Article
Biochemistry & Molecular Biology
Je Lin Sieow, Hweixian Leong Penny, Sin Yee Gun, Ling Qiao Tan, Kaibo Duan, Joe Poh Sheng Yeong, Angela Pang, Diana Lim, Han Chong Toh, Tony Kiat Hon Lim, Edgar Engleman, Olaf Rotzschke, Lai Guan Ng, Jinmao Chen, Suet Mien Tan, Siew Cheng Wong
Summary: A large number of neutrophils infiltrate tumors and contribute to the inflammatory tumor microenvironment. The metabolic status of these neutrophils and their role in tumor progression were investigated in a mouse model of pancreatic ductal adenocarcinoma (PDAC). Tumor-infiltrating neutrophils showed enhanced glycolytic activity compared to neutrophils from the bone marrow and blood of the same mouse. Deletion of hypoxia-inducible factor 1-alpha (HIF-1 alpha) in neutrophils reduced tumor burden and improved survival in the mouse model, indicating a potential target for metabolic modulation in cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Bo Zhang, Yan Chen, Lei Bao, Weibo Luo
Summary: This study demonstrates that hypoxia upregulates the expression of GPT2 in GBM cells through HIF-2 activation. GPT2 is localized in the nucleus and mitochondria and can decrease α-ketoglutarate levels in GBM cells. Inhibition of GPT2 can reduce GBM cell growth and migration, and knockout of GPT2 inhibits GBM tumor growth in mice.
Review
Immunology
Essa M. Sabi, Anuja Singh, Ziyad M. Althafar, Tapan Behl, Aayush Sehgal, Sukhbir Singh, Neelam Sharma, Saurabh Bhatia, Ahmed Al-Harrasi, Hosam M. Alqahtani, Simona Bungau
Summary: Rheumatoid arthritis is a chronic disease that affects millions of people worldwide and is closely associated with factors such as hypoxia and angiogenesis. This review will examine how hypoxia activates molecular pathways and how other pathways involving inflammatory signals develop and sustain synovitis in rheumatoid arthritis.
INFLAMMOPHARMACOLOGY
(2022)
Review
Pharmacology & Pharmacy
Xinguo Jiang, Wen Tian, Dongeon Kim, Alexander S. McQuiston, Ryan Vinh, Stanley G. Rockson, Gregg L. Semenza, Mark R. Nicolls
Summary: Lymphedema is a chronic inflammatory disorder with incomplete understanding of its pathology and lack of approved pharmacological therapy. Studying hypoxia-regulated pathways in lymphedema may lead to new treatment approaches.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Pharmacology & Pharmacy
Baoqi Yu, Xia Wang, Yanting Song, Guomin Xie, Shiyu Jiao, Li Shi, Xuejie Cao, Xinyao Han, Aijuan Qu
Summary: Cardiovascular diseases are the leading cause of death worldwide, and hypoxia-inducible factors (HIFs) play an important role in their pathogenesis. Recent studies have revealed the role of cell-specific HIFs in various cardiovascular diseases, but the potential clinical application of HIF inhibitors in the treatment of cardiovascular diseases is not well understood.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Giorgia F. Camagni, Giovanni Minervini, Silvio C. E. Tosatto
Summary: The Prolyl Hydroxylases (PHDs) are a family of enzymes that regulate cell oxygen-sensing by hydroxylating hypoxia-inducible transcription factors alpha (HIFs-alpha) and promoting their degradation. Hypoxia inhibits PHDs activity, leading to HIFs-alpha stabilization and cell adaptation to low oxygen levels. The different isoforms of PHDs have varying effects on tumor progression. Molecular dynamics simulations, conservation analysis, and binding free energy calculations were used to study the binding properties and substrate affinity of PHD2 with HIF-1 alpha and HIF-2 alpha. The findings suggest that the PHD2 C-terminus may play a role in regulating PHD activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Xiangyun Li, Nathanial K. Berg, Tingting Mills, Kaiying Zhang, Holger K. Eltzschig, Xiaoyi Yuan
Summary: Hypoxia and inflammation frequently coexist in ARDS and chronic lung diseases, with adenosine signaling pathway being a key player in mediating the effects. Therapeutic targeting of adenosine signaling pathway has been discussed as a potential strategy in management of these conditions.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Oncology
Wen-Jun Wang, Chao Ouyang, Bin Yu, Chong Chen, Xiao-Feng Xu, Xiao-Qun Ye
Summary: HIF-2 alpha plays a crucial role in tumorigenesis, cell proliferation, angiogenesis, metastasis, drug resistance, and radiotherapy failure in lung cancer. Targeted therapy against HIF-2 alpha has shown promising potential as a new direction for treating lung cancer in the future.
Article
Physiology
Rebecca Berggren-Nylund, Martin Ryde, Anna Lofdahl, Arturo Ibanez-Fonseca, Monica Karedal, Gunilla Westergren-Thorsson, Ellen Tufvesson, Anna-Karin Larsson-Callerfelt
Summary: This study investigated the effect of hypoxia combined with profibrotic stimuli on human lung epithelial cells and its correlation to pathogenesis. The results showed that hypoxia downregulated genes related to fibrosis, mitochondrial stress, oxidative stress, apoptosis, and inflammation. It also resulted in changes in cell viability and metabolic activity. These findings suggest that hypoxia may play a role in the development of chronic lung disorders.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Cell Biology
Davide Marchi, Fredericus J. M. van Eeden
Summary: Glucocorticoids are essential for regulating physiological processes, but chronic steroid use may lead to limited therapeutic benefits. Understanding how to modulate glucocorticoid receptor activity is crucial for minimizing side effects and improving treatment outcomes.
Review
Immunology
Tineke Vanderhaeghen, Rudi Beyaert, Claude Libert
Summary: Glucocorticoid-induced and hypoxia-induced transcriptional responses play crucial roles in maintaining tissue homeostasis and regulating cellular stress and inflammatory responses. Cells respond rapidly to hypoxia to prevent metabolic shutdown and death through hypoxia inducible factors (HIFs). The crosstalk between the glucocorticoid receptor (GR) and HIFs has been investigated, and the therapeutic effects of GCs on HIF-mediated diseases have been discussed.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Oncology
Yuan-rui Xu, An-long Wang, Ya-qing Li
Summary: Patients with chronic obstructive pulmonary disease (COPD) are at a higher risk of developing lung cancer due to chronic persistent inflammation and hypoxia, regardless of their smoking history. The role of hypoxia-inducible factor 1-alpha (HIF-1 alpha) in the crosstalk between inflammation and hypoxia is discussed, as it plays a crucial role in COPD progression to lung cancer.
FRONTIERS IN ONCOLOGY
(2022)
Review
Cell Biology
Madathilparambil Suresh, Sanjay Balijepalli, Sumeet Solanki, Sinan Aktay, Khushi Choudhary, Yatrik M. Shah, Krishnan Raghavendran
Summary: HIFs are critical transcription factors in the adaptive response to hypoxia and are implicated in the dysregulated immune response to various insults. However, there is a lack of studies investigating the role of HIFs in clinically relevant injury models, especially involving the lungs. This review summarizes the effects of HIF-1 alpha on the vasculature, metabolism, inflammation, and apoptosis in the lungs and discusses its role in direct lung injuries, including lung contusion, acid aspiration, pneumonia, and COVID-19.
Review
Biochemistry & Molecular Biology
Alina Mihaela Stanigut, Camelia Pana, Manuela Enciu, Mariana Deacu, Bogdan Cimpineanu, Liliana Ana Tuta
Summary: Research has found that hypoxia response and hypoxia-inducible factors play critical roles in the pathogenesis of diabetic nephropathy, and HIF stabilizers are considered as novel therapeutic agents for treating anemia in CKD patients and relieving oxidative stress in renal tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Wenpeng Cao, Shan Lei, Zhirui Zeng, Chaolun Xiao, Baofei Sun, Peng Xie, Yumei Li, Daopeng Luo, Wenfeng Yu
Summary: This study identified TRAA2A as an important hypoxia-associated gene in pancreatic cancer, which is associated with tumor progression and metastasis. TRAA2A is regulated by hypoxia-inducible factor HIF1 alpha and promotes the proliferation and motility of pancreatic cancer cells via activation of the AKT pathway. Therefore, TRAA2A may serve as a novel molecular target for investigating the hypoxic response of pancreatic cancer cells.
Review
Immunology
Brandon W. Lewis, Sonika Patial, Yogesh Saini
JOURNAL OF IMMUNOLOGY RESEARCH
(2019)
Article
Immunology
Brandon W. Lewis, Thao Vo, Ishita Choudhary, Allison Kidder, Chandra Bathula, Camille Ehre, Nobuko Wakamatsu, Sonika Patial, Yogesh Saini
JOURNAL OF IMMUNOLOGY
(2020)
Review
Toxicology
Sonika Patial, Yogesh Saini
CRITICAL REVIEWS IN TOXICOLOGY
(2020)
Review
Oncology
Yogesh Saini, Jian Chen, Sonika Patial
Article
Immunology
Ishita Choudhary, Thao Vo, Chandra S. Bathula, Richa Lamichhane, Brandon W. Lewis, Jayme Looper, Samithamby Jeyaseelan, Perry J. Blackshear, Yogesh Saini, Sonika Patial
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Sonika Patial, Brandon W. Lewis, Thao Vo, Ishita Choudhary, Kshitiz Paudel, Yun Mao, Dhruthi Singamsetty, Frank Brombacher, Yogesh Saini
Summary: Research has shown that IL-33, ILCs, and IL4R alpha play important roles in the recruitment of eosinophils during normal postnatal lung development in mice. Myeloid-specific IL4R alpha deficiency results in reduced eosinophil numbers in the airspaces and decreased levels of related cytokines.
SCIENTIFIC REPORTS
(2021)
Article
Pathology
Chandra S. Bathula, Jian Chen, Rahul Kumar, Perry J. Blackshear, Yogesh Saini, Sonika Patial
Summary: The study reveals that inactivation of ZFP36L1 can protect the liver from alcohol-induced hepatic steatosis, injury, and inflammation, possibly by stabilizing Fgf21 mRNA. Modulation of ZFP36L1 may be beneficial in the prevention and treatment of alcoholic liver disease.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Cell Biology
Khadeja-Tul Kubra, Mohammad S. Akhter, Yogesh Saini, Konstantin G. Kousoulas, Nektarios Barabutis
Summary: The study demonstrated that ATF6 activation protects endothelial barrier function by counteracting Cofilin and MLC2 activation, as well as VE-Cadherin phosphorylation induced by LPS. Targeting ATF6 may offer a promising therapeutic strategy for sepsis and ARDS by preserving endothelial barrier integrity.
CELLULAR SIGNALLING
(2022)
Article
Pharmacology & Pharmacy
Richa Lamichhane, Sonika Patial, Yogesh Saini
Summary: Research found that male mice are more susceptible to bleomycin-induced lung fibrosis compared to female mice. These gender-related differences may be associated with the transcriptomic repertoire of myeloid cells.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2022)
Article
Cell Biology
Jian Chen, Sonika Patial, Yogesh Saini
Summary: This study reveals the role of ZFP36L1 in regulating epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma cells by directly modulating ZEB2 mRNA. These findings highlight the significance of ZFP36L1 in the development of hepatocellular cancer.
CELLULAR SIGNALLING
(2022)
Article
Pharmacology & Pharmacy
Rahul Kumar, Yun Mao, Sonika Patial, Yogesh Saini
Summary: This study investigated the effect of different Tamoxifen (TAM) regimens on whole-body gene deletion in mice using Tamoxifen-inducible Cre recombinase. The results demonstrated that a combination of TAM-diet with either TAM-injections or TAM-oral gavage showed the most efficient deletion of genes in the whole body.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Thao Vo, Yogesh Saini
Summary: The Cre-LoxP system is commonly used for recombination of floxed genes, but current macrophage-specific Cre recombinase mouse strains have limitations. This study tested the Mafb-Cre strain, which labels macrophages in various organs. The results show that Mafb-Cre only targets around 40% of alveolar macrophages, regardless of age. Mafb+ macrophages exhibit higher expression of surface markers. Bone marrow-derived macrophages are highly amenable to Cre-LoxP recombination, and Th2 stimulation and ozone exposure promote Mafb-Cre-mediated recombination in alveolar macrophages.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Physiology
Durgesh Nandini Das, Bijesh Puthusseri, Venkadesaperumal Gopu, Venugopal Krishnan, Ashoka Kumar Bhagavath, Sudhir Bolla, Yogesh Saini, Gerald J. Criner, Nathaniel Marchetti, Hua Tang, Nagarjun V. Konduru, Liang Fan, Sreerama Shetty
Summary: In this study, elevated expression of caveolin-1, p53, and PAI-1 was observed in airway and alveolar epithelial cells of COPD patients and CS-induced lung injury mice. Deficiency of p53 and PAI-1 protected mice against CS-induced lung injury. Treatment with the caveolin-1 scaffolding domain peptide CSP7 reduced mucus hypersecretion and improved AT2 cell viability. Induction of PAI-1 expression via caveolin-1 and p53 contributed to mucus cell metaplasia and reduced AT2 cell viability, which was reversed by CSP7 treatment. CSP7 administration attenuated mucus hypersecretion, alveolar injury, and improved lung function in CS-induced lung injury mice.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Article
Biochemical Research Methods
Brandon W. Lewis, Sonika Patial, Yogesh Saini
Summary: This study develops a high-throughput screening platform for the determination of macrophage activation markers. The method can be adapted for biomarker determination in pathological conditions and toxicant/drug safety screening.
METHODS AND PROTOCOLS
(2022)
Article
Physiology
Yogesh Saini, Brandon W. Lewis, Dongfang Yu, Hong Dang, Alessandra Livraghi-Butrico, Fabio Del Piero, Wanda K. O'Neal, Richard C. Boucher
PHYSIOLOGICAL REPORTS
(2018)