nanoparticles require accurate assessment of their intracellular concentration. Ultrasensitive atomic absorption spectroscopy is seen as a gold standard method, but comes with limitations and complex sample preparation. In this study, plate reader spectroscopy is proposed as a reliable and affordable alternative, providing comparable results to atomic absorption spectroscopy for evaluating intracellular nanoparticle concentration.
Article
Biochemistry & Molecular Biology
Kristina Licha, Michal Pastorek, Gabriela Repiska, Peter Celec, Barbora Konecna
Summary: The study aimed to investigate the presence of ecDNA in sEVs isolated from fresh human plasma by ultracentrifugation and density gradient. It was found that approximately 60-75% of DNA is on the surface of sEVs, while a part of the DNA is localized inside the sEVs. Furthermore, both nuclear and mitochondrial DNA were present in plasma EVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Biophysics
P. Svec, M. Fussiova, M. Janikova, M. Pastorek, J. Horakova, I. Bodova, A. Panikova, J. Adamcakova, T. Sykora, D. Doczyova, V. Dobsinska, M. Pozdechova, B. Vlkova, A. Kolenova, P. Celec
BONE MARROW TRANSPLANTATION
(2022)
Meeting Abstract
Physiology
B. M. Schuh, V. Borbelyova, K. Macakova, T. Gross, P. Belvoncikova, A. Fejes, J. Janko, M. Pastorek, B. Vlkova, P. Celec
Meeting Abstract
Physiology
V. Borbelyova, A. Fejes, I. Slivkova, K. Macakova, A. Hladikova, E. Becka, M. Pastorek, K. Sebekova, P. Celec