期刊
TOXICOLOGY
卷 248, 期 1, 页码 33-38出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2008.03.004
关键词
Otos; spiral ligament fibrocytes; cisplatin; recombinant adenovirus; up-regulation
Previous reports have implicated Otos, a novel specific gene expressed by spiral ligament fibrocytes (SLFs) with unclear functions, as a protective gene for cochlea. However, whether Otos gene could protect SLFs against cisplatin (DDP)-induced apoptosis remains largely unknown. In the present study, we utilized Adenoviral-mediated gene transfection to up-regulate Otos expression in cultured SLFs and further assessed the cell viability and apoptosis as well as possible MAPK and mitochondrial pathways. As expected, the data showed that Otos up-regulation significantly decreased apoptosis of SLFs induced by DDP possibly through activation of ERK and partial inhibition of JNK and mitochondrial pathway but not p-38 pathway, suggesting Otos as a potential protective gene for cochlea and raising the possibility of Otos up-regulation as a promising approach to DDP-induced deafness therapy. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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