期刊
TOXICOLOGY AND INDUSTRIAL HEALTH
卷 24, 期 4, 页码 227-234出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/0748233708095862
关键词
chromosome; in-vitro maturation; oocytes; spindle; thioglycolic acid
资金
- Chinese Academy of Sciences
- National Nature Science Foundation of China [30400356]
Previous studies have shown that thioglycolic acid (TGA) leads to potential reproductive toxicology. To clarify the exact effects of this compound on reproduction, mice oocytes were treated with different TGA doses. At the end of the culture period, the nuclear status of mice oocytes was assessed under an inverted microscope. After immunofluorescence staining, the chromosomal arrangement and spindle configuration of oocytes were evaluated. The results indicated that TGA decreases the percentage of first polar body formation but does not influence that of germinal vesicle breakdown. TGA induces abnormal chromosomal arrangement and spindle elongation. In conclusion, TGA inhibits in-vitro maturation of mice oocytes and affects chromosomal arrangement and spindle configuration. Furthermore, it probably interferes with biochemical changes that occur during meiosis, resulting in aberrant development. Toxicology and Industrial Health 2008; 24: 227-234.
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