Article
Environmental Sciences
Ramses Santacruz-Marquez, Jodi A. Flaws, Luz del Carmen Sanchez-Pena, Isabel Hernandez-Ochoa
Summary: The use of zinc oxide nanoparticles may potentially affect the functions of ovarian antral follicles, leading to increased estrogen production and an antioxidant response.
Article
Reproductive Biology
Malgorzata Grzesiak, Katarzyna Knapczyk-Stwora, Maria Slomczynska
Summary: Recent studies have shown that vitamin D-3 plays a crucial role in regulating ovarian function in pigs, with the expression of VD-related molecules varying among follicle sizes and developmental stages. The findings suggest that porcine antral follicles are directly affected by VD action, influencing steroidogenesis and potentially playing a role in follicular development and function.
Article
Biochemistry & Molecular Biology
Fu Wei, Xueying Fan, Julieta S. del Valle, Joyce D. Asseler, Lotte E. van der Meeren, Hui Cheng, Bernard A. J. Roelen, Leoni A. Louwe, Gonneke S. K. Pilgram, Lucette A. J. van der Westerlaken, Norah M. van Mello, Susana M. Chuva de Sousa Lopes
Summary: This study investigates the characteristics of atretic small antral follicles in the ovary and proposes a classification system based on molecular changes observed in granulosa cells, theca cells, and extracellular matrix deposition. The results show that atresia spreads in the follicle with wave-like dynamics and CD68+ macrophages are enriched during the progression of follicular atresia. This study provides insights into follicular degeneration and its potential implications for preventing or treating premature ovarian failure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xueying Fan, Ioannis Moustakas, Monika Bialecka, Julieta S. del Valle, Arend W. Overeem, Leoni A. Louwe, Gonneke S. K. Pilgram, Lucette A. J. van der Westerlaken, Hailiang Mei, Susana M. Chuva de Sousa Lopes
Summary: This study examines the transcriptional signatures of human oocytes and granulosa cells from small antral follicles, revealing the diversity of follicle types and contributing to the understanding of human folliculogenesis and oogenesis at the molecular level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Toxicology
Vasiliki E. Mourikes, Ramses Santacruz-Marquez, Ashley Deviney, Mary J. Laws, Alexander Ulanov, Michael R. La Frano, Jodi A. Flaws
Summary: This study confirmed that ovarian follicles can metabolize and bioactivate the neonicotinoid insecticide IMI, providing the first evidence of its metabolism in the ovaries. The results showed that IMI can produce bioactivated metabolites in follicles and induce the expression of specific metabolic enzymes in the ovaries over time.
TOXICOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lu-Yang Jin, Jia-En Yu, Hai-Yan Xu, Bin Chen, Qian Yang, Ye Liu, Meng-Xi Guo, Cheng-Liang Zhou, Yi Cheng, Hai-Yan Pang, Hai-Yan Wu, Jian-Zhong Sheng, He-Feng Huang
Summary: This study identified the potential role of the Pde4d gene in polycystic ovary syndrome. Experimental validation showed that overexpression of Pde4d led to the formation of ovarian cysts and inhibited follicle maturation and apoptosis. This finding is important for understanding the pathogenesis of polycystic ovary syndrome.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Reproductive Biology
Abigail M. Maucieri, David H. Townson
Summary: O-GlcNAcylation occurs in granulosa cells of small and large bovine antral follicles, and its inhibition impairs granulosa cell proliferation.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Emmi Rotgers, Barbara Nicol, Karina Rodriguez, Saniya Rattan, Jodi A. Flaws, Humphrey Hung-Chang Yao
Summary: The study found that overexpression of NR5A1 in female mice leads to reproductive dysfunction and metabolic imbalance, resulting in premature infertility. These results provide a new model for studying not only the molecular actions of NR5A1, but also the crosstalk between endocrine, reproductive, and metabolic systems.
Article
Toxicology
Katie L. Land, Madison E. Lane, Ava C. Fugate, Patrick R. Hannon
Summary: This study investigated the effects of phthalates on ovulation and found that phthalates can directly inhibit ovulation by decreasing prostaglandin levels, altering progesterone/PGR action, and affecting extracellular matrix remodeling.
TOXICOLOGICAL SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Y. Wang, Z. Y. Guo, C. Zhang, D. Z. Miao, X. Y. Mao, S. M. Lu, H. M. Yang, Z. Y. Wang
Summary: This study investigated the development of ovarian follicles and the concentrations of steroid hormones in paired pigeons during the laying interval. The results showed that paired pigeons normally have 2 preovulatory follicles, with the second-largest follicle developing from the third day of the laying interval. The concentrations of P4 and T showed specific patterns during the laying interval, while E2 remained at high levels. The findings provide important insights into the regulation of ovulation and egg production in pigeons.
