Article
Medicine, Research & Experimental
Fabio Alejandro Aguilar Mora, Nshunge Musheshe, Johanna C. Arroyave Ospina, Yana Geng, Juan M. Soto, Jose A. Rodrigo, Tatiana Alieva, Manon Buist-Homan, Frank Lezoualc'h, Xiaodong Cheng, Martina Schmidt, Han Moshage
Summary: The study demonstrates that metformin protects against diclofenac (DF)-induced apoptosis in hepatocytes, with this protective effect being dependent on EPAC-2. Metformin is able to reverse the mitochondrial dysfunction induced by DF.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Yasuhiro Umemura, Nobuya Koike, Yoshiki Tsuchiya, Hitomi Watanabe, Gen Kondoh, Ryoichiro Kageyama, Kazuhiro Yagita
Summary: Circadian clocks are suppressed during early embryonic stages and pluripotent stem cells in mammals, but gradually emerge during ontogenesis. The expression of CLOCK/BMAL1 affects the oscillation of the segmentation clock, which controls somitogenesis in the early developmental stage.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Konrad A. Szychowski, Bartosz Skora, Anna Kryshchyshyn-Dylevych, Danylo Kaminskyy, Jakub Tobiasz, Roman B. Lesyk, Jan Gminski
Summary: The study found that compounds like Les-2194, Les-3377, and Les-3640 have cytotoxic effects on 3T3-L1 cells, but they can also stimulate lipid accumulation and induce specific gene expression. Les-3377 negatively impacted the number and metabolism of cells.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Environmental Sciences
Ruirui Li, Bin Liu, Wenping Xu, Lvnan Yu, Cheng Zhang, Jiagao Cheng, Liming Tao, Zhong Li, Yang Zhang
Summary: The study revealed the cytotoxic effect of fungicide DFC on mouse monocyte macrophage RAW264.7, including inhibiting cell viability, inducing DNA damage, and enhancing apoptosis.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Luise Fuhr, Alireza Basti, Teresa Silva Bras, Maria F. Duarte, Angela Relogio
Summary: This study investigated the treatment effect of C. cardunculus on human colorectal cancer cells and found that it directly affects the circadian rhythm of the cells, inhibiting proliferation and inducing cytotoxicity and apoptosis. These findings suggest that C. cardunculus may have a potential role as a modulator of the circadian clock.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Health Care Sciences & Services
Betul Cicek, Sidika Genc, Yesim Yeni, Mehmet Kuzucu, Ahmet Cetin, Serkan Yildirim, Ismail Bolat, Mecit Kantarci, Ahmet Hacimuftuoglu, Georgios Lazopoulos, Aristidis Tsatsakis, Konstantinos Tsarouhas, Ali Taghizadehghalehjoughi
Summary: This study aimed to investigate the beneficial effects of artichoke leaf extract on DEN-induced brain toxicity. The results showed that high-dose artichoke treatment effectively improved impaired neuronal function and oxidative stress induced by DEN, and significantly increased the levels of neuroprotective factors Klotho and PPAR gamma.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Multidisciplinary Sciences
Charatda Punvittayagul, Theerapat Luangsuphabool, Rawiwan Wongpoomchai
Summary: The study found that protocatechuic acid (PCA) is an active compound in the anticarcinogenic action of purple rice bran extract. PCA inhibits the formation of glutathione S-transferase-positive foci and has anti-inflammatory effects. In the promotion stage, PCA suppresses cell proliferation and induces apoptosis, thereby exerting its anticancer effects.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Fauzia Jamal, Ghufran Ahmed, Mohammad Farazuddin, Ishrat Altaf, Saba Farheen, Qamar Zia, Asim Azhar, Hira Ahmad, Aijaz Ahmed Khan, Satyanarayana Somavarapu, Anshu Agrawal, Mohammad Owais
Summary: Nanotechnology-based subtilosomes encapsulating COX-2-specific siRNA showed promising results in inhibiting DEN-induced hepatocellular carcinoma, with sustained release and pH-responsive features.
Article
Biochemistry & Molecular Biology
Soo-Jin Lee, Sung-E Choi, Seokho Park, Yoonjung Hwang, Youngho Son, Yup Kang
Summary: This study found that high fructose concentrations induced mitochondria mediated apoptotic cell death in mouse primary hepatocytes, and endoplasmic reticulum stress responses were involved. In addition, inhibitors of AMPK activation and PPAR-α activation protected against fructose-induced death, while a CPT I inhibitor exacerbated the toxicity.
