期刊
TOXICOLOGY IN VITRO
卷 25, 期 1, 页码 153-159出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2010.10.010
关键词
Bisphenol A; Genistein; Micromass; Developmental toxicity; Combined effect
类别
资金
- National Natural Sciences Foundations of the People's Republic of China [30771821, 30972501]
- Key Projects in the National Science and Technology Pillar Program in the Eleventh Five-year Plan Period [2006BKA02A02]
- National Science and Technology Major Specific Project [2009ZX09301-010]
Bisphenol A (BPA), widely used in industry and dentistry, and genistein (GEN), the predominant component of soy product, are both known environmental estrogen. In the present study, we investigated the developmental toxicity of BPA and GEN and their combined effect using micromass test, which is one of three standard alternative developmental toxicity tests recommended by European Center for the Validation of Alternative Methods (ECVAM). The results showed that IC50-P (cell proliferation) and IC50-D (cell differentiation) of BPA and GEN were approximately 20 and 5 mu g/ml, respectively. No observed adverse effect level (NOAEL) of BPA and GEN were 10 and 0.94 mu g/ml, respectively. The manifestation of BPA as a teratogen was insufficient, although the low dose effect should be paid attention to. While the evidence of GEN as a teratogen was solid, especially with the consideration of high dose application in clinical treatment. The combined effect of BPA and GEN was generally additive action except that in MB proliferation. (C) 2010 Elsevier Ltd. All rights reserved.
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