Article
Nanoscience & Nanotechnology
Jyoti, Jose Munoz, Martin Pumera
Summary: Graphene quantum dot-based microrobots demonstrate potential for optical real-time monitoring of DNA, offering high sensitivity and selectivity.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Microbiology
Xinyue Wang, Bin Yang, Shuangshuang Ma, Xiaolin Yan, Shuai Ma, Hongmin Sun, Yuyang Sun, Lingyan Jiang
Summary: Lactate promotes Salmonella intracellular replication and systemic infection by driving macrophage M2 polarization.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Jeng-Chang Chen, Liang-Shiou Ou, Ming-Ling Kuo, Li-Yun Tseng, Hsueh-Ling Chang, Shiang-Chi Chen, Cheng-Hsun Chiu
Summary: Fetal exposure to microbial antigens or adoptive transfer of microbial antigen-loaded fetal macrophages can provide antigen-specific adaptive immunity, but protective immunity against lethal Salmonella challenge is only granted to those exposed to heat-killed Salmonella antigens.
Article
Chemistry, Analytical
Yawen He, Fei Jia, Yuxin Sun, Weihuan Fang, Yanbin Li, Juhong Chen, Yingchun Fu
Summary: In this study, an electrochemical sensing method was developed to detect S. Typhimurium using PCR and CRISPR / Cas12a technology. The method allows sensitive detection of the pathogen in pure cultures and spiked poultry meat.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Nanoscience & Nanotechnology
Qiwei Wang, Tai Fang, Jiao Zheng, Lin Shi, Lili Shi, Tao Li
Summary: Fluorescent molecular rotors were utilized to develop a novel high-performance FRET system, showing promise for DNA aptasensors with large Stokes shift, high photostability, and pH insensitivity. The efficient FRET effect between two dyes labeled on DNA demonstrated the potential of proximity-induced fluorescent ATP aptasensors with pH-switch functionality. The study also provided new insights into DNA proximity reactions and their roles in physiological processes through systematic investigation of the proximity effect by altering the linker length of the switching unit.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
M. S. Bacchu, M. R. Ali, S. Das, S. Akter, H. Sakamoto, S- Suye, M. M. Rahman, K. Campbell, M. Z. H. Khan
Summary: An electrochemical DNA biosensor was fabricated for the selective identification of S. Typhi in real samples. The biosensor showed a low limit of detection and the ability to be reused multiple times. The experimental results demonstrated the high feasibility and accuracy of using this biosensor for detecting S. Typhi in clinical and food samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Materials Science, Multidisciplinary
Xin Li, Yi Lu, Yong Hu
Summary: With the innovation of DNA nanotechnology, the rational design of DNA hydrogels has allowed for the development of DNA hydrogel biosensors with stimuli-responsiveness, tunable physicochemical properties, and integrated functionality. This has the potential to greatly impact biomedical applications.
Article
Chemistry, Applied
Jiafeng Pan, Zhi Liu, Junhua Chen
Summary: A biosensor for BPA detection was developed by integrating an amplifying DNA circuit with Mg2+-dependent DNAzyme in the sensing system, achieving a high sensitivity with a detection limit of 50 fM. The method proved to be selective and accurate, even working well in milk samples. The biosensor results were validated by LC-MS, showing no significant difference with the analytical method.
Article
Chemistry, Analytical
Han Du, Xu Zhang, Mingru Yao, Qingli Yang, Wei Wu
Summary: This study successfully constructed aptamer-guided luminous microspheres using carbon dots fluorescence, which can efficiently recognize and detect Salmonella enterica serovar Typhimurium. The method shows high sensitivity and has broad application prospects.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Immunology
Dan Xiong, Li Song, Yushan Chen, Xinan Jiao, Zhiming Pan
Summary: Salmonella Enteritidis is a significant intracellular pathogen that can cause gastroenteritis and pose a threat to life and health. This study evaluated the impact of Salmonella pathogenicity island-1 (SPI-1) and SPI-2 on S. Enteritidis virulence in vitro and in vivo, as well as the host inflammatory pathways affected by these SPIs. The results showed that both SPI-1 and SPI-2 contributed to bacterial invasion and proliferation in macrophages, induced cytotoxicity and cellular apoptosis, and triggered inflammatory responses mediated by ERK and STAT2 pathways. In a mouse infection model, SPI-2 played a crucial role in bacterial virulence by causing significant production of inflammatory cytokines and interferon-stimulated genes. Delta SPI-1 mutant mice showed moderate histopathological damage and reduced bacterial loads, while Delta SPI-2 and Delta SPI-1/SPI-2 mutant mice displayed minimal damage and no bacteria. A survival assay demonstrated that SPI-2 played a decisive role in bacterial virulence. Overall, both SPIs, especially SPI-2, profoundly contributed to S. Enteritidis intracellular localization and virulence by activating multiple inflammatory pathways.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Katarzyna M. Sitnik, Fran Krstanovic, Natascha Goedecke, Ulfert Rand, Tobias Kubsch, Henrike Maass, Yeonsu Kim, Ilija Brizic, Luka Cicin-Sain
Summary: To date, no herpesvirus has been found to persist in fibroblastic cells, but this study reveals that murine cytomegalovirus can persist in PDGFR alpha-positive fibroblastic cells for a long time and across organs. These cells not only support latent infection but also productive virus replication, and the presence of STAT1 is required for the virus to persist in vivo. This study highlights the dual role of fibroblastic cells as both a reservoir for latent virus and a site for replication.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Heyjin Son, Jaeil Park, Injoo Hwang, Youngri Jung, Sangsu Bae, Sanghwa Lee
Summary: In the study, two conformational intermediates were identified in the process of Cas12a cleaving double-stranded DNA target, including PAM-distal DNA unwound conformation and the binding of the target strand to the catalytic site. These findings highlighted the importance of Mg2+ ions in promoting the binding and cleavage of the target strand, and proposed a Mg2+-dependent kinetic model for the mechanism of Cas12a achieving cleavage of the target DNA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Y. H. Sun, G. Luxardi, G. Xu, K. Zhu, B. Reid, B. P. Guo, C. B. Lebrilla, E. Maverakis, M. Zhao
Summary: Salmonella infection alters the electrical properties of macrophages by modifying the host cell surface glycan composition, affecting the directional migration and phagocytosis of macrophages.
INFECTION AND IMMUNITY
(2022)
Review
Immunology
Olga Matveeva, Yury Nechipurenko, Denis Lagutkin, Yegor E. Yegorov, Julia Kzhyshkowska
Summary: Our review summarizes the evidence that COVID-19 can be complicated by SARS-CoV-2 infection of immune cells. Research has shown that some immune cells, including monocytes, macrophages, and B-cells, can be infected with the virus directly or through antibody-mediated or enhanced mechanisms. This infection can lead to a cascade of events, including host cell pyroptosis and inflammation. While more research is needed, this evidence highlights the importance of understanding the role of immune cell infection in COVID-19.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Yanping Wu, Aixin Hu, Xin Shu, Wenxia Huang, Ruiqiang Zhang, Yinglei Xu, Caimei Yang
Summary: This study elucidated the mechanisms of Lactobacillus plantarum (LP)-derived postbiotics in protecting animals against Salmonella infection by activating autophagy. LP postbiotics triggered autophagy to suppress the adhesion, invasion, replication, inflammation, and NLRP3 inflammasome activation of Salmonella. This study highlights the effectiveness of postbiotics in preventing Salmonella infection and provides a new strategy for intervention.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)