Article
Pharmacology & Pharmacy
Namyoung Kang, Le Thi Thuy, Viet Dongquoc, Joon Sig Choi
Summary: A new functional polymer, FKFL-G2, was synthesized by joining a short oligopeptide sequence (Phe-Lys-Phe-Leu, FKFL) to the surface of a second-generation polyamidoamine (G2 PAMAM) dendrimer as an antibacterial component. FKFL-G2 showed low toxicity to noncancerous cells and exhibited antibacterial effects on Escherichia coli and Staphylococcus aureus strains. These findings suggest that FKFL-G2 has potential as an antibacterial agent.
Article
Biochemistry & Molecular Biology
Wafa K. Fatani, Fadilah S. Aleanizy, Fulwah Y. Alqahtani, Mohammed M. Alanazi, Abdullah A. Aldossari, Faiyaz Shakeel, Nazrul Haq, Hosam Abdelhady, Hamad M. Alkahtani, Ibrahim A. Alsarra
Summary: Lung cancer is the leading cause of cancer-related deaths worldwide. Erlotinib, a tyrosine kinase inhibitor, has been explored as a potential treatment for lung cancer. Researchers have formulated two different generations of polyamidoamine (PAMAM) dendrimers and evaluated their efficacy in delivering erlotinib. The results showed that the cationic G4 dendrimer had higher selectivity and antiproliferative activity against lung cancer cells compared to the neutral G5 dendrimer and erlotinib alone, suggesting its potential as a targeted and sustained release carrier for erlotinib.
Article
Environmental Sciences
Soyeon Jeon, Dong-Keun Lee, Jiyoung Jeong, Sung Ik Yang, Ji-Su Kim, Jinsik Kim, Wan-Seob Cho
Summary: This study investigated the toxicity of microplastics on different cell lines and found that microplastics showed significant toxicity to THP-1 macrophages, with toxicity correlated to the generation potential of reactive oxygen species (ROS). Weathering process reduced the ROS generation potential of microplastics, as weathered microplastics had increased affinity to bind serum protein acting as ROS scavenger.
ENVIRONMENTAL POLLUTION
(2021)
Article
Chemistry, Physical
Patryk Szymaszek, Patrycja Sroda, Malgorzata Tyszka-Czochara, Anna Chachaj-Brekiesz, Tomasz Swiergosz, Joanna Ortyl
Summary: Here we report the synthesis of a novel fluorescent probe P182 for quantitative measurement of H2O2. This fluorometric probe displays a fluorescence turn-on response in the presence of H2O2. It offers good performances in terms of sensitivity and response time, and shows non-toxic activity for in vivo studies. This study provides research on molecular fluorescence probe for the detection of H2O2.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Materials Science, Biomaterials
Yalcin Bukun, Merve Zaim, Mehmet Senel, Tugba Sagir, Bercem Yaman Kiyak, Sevim Isik
Summary: Fluorescent dyes enable visualization of biological molecules, and FITC-PAMAM dendrimers have been synthesized as a new fluorescent agent for drug delivery study. The structural efficacy of FITC-PAMAM dendrimers was characterized and the drug loading behavior and antiproliferative activity were evaluated. FITC-PAMAM dendrimers facilitated the entry of 5-FU into cells and exhibited generation-dependent inhibition of cell proliferation.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Nerilson M. Lima, Gagan Preet, Sara R. Marqui, Thais de O. R. S. Falcoski, Geovana Navegante, Christiane P. Soares, Teresinha de Jesus A. S. Andrade, Felipe A. La Porta, Harinantenaina Liva R. Rakotondraie, Marcel Jaspars, Dulce H. S. Silva
Summary: This study evaluated the metabolic profiling and antigenotoxicity of Inga species with potential antitumor effects. It also used a proteomic approach to investigate the response of HepG2 cells to polyphenols isolated from I. laurina. The results showed cytotoxic activity against cancer cell lines and identified potential bioactive compounds. The proteomic analysis revealed proteins related to ROS elimination, DNA repair, tumor protein expression, and apoptosis.