Article
Biochemistry & Molecular Biology
Patrycja Witek, Malgorzata Grzesiak, Marek Koziorowski, Maria Slomczynska, Katarzyna Knapczyk-Stwora
Summary: In this study, the effects of neonatal exposure to the insecticide methoxychlor (MXC) on ovarian follicles in adult pigs were investigated. The results suggest that MXC exposure may have long-term effects on the signaling pathways involved in follicle development in the ovaries of pigs. However, the effects of MXC vary at different stages of follicular development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Obstetrics & Gynecology
Barbara Rodrigues Nascimento, Danielle Storino de Freitas, Julia Meireles Nogueira, Clara Carvalho e Souza, Rayan Silva de Paula, Jousie Michel Pereira, Ana Paula Madureira, Luciola Silva Barcelos, Erika Cristina Jorge, Paulo Henrique Almeida Campos-Junior
Summary: Ovarian cryopreservation is a viable option for fertility preservation and subcutaneous transplantation is a promising site. This study aimed to evaluate the effect of subcutaneous autotransplantation and vitrification on follicular growth and atresia, as well as their relationship with vascular perfusion and gene expression. The results showed that the first day after subcutaneous transplantation is critical for follicular survival, with intense follicular atresia independent of Bax upregulation.
REPRODUCTIVE SCIENCES
(2023)
Article
Environmental Sciences
Andressa Gonsioroski, Mary Laws, Vasiliki E. Mourikes, Alison Neff, Jenny Drnevich, Michael J. Plewa, Jodi A. Flaws
Summary: This study identified differentially expressed genes and pathways involved in the toxic effects of iodoacetic acid (IAA) on ovarian follicles. Exposure to IAA altered gene expression and decreased estradiol levels in mice.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2022)
Article
Veterinary Sciences
Renato F. Silva, Laritza F. Lima, Rebeca M. P. Rocha, Ivina R. Brito, Gerlane M. Silva, Hudson H. Correia, Giovanna Q. Rodrigues, Anna C. A. Ferreira, Diana C. S. Nunes-Pinheiro, Arlindo A. A. N. Moura, Larissa B. R. Silveira, Edson G. Lo Turco, Matthew B. Wheeler, Ana P. R. Rodrigues, Claudio C. Campello, Jose R. Figueiredo
Summary: Ethanol treatment increased degeneration rates of follicles and oocytes, but led to higher estradiol secretion and meiotic resumption. It decreased mRNA levels of certain genes but increased others. The metabolomic profiles of follicles were similar between ethanol and rbFSH treatments, different from the Control treatment.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sarah Rudzinskas, Jessica F. Hoffman, Pedro Martinez, David R. Rubinow, Peter J. Schmidt, David Goldman
Summary: The study found that women with PMD are more sensitive to changes in E2 at the cellular level, leading to differential behavioral responses. Transcriptome analysis revealed significant differences in gene expression in PMD patients in all experimental conditions, suggesting that CXCL10 and CYP7B1 may serve as biomarkers and molecular targets for intervention in PMD.
MOLECULAR PSYCHIATRY
(2021)
Editorial Material
Reproductive Biology
Zelieann R. Craig, Bernard Robaire
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Ramses Santacruz-Marquez, Marijose Gonzalez-De los Santos, Isabel Hernandez-Ochoa
Summary: The ovary plays a crucial role in female reproduction, but exposure to nanoparticles may disrupt its normal functions. While studies have shown that physicochemical characteristics of nanoparticles affect their effects in non-reproductive tissues or systems, research on nanoparticle-mediated reproductive toxicity overlooks this aspect.
REPRODUCTIVE TOXICOLOGY
(2021)
Article
Toxicology
Estela J. Jauregui, Jasmine Lock, Lindsay Rasmussen, Zelieann R. Craig
Summary: Exposure to di-n-butyl phthalate (DBP) in mice has reproductive toxicity, with its metabolite mono-n-butyl phthalate (MBP) detected in the ovaries and livers, altering the expression of biotransformation enzymes.
TOXICOLOGICAL SCIENCES
(2021)
Article
Toxicology
Angel Ramos-Flores, Israel Camacho-Hernandez, Adolfo Sierra-Santoyo, Maria de Jesus Solis-Heredia, Francisco Alberto Verdin-Betancourt, Lyda Yuliana Parra-Forero, Ma de Lourdes Lopez-Gonzalez, Isabel Hernandez-Ochoa, Betzabet Quintanilla-Vega
Summary: Research on the effects of temephos in male rats found that the pesticide inhibits AChE activity, decreases sperm motility and viability, increases lipoperoxidation, and is metabolized in reproductive tissues. These findings suggest that temephos poses risks to reproductive health at recommended safe concentrations, highlighting the need for a reevaluation of its toxicity.
TOXICOLOGICAL SCIENCES
(2021)
Article
Nutrition & Dietetics
Jorge Briseno-Bugarin, Isabel Hernandez-Ochoa, Xelha Araujo-Padilla, Maria Angelica Mojica-Villegas, Ricardo Ivan Montano-Gonzalez, Gabriela Gutierrez-Salmean, German Chamorro-Cevallos
Summary: Our study demonstrated that PBP crude extract at 200 mg/kg mitigated oxidative damage in the testes and ameliorated alterations in sperm parameters in mice treated with CP, indicating the potential of PBP extract as a protective agent against CP toxicity.
Article
Environmental Sciences
Karen Chiu, Shah Tauseef Bashir, Liying Gao, Jessica Gutierrez, Maria R. C. de Godoy, Jenny Drnevich, Christopher J. Fields, Isaac Cann, Jodi A. Flaws, Romana A. Nowak
Summary: Subacute exposure to DEHP during pregnancy significantly affected the cecal microbiome in pregnant mice, increasing alpha diversity and altering beta diversity. DEHP exposure led to an increase in the relative abundance of Bacteroidetes and a decrease in the relative abundance of Firmicutes and Deferribacteres. In addition, DEHP exposure significantly altered 14 functional pathways, but did not have a significant impact on fertility and birth outcomes.
Article
Environmental Sciences
Karen K. Chiu, Shah Tauseef Bashir, Ahmed M. Abdel-Hamid, Lindsay V. Clark, Mary J. Laws, Isaac Cann, Romana A. Nowak, Jodi A. Flaws
Summary: This study examines the impact of DiNP exposure on the gut microbiota and immune-related factors, revealing that DiNP exposure can alter the composition of the gut microbiota and that the gut contains microbes capable of degrading DiNP.
Article
Environmental Sciences
Diana C. Pacyga, Brad A. Ryva, Romana A. Nowak, Serdar E. Bulun, Ping Yin, Zhong Li, Jodi A. Flaws, Rita S. Strakovsky
Summary: This study investigated the association between phthalates and fibroids and found that each two-fold increase in sigma DEHP or sigma AA was associated with a 13% and 16% greater risk of prior fibroid diagnosis in women. These associations were strongest in women who were overweight/obese and in those diagnosed with fibroids within 5 years before phthalate assessment.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Review
Environmental Sciences
Endia J. Fletcher, Ramses Santacruz-Marquez, Vasiliki E. Mourikes, Alison M. Neff, Mary J. Laws, Jodi A. Flaws
Summary: This review discusses the effects of phthalate mixtures on the female reproductive system. Phthalates have been found to target the ovaries, disrupting normal reproductive function and potentially affecting folliculogenesis and steroidogenesis. Additionally, phthalates can cross the placental barrier and impact fetal development. However, the specific effects of phthalate mixtures on the ovary and female reproduction are still not well understood.
Editorial Material
Reproductive Biology
Zelieann R. Craig, Aileen F. Keating
REPRODUCTIVE TOXICOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Nitya Shree, Zehuan Ding, Jodi Flaws, Mahua Choudhury
Summary: The prevalence of poor metabolic health is increasing exponentially worldwide, with exposure to environmental chemicals, such as endocrine-disrupting chemicals (EDCs), gaining attention as a contributing factor. Recent reports suggest that non-coding RNAs, specifically microRNAs (miRs), may play a vital role in this phenomenon.
Review
Environmental Sciences
Xing Zhang, Jodi A. Flaws, Michael J. Spinella, Joseph Irudayaraj
Summary: Endocrine disrupting chemicals (EDCs) are exogenous substances that can alter the endocrine function of an organism, leading to adverse effects on growth, development, metabolism, and reproductive function. Studies have shown that EDCs can cause proteinuria, affect glomeruli and renal tubules, and even lead to diabetes and renal fibrosis in both animals and humans. This review discusses the renal accumulation of specific EDCs, such as dioxins, per- and polyfluoroalkyl substances (PFAS), bisphenol A (BPA), and phthalates, and explores how exposure to these EDCs can result in renal lesions and diseases, including cancer. The regulation of target genes and activation of related pathways by typical EDCs are also summarized.
Article
Environmental Sciences
Vasiliki E. Mourikes, Ramses Santacruz Marquez, Ashley Deviney, Alison M. Neff, Mary J. Laws, Jodi A. Flaws
Summary: Imidacloprid is a neonicotinoid pesticide used in agriculture, gardening, and veterinary medicine. It has the potential to accumulate in the environment and harm non-targeted species. Imidacloprid and its metabolite desnitro-imidacloprid have different effects on follicle growth and steroidogenesis. These compounds also alter the expression of genes related to hormone regulation and apoptosis.
Article
Environmental Sciences
Ramses Santacruz-Marquez, Jodi A. Flaws, Luz del Carmen Sanchez-Pena, Isabel Hernandez-Ochoa
Summary: The use of zinc oxide nanoparticles may potentially affect the functions of ovarian antral follicles, leading to increased estrogen production and an antioxidant response.
Article
Genetics & Heredity
Clotilde Maurice, Mathieu Dalvai, Romain Lambrot, Astrid Deschenes, Marie-Pier Scott-Boyer, Serge McGraw, Donovan Chan, Nancy Cote, Ayelet Ziv-Gal, Jodi A. Flaws, Arnaud Droit, Jacquetta Trasler, Sarah Kimmins, Janice L. Bailey
Summary: The study found that paternal exposure to POPs pollutants can impact the development and pregnancy outcomes of offspring, with this effect potentially explained by sperm DNA methylation. Experimental results suggest that the paternal methylome is sensitive to the environment, with some perturbations persisting for at least two subsequent generations.
