Article
Biochemistry & Molecular Biology
Donato Cosco, Federica Bruno, Germano Castelli, Roberto Puleio, Sonia Bonacci, Antonio Procopio, Domenico Britti, Massimo Fresta, Fabrizio Vitale, Donatella Paolino
Summary: After infecting mice with Leishmania infantum, PLA nanocapsules loaded with meglumine antimoniate demonstrate significant antileishmanial activity, reducing the need for administration and decreasing parasite load in liver, spleen, and kidneys, thus showing potential as a novel nanomedicine for leishmaniasis treatment.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Infectious Diseases
Luciana Mendes, Jorge Oliveira Guerra, Bleno Costa, Arineia Soares da Silva, Maria das Gracas Barbosa Guerra, Jessica Ortiz, Susan Smith Doria, George Villarouco da Silva, Denison Vital de Jesus, Manoel Barral-Netto, Gerson Penna, Edgar M. Carvalho, Paulo R. L. Machado
Summary: This study compared different treatment regimens in 150 patients with cutaneous leishmaniasis caused by Leishmania guyanensis and found that miltefosine did not have a higher cure rate than pentavalent antimony. Despite the lack of improvement in therapeutic response with the addition of GM-CSF, miltefosine should remain a main drug for treating CL due to its lower toxicity, easier administration, and similar cure rate compared to pentavalent antimony.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Parasitology
Lucas Sousa Magalhaes, Lays Gisele Santos Bomfim, Camilla Natalia Oliveira Santos, Priscila Lima dos Santos, Diego Moura Tanajura, Michael Wheeler Lipscomb, Amelia Ribeiro de Jesus, Roque Pacheco de Almeida, Tatiana Rodrigues de Moura
Summary: The study found that antimony-resistant L. infantum isolates can persist during the early stages of infection, and the activation of macrophages or blocking of thiol production can enhance parasite clearance, which is associated with an increase in the production of pro-inflammatory cytokines.
PARASITOLOGY RESEARCH
(2021)
Review
Public, Environmental & Occupational Health
Matteo Piccica, Filippo Lagi, Alessandro Bartoloni, Lorenzo Zammarchi
Summary: A systematic review on the efficacy and safety of pentamidine isethionate for human tegumentary and visceral leishmaniasis treatment found similar cure rates to first-line drugs with fewer severe and irreversible adverse effects, making it a potential alternative for patients who fail to respond to or tolerate other medications.
JOURNAL OF TRAVEL MEDICINE
(2021)
Article
Parasitology
Reza Somee, Gilda Eslami, Mahmood Vakili
Summary: This study evaluated the gene expressions of MAPK and AQP1 in treatment failure clinical isolates of Leishmania major, showing significantly lower expressions in AQP1 and MAPK1 in treatment failure isolates compared to treatment response isolates. Additionally, a nucleotide change in the AQP1 gene sequence was observed in treatment failure isolates.
ACTA PARASITOLOGICA
(2022)
Article
Infectious Diseases
Masoumeh Tayyebi, Emadodin Darchini-Maragheh, Pouran Layegh, Bita Kiafar, Vahid Mashayekhi Goyonlo
Summary: Leishmaniasis is a common disease in Iran, with cutaneous leishmaniasis being a major health concern. Some patients show resistance to traditional treatment methods like meglumine antimoniate. Oral miltefosine may serve as an effective alternative treatment.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Pharmacology & Pharmacy
Vanessa Ortega, Allan Radaic, Marcelo Bispo de Jesus, Eneida de Paula, Selma Giorgio
Summary: Liposomal AME is a promising delivery system for treating visceral leishmaniasis, improving meglumine efficacy against L. infantum and minimizing its cytotoxicity towards canine macrophages.
JOURNAL OF PHARMACY AND PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Anabel Zabala-Penafiel, Geovane Dias-Lopes, Lea Cysne-Finkelstein, Fatima Conceicao-Silva, Luciana de Freitas Campos Miranda, Aline Fagundes, Armando de Oliveira Schubach, Maria Ines Fernandes Pimentel, Franklin Souza-Silva, Lucas de Almeida Machado, Carlos Roberto Alves
Summary: Glucantime (Sb-V) is the first-line treatment for American Tegumentary Leishmaniasis, but resistance cases have been reported. This study analyzed 12 Leishmania (Viannia) braziliensis isolates from patients with different treatment outcomes, showing phenotypic heterogeneity and potential for characterization based on in vitro susceptibility to Sb-III and serine proteases profiles.
SCIENTIFIC REPORTS
(2021)
Review
Biotechnology & Applied Microbiology
Meliana Borilli Pereira, Bruna Gomes Sydor, Karla Gabriela Memare, Thais Gomes Verzignassi Silveira, Sandra Mara Alessi Aristides, Eduardo Monguilhott Dalmarco, Jorge Juarez Vieira Teixeira, Maria Valdrinez Campana Lonardoni, Izabel Galhardo Demarchi
Summary: The study explored the evidence of encapsulated meglumine antimoniate for cutaneous leishmaniasis treatment using nanotechnology in animal models. Various nanoparticles showed significant efficacy in BALB/c mice against different strains of Leishmania, but the limited number of studies and potential bias suggest the need for high-quality preclinical research in the future.
Article
Parasitology
Mahmoud Nateghi-Rostami, Minoo Tasbihi, Fatemeh Darzi
Summary: Clinical resistance to pentavalent antimonial compounds is a major problem in the treatment of human leishmaniasis. The metabolism and activity of thiol, as well as the activity of enzymes, play a central role in this resistance.
Review
Infectious Diseases
Janaina de Pina Carvalho, Sarah Nascimento Silva, Mariana Lourenco Freire, Lindicy Leidicy Alves, Carolina Senra Alves de Souza, Glaucia Cota
Summary: This review provides an overview of the clinical experience in the Americas related to the treatment of ML and identifies interventions and combinations that are eligible for exploration in future well-designed studies.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Multidisciplinary Sciences
Tiget Ayelgn Mengstie, Hiwot Tezera Endale, Tadele Mulaw, Aman Mossa Abdella, Rezika Mohammed, Tabarak Malik, Gashaw Dessie
Summary: This study found that VL patients treated with sodium stibogluconate and paromomycin combined drugs had elevated serum amylase and lipase levels during treatment, with significant associations between age and serum amylase and lipase.
Article
Multidisciplinary Sciences
Tiget Ayelgn Mengstie, Hiwot Tezera Endale, Tadele Mulaw, Aman Mossa Abdella, Rezika Mohammed, Tabarak Malik, Gashaw Dessie
Summary: The study found a significant increase in serum amylase and lipase levels after treatment with sodium stibogluconate and paromomycin combined drugs in VL patients, indicating potential drug-induced adverse effects and associated risk factors.
Article
Microbiology
Maria del Mar Castro, Alexandra Cossio, Adriana Navas, Olga Fernandez, Liliana Valderrama, Lyda Cuervo-Pardo, Ricardo Marquez-Onate, Maria Adelaida Gomez, Nancy Gore Saravia
Summary: The addition of pentoxifylline to standard treatment did not show clinical benefits in patients with early mild to moderate cutaneous leishmaniasis. However, therapeutic failure was associated with overexpression of certain inflammatory genes.
Article
Infectious Diseases
Ana Isabel Olias-Molero, Pedro Botias, Montserrat Cuquerella, Jesus Garcia-Cantalejo, Emilia Barcia, Susana Torrado, Juan Jose Torrado, Jose Maria Alunda
Summary: This study investigated the impact of clindamycin on the intestinal microbiome and the availability and distribution of miltefosine in target organs. It was found that clindamycin significantly altered the relative abundance of the intestinal microbiome, but had no clear effect on the availability of miltefosine.
Article
Veterinary Sciences
Alessandro Ferrari, Marzia Cozzi, Luca Aresu, Valeria Martini
Summary: This case study of an 8-year-old spayed female Beagle dog with large B-cell lymphoma (LBCL) highlights the importance of accurate diagnosis through lymph node cytology and flow cytometry. The presence of neoplastic cells in both peripheral blood and bone marrow demonstrates the complexity of the disease, requiring a comprehensive treatment approach.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2021)
Article
Veterinary Sciences
Luca Aresu, Chiara Agnoli, Arturo Nicoletti, Antonella Fanelli, Valeria Martini, Francesco Bertoni, Laura Marconato
Summary: Canine Burkitt-like lymphoma (B-LL) is a rare tumor in dogs classified as a high-grade B-cell malignancy. Compared to the human counterpart, phenotypical differences are observed, and despite treatment, prognosis remains poor. However, around 30% of dogs can survive more than 1 year.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Massimo Salvi, Filippo Molinari, Selina Iussich, Luisa Vera Muscatello, Luca Pazzini, Silvia Benali, Barbara Banco, Francesca Abramo, Raffaella De Maria, Luca Aresu
Summary: ARCTA is a fully automated algorithm developed for the classification and grading of canine tumors, exhibiting excellent performance in both RCT classification and mast cell tumors grading in the test set.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Valeria Martini, Giuseppe Marano, Luca Aresu, Ugo Bonfanti, Patrizia Boracchi, Mario Caniatti, Francesco Cian, Matteo Gambini, Laura Marconato, Carlo Masserdotti, Arturo Nicoletti, Fulvio Riondato, Paola Roccabianca, Damiano Stefanello, Erik Teske, Stefano Comazzi
Summary: Cytopathology is a valuable tool for diagnosing lymphoma in dogs, with high accuracy for overall lymphoma diagnosis but lower accuracy for subtype characterization. It can be used as a screening test to predict grade and phenotype, but confirmation with additional techniques like flow cytometry and immunohistochemistry is necessary. Interobserver agreement for identifying lymphoma cases and subtype characterization was moderate.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2022)
Article
Oncology
Katia Pinello, Valeria Baldassarre, Katja Steiger, Orlando Paciello, Isabel Pires, Renee Laufer-Amorim, Anna Oevermann, Joao Niza-Ribeiro, Luca Aresu, Brian Rous, Ariana Znaor, Ian A. Cree, Franco Guscetti, Chiara Palmieri, Maria Lucia Zaidan Dagli
Summary: Researchers have developed a comparative coding system for canine neoplasms that is compatible with the human coding system, enabling the collection of consistent epidemiological canine cancer data and facilitating comparative oncology studies.
Article
Veterinary Sciences
Cecilia Gola, Luca Licenziato, Paolo Accornero, Selina Iussich, Emanuela Morello, Paolo Buracco, Paola Modesto, Luca Aresu, Raffaella De Maria
Summary: This study aimed to evaluate the expression of PLK1 and c-Myc in canine osteosarcoma and study the effects of PLK1 inhibitor BI 2536 on a c-Myc overexpressing cell line. The results showed that c-Myc overexpression was associated with reduced overall survival and BI 2536 could inhibit cell growth and decrease c-Myc protein levels. These findings suggest the prognostic value of c-Myc in canine osteosarcoma and the potential of PLK1 as a therapeutic target for tumors overexpressing c-Myc.
VETERINARY AND COMPARATIVE ONCOLOGY
(2022)
Review
Agriculture, Dairy & Animal Science
Valentina Moccia, Alessandro Sammarco, Laura Cavicchioli, Massimo Castagnaro, Laura Bongiovanni, Valentina Zappulli
Summary: This article summarizes the research literature on extracellular vesicles (EVs) in animals between 1984 and 2021, focusing on approximately 220 scientific papers. The study finds that EVs play important roles in animal physiology and pathophysiology, disease diagnosis markers, and potential transporters for therapies and vaccines. The article also highlights the significance of EVs shed within animal milk.
Article
Oncology
Lucia Minoli, Luca Licenziato, Mikolaj Kocikowski, Marzia Cino, Katarzyna Dziubek, Selina Iussich, Antonella Fanelli, Emanuela Morello, Marina Martano, Ted Hupp, Borek Vojtesek, Maciej Parys, Luca Aresu
Summary: In this study, specific monoclonal antibodies targeting canine PD-1 and PD-L1 were developed and validated in canine anal sac adenocarcinoma. The results suggest that the canine PD-1/PD-L1 axis is relevant in this tumor and inhibition might be an effective strategy after surgery.
Article
Agriculture, Dairy & Animal Science
Elisa Padoan, Serena Ferraresso, Sara Pegolo, Carlo Barnini, Massimo Castagnaro, Luca Bargelloni
Summary: This study comprehensively defined the transcriptomic fingerprints of mild and severe equine asthma using gene expression analysis. The pathogenesis of equine asthma involves reduced mucociliary clearance and excessive complement activation leading to tissue injury. Furthermore, severe equine asthma is characterized by chronic inflammation and tissue remodeling similar to human asthma.
Article
Veterinary Sciences
Laura Marconato, Damiano Stefanello, Fabrizio Solari Basano, Eugenio Faroni, Mauro Dacasto, Mery Giantin, Giuliano Bettini, Luca Aresu, Ugo Bonfanti, Walter Bertazzolo, Maurizio Annoni, Cristina Lecchi, Silvia Sabattini
Summary: This study aims to find prognostic markers for canine subcutaneous mast cell tumors (ScMCTs). The results show that high cytograde, a mitotic count greater than 4/10 high-power fields (hpf), and a Ki67-index greater than 23 are closely associated with the aggressiveness of ScMCTs. However, regional lymphadenectomy was performed on only a subset of dogs, and further research is needed to validate these findings.
Article
Veterinary Sciences
Alessia Candellone, Paola Badino, Flavia Girolami, Matteo Cerquetella, Patrizia Nebbia, Luca Aresu, Simona Zoppi, Domenico Bergero, Rosangela Odore
Summary: The use of unbalanced, poultry-based raw diets for dogs has increased in recent years, leading to potential risks of bacterial infections for both pets and humans. This article presents a case of Campylobacter infection in a puppy and its owner, highlighting the importance of a collaborative approach among veterinarians, physicians, and caregivers to prevent the spread of zoonotic diseases.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Maria Ines Crescio, Giuseppe Ru, Luca Aresu, Elena Bozzetta, Maria Giovanna Cancedda, Katia Capello, Massimo Castagnaro, Azzurra Carnio, Cristiano Cocumelli, Barbara Degli Uberti, Claudia Eleni, Greta Foiani, Niccolo Fonti, Lucia Rita Gibelli, Lorella Maniscalco, Elisabetta Manuali, Valentina Moccia, Orlando Paciello, Antonio Petrella, Antonio Petrini, Alessandro Poli, Roberto Puleio, Elisabetta Razzuoli, Paola Scaramozzino, Katia Varello, Marta Vascellari, Valentina Zappulli, Angelo Ferrari
Summary: This study described the data collected by The Italian Network of Laboratories for Veterinary Oncology in the first 9 years of activity, analyzing the frequency of tumour sites in dogs and considering risk factors. The results highlighted differences in tumour sites and potential role of risk factors. This emphasizes the importance of animal cancer registration initiatives to deepen knowledge of tumour epidemiology in dogs and humans.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
Elena Celant, Laura Marconato, Damiano Stefanello, Pierangelo Moretti, Luca Aresu, Stefano Comazzi, Valeria Martini
Summary: Phenotypic aberrancies in canine lymphomas were retrospectively studied, and their clinical and clinical pathological features at diagnosis were described. Different aberrant phenotypes were found in different lymphoma subtypes, providing clues for the diagnosis and prognosis of lymphoma.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
Francesco Albanese, Francesca Abramo, Michele Marino, Maria Massaro, Laura Marconato, Lucia Minoli, Valeria Martini, Luca Aresu
Summary: This study describes clinicopathological, immunohistochemical, and clonality features of cutaneous lymphocytosis (CL) in cats and dogs. Cats with CL showed prolonged survival regardless of treatment, while dogs had a shorter and more variable clinical course, suggesting a more challenging discrimination between CL and cutaneous epitheliotropic lymphoma in dogs.
VETERINARY SCIENCES
(2022)