Article
Biochemistry & Molecular Biology
Yuri Shatalin, Victoria S. Shubina, Marina E. Solovieva, Vladimir S. Akatov
Summary: The oxidation of thiols catalyzed by hydroxocobalamin (HOCbl) and cyanocobalamin (CNCbl) leads to reactive oxygen species (ROS) production and oxidative stress. The mechanisms and kinetics of thiol oxidation differ between HOCbl and CNCbl, affecting oxidation rate and ROS quenching. These findings provide new insight into the redox processes involving cobalamins and may contribute to the development of treatment approaches for oxidative stress-related cobalamin-responsive disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Younjin Kim, Joon-Sig Choi
Summary: New hybrid polymers composed of polyamidoamine dendrimer generation 2 (PAMAM G2) and KKRK, KKRKH peptides derived from a nuclear localization sequence (NLS) were synthesized and found to significantly enhance gene transfection efficiency as gene carriers. The main endocytosis mechanism was identified as macropinocytosis and the polyplexes were observed to exist around the cell nucleus. Additionally, the polyplexes showed active escape from endosomes.
Review
Biochemistry & Molecular Biology
Loshini Soundararajan, Sudha Warrier, Arun Dharmarajan, Natarajan Bhaskaran
Summary: Reactive oxygen species (ROS) and its related signaling pathways and regulating molecules play a major role in the growth and development of cancer stem cells. CSCs possess robust self-renewal and tumorigenic potential, are resistant to conventional chemo- and radiotherapy, and eradicating CSCs may be the key to curing cancer. However, CSCs efficiently expel ROS by upregulating ROS scavengers, protecting them from ROS-mediated cell death and leading to chemoresistance and radioresistance.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Shanshan Gou, Lu Qiu, Qiufang Yang, Peng Li, Xiaowen Zhou, Yixuan Sun, Xiuman Zhou, Wenshan Zhao, Wenjie Zhai, Guodong Li, Yahong Wu, Yonggang Ren, Yuanming Qi, Yiguo Zhang, Yanfeng Gao
Summary: Metformin induces ROS accumulation by inhibiting the expression of the core antioxidant transcription factor NFE2L1, which can be reversed by high glucose. The inhibitory effect of metformin on NFE2L1 is AMPK-independent, and NFE2L1 may play a crucial role in regulating the AMPK signaling pathway.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Review
Nanoscience & Nanotechnology
Anton Tkachenko, Anatolii Onishchenko, Valeriy Myasoedov, Svetlana Yefimova, Ondrej Havranek
Summary: Nanomedicine is a rapidly growing field of nanotechnology. Lack of standardized toxicity screening protocols is a major obstacle in the use of nanomaterials for medical applications. This review focuses on less studied regulated cell death (RCD) modalities induced by nanomaterials, summarizing the mechanisms and suggesting that ROS overproduction and pathways play a central role. Evaluation of RCDs can become a mechanism-based screening method in nanotoxicology and provide useful information for medical applications and anti-tumor potential of nanomaterials.
Article
Environmental Sciences
Le Yu, Zhuang Wang, De-Gao Wang
Summary: This study investigated the toxicological effects of two typical layered double hydroxides (LDHs) on freshwater green algae and the main factors affecting their toxicity. The results showed that Zn-Al-LDH exhibited stronger growth inhibition toxicity than Mg-Al-LDH, which was attributed to the stability of particle dispersion and metallic composition. The dissolved metal ions contributed less to the overall toxicity compared to particulate forms, and the joint toxic action of different dissolved metal ions belonged to additive effects. The LDHs caused severe damage to the algal cell surface and induced oxidative stress in the cells.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Mohd Jahir Khan, Abrar Ahmad, Mahmood Ahmad Khan, Sahabjada Siddiqui
Summary: This study investigated the anticancer properties of zinc oxide nanoparticles (ZnO-NPs) on human epidermoid carcinoma cells and found that ZnO-NPs induce apoptosis in A431 cells through DNA degradation and generation of reactive oxygen species via p53, bax/bcl-2, and caspase pathways.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Aazam Shaikh, Prashant Kesharwani, Virendra Gajbhiye
Summary: Dendrimers have a wide range of applications in biomedical fields, including cargo delivery, nucleic acid delivery, and macromolecule delivery. They can also be used to crosslink synthetic and natural polymeric scaffolds and mimic the extracellular matrix. Dendrimers play a crucial role in regulating cell behavior and delivering bioactive molecules, and have shown significant results in stem cell survival, osteogenesis, and repair.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Biochemistry & Molecular Biology
Li-Chiu Yang, Yu-Chao Chang, Kun-Lin Yeh, Fu-Mei Huang, Ni-Yu Su, Yu-Hsiang Kuan
Summary: This study investigated the protective mechanism of rutin in alleviating TEGDMA-induced toxicity in macrophages. Results showed that rutin substantially reduced cytotoxicity, apoptosis, and genotoxicity, and decreased oxidative stress and ROS generation caused by TEGDMA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Cristina Fuentes, Ana Fuentes, Hugh J. Byrne, Jose Manuel Barat, Maria Jose Ruiz
Summary: The cytotoxicity of carvacrol and thymol-functionalised mesoporous silica microparticles was evaluated in human hepatocarcinoma cells, which showed that the materials induced toxicity by causing oxidative stress and resulting in apoptosis.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Review
Chemistry, Analytical
S. Bielfeldt, F. Bonnier, H. J. Byrne, I. Chourpa, Y. Dancik
Summary: The study of human skin is significant in various fields including dermatology, toxicology, pharmacology, and cosmetology. Current measurement methods do not provide chemically specific results regarding skin penetration and permeation. However, Raman microspectroscopy can serve as an alternative method for non-destructive, non-invasive, and chemically specific investigations.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Joy Udensi, James Loughman, Ekaterina Loskutova, Hugh J. Byrne
Summary: This review discusses the structural and optical properties of carotenoid compounds and the use of Raman spectroscopy to identify carotenoids in biological environments. It focuses on applications in nutritional analysis, optometry, and serology, and explores the progress and challenges in establishing the technique in a clinical environment.
Article
Chemistry, Multidisciplinary
Xinyi Zhao, Gwendoline Smith, Bilal Javed, Garret Dee, Yurii K. Gun'ko, James Curtin, Hugh J. Byrne, Christine O'Connor, Furong Tian
Summary: The study developed two methods using nanoparticles and antibodies for detecting Salmonella. The ICGNP method showed higher sensitivity and specificity, providing effective detection of Salmonella.
Review
Endocrinology & Metabolism
Nitin Patil, Orla Howe, Paul Cahill, Hugh J. Byme
Summary: This paper thoroughly discusses the medical relevance of the cellular glycolytic pathway and explores the current state of monitoring and modelling its dynamics. The potential of label-free vibrational microspectroscopic techniques to overcome the limitations of current approaches is presented.
MOLECULAR METABOLISM
(2022)
Article
Marine & Freshwater Biology
Gabriela H. Da Silva, Lidiane Silva Franqui, Marcelo A. De Farias, Vera Lucia S. S. De Castro, Hugh J. Byrne, Diego S. T. Martinez, Regina T. R. Monteiro, Alan Casey
Summary: This study investigated the cytotoxicity, protein corona formation, and cellular internalisation of TiO2_MWCNT on RTG-2 cells for the first time. The results showed that this nanohybrid had no toxicity effects on RTG-2 cells within 24 hours. Spectral and microscopic analysis revealed that TiO2_MWCNT can form complexes through adsorption of proteins and be internalised by cells.
AQUATIC TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shane Forde, Giulianna Vozza, David J. Brayden, Hugh J. Byrne, Jesus M. Frias, Sinead M. Ryan
Summary: In order to address the challenges of oral administration of SeMet, researchers encapsulated it in zein-coated nanoparticles made from chitosan using an ionic gelation formulation. The SeMet-NPs showed high stability and minimal cytotoxicity, as well as antioxidant properties. Additionally, they exhibited increased intestinal permeability compared to free SeMet. These findings suggest the potential of SeMet-NPs for further development as an oral supplement.
