期刊
TOXICOLOGY
卷 293, 期 1-3, 页码 107-114出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2012.01.008
关键词
Diclofenac; Drug-induced liver injury; Inflammation; IL-1 beta; IL-17
资金
- Grants-in-Aid for Scientific Research [21590433] Funding Source: KAKEN
Drug-induced liver injury (DILI) is a major safety concern in drug development and clinical drug therapy. However, the underlying mechanism of DILI is little known. It is difficult to predict DILI in humans due to the lack of experimental animal models. Diclofenac, a non-steroidal anti-inflammatory drug rarely causes severe liver injury in human, but there is some evidence for immunoallergic idiosyncratic reactions. In this study, the mechanism of diclofenac-induced liver injury in mice was investigated. First, we established the dosing condition for liver injury in normal mice. Plasma ALT and AST levels were significantly increased in diclofenac-administered (80 mg/kg, i.p.) mice in a dose- and time-dependent manner. Among several interleukins (ILs) and chemokines, mRNA expression of helper T (Th) 17 cell-mediated factors, such as retinoid orphan receptor (ROR)-gamma t, and signal transducers and activators of transcription factor (STAT) 3 in the liver, and the plasma IL-17 level were significantly increased. Neutralization of IL-17 tended to suppress the hepatotoxicity of diclofenac, suggesting that IL-17 was partly involved. Gadolinium chloride (GdCl3) administration demonstrated that Kupffer cells are not likely to be involved in diclofenac hepatotoxicity. Hepatic expressions of IL-1 beta mRNA and plasma IL-1 beta were significantly increased soon after the diclofenac administration. Then, the results of an in vivo neutralization study of IL-1 beta suggested that IL-1 beta was involved early in the time of pathogenesis of the diclofenac-induced liver injury. In conclusion, we firstly developed a diclofenac-induced acute liver injury model in normal mice, and the involvement of IL-17 and IL-1 beta was clarified. (c) 2012 Elsevier Ireland Ltd. All rights reserved.
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