Article
Multidisciplinary Sciences
Linda Milne, Micha Bayer, Paulo Rapazote-Flores, Claus-Dieter Mayer, Robbie Waugh, Craig G. Simpson
Summary: A high-quality barley gene reference transcript dataset (BaRTv1.0) was used to quantify gene and transcript abundances across 22 RNA-seq experiments, leading to the development of a Barley Expression Database (EORNA) for visualizing comparative gene and transcript abundance data. The database provides gene and transcript models for all transcripts in BaRTv1.0, allowing users to easily identify and analyze transcript data.
Article
Biology
Richard J. White, Eirinn Mackay, Stephen W. Wilson, Elisabeth M. Busch-Nentwich
Summary: In zebrafish, differentially expressed genes are often over-represented on the same chromosome as the mutation, due to different levels of expression of alleles from different genetic backgrounds. This has implications for interpreting the results and pathways in RNA-seq experiments.
Article
Agriculture, Dairy & Animal Science
Carla Moros-Nicolas, Ma Jose Izquierdo-Rico, Yang Li, Leopoldo Gonzalez-Brusi, Raquel Romar, Hiroaki Funahashi
Summary: This study examined the relative transcript abundance of cumulus cells (CCs) derived from different sized follicles and found significant differences in the expression of five genes, providing new markers for identifying viable and competent oocytes.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Reproductive Biology
Jian-fei Wu, Yu Liu, San-ni Gong, Xiang-dong Zi, You-guo Tan
Summary: This study investigated the effects of VEGF on the viability, apoptosis, and steroidogenesis of yak granulosa cells. The results showed that VEGF can promote cell viability, decrease apoptosis, and increase hormone synthesis in granulosa cells. These findings highlight the beneficial influence exerted by VEGF in improving granulosa cell viability and reducing apoptosis.
Article
Multidisciplinary Sciences
Yi-Fan Jiang, Pin-Huan Yu, Yovita Permata Budi, Chih-Hsien Chiu, Chi-Yu Fu
Summary: This study investigated the dynamics of mitochondria in mammalian ovaries during folliculogenesis and luteal formation. The results showed that mitochondrial networks shifted from branched structures in follicles to spherical and tubular mitochondria in large luteal cells. Additionally, mitochondrial diameter and volume increased from folliculogenesis to luteal formation, indicating mitochondrial enlargement as an event during these processes. Modulation of mitochondrial morphology and respiratory enzyme expressions may be related to tissue remodeling during luteal formation.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Qiang Liu, Hui Gao, Feng Yang, Hanxue Zhang, Shenming Zeng
Summary: This study revealed that FSH stimulates progesterone production in mammalian follicular granulosa cells through a non-canonical pathway, while also promoting the process of autophagy. Moreover, FSH was found to facilitate the degradation of lipid droplets, and inhibiting autophagy led to decreased progesterone production.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Liang Huang, Lutong Zhang, Shengjie Shi, Xiaoge Zhou, Huan Yuan, Xiangrong Song, Yamei Hu, Weijun Pang, Gongshe Yang, Lei Gao, Guiyan Chu
Summary: The CLOCK gene plays a critical role in estradiol synthesis in porcine granulosa cells. The alteration of the CLOCK gene leads to impaired mitochondrial function and reduced estradiol synthesis.
Article
Biochemistry & Molecular Biology
Freed Ahmad, Paul Debes, Ilkka Nousiainen, Siim Kahar, Lilian Pukk, Riho Gross, Mikhail Ozerov, Anti Vasemagi
Summary: The study introduces a new analytical framework for quantifying ongoing natural selection at the transcriptome level in wild vertebrates. By integrating mark-recapture field sampling and RNA-sequencing with regression-based selection analysis, the study estimated selection on transcript abundance in wild salmonid fish affected by an extracellular parasite. The results reveal upregulation of mitotic cell cycle process in infected hosts and disruptive selection signals on transcripts related to host immune defense, host-pathogen interactions, cellular repair, and maintenance.
