期刊
TOXICOLOGY AND APPLIED PHARMACOLOGY
卷 236, 期 2, 页码 202-209出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.taap.2009.01.023
关键词
Arsenic; Endothelial cells; Heme oxygenase 1; Cytokine; Monocyte adhesion
资金
- NHLBI NIH HHS [HL091280, R21 HL091280] Funding Source: Medline
- NIGMS NIH HHS [R15 GM081811, GM081811] Funding Source: Medline
Heme oxygenase-1 (HO-1) is an oxidative stress responsive gene upregulated by various physiological and exogenous stimuli. Arsenite, as an oxidative stressor, is a potent inducer of HO-1 in human and rodent cells. In this study, we investigated the mechanistic role of arsenite-induced HO-1 in modulating tumor necrosis Factor alpha (TNF-alpha) induced monocyte adhesion to human umbilical vein endothelial cells (HUVEC). Arsenite pretreatment, which upregulated HO-1 in a time and concentration-dependent manner, inhibited TNF-alpha-induced monocyte adhesion to HUVEC and intercellular adhesion molecule 1 protein expression by 50% and 40%, respectively importantly, knockdown of HO-1 by small interfering RNA abolished the arsenite-induced inhibitory effects. These results indicate that induction of HO-1 by arsenite inhibits the cytokine-induced monocyte adhesion to HUVEC by suppressing adhesion molecule expression. These Findings established in important mechanistic link between the functional monocyte adhesion properties of HUVEC and the induction of HO-1 by arsenite. (C) 2009 Elsevier Inc. All rights reserved
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