Article
Cell Biology
Anastasia M. M. Hughes, Vincent Kuek, Joyce Oommen, Grace-Alyssa Chua, Maria van Loenhout, Sebastien Malinge, Rishi S. S. Kotecha, Laurence C. C. Cheung
Summary: Components of the bone marrow microenvironment (BMM) play a significant role in the development, progression, and treatment response of acute lymphoblastic leukemia (B-ALL). This study investigated the cellular and transcriptome profiles of mesenchymal stem cells (MSCs) isolated from the BMM of an immunocompetent BCR-ABL1(+) model of B-ALL. The findings showed that leukemia-associated MSCs had reduced self-renewal capacity, upregulation of inflammatory signaling pathways, and downregulation of genes involved in extracellular matrix organization and osteoblastogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Immunology
Betsabel Chicana, Nastaran Abbasizadeh, Christian Burns, Hanna Taglinao, Joel A. Spencer, Jennifer O. Manilay
Summary: This study investigates the mechanisms of B cell defects in bone marrow caused by the loss of von-Hippel Lindau protein (VHL). The result shows that there is an increase in the frequency and number of B cell progenitors in VhlcKO bone marrow, while Fransctions B-F are decreased. VhlcKO B cells exhibit increased apoptosis and quiescence. Reciprocal BM chimeras confirm that the B cell defects in VhlcKO are derived from extrinsic sources.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Xirui Fan, Jinyi Zhou, Xintong Yan, Xiaowen Bi, Juanjuan Liang, Shuai Lu, Lan Luo, Da Zhou, Zhimin Yin
Summary: Citrate can suppress the growth of prostate cancer cells by inducing autophagic cell death, without remarkable toxicity to mice.
Article
Oncology
Gilberto Gastelum, Jeffry Kraut, Mysore Veena, Alisher Baibussinov, Christopher Lamb, Kylee Lyons, Eric Y. Chang, Patrick Frost
Summary: Hypoxia-induced apoptosis of MM cells is correlated with acidic intracellular pH. Targeting intracellular pH may be more effective at treating cancers within a hypoxic TME.
FRONTIERS IN ONCOLOGY
(2023)
Article
Cell Biology
Xiaoyong Chen, Jing Huang, Jun Wu, Jie Hao, Boqiang Fu, Ying Wang, Bo Zhou, Tao Na, Jun Wei, Yong Zhang, Qiyuan Li, Shijun Hu, Jiaxi Zhou, Junying Yu, Zhaohui Wu, Huanxin Zhu, Jiani Cao, Lei Wang, Yaojin Peng, Lingmin Liang, Aijin Ma, Yu Zhang, Tongbiao Zhao, Andy Peng Xiang
Summary: Mesenchymal stem cells (MSCs) have potential for cell therapy and tissue regeneration due to their properties, but their heterogeneity poses challenges. The "Human Mesenchymal Stem Cells" guidelines provide standards for quality control and technical specifications, aiming to accelerate the international standardization of MSCs for clinical development and therapeutic applications.
CELL PROLIFERATION
(2022)
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Article
Endocrinology & Metabolism
Hao Zhang, Renkai Wang, Guangchao Wang, Bo Zhang, Chao Wang, Di Li, Chen Ding, Qiang Wei, Zhenyu Fan, Hao Tang, Fang Ji
Summary: B cells in the bone marrow microenvironment play a crucial role in fracture healing by inhibiting excessive bone regeneration through the production of osteoblast inhibitors. Their numbers fluctuate during different stages of fracture healing, impacting the overall bone regrowth process.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Immunology
Encarnacion Montecino-Rodriguez, Kenneth Dorshkind
Summary: Traditional models of lymphopoiesis present B and T cell development as a linear process, but studies have shown that the types of lymphocytes generated before and after birth differ. This review synthesizes data from multiple laboratories, including our own work using mouse models, and demonstrates that innate and conventional B and T cells emerge in different ways and are regulated differently. Understanding this layering of lymphocyte development has implications for the composition of the adult immune system and the origin of lymphocytic leukemias.
