期刊
PLOS GENETICS
卷 11, 期 12, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pgen.1005704
关键词
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资金
- 973 plan [2012CB114001]
- National Nature Science Foundation of China [31270179, 31070124, 31030004, 31328002]
- scientific research foundation of graduate school of FAFU
- Temasek Life Sciences Laboratory
- National Research Foundation, Singapore
- 973 plan [2012CB114001]
- National Nature Science Foundation of China [31270179, 31070124, 31030004, 31328002]
- scientific research foundation of graduate school of FAFU
- Temasek Life Sciences Laboratory
- National Research Foundation, Singapore
The retromer mediates protein trafficking through recycling cargo from endosomes to the trans-Golgi network in eukaryotes. However, the role of such trafficking events during pathogen-host interaction remains unclear. Here, we report that the cargo-recognition complex (MoVps35, MoVps26 and MoVps29) of the retromer is essential for appressorium-mediated host penetration by Magnaporthe oryzae, the causal pathogen of the blast disease in rice. Loss of retromer function blocked glycogen distribution and turnover of lipid bodies, delayed nuclear degeneration and reduced turgor during appressorial development. Cytological observation revealed dynamic MoVps35-GFP foci co-localized with autophagy-related protein RFP-MoAtg8 at the periphery of autolysosomes. Furthermore, RFP-MoAtg8 interacted with MoVps35-GFP in vivo, RFP-MoAtg8 was mislocalized to the vacuole and failed to recycle from the autolysosome in the absence of the retromer function, leading to impaired biogenesis of autophagosomes. We therefore conclude that retromer is essential for autophagy-dependent plant infection by the rice blast fungus.
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