Article
Chemistry, Multidisciplinary
Furong Tian, Luis Felipe das Chagas e Silva de Carvalho, Alan Casey, Marcelo Saito Nogueira, Hugh J. Byrne
Summary: In recent years, the disease burden of hyperuricemia has been increasing, especially in high-income countries and the economically developing world with a Western lifestyle. Improved methods of uric acid and hypoxanthine detection using surface-enhanced Raman spectroscopy (SERS) and high-performance liquid chromatography (HPLC) were demonstrated by analyzing three different bodily fluids. Gold nanostars were used for quantitative detection of uric acid and hypoxanthine concentrations. The results show strong correlations between HPLC and SERS, and the potential of vibrational spectroscopy in clinical applications was highlighted.
Article
Pharmacology & Pharmacy
Suha Elderderi, Franck Bonnier, Xavier Perse, Hugh J. Byrne, Florent Yvergnaux, Igor Chourpa, Abdalla A. Elbashir, Emilie Munnier
Summary: Raman spectroscopy is a useful technique for quantifying complex cosmetic products without the need for extensive pre-analytical processing. This study demonstrates the effectiveness of Raman spectroscopy coupled with partial least squares regression (PLSR) in quantifying Alginate nanoencapsulated Piperonyl Esters (ANC-PE) in a hydrogel. By dividing the samples into a training set and a test set, the accuracy of the prediction model was evaluated, with a root mean square error of cross-validation (RMSECV) and prediction (RMSEP) of 0.142% and 0.148% (w/w PE), respectively. These results highlight the potential of Raman spectroscopy for rapid and label-free quantification of active cosmetic ingredients in complex formulations, promising future applications in analytical quality control (AQC).
Article
Chemistry, Analytical
Maria Joao Oliveira, Soraia Caetano, Ana Dalot, Filipe Sabino, Tomas R. Calmeiro, Elvira Fortunato, Rodrigo Martins, Eulalia Pereira, Miguel Prudencio, Hugh J. Byrne, Ricardo Franco, Hugo Aguas
Summary: Early and accurate detection of pathogenic microorganisms is crucial for diagnosis and patient outcomes. The combination of a polystyrene-based microfluidic device and Surface-Enhanced Raman Spectroscopy shows excellent sensitivity and specificity in detecting malaria. The system can be easily adapted for other pathogens and has the potential for early diagnosis of infectious diseases.
Article
Chemistry, Analytical
Valentina Notarstefano, Alessia Belloni, Paolo Mariani, Giulia Orilisi, Giovanna Orsini, Elisabetta Giorgini, Hugh J. J. Byrne
Summary: In this study, Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) and Principal Component Analysis (PCA) were used to analyze Raman MicroSpectroscopy (RMS) data of Oral Squamous Carcinoma Cells (OSCC) exposed to the chemotherapeutic agent cisplatin. The results showed that RMS coupled with multivariate analysis can quantitatively monitor the progression of cellular responses to different drugs, providing potential applications for label-free, in vitro, pre-clinical screening.
Review
Endocrinology & Metabolism
Zohreh Mirveis, Orla Howe, Paul Cahill, Nitin Patil, Hugh J. J. Byrne
Summary: In recent years, the analysis of the glutamine metabolic pathway has been a major focus in metabolomics research due to its significance in cell biosynthesis and bioenergetics, especially in cancer cell survival. However, monitoring the kinetics of this pathway in a living cell in real-time and holistically remains a challenge.
Article
Materials Science, Multidisciplinary
Aimee Sheehan, Tatsiana Mikulchyk, Catherine S. P. De Castro, Safakath Karuthedath, Wejdan Althobaiti, Metodej Dvoracek, Hugh J. Sabad-e-Gul, Hugh J. Byrne, Frederic Laquai, Izabela Naydenova, Mikhail A. Filatov
Summary: A series of new heavy-atom-free photosensitizers were designed and applied for holographic recording in a photopolymerizable material. The photoinduced electron transfer and spin-orbit charge transfer intersystem crossing were studied, and highly efficient photosensitization was observed.