IMMUNOLOGICAL REVIEWS
(2023)
Article
Immunology
Chiranjeevi Tikka, Lindsay Beasley, Chengxian Xu, Jing Yang, Scott Cooper, Joseph Lechner, Sarah Gutch, Mark H. Kaplan, Maegan Capitano, Kai Yang
Summary: The transcription factor BATF is essential for maintaining homeostasis and functionality of bone marrow regulatory T cells (BM Tregs), which are crucial for hematopoiesis and B cell development. Lack of BATF leads to reduced expression of Treg effector molecules and compromised proportions of BM Tregs, while increasing the numbers of hematopoietic stem cells, multipotent progenitors, and granulocyte-macrophage progenitors. BATF deficiency also impairs the functionality of myeloid progenitors and the generation of common lymphoid progenitors, and fails to support B cell development in the bone marrow. Mechanistically, BATF mediates IL-7 signaling to promote expression of effector molecules on BM Tregs and maintain their homeostasis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ah-Won Kwak, Woo-Keun Kim, Seung-On Lee, Goo Yoon, Seung-Sik Cho, Ki-Taek Kim, Mee-Hyun Lee, Yung Hyun Choi, Jin-Young Lee, Jin Woo Park, Jung-Hyun Shim
Summary: This study aims to investigate the antitumor potential and molecular mechanism of Licochalcone B (LCB) in human colorectal cancer cells. The results demonstrate that LCB has a potential therapeutic effect against colorectal cancer cells through the ROS-mediated JNK/p38 MAPK signaling pathway, making it a promising anticancer therapeutic and prophylactic agent.
Article
Marine & Freshwater Biology
Xia Zhao, Xu Shi, Qingqing Liu, Xiaojing Li
Summary: The present study demonstrates the effects of acetochlor exposure on fish cells, including oxidative stress, mitochondrial damage, lactate accumulation, and cell death. However, tea polyphenols can alleviate these issues and protect cells from oxidative stress.
AQUATIC TOXICOLOGY
(2022)
Article
Cell Biology
Lan Huang, Xizhou An, Yao Zhu, Kainan Zhang, Li Xiao, Xinyuan Yao, Xing Zeng, Shaoyan Liang, Jie Yu
Summary: This study found that in B-cell acute lymphoblastic leukemia (B-ALL), netrin-1 induces an anti-apoptotic effect in B-ALL cells by activating the FAK-MAPK signaling pathway. These findings reveal the oncogenic role of netrin-1 in B-ALL.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Medicine, General & Internal
Bin Jia, Yaping Jiang, Yao Yao, Yingxing Xu, Yingzhen Wang, Tao Li
Summary: Perturbations in bone marrow mesenchymal stem cell (BMSC) differentiation are important in steroid-induced osteonecrosis of the femoral head (SONFH). Baicalin (BA), a compound in traditional Chinese medicine, can affect cell proliferation and apoptosis via the hedgehog (HH) signaling pathway. This study aimed to explore the role of BA in dexamethasone (Dex)-induced BMSC apoptosis and its mechanisms.
CHINESE MEDICAL JOURNAL
(2023)
Article
Immunology
Barbara Bassani, Claudio Tripodo, Paola Portararo, Alessandro Gulino, Laura Botti, Claudia Chiodoni, Elena Jachetti, Niccolo Bolli, Marilena Ciciarello, Korinna Joehrens, Ioannis Anagnostopoulos, Il-Kang Na, Antonio Curti, Mario P. Colombo, Sabina Sangaletti
Summary: CD40+ BM-MSCs within the bone marrow play a crucial role in immune regulation, with loss of CD40 leading to uncontrolled T cell activation due to reduced Treg numbers and impaired B-cell development. This study highlights the importance of CD40 in maintaining Treg homeostasis and proper B-cell development in the context of bone marrow transplantation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Zheng Li, Xiao Wang, Tian-pei Hong, Hao-jie Wang, Zhan-yi Gao, Meng Wan
Summary: In this study, it was found that advanced glycosylation end products (AGEs) significantly inhibited the proliferation of BMSCs through activating the MAPK pathway. The concentration and duration of AGEs were significantly correlated with cell viability of BMSCs, with the most appropriate concentration being 0.25 mg/ml. Stimulation with AGEs increased the protein expression levels of NF-kappa B p65, JNK, and P38, while decreasing I kappa B, indicating the involvement of the NF-kappa B and MAPK signaling pathways in the mechanism of action.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2021)
Review
Environmental Sciences
Scott Auerbach, Dayne Filer, David Reif, Vickie Walker, Alison C. Holloway, Jennifer Schlezinger, Supriya Srinivasan, Daniel Svoboda, Richard Judson, John R. Bucher, Kristina A. Thayer
ENVIRONMENTAL HEALTH PERSPECTIVES
(2016)
Article
Toxicology
Alicia M. Bolt, Michael P. Grant, Ting Hua Wu, Manuel Flores Molina, Dany Plourde, Alexander D. R. Kelly, Luis Fernando Negro Silva, Maryse Lemaire, Jennifer J. Schlezinger, Fackson Mwale, Koren K. Mann
TOXICOLOGICAL SCIENCES
(2016)
Article
Toxicology
James Watt, Thomas F. Webster, Jennifer J. Schlezinger
TOXICOLOGICAL SCIENCES
(2016)
Article
Toxicology
Amelia H. Baker, Ting Hua Wu, Alicia M. Bolt, Louis C. Gerstenfeld, Koren K. Mann, Jennifer J. Schlezinger
TOXICOLOGICAL SCIENCES
(2017)
Article
Toxicology
Stephanie Kim, Amy Li, Stefano Monti, Jennifer J. Schlezinger
ARCHIVES OF TOXICOLOGY
(2018)
Article
Cell Biology
James Watt, Amelia H. Baker, Brett Meeks, Paola D. Pajevic, Elise F. Morgan, Louis C. Gerstenfeld, Jennifer J. Schlezinger
JOURNAL OF CELLULAR PHYSIOLOGY
(2018)
Article
Cell Biology
Beth Bragdon, Robert Burns, Amelia H. Baker, Anna C. Belkina, Elise F. Morgan, Gerald V. Denis, Louis C. Gerstenfeld, Jennifer J. Schlezinger
JOURNAL OF CELLULAR PHYSIOLOGY
(2015)
Article
Multidisciplinary Sciences
Christopher D. Kassotis, Lauren Masse, Stephanie Kim, Jennifer J. Schlezinger, Thomas F. Webster, Heather M. Stapleton
SCIENTIFIC REPORTS
(2017)
Article
Biology
Thomas F. Webster, Jennifer J. Schlezinger
MATHEMATICAL BIOSCIENCES
(2019)
Article
Toxicology
Stephanie Kim, Nabil Rabhi, Benjamin C. Blum, Ryan Hekman, Kieran Wynne, Andrew Emili, Stephen Farmer, Jennifer J. Schlezinger
ARCHIVES OF TOXICOLOGY
(2020)
Article
Toxicology
Jennifer J. Schlezinger, Wendy Heiger-Bernays, Thomas F. Webster
TOXICOLOGICAL SCIENCES
(2020)
Article
Pharmacology & Pharmacy
Christopher D. Kassotis, Kate Hoffman, Johannes Volker, Yong Pu, Almudena Veiga-Lopez, Stephanie M. Kim, Jennifer J. Schlezinger, Patrizia Bovolin, Erika Cottone, Astrid Saraceni, Rosaria Scandiffio, Ella Atlas, Karen Leingartner, Stacey Krager, Shelley A. Tischkau, Sibylle Ermler, Juliette Legler, Vesna A. Chappell, Suzanne E. Fenton, Fahmi Mesmar, Maria Bondesson, Mariana F. Fernandez, Heather M. Stapleton
Summary: This study assessed the interlaboratory variability of efficacy and potency outcomes for MDCs using the 3T3-L1 preadipocyte cell assay. Results showed considerable differences in bioactivities reported across laboratories and test conditions, with greater consistency observed for the presence or absence of activity for each tested chemical. Developing a standardized adipogenic differentiation protocol was identified as the best strategy for improving consistency of adipogenic responses and reproducibly identifying MDCs.
Article
Gastroenterology & Hepatology
Partho Sen, Sami Qadri, Panu K. Luukkonen, Oddny Ragnarsdottir, Aidan McGlinchey, Sirkku Jantti, Anne Juuti, Johanna Arola, Jennifer J. Schlezinger, Thomas F. Webster, Matej Oresic, Hannele Yki-Jarvinen, Tuulia Hyotylainen
Summary: This study reveals that exposure to specific environmental contaminants like PFAS affects metabolic processes associated with NAFLD, and females may be more susceptible to the harmful impacts of PFAS.
JOURNAL OF HEPATOLOGY
(2022)
Article
Pharmacology & Pharmacy
Greylin Nielsen, Wendy J. Heiger-Bernays, Jennifer J. Schlezinger, Thomas F. Webster
Summary: The study highlights the potential impact of PFAS on human health, particularly the lack of data on the effects of PFAS mixtures on relevant molecular initiating events in the body. Experimental findings suggest that the GCA method is the most accurate in predicting the effects of PFAS mixtures on hPPARa activity, aiding in a more precise assessment of the potential risks posed by PFAS mixtures.
Letter
Medicine, Legal
Gloria B. Post, Linda S. Birnbaum, Jamie C. DeWitt, Helen Goeden, Wendy J. Heiger-Bernays, Jennifer J. Schlezinger
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2022)