期刊
PLOS GENETICS
卷 11, 期 5, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pgen.1005225
关键词
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资金
- National Institutes of Health of the United States of America [T32 CA009657, R01 GM032194]
- Slovak Research and Development Agency [2/0014/14, APVV-0111-12]
- Austrian Science Foundation [P21437, P23609, PCIG11-GA-2012-322300, APVV-0334-12, 1/0196/14]
- European Commission
- Slovak Research and Development Agency
- Austrian Science Fund (FWF) [P21437] Funding Source: Austrian Science Fund (FWF)
- Austrian Science Fund (FWF) [P 23609, P 21437] Funding Source: researchfish
Proper meiotic chromosome segregation, essential for sexual reproduction, requires timely formation and removal of sister chromatid cohesion and crossing-over between homologs. Early in meiosis cohesins hold sisters together and also promote formation of DNA double-strand breaks, obligate precursors to crossovers. Later, cohesin cleavage allows chromosome segregation. We show that in fission yeast redundant casein kinase 1 homologs, Hhp1 and Hhp2, previously shown to regulate segregation via phosphorylation of the Rec8 cohesin subunit, are also required for high-level meiotic DNA breakage and recombination. Unexpectedly, these kinases also mediate phosphorylation of a different meiosis-specific cohesin subunit Rec11. This phosphorylation in turn leads to loading of linear element proteins Rec10 and Rec27, related to synaptonemal complex proteins of other species, and thereby promotes DNA breakage and recombination. Our results provide novel insights into the regulation of chromosomal features required for crossing-over and successful reproduction. The mammalian functional homolog of Rec11 (STAG3) is also phosphorylated during meiosis and appears to be required for fertility, indicating wide conservation of the meiotic events reported here.
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