Review
Biotechnology & Applied Microbiology
Esperanza Rivera-de-Torre, Charlotte Rimbault, Timothy P. Jenkins, Christoffer V. Sorensen, Anna Damsbo, Natalie J. Saez, Yoan Duhoo, Celeste Menuet Hackney, Lars Ellgaard, Andreas H. Laustsen
Summary: Animal venoms contain toxins that play a crucial role in understanding toxicity mechanisms and developing biotechnological applications. Heterologous expression of toxins is an important approach to produce a sufficient amount of bioactive proteins. It ensures high purity, avoids contamination, and enables the design of toxins with specific properties.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Leyan Yan, Mengdie Hu, Lihong Gu, Mingming Lei, Zhe Chen, Huanxi Zhu, Rong Chen
Summary: This study aimed to elucidate the molecular mechanisms underlying abnormal egg-laying in laying hens subjected to heat stress. The results showed that heat stress impaired laying performance in hens, causing damage to follicular granulosa cells and dysfunction in steroidogenesis.
Article
Reproductive Biology
Dongmei Li, Xinyan Li, Haorong He, Yao Zhang, Hua He, Congjiao Sun, Xinyi Zhang, Xunzi Wang, Zhaoyi Kan, Yang Su, Shunshun Han, Lu Xia, Bo Tan, Mengen Ma, Qing Zhu, Huadong Yin, Can Cui
Summary: This study reveals a novel role for miR-10a-5p in inhibiting the proliferation and progesterone synthesis in chicken ovarian granulosa cells. The target gene of miR-10a-5p is identified as MAPRE1, which is involved in promoting progesterone synthesis and cell proliferation.
Article
Agriculture, Dairy & Animal Science
Magdalena Szymanska, Agnieszka Blitek
Summary: The study examined the impact of impaired progesterone synthesis on uterine receptor expression in pigs. It was found that impaired progesterone synthesis led to decreased luteal progesterone levels during pregnancy and affected receptor expression in both the endometrium and myometrium, with the endometrium being more sensitive to impaired progesterone synthesis.
ANNALS OF ANIMAL SCIENCE
(2023)
Article
Genetics & Heredity
Lewei Guo, Haoran Xu, Yajun Li, Hongyu Liu, Jing Zhao, Wenfa Lu, Jun Wang
Summary: This study aimed to investigate the impact of kp-10 on P4 synthesis in BGCs and its underlying mechanisms involving miR-1246. The results demonstrated that kp-10 promoted P4 synthesis in BGCs by regulating miR-1246/StAR expression.
Article
Biochemistry & Molecular Biology
Khoi Thieu Ho, Ahmed Zaky Balboula, Kohei Homma, Jun Takanari, Hanako Bai, Manabu Kawahara, Khang Thi Kim Nguyen, Masashi Takahashi
Summary: The standardized extract of Asparagus officinalis stem (EAS) increases progesterone synthesis in bovine granulosa cells through the activation of the steroidogenesis pathway and improvement of mitochondrial activity and lipid metabolism. This effect is mediated by the induction of heat shock protein 70 (HSP70) and the enhancement of intracellular redox balance, and shows a synergistic effect with HSP70-regulated steroidogenesis pathway.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Gabriel Vasata Furtado, Jing Yang, Di Wu, Christos Papagiannopoulos, Hanna M. Terpstra, E. F. Elsiena Kuiper, Sybille Krauss, Wei-Guo Zhu, Harm H. Kampinga, Steven Bergink
Summary: FOXO1 prevents toxic polyQ aggregation and reduces the synthesis rate of proteins from pathogenic CAG repeat transcripts, thereby decreasing the initiation of amyloidogenesis in CAG-repeat diseases.
HUMAN MOLECULAR GENETICS
(2021)
Article
Multidisciplinary Sciences
Henry Reyer, Siriluck Ponsuksili, Ellen Kanitz, Ralf Poehland, Klaus Wimmers, Eduard Murani
Article
Reproductive Biology
Mirela B. Souza Caceres, Wilian A. Leite da Silva, Ana C. Bini de Lima, Jair S. de Oliveira, Christopher J. Tavares Cardoso, Jonathan V. dos Santos, Evelyn R. Andrade, Mauricio M. Franco, Ralf Poehland, Fabiana de Andrade Melo Sterza
Article
Cell Biology
Falko Lange, Bettina Brandt, Markus Tiedge, Ludwig Jonas, Udo Jeschke, Ralf Poehland, Hermann Walzel
HISTOCHEMISTRY AND CELL BIOLOGY
(2009)
Article
Endocrinology & Metabolism
Thomas Laeger, Ralf Poehland, Cornelia C. Metges, Bjoern Kuhla
JOURNAL OF ENDOCRINOLOGY
(2012)
Article
Biochemistry & Molecular Biology
Falko Lange, Katja Rateitschak, Brit Fitzner, Ralf Poehland, Olaf Wolkenhauer, Robert Jaster
Article
Biochemistry & Molecular Biology
R. Poehland, W. Tomek, F. Becker, J. Kurth, W. Kanitz, S. Bhojwani
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2008)
Article
Toxicology
U. Tiemann, K. -P. Bruessow, D. Dannenberger, L. Jonas, R. Poehland, K. Jaeger, S. Daenicke, E. Hagemann
TOXICOLOGY LETTERS
(2008)
Article
Veterinary Sciences
U. Tiemann, K. -P. Bruessow, U. Muechenmeister, L. Jonas, R. Poehland, A. Reischauer, K. Jaeger, S. Daenicke
VETERINARY JOURNAL
(2008)
Article
Reproductive Biology
M. B. Souza-Cacares, A. L. L. Fialho, W. A. L. Silva, C. J. T. Cardoso, R. Poehland, M. I. M. Martins, F. A. Melo-Sterza
Article
Agriculture, Dairy & Animal Science
Wilian Aparecido Leite da Silva, Ralf Poehland, Caroline Carvalho de Oliveira, Mariane Gabriela Cesar Ribeiro Ferreira, Ricardo Garcia de Almeida, Mirela Brochado Souza Caceres, Gustavo Guerino Macedo, Eliane Vianna da Costa e Silva, Fabiana Villa Alves, Eriklis Nogueira, Fabiana de Andrade Melo-Sterza
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2020)
Article
Cell Biology
Daniela Moraes Pereira, Christopher Junior Tavares Cardoso, Wilian Aparecido Leite da Silva, Mirela Brochado Souza-Caceres, Mariana Santos, Ralf Pohland, Allan Motta Couto, Iluska Senna Bonfa Moslaves, Monica Cristina Toffoli Kadri, Fabiana de Andrade Melo Sterza
Review
Biochemistry & Molecular Biology
Fabiana de Andrade Melo-Sterza, Ralf Poehland
Summary: Lipids play a crucial role in the initial development of embryos, but excessive lipid droplet formation may be harmful to embryo cryotolerance. Additionally, increased lipid accumulation in in vitro produced embryos may be a response to stress, and there are still many questions regarding embryo lipid metabolism and developmental potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Ralf Poehland, Mirela Brochado Souza-Cacares, Tirtha Kumar Datta, Jens Vanselow, Maria Isabel Mello Martins, Wilian Aparecido Leite da Silva, Christopher Junior Tavares Cardoso, Fabiana de Andrade Melo-Sterza
ANIMAL REPRODUCTION
(2020)
Article
Agriculture, Dairy & Animal Science
Ralf Poehland, Fatima Al-Rostum, Frank Becker, Torsten Viergutz, Ronald Marco Brunner, Wilhelm Kanitz, Sanjay Bhojwani
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2007)