Article
Biology
Qiuying Liu, Xiaoli Chen, Mariah K. Novak, Shaojie Zhang, Wenqian Hu
Summary: Research shows that Trim71 represses Ago2 mRNA translation in mouse embryonic stem cells, which leads to an increase in mature let-7 microRNAs and affects stemness and differentiation. This highlights the importance of repressing the prodifferentiation let-7 microRNAs for maintaining pluripotency.
Article
Biochemistry & Molecular Biology
Chun Li, Bohyung Yoon, Giovanni Stefani, Frank J. Slack
Summary: This study reveals a novel role for lipid kinase PPK-1/PIP5K1A in regulating miRNA levels. PPK-1/PIP5K1A is able to control the levels of let-7 miRNA in C. elegans and human cells. It interacts with nuclear export protein XPO5 to block the binding of XPO5 to pre-let-7 miRNA, thus regulating the levels of mature miRNA.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biology
Ruhi Patel, Himani Galagali, John K. Kim, Alison R. Frand
Summary: This study investigates the regulation mechanism of the molting cycle in Caenorhabditis elegans. The researchers discovered a negative feedback loop between NHR-23 and the let-7 family of microRNAs, which regulates the frequency and number of molts. This feedback loop not only affects the molting cycle, but also regulates other genes involved in molting. The results suggest that this feedback mechanism may be conserved across different species.
Article
Entomology
Dan-Yan Huang, Xiao-Ling Xia, Run Huang, Sheng Li, Dong-Wei Yuan, Su-Ning Liu
Summary: The study reveals a novel role of 20E-induced let-7 in controlling growth during larval-prepupal transition in insects, by modulating cell size and endoreduplication in the fat body, which is critical for larval growth.
Review
Biochemistry & Molecular Biology
David L. Bernstein, Xinpei Jiang, Slava Rom
Summary: The let-7 family of microRNAs is highly expressed in the cerebral and cardiovascular systems, and plays a role in cardiovascular diseases and associated inflammation. It is involved in the development and differentiation of embryonic stem cells in the cardiovascular system, and may serve as a research focus for potential therapeutic targets in cardiovascular diseases.
Article
Cell Biology
Dahai Wang, Mayuri Tanaka-Yano, Eleanor Meader, Melissa A. Kinney, Vivian Morris, Edroaldo Lummertz da Rocha, Nan Liu, Tianxin Liu, Qian Zhu, Stuart H. Orkin, Trista E. North, George Q. Daley, R. Grant Rowe
Summary: Hematopoiesis changes over life to meet the demands of maturation and aging. The study reveals that the remodeling of the definitive hematopoietic stem and progenitor cell (HSPC) compartment is regulated by the Lin28b/let-7 axis, and the Cbx2 gene plays a crucial role in this process.
Article
Genetics & Heredity
Charles Nelson, Victor Ambros
Summary: The let-7 gene encodes a highly conserved microRNA crucial for cell fate specification and developmental progression in animals. Some nematode species within the Caenorhabditis genus lack let-7 gene, but they possess let-7-family microRNAs that target LIN-41 mRNA.
G3-GENES GENOMES GENETICS
(2021)
Article
Cell Biology
Min Gong, Min Wang, Jie Xu, Bin Yu, Yi-Gang Wang, Min Liu, Muhammad Ashraf, Meifeng Xu
Summary: The genetically modified MSCs with GATA-4 overexpression released EVs that had an increased capacity for promoting angiogenesis, likely due to the delivery of let-7 miRs targeting THBS1 in endothelial cells.
Article
Multidisciplinary Sciences
Alexandria C. Wells, Kaito A. Hioki, Constance C. Angelou, Adam C. Lynch, Xueting Liang, Daniel J. Ryan, Iris Thesmar, Saule Zhanybekova, Saulius Zuklys, Jacob Ullom, Agnes Cheong, Jesse Mager, Georg A. Hollander, Elena L. Pobezinskaya, Leonid A. Pobezinsky
Summary: The success of CD8 T cell immune response relies on the formation of memory cells and protection from exhaustion. Let-7 miRNAs play a crucial role in CD8 T cell fate, where maintaining let-7 expression leads to memory CD8 T cell formation and tumor clearance, while let-7 deficiency promotes the generation of exhausted terminal effector cells that fail to reject tumors. Mechanistically, let-7 restrains metabolic changes during T cell activation by inhibiting the PI3K/AKT/mTOR signaling pathway and production of reactive oxygen species. This study suggests that preserving the multipotency of effector cells, rather than enhancing differentiation efficacy, could be a strategy for developing next-generation immunotherapies.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Miguel Quevillon Huberdeau, Vivek Nilesh Shah, Smita Nahar, Julia Neumeier, Francois Houle, Astrid Bruckmann, Foivos Gypas, Kotaro Nakanishi, Helge Grosshans, Gunter Meister, Martin J. Simard
Summary: Argonaute proteins are essential for microRNA-mediated gene silencing in animals. In C. elegans, phosphorylation of ALG-1 at serine 642 regulates microRNA binding, affecting development and viability. Protein kinase A (PKA) KIN-1 is identified as a potential kinase for phosphorylating ALG-1 at serine 642.
Article
Biology
Andrei Marian Niculae, Maria Dobre, Vlad Herlea, Teodora Ecaterina Manuc, Bogdan Trandafir, Elena Milanesi, Mihail Eugen Hinescu
Summary: Increased activity of the insulin-like growth factor (IGF) axis is associated with the development and progression of colorectal cancer (CRC). The let-7 family of microRNAs (miRNAs) has been shown to target genes involved in the IGF signaling pathway and function as tumor suppressors. This study found that multiple let-7 miRNAs were down-regulated in CRC tissues, and that specific dysregulation of let-7 miRNAs was observed in CRC with perineural invasion. These findings suggest a relationship between the IGF axis, let-7 miRNAs, and CRC, as well as an association between the expression of these miRNAs and perineural invasion.
Article
Entomology
Yu Fu, Yi Wang, Qunxia Huang, Chenyue Zhao, Xinmei Li, Yunchao Kan, Dandan Li
Summary: This study found that lncRNA lncR17454 in silkworm is involved in the metamorphosis process through the let-7 miRNA cluster and the ecdysone signaling pathway, providing new clues for lepidopteran pest control.
JOURNAL OF INSECT SCIENCE
(2022)
Article
Cell Biology
Asghar Ali, Muhammad A. Iqbal, Muhammad W. Abbas, Gerrit J. Bouma, Russell Anthony, Thomas E. Spencer, Quinton A. Winger
Summary: In this study, transcriptome analysis revealed the potential roles of the LIN28-let-7 axis in placental and fetal development in ruminants, beyond its involvement in trophoblast proliferation and conceptus elongation.
Review
Cell Biology
Yuxi Ma, Na Shen, Max S. Wicha, Ming Luo
Summary: Cancer stem-like cells play a critical role in tumorigenesis, cancer progression, treatment resistance, and metastatic relapse. The let-7 family of microRNAs is important in regulating cancer stemness through various RNA-binding proteins, long non-coding RNAs, and circular RNAs. Targeting let-7 for cancer therapeutics has the potential to improve treatment efficacy by specifically targeting CSCs and modulating metabolic pathways.
Article
Biochemistry & Molecular Biology
Seong Heon Kim, Bi-Oh Park, Kidae Kim, Byoung Chul Park, Sung Goo Park, Jeong-Hoon Kim, Sunhong Kim
Summary: This study revealed that Sjogren Syndrome antigen B (SSB) protein interacts with LIN28A and LIN28B in the nucleus, regulating the LIN28-let-7 axis and impacting developmental timing control.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Article
Biochemistry & Molecular Biology
Mingxue Cui, Max L. Cohen, Cindy Teng, Min Han
Article
Urology & Nephrology
Pavan K. Bhatraju, Max Cohen, Ryan J. Nagao, Eric D. Morrell, Susanna Kosamo, Xin-Ya Chai, Robin Nance, Victoria Dmyterko, Joseph Delaney, Jason D. Christie, Kathleen D. Liu, Carmen Mikacenic, Sina A. Gharib, W. Conrad Liles, Ying Zheng, David C. Christiani, Jonathan Himmelfarb, Mark M. Wurfel
Editorial Material
Respiratory System
Ying Wei, Wenting Dong, Julia Jackson, Tsung-Che Ho, Claude Jourdan Le Saux, Alexis Brumwell, Xiaopeng Li, Julia Klesney-Tait, Max L. Cohen, Paul J. Wolters, Harold A. Chapman
Summary: The study found that EGCG can not only inhibit TGF beta 1 signaling and new collagen accumulation, but also activate matrix metalloproteinase-dependent collagen I turnover, suggesting the potential for slow fibrosis resolution with continued treatment.
Article
Cell Biology
Jaymin J. Kathiriya, Chaoqun Wang, Minqi Zhou, Alexis Brumwell, Monica Cassandras, Claude Jourdan Le Saux, Max Cohen, Kostantinos-Dionysios Alysandratos, Bruce Wang, Paul Wolters, Michael Matthay, Darrell N. Kotton, Harold A. Chapman, Tien Peng
Summary: The study reveals that human alveolar type 2 cells can transdifferentiate into basal cells in response to fibrotic signaling, driving the pathology associated with severe lung injuries. Aberrant mesenchyme plays a crucial role in the lineage trajectory leading to the expansion of alveolar metaplastic basal cells.
NATURE CELL BIOLOGY
(2022)