JOURNAL OF MATERIALS CHEMISTRY C
(2023)
Article
Biochemistry & Molecular Biology
Minna Khalid Danish, John P. Gleeson, David J. Brayden, Hugh J. Byrne, Jesus M. Frias, Sinead M. Ryan
Summary: This study demonstrates the potential of chitosan-zein nanoparticles as an oral delivery system for the encapsulation of IPP and LKP, showing their effectiveness in reducing blood pressure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Suha Elderderi, Soukaina Hilali, Laura Wils, Igor Chourpa, Martin Souce, Barbara Clement-Larosiere, Abdalla A. Elbashir, Hugh J. Byrne, Emilie Munnier, Leslie Boudesocque-Delaye, Franck Bonnier
Summary: The study evaluated the feasibility of using ATR-IR and PLSR as a rapid, label-free, and cost-effective tool to quantify water content in extracts from spirulina wet biomass obtained using NADES. The results highlighted the accuracy and reliability of the technique in quantitative analysis, offering powerful alternatives for developing more environmentally responsible analytical protocols.
ANALYTICAL METHODS
(2022)
Article
Toxicology
Kathryn Page, Walter Westerink, Kristie Sullivan, Thomas McDonald, Clive Roper
Summary: This study developed a new method to assess the skin irritation of antimicrobial cleaning products. The method utilized a more human-like model and demonstrated its effectiveness through comparison with in vivo rabbit skin irritation data.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Arupam Patra, Thirukumaran Kandasamy, Siddhartha Sankar Ghosh, Gurvinder Kaur Saini
Summary: This study successfully produced recombinant anisoplin and demonstrated its significant anti-cancer effect and ability to induce apoptosis in breast cancer cells. The activation of related signaling pathways may be the key to cell death.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Thomas Haller, Alexander Jesacher, Alberto Hidalgo, Christina Schmidt
Summary: This study used live cell imaging to observe the accumulation of amiodarone in primary rat alveolar type II cells, and found that it specifically accumulates in lamellar bodies. The uptake is rapid, while storage is persistent. The main mechanisms for intracellular bioaccumulation of amiodarone are proposed to be passive diffusion, ion-trapping, and lipophilic interactions.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Filiz Bakar-Ates, Erva Ozkan
Summary: This study investigated the ferroptotic effect of CuB in breast cancer cells and evaluated its combination with erastin, a ferroptosis inducer. The results showed that the combination treatment significantly activated the ferroptotic pathways and altered the expression of iron-related proteins in breast cancer cells.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Rachel Z. Bennie, Ian C. Shaw
Summary: Estrogen mimics are synthetic and naturally occurring compounds that can interact with estrogen receptors in animals. In vitro transactivation reporter gene assay and in silico molecular modelling can be used to predict the mimicry of these compounds, reducing reliance on animal studies.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Sevda Isik, Semra Cicek
Summary: This study found that high doses of riboflavin can cause cytotoxicity in rainbow trout gonad cells and affect the transcriptional expressions of antioxidant enzymes and growth and reproductive genes, potentially leading to DNA damage and cell death.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Ying Wang, Jie Wu, Mengqi Wan, Dou Yang, Fasheng Liu, Kehao Li, Manxin Hu, Yuanyuan Tang, Huiqiang Lu, Shouhua Zhang, Yuanzhen Xiong
Summary: m-Cresol is commonly used as an intermediate for pesticides and other industrial applications. This study investigated the hepatotoxicity of m-cresol using zebrafish larvae and explored its molecular mechanisms. The results suggest that m-cresol may induce liver damage in zebrafish larvae through oxidative stress and cell apoptosis pathways.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Vincent J. Manna, Shannon Dwyer, Vanessa Pizutelli, Salvatore J. Caradonna
Summary: The widespread use of electronic cigarettes and the emergence of a new illness have raised concerns about the effects of e-cigarette vapor on respiratory tissues. Researchers have developed a simple device to mimic the response of human airway tissue after long-term exposure to e-cigarette vapor, and have identified differences in the effects of different vapor compositions on airway tissue.
TOXICOLOGY IN VITRO
(2024)