期刊
TOXICOLOGY IN VITRO
卷 25, 期 8, 页码 1981-1988出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2011.06.022
关键词
Zearalenone; alpha-Zearalenol; beta-Zearalenol; Zearalanone; Swine PBMC; Immunity
类别
资金
- CNCSIS - UEFISCDI [PNII - IDEI 1373/2008]
- Romanian Ministry of Research and Technology [PNII - PARTENE-RIATE 52122/2008]
- [POSDRU/89/1.5/S/63258]
Zearalenone (ZEN), a mycotoxin produced by several Fusarium spp., is most commonly found as a contaminant in stored grain and has chronic estrogenic effects on mammals. In this in vitro study, we compared the effects of zearalenone (ZEN) and some of its derivatives: alpha-zearalenol (alpha-ZOL), beta-zearalenol (beta-zoL), and zearalanone (ZAN) on several peripheral blood mononuclear cell (PBMC) parameters: cytotoxicity, proliferation, as well as antibody and cytokine synthesis. The amounts of toxins necessary to inhibit viability, in a dehydrogenase enzyme activity assay (MTT test), by 50% were: 22.7 mu M for ZEN, 29.1 mu M for alpha-ZOL, 17.3 mu M for beta-ZOL and 26.3 mu M for ZAN. The administration of 10 mu M toxin induced a decrease in the ConA stimulated proliferation of PBMC by 19.6% for ZAN, 45.4% for ZEN, 43.6% for alpha-ZOL and 85.2% for beta-ZOL, when compared to the control stimulated cells. Also. ZEN and its metabolites at concentrations higher than 5 mu M induced a significant decrease of the IgG, IgA or IgM levels. Concentrations of 5 and 10 mu M of ZEN and ZAN significantly decreased the TNF-alpha synthesis in the supernatant of the stimulated cells; 10 mu M of ZAN also decreased IL-8 synthesis. In conclusion, our results show that ZEN and ZEN derivatives altered several parameters of the humoral and cellular immune response. Therefore, our results are clinically relevant as ZEN and its metabolites are frequent contaminants of animal feed and we have shown that intoxicated animals are incapable of inducing an adequate immune response. (C) 2011 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据