Article
Engineering, Biomedical
Yao Liu, Xiaonong Zhang, Chunsheng Xiao, Bin Liu
Summary: Peripheral nerve injury (PNI) is a complex disease that commonly affects young adults and has limited treatment options and poor clinical outcomes. Tissue-engineered hydrogels have shown promising results in promoting neural tissue regeneration and recovery of impaired neural function. This review discusses recent advances and various therapeutic strategies of hydrogels in peripheral nerve repair, as well as the prospects and challenges in their application for PNI treatment.
MATERIALS TODAY BIO
(2023)
Review
Biochemistry & Molecular Biology
Clelia Dispenza, Daniela Giacomazza, Mats Jonsson
Summary: Bio-hybrid hydrogels utilize radiation technologies to enhance biological activity, control chemical reactions, and have potential sterilization functions. They can serve as delivery devices for therapeutic biomolecules and create hydrogel patterns on surfaces.
Article
Materials Science, Multidisciplinary
Pramod Dorishetty, Rajkamal Balu, Amy Gelmi, Jitendra P. Mata, Anita Quigley, Naba K. Dutta, Namita Roy Choudhury
Summary: In this study, a regenerated silk fibroin/reduced graphene oxide hybrid ink system was developed and used to fabricate hydrogels with tunable properties and cell-gel interactions through extrusion printing and photocrosslinking. The incorporation of isopropyl alcohol (IPA) and increase in rGO content affected the viscosity, contact angle, printing accuracy, and physical properties of the hybrid ink and hydrogels. The increase in rGO content also improved the crosslink density, mechanical properties, and decreased the micropore size of the hybrid hydrogels. Furthermore, the hybrid and bioprinted hydrogels showed good biocompatibility with marginal difference in cell viability. These findings suggest the potential application of the developed hydrogel systems in tissue engineering and other functional applications.
MATERIALS TODAY ADVANCES
(2022)
Article
Medicine, Research & Experimental
Xiaolin Wang, Jing Li, Yongqian Bian, Congying Zhao, Jinqing Li, Xueyong Li
Summary: This study investigated the role of pH in regulating capillary lumen diameter. The results showed that changing the pH of the culture medium affected the cytosolic pH of endothelial cells, leading to changes in vascular diameter and capillary formation in tissue engineering.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Multidisciplinary Sciences
Ulrich Blache, Eden M. Ford, Byunghang Ha, Laura Rijns, Ovijit Chaudhuri, Patricia Y. W. Dankers, April M. Kloxin, Jess G. Snedeker, Eileen Gentleman
Summary: This article discusses the challenges of creating hydrogels for mechanobiology studies and how they can be used to analyze cell behavior and create regenerative therapies. Hydrogels that mimic the mechanical properties of the extracellular matrix can provide mechanical cues to cells, allowing for the study of their impact on cellular behaviors. In addition, these hydrogels can be used to create tissues in vitro for regenerative medicine applications. The article also highlights the importance of choosing appropriate cross-linking chemistry and strategies to tailor hydrogel mechanical cues, as well as techniques for characterizing hydrogels and analyzing cell behavior.
NATURE REVIEWS METHODS PRIMERS
(2022)
Article
Chemistry, Physical
Yang Sun, Sheng Yin, Jian Cui, Zhongxia Wang, Yueying Han, Ding Ma, Shuo Wang, Junhua Wu, Yi Cao, Chunping Jiang, Xiaosong Gu
Summary: Synthetic hydrogel scaffolds closely mimic liver tissue structure and provide an optimal microenvironment for hepatocyte adhesion and function. Culturing hepatocytes in these scaffolds enhances their functionality compared to traditional methods.
Article
Chemistry, Physical
Hironobu Takahashi, Haruno Wakayama, Kenichi Nagase, Tatsuya Shimizu
Summary: This study presents a multilayering approach to produce engineered human muscle tissue with sufficient contractile force. The tissue shows unidirectional contraction and can quantitatively measure drug effects.
Article
Engineering, Biomedical
Huate Li, Subramanian Sundaram, Ruifeng Hu, Lihua Lou, Francisco Sanchez, William McDonald, Arvind Agarwal, Christopher S. Chen, Thomas G. Bifano
Summary: Controlling the boundary conditions of engineered heart tissues (EHTs) in real-time allows for dynamic regulation of their contractile force. This capacity provides a new approach to study heart disease and drug toxicity screening, as well as probe tissue mechanics.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2023)
Article
Materials Science, Biomaterials
Shuyun Zhang, Xueshi Luo, Chuang Guo, Ke Huang, Shan Ding, Lihua Li, Changren Zhou, Hong Li
Summary: In this study, a novel tissue-engineered bone was developed using templated human bone mesenchymal stem cells (hBMSCs) mineralization. The resulting bone exhibited an ordered alignment of collagen fibers and a high degree of osteomimicry in terms of composition and structure. When tested in a rat cranial bone defect model, the tissue-engineered bone significantly promoted new bone formation and integration with the host bone.
BIOMATERIALS ADVANCES
(2022)
Article
Materials Science, Multidisciplinary
Simrit Safarulla, Pritisha S. Khillar, Sudarshan Kini, Amit Kumar Jaiswal
Summary: Prostate cancer bone metastasis is a complex process that occurs in the advanced stages of cancer, requiring further investigation and understanding. 3-D in vitro models provide a convenient platform for studying the development and progression of prostate cancer, aiding in the identification of potential therapeutic targets and testing treatment efficacy.
MATERIALS TODAY COMMUNICATIONS
(2021)
Article
Engineering, Biomedical
Ying Lei, Rozanne Mungai, Juanyong Li, Kristen Billiar
Summary: Heart valve disease causes significant morbidity and mortality globally, leading to a large number of heart valve replacements. Tissue engineered heart valves (TEHVs) have the potential to overcome the limitations of traditional valves, but have faced failure due to leaflet retraction. Sequential growth factor treatments using fibroblast growth factor 2 (FGF-2) and transforming growth factor beta 1 (TGF-beta 1) can minimize tissue retraction by reducing cell contractile forces and increasing extracellular matrix (ECM) stiffness. This research provides insights into growth factor-induced cell-ECM interactions and can guide the design of TEHVs with reduced retraction.
Article
Engineering, Electrical & Electronic
Irene C. Turnbull, Weibin Zhu, Francesca Stillitano, Chen-Chi Chien, Angelo Gaitas
Summary: Induced pluripotent stem cell derived-cardiomyocytes (iPSC-CMs) have great potential for various applications in cardiology research, including cell therapy and drug assessment. The study focuses on measuring contraction forces of engineered cardiac tissue using a novel micro machined sensor, and establishing a correlation between axial and vertical forces for single cell iPSC-CMs. This finding supports the use of vertical force measurements as an alternative to tissue axial force measurements.
SENSORS AND ACTUATORS A-PHYSICAL
(2021)
Article
Pharmacology & Pharmacy
Maria Koivisto, Milad Mosallaei, Tarja Toimela, Sampo Tuukkanen, Tuula Heinonen
Summary: This study developed a method for measuring cardiac contraction force using a dual-axis piezoelectric force sensor and human cell-based cardiac tissue constructs. The system was able to detect both positive and negative inotropic effects.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Viki Raz Lepsky, Sari Natan, Oren Tchaicheeyan, Avraham Kolel, Merav Zussman, Meital Zilberman, Ayelet Lesman
Summary: The study found that the addition of cells to fibrin gels slightly decreased drug release, with cellular activities affecting the release profile while forces generated by cells and matrix deformation had minimal impact on release profiles.
Article
Engineering, Biomedical
Dominik Steiner, Sophie Winkler, Stefanie Heltmann-Meyer, Vanessa T. Trossmann, Tobias Fey, Thomas Scheibel, Raymund E. Horch, Andreas Arkudas
Summary: Surgically induced angiogenesis using arteriovenous loops (AVL) is an effective method to improve vascularization of nano-fibrillary recombinant eADF4(C16) hydrogels. Additionally, incorporating RGD-tagged eADF4(C16) into hydrogels can enhance stability and vascularization, potentially optimizing silk-based biomaterials for tissue engineering applications.
Article
Biochemistry & Molecular Biology
Tanya L. Daigle, Linda Madisen, Travis A. Hage, Matthew T. Valley, Ulf Knoblich, Rylan S. Larsen, Marc M. Takeno, Lawrence Huang, Hong Gu, Rachael Larsen, Maya Mills, Alice Bosma-Moody, La' Akea Siverts, Miranda Walker, Lucas T. Graybuck, Zizhen Yao, Olivia Fong, Thuc Nghi Nguyen, Emma Garren, Garreck H. Lenz, Mariya Chavarha, Julie Pendergraft, James Harrington, Karla E. Hirokawa, Julie A. Harris, Philip R. Nicovich, Medea J. McGraw, Douglas R. Ollerenshaw, Kimberly A. Smith, Christopher A. Baker, Jonathan T. Ting, Susan M. Sunkin, Jerome Lecoq, Michