期刊
TOXICOLOGY AND APPLIED PHARMACOLOGY
卷 230, 期 3, 页码 352-357出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.taap.2008.03.008
关键词
fluoride; in vitro fertilization (IVF); spermatozoa; oxidative stress; mitochondrial transmembrane potential
Fluorosis, caused by drinking water contamination with inorganic fluoride, is a public health problem in many areas around the world. The aim of the study was to evaluate the effect of environmentally relevant doses of fluoride on in vitro fertilization (IVF) capacity of spermatozoa, and its relationship to spermatozoa mitochondrial transmembrane potential (Delta Psi(m)). Male Wistar rats were administered at 5 mg fluoride/kg body mass/24 h, OF deionized water orally for 8 weeks. We evaluated several spermatozoa parameters in treated and untreated rats: i) standard quality analysis, ii) superoxide dismutase (SOD) activity, iii) the generation of superoxide anion (O-2(center dot-)), iV) lipid peroxidation concentration, v) ultrastructural analyses of spermatozoa using transmission electron Microscopy, vi) Delta Psi(m), vii) acrosome reaction, and viii) IVF capability. Spermatozoa from fluoride-treated rats exhibited a significant decrease in SOD activity (similar to 33%), accompanied with a significant increase in the generation of O-2 (similar to 40%), a significant decrease in Delta Psi(m) (similar to 33%), and a significant increase in lipid peroxidation concentration (similar to 50%), relative to spermatozoa from the control group. Consistent with this finding, spermatozoa from fluoride-treated rats exhibited altered plasmatic membrane. In addition, the percentage of fluoride-treated spermatozoa capable of undergoing the acrosome reaction was decreased relative to control spermatozoa (34 vs. 55%), while the percentage fluoride-treated spermatozoa capable of oocyte fertilization was also significantly lower than the control group (13 vs. 71%). These observations suggest that subchronic exposure to fluoride causes oxidative stress damage and loss of mitochondrial transmembrane potential, resulting in reduced fertility. (C) 2008 Elsevier Inc. All rights reserved.
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