MOLECULES AND CELLS
(2023)
Article
Biochemistry & Molecular Biology
Yuka Takahashi, Chikara Abe, Masaya Hane, Di Wu, Ken Kitajima, Chihiro Sato
Summary: Schizophrenia is a severe psychiatric disorder influenced by genetic and environmental factors. Changes in polysialylation of neural cell adhesion molecules have been found in the brains of patients with psychiatric disorders. This study investigates the effects of genetic (G) and environmental (E) factors on polysialylated NCAM expression using a schizophrenia mouse model and acute stress conditions. The study discovers differences in polysialylation and NCAM responses to stress in different brain regions, suggesting the vulnerability of the schizophrenia mouse model to stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Zhaoliang Peng, Jiaying Wu, Shijun Hu, Aijin Ma, Lei Wang, Nan Cao, Yu Zhang, Qiyuan Li, Junying Yu, Shufang Meng, Tao Na, Xiaolei Shi, Mei Li, Huadong Liu, Linguang Qian, E. Tian, Feng Lin, Jiani Cao, Yaojin Peng, Huanxin Zhu, Lingmin Liang, Jie Hao, Tongbiao Zhao, Xin Cheng, Guoyu Pan
Summary: The 'Requirements for Primary Human Hepatocyte' is the first set of guidelines in China that specifies technical requirements, test methods, regulations, and storage for Primary Human Hepatocyte. These guidelines were originally released by the China Society for Cell Biology on January 9, 2021, with the aim of promoting institutional establishment, acceptance, and execution of proper protocols and accelerating international standardization of Primary Human Hepatocyte applications.
CELL PROLIFERATION
(2022)
Article
Food Science & Technology
Gloria Stephanie Villa-Jaimes, Fabio Alejandro Aguilar-Mora, Herson Antonio Gonzalez-Ponce, Francisco Javier Avelar-Gonzalez, Ma Consolacion Martinez Saldana, Manon Buist-Homan, Han Moshage
Summary: The study found that Opuntia spp-extracts can effectively protect against liver toxicity induced by diclofenac, reducing oxidative stress and inhibiting P53 to protect the liver.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Developmental Biology
Dawid Winiarczyk, Anna Piliszek, Silvestre Sampino, Marek Lukaszewicz, Jacek Andrzej Modlinski
Summary: Programmed cell death, also known as apoptosis, is crucial in mammalian development, with the occurrence influenced by factors such as cell number, nucleus to cytoplasm ratio, and cell volume. Manipulating these factors in 2-cell embryos can lead to subtle changes in apoptosis frequency, with a higher probability of apoptosis observed in certain embryo structures. The stage of embryo development and cell lineage have the greatest impact on the likelihood of apoptosis.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2021)
Article
Oncology
Judit Domenech Omella, Emanuela E. Cortesi, Iris Verbinnen, Michiel Remmerie, Hanghang Wu, Francisco J. Cubero, Tania Roskams, Veerle Janssens
Summary: The deletion of Ppp2r5d gene accelerates the development of hepatocellular carcinoma (HCC) in a mouse model of DEN-induced hepatocarcinogenesis, suggesting its tumor-suppressive function. The deletion of Ppp2r5d also leads to the development of HCC and combined hepatocellular-cholangiocarcinoma (cHCC-CCA), and may regulate its own expression to restrict tumor growth.
Article
Biotechnology & Applied Microbiology
Shanshan Li, Yang Li, Hongjian Sun, Yang Jiang, Keming Pan, Yue Su, Nan Bu
Summary: This study demonstrated that Mulberry fruit polysaccharides (MFP) exhibit chemopreventive effects against DEN/PB-induced hepatocarcinogenesis by inhibiting inflammation and inducing apoptosis.
Article
Chemistry, Medicinal
Mami Sato, Hirokazu Fuchida, Naoya Shindo, Keiko Kuwata, Keisuke Tokunaga, Guo Xiao-Lin, Ryo Inamori, Keitaro Hosokawa, Kosuke Watari, Tomohiro Shibata, Naoya Matsunaga, Satoru Koyanagi, Shigehiro Ohdo, Mayumi Ono, Akio Ojida
ACS MEDICINAL CHEMISTRY LETTERS
(2020)
Article
Multidisciplinary Sciences
Mizuki Kato, Yuya Tsurudome, Takumi Kanemitsu, Sai Yasukochi, Yuki Kanado, Takashi Ogino, Naoya Matsunaga, Satoru Koyanagi, Shigehiro Ohdo
SCIENTIFIC REPORTS
(2020)
Article
Pharmacology & Pharmacy
Sai Yasukochi, Naoki Kusunose, Naoya Matsunaga, Satoru Koyanagi, Shigehiro Ohdo
Summary: Diurnal variations in pain hypersensitivity are common in chronic pain disorders, with exacerbation of neuropathic pain hypersensitivity depending on glucocorticoid secretion. The study found that the drug SSZ has inhibitory effects on SGK-1 in the spinal cord, and concomitant administration with FBX can enhance its anti-neuropathic pain effects.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yuji Omata, Tomoaki Yamauchi, Akito Tsuruta, Naoya Matsunaga, Satoru Koyanagi, Shigehiro Ohdo
Summary: The study revealed that ADAR1-mediated regulation of alternative splicing of the ABCB1 gene is a key mechanism for the circadian expression of P-glycoprotein (P-gp) in human renal proximal tubular epithelial cells (RPTECs). This regulatory mechanism may explain the dosing time-dependent variations in the renal elimination of P-gp substrates.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Yuya Yoshida, Naoya Matsunaga, Takaharu Nakao, Kengo Hamamura, Hideaki Kondo, Tomomi Ide, Hiroyuki Tsutsui, Akito Tsuruta, Masayuki Kurogi, Michio Nakaya, Hitoshi Kurose, Satoru Koyanagi, Shigehiro Ohdo
Summary: Dysfunction of the circadian clock is linked to cardiovascular disease pathogenesis. Even in the presence of high blood pressure and elevated levels of angiotensin II, Clk/Clk mice with chronic kidney disease show reduced cardiac inflammation and fibrosis. The monocytic expression of GPR68, induced by altered CLOCK activation, exacerbates heart inflammation and fibrosis under CKD conditions.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Takashi Ogino, Naoya Matsunaga, Takahiro Tanaka, Tomohito Tanihara, Hideki Terajima, Hikari Yoshitane, Yoshitaka Fukada, Akito Tsuruta, Satoru Koyanagi, Shigehiro Ohdo
Summary: The disruption of circadian clock machinery in cancer cells is associated with tumor malignancy. High ALDH activity in breast cancer stem-like cells is negatively regulated by the major circadian component CLOCK. The inhibition of CLOCK expression by miR-182 affects the stemness properties of breast cancer cells, suggesting a novel strategy for breast cancer treatment.
Correction
Pharmacology & Pharmacy
Sai Yasukochi, Naoki Kusunose, Naoya Matsunaga, Satoru Koyanagi, Shigehiro Ohdo
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Oncology
Akito Tsuruta, Yuki Shiiba, Naoya Matsunaga, Marina Fujimoto, Yuya Yoshida, Satoru Koyanagi, Shigehiro Ohdo
Summary: Cancer cells have developed ways to evade the host immune system, including through the PD-1/PD-L1 immune checkpoint pathway. This study found that the expression of PD-1 on tumor-associated macrophages (TAMs) fluctuates in a diurnal manner and is regulated by a circadian regulatory component called DEC2. Furthermore, administering PD-1/PD-L1 inhibitors at the time of day when PD-1 expression on TAMs is increased enhances their anti-tumor efficacy.
MOLECULAR CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yuji Omata, Maseri Okawa, Mai Haraguchi, Akito Tsuruta, Naoya Matsunaga, Satoru Koyanagi, Shigehiro Ohdo
Summary: This study reveals that downregulation of ADAR1 increases the expression of MRP4 in human renal cells. ADAR1 represses the biogenesis of circRNA circHIPK3 through its RNA editing activity, which interferes with miR-381-3p and induces the expression of MRP4.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Allergy
Shigehiro Ohdo, Satoru Koyanagi, Naoya Matsunaga
Summary: Clock genes regulate circadian rhythms in physiology and behavior, and play a role in various diseases. The timing of drug administration is crucial for its effectiveness and safety. Immune checkpoint therapy for cancer and treatment for chronic kidney disease require consideration of dosing time.
ALLERGOLOGY INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Ken-ichi Hashikawa, Akito Tsuruta, Wakaba Yamakawa, Sai Yasukochi, Satoru Koyanagi, Shigehiro Ohdo
Summary: Renewal of retinal photoreceptor outer segments involves shedding and phagocytosis, but senescence can modulate the circadian phagocytic activity of RPE cells. The phagocytic activity of senescent ARPE-19 cells increased constantly and exhibited altered circadian oscillation, accompanied by changes in the expression of circadian clock genes. Additionally, activation of the circadian clock component REV-ERBa enhanced the phagocytic activity of normal ARPE-19 cells and increased the expression of phagocytosis-related genes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Kohei Fukuoka, Yuya Yoshida, Kurumi Sotono, Naoki Nishikawa, Kengo Hamamura, Kosuke Oyama, Akito Tsuruta, Kota Mayanagi, Satoru Koyanagi, Naoya Matsunaga, Shigehiro Ohdo
Summary: Chronic kidney disease (CKD) disrupts the intestinal microbiota, leading to cardiovascular inflammation and fibrosis. In this study, a mouse model was used to explore the effects of administering vancomycin (VCM) on the microbiota and cardiac pathology associated with CKD. Despite advanced renal dysfunction, VCM-induced changes in the intestinal microbiota composition reduced cardiotoxic substances, alleviated heart failure, and improved survival rates. These findings highlight the potential of targeting the intestinal microbiota to prevent and treat cardiovascular conditions in CKD.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)