Article
Biochemistry & Molecular Biology
Sang-Hoon Yoon, Mi-Rae Bae, Hyeonwoo La, Hyuk Song, Kwonho Hong, Jeong-Tae Do
Summary: This study proposed an efficient method for deriving NSCs from mouse ESCs through a combination of 3D culture for early neural lineage commitment and 2D NSC derivation. The results demonstrated that ESCs could efficiently differentiate into tripotent NSCs through specification in 3D culture followed by 2D culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Chao Sun, Yinhua Dong, Jun Wei, Meng Cai, Dongfang Liang, Yongqing Fu, You Zhou, Yi Sui, Fangda Wu, Roman Mikhaylov, Hanlin Wang, Feifei Fan, Zhihua Xie, Mercedes Stringer, Zhiyong Yang, Zhenlin Wu, Liangfei Tian, Xin Yang
Summary: This study develops a highly effective acoustic stimulator working at -20 MHz to investigate the roles of acousto-mechanical stimuli in the differentiation of hESCs. Our results show that acoustic dose alters the expression of extracellular matrix and physiological activity-related genes, indicating that acoustic stimulation is an important tool in regulating the stem cells' differentiation processes in cell therapy.
ACTA BIOMATERIALIA
(2022)
Article
Cell Biology
Masahiro Otsu, Zubair Ahmed, Daniel Fulton
Summary: A simple method was developed to generate immature oligodendrocyte lineage cells from mouse ESC-derived neural stem cells, which showed potential for differentiation into mature OPC under specific conditions. Interestingly, these cells retained multipotentiality and could differentiate into neurons or astrocytes. However, their efficiency in myelinating axons was lower compared to primary OPC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Cell & Tissue Engineering
Kyung-Joo Seong, Seungho Choi, Hyun-Gwan Lee, Joon Haeng Rhee, Jin Ho Lee, Jeong-Tae Koh, Sun-Hun Kim, Won-Seok Choi, Ji-Yeon Jung, Won-Jae Kim
Summary: Toll-like receptor 5 plays a crucial role in regulating neurogenesis from mouse embryonic stem cells and adult hippocampal neural stem cells, promoting neural differentiation and inhibiting cell proliferation. TLR5 deficiency impairs fear memory performance in mice.
Article
Biochemistry & Molecular Biology
Ye Rim Kim, Si Won Jang, Jae Ho Han, Ga Rim Na, Hoon Jang, Hyun Woo Choi
Summary: Research on the technology for in vitro differentiation of embryonic stem cells (ESCs) into neural lineages is crucial for developmental biology, regenerative medicine, and cell therapy. Co-culturing ESCs with neural stem cells (NSCs) promotes ESC differentiation into ectoderm, with higher expression levels of Pax6 and Sox1 observed. NSC-derived conditioned medium (CM) showed lower differentiation efficiency compared to non-conditioned medium.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Seon-Ung Hwang, Kiyoung Eun, Mirae Kim, Junchul David Yoon, Lian Cai, Hyerin Choi, Dongjin Oh, Gabsang Lee, Hyunggee Kim, Eunhye Kim, Sang-Hwan Hyun
Summary: This study demonstrates successful establishment of neuro-organoids derived from porcine embryonic stem-like cells in vitro. The protocol developed in this study can serve as a tool for drug development, patient-specific chemotherapy, and human central nervous system disease studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yan Li, Zhiyu Xia, Haihong Yin, Youran Dai, Feixue Li, Jianming Chen, Mengsheng Qiu, Huarong Huang
Summary: A simple and efficient method for inducing mouse embryonic stem cells into primitive endoderm cells was reported in this study, and it was found that the Wnt signaling pathway played a critical role in this process.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Erika Cosset, Youssef Hibaoui, Sten Ilmjarv, Pierre-Yves Dietrich, Caroline Tapparel, Karl-Heinz Krause
Summary: This study presents a standardized protocol for differentiating human embryonic stem cells into engineered neural tissue enriched with motor neurons, and highlights the role of motor neurons in poliovirus infection within these tissues. The research also reveals the molecular events occurring during PV-3 infection in the motor neurons and emphasizes the modulation of a set of genes involved in the EGR-EP300 complex.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Neurosciences
Carla Belmonte-Mateos, Cristina Pujades
Summary: The central nervous system exhibits a wide diversity of neurons with specific cell types and proportions in the appropriate locations. The coordination of proliferation and differentiation is crucial for generating brains with specific size and cell composition. Understanding how cell diversity arises from pluripotent progenitor cells and how progenitor potential changes over time is an important focus in developmental neurobiology.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Medicine, Research & Experimental
Mohaddeseh Sadat Alavi, Sajad Sahab Negah, Ahmad Ghorbani, Azar Hosseini, Hamid R. Sadeghnia
Summary: The study demonstrates that LEV can enhance the proliferation of rat embryonic neural stem cells and promote neuronal differentiation mainly through an NMDA receptor-mediated mechanism.
Article
Cell & Tissue Engineering
Yikai Huang, Ting Su, Congcong Wang, Lixia Dong, Shuang Liu, Yaru Zhu, Kunying Hao, Yin Xia, Qing Jiang, Jinzhong Qin
Summary: The deficiency of retinoblastoma binding protein 4 (RBBP4) in embryonic stem cells (ESCs) leads to spontaneous differentiation into mesendodermal lineages, and shares common genomic targets with the core Polycomb complex. RBBP4 is crucial for targeting PRC2 to a subset of developmental genes, and its presence is closely related to the maintenance of the pluripotency regulatory network and repression of mesendoderm lineages.
Article
Engineering, Biomedical
Lakshmi Kavitha Sthanam, Tanusri Roy, Sejal Patwardhan, Avi Shukla, Shipra Sharma, Pradip Shinde, Hanuman Tulasiram Kale, P. Chandra Shekar, Kiran Kondabagil, Shamik Sen
Summary: Stem cell differentiation is influenced by the interaction between cells and their extracellular matrix, with matrix metalloproteinases (MMPs) playing a role in this process. Differentiation of mouse embryonic stem cells (mESCs) on varying matrices led to changes in MMP expression and activity, affecting integrin expression, mechanotransduction pathways, and germ layer-specific markers. Inhibition of MMP activity altered the germ layer commitment of differentiating mESCs, demonstrating the importance of MMPs in regulating stem cell differentiation.
Article
Biochemistry & Molecular Biology
Shotaro Kawano, Masayuki Baba, Hotaka Fukushima, Daiki Miura, Hitoshi Hashimoto, Takanobu Nakazawa
Summary: This study analyzed the role of ANK2 in the developing mouse cerebral cortex and found that ANK2 regulates neural stem cell differentiation and neuronal migration. ANK2 haploinsufficiency may impair neural development and increase the risk of ASD in patients.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Chie-Pein Chen, Pei-Chun Chen, Yu-Ling Pan, Yi-Chao Hsu
Summary: The study shows that prenatal infection may contribute to the development of neuropsychiatric disorders in children. Exposure to lipopolysaccharide during prenatal development could lead to anxiety-like behavior and sensorineural hearing loss in offspring, as well as disruptions in neural differentiation. The abnormal differentiation of astrocytes and oligodendrocytes in the brain, potentially mediated by ApoB/E signaling pathways in response to inflammatory stimuli, may be responsible for the anxiety-like behavior observed in male offspring.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)
Article
Chemistry, Medicinal
Sietske Kooijman, Hilde Vrieling, Lisa Verhagen, Justin de Ridder, Alex de Haan, Elly van Riet, Albert J. R. Heck, Gideon F. A. Kersten, Jeroen L. A. Pennings, Bernard Metz, Hugo D. Meiring
Summary: This study compares the immune response induced by aluminum hydroxide and aluminum phosphate adjuvants in vitro and in vivo, revealing differences in their molecular mechanisms and regulation of innate immune processes.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Substance Abuse
Jeroen L. A. Pennings, Anne Havermans, Charlotte G. G. M. Pauwels, Erna J. Z. Krusemann, Wouter F. Visser, Reinskje Talhout
Summary: The presence of nicotine salt-containing e-liquids in the Netherlands market has increased significantly in recent years. Nicotine salts, due to their characteristics, make these e-liquids more addictive and attractive, particularly to young and inexperienced users. Policymakers are advised to regulate products containing nicotine salts.
Article
Substance Abuse
Jeroen L. A. Pennings, Geoffrey Ferris Wayne, Walther N. M. Klerx, Charlotte G. G. M. Pauwels, Reinskje Talhout
Summary: This study explores the impact of tobacco blend and design characteristics on the sensory experience of cigarette consumers. The findings suggest that while some variations in design are noticeable to consumers, many products on the market are not discernibly different, indicating that brand diversity may serve non-sensory purposes such as marketing.
Article
Food Science & Technology
Jeroen L. A. Pennings, Eric Abachin, Raphael Esson, Hennie Hodemaekers, Antoine Francotte, Jean-Baptiste Claude, Celine Vanhee, Sylvie Uhlrich, Rob J. Vandebriel
Summary: The study used RNA sequencing to identify RNA signatures related to consistent TeNT production by Clostridium tetani under standard and non-standard culture conditions. Time-dependent differentially expressed genes (DEGs) were identified, with the tetX gene showing potential as a transcriptional biomarker for monitoring TeNT production. Non-standard culture conditions led to different gene expression responses, highlighting the importance of batch-to-batch consistency testing in tetanus vaccine manufacturing.
Article
Biochemistry & Molecular Biology
Rob J. Vandebriel, Sylvie Remy, Jolanda P. Vermeulen, Evelien G. E. Hurkmans, Kirsten Kevenaar, Neus G. Bastus, Beatriz Pelaz, Mahmoud G. Soliman, Victor F. Puntes, Wolfgang J. Parak, Jeroen L. A. Pennings, Inge Nelissen
Summary: The study compared the effects of gold nanoparticles of different shapes on NLRP3 inflammasome activation and found that nanorods can activate the NLRP3 inflammasome, while nanostars and nanospheres cannot. Additionally, gold nanorod-induced NLRP3 inflammasome activation was accompanied by downregulated gene sets related to sterol/cholesterol biosynthesis, oxidative phosphorylation, and purinergic receptor signaling, with notable downregulation of Paraoxonase-2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Substance Abuse
Ingrid M. E. Bakker-'t Hart, Frank Bakker, Jeroen L. A. Pennings, Naomi Weibolt, Selma Eising, Reinskje Talhout
Summary: This study examines the commonly used flavors and flavorings in waterpipe tobacco and related products, and presents a waterpipe tobacco flavor wheel for experts' reference. The analysis reveals that the majority of waterpipe tobacco products are flavored, with fruit flavors being the most prevalent. Herbal molasses and steam stones contain similar levels of flavorings, with lower levels compared to waterpipe tobacco. The flavorings in waterpipe products are mainly fruity and sweet, often combined with menthol/mint flavorings. Banning flavorings in all waterpipe products could be an effective strategy to reduce waterpipe smoking among youth.
Correction
Immunology
Coen A. L. Stalpers, Irene A. Retmana, Jeroen L. A. Pennings, Rob J. Vandebriel, Coenraad F. M. Hendriksen, Arnoud M. Akkermans, Marcel H. N. Hoefnagel
Article
Pharmacology & Pharmacy
Marja Driessen, Suzanne van der Plas-Duivesteijn, Anne S. Kienhuis, Evert-Jan van den Brandhof, Marianne Roodbergen, Bob van de Water, Herman P. Spaink, Magnus Palmblad, Leo T. M. van der Ven, Jeroen L. A. Pennings
Summary: The zebrafish embryo is a promising model for studying hepatotoxicity and could potentially reduce the use of rodents in such assessments. Proteomics analysis identified a set of potential protein markers for detecting adverse liver responses.
Article
Reproductive Biology
Laura M. M. Samrani, Jeroen L. A. Pennings, Nina Hallmark, Remi Bars, Helen Tinwell, Marc Pallardy, Aldert H. Piersma
Summary: The study investigates the effect of exposure duration on gene expression changes in the all-trans retinoic acid (ATRA) signaling pathway in zebrafish embryotoxicity test (ZET). It is found that the highest magnitudes of gene expression regulation occur after 2-24 hrs exposure, with the optimal response at 4 hrs. Early assessment of gene expression regulation after exposure onset in ZET could be optimal for predicting developmental toxicity.
REPRODUCTIVE TOXICOLOGY
(2023)
Article
Substance Abuse
Jeroen L. A. Pennings, Anne Havermans, Erna J. Z. Krusemann, Dion Zijtveld, Eva H. W. Huiberts, Peter M. J. Bos, Erna Schenk, Wouter F. Visser, Ingrid M. EBakker-'t Hart, Yvonne C. M. Staal, Reinskje Talhout
Summary: The Dutch government plans to only allow tobacco flavors in e-liquids to discourage e-cigarette use among youth. Researchers have compiled a restrictive list of 16 tobacco flavorings to implement this policy.
Article
Psychiatry
M. Liset Rietman, N. Charlotte Onland-Moret, Astrid C. J. Nooyens, Dorina Ibi, Ko Willems van Dijk, Leonard Daniel Samson, Jeroen L. A. Pennings, Maarten Schipper, Albert Wong, Annemieke M. W. Spijkerman, Martijn E. T. Dolle, W. M. Monique Verschuren
Summary: Cognitive decline is a normal part of aging, but factors such as genetics and environment can cause some individuals to experience a faster decline. This study suggests that the decline in overall cognitive function is influenced by different mechanisms than those involved in regulating cognitive function.
TRANSLATIONAL PSYCHIATRY
(2022)
Review
Chemistry, Medicinal
Christina H. J. Veltman, Jeroen L. A. Pennings, Bob van de Water, Mirjam Luijten
Summary: Nongenotoxic (NGTX) carcinogens induce cancer through mechanisms other than direct DNA damage, primarily involving the induction of oxidative stress leading to regenerative proliferation. However, current carcinogenicity assessment methods mainly focus on genetic toxicity, which may overlook NGTX carcinogens. To improve the predictivity of carcinogenicity assessment, a mechanism-based approach such as the adverse outcome pathway (AOP) network for chemically induced oxidative stress leading to (NGTX) carcinogenesis is proposed. This AOP network considers the role of oxidative stress in cancer development and explores the mechanisms and biological effects of chemical-induced oxidative damage. Developing AOP networks relevant to human carcinogenesis will facilitate a transition to a mechanism-based, human-relevant carcinogenicity assessment with reduced reliance on laboratory animals.
CHEMICAL RESEARCH IN TOXICOLOGY
(2023)
Article
Neurosciences
Faycal Guedj, Elise Kane, Lauren A. Bishop, Jeroen L. A. Pennings, Yann Herault, Diana W. Bianchi
Summary: The effectiveness of the Ts65Dn mouse model in improving neurocognition in Down syndrome has not been successfully translated to humans. The Ts66Yah mouse model, which carries an extra chromosome and identical segmental trisomy as Ts65Dn, was used to investigate the underlying factors.
BIOLOGICAL PSYCHIATRY
(2023)
Article
Substance Abuse
Charlotte G. G. M. Pauwels, Wouter F. Visser, Jeroen L. A. Pennings, Ernesto P. Baloe, Arnout P. T. Hartendorp, Loes van Tiel, Mirjam van Mourik, Wim Vaessen, Sanne Boesveldt, Reinskje Talhout
Summary: This study aimed to determine whether nicotine salts increase sensory appeal at lower concentrations and compared the inhalation intensity of both types of e-liquids. The results showed that nicotine salts did not significantly affect sensory appeal, harshness, and puffing intensity compared to free-based nicotine.
DRUG AND ALCOHOL DEPENDENCE
(2023)
Article
Substance Abuse
Jeroen L. A. Pennings, Charlotte G. G. M. Pauwels, Erna Schenk, Reinskje Talhout
Summary: Cigarillos have emerged as a substitute for flavored cigarettes, which are prohibited in the European Union, and they are also cheaper. To gain a better understanding of the cigarillo market, we analyzed product data from manufacturers via the EU-CEG system.
TOBACCO INDUCED DISEASES
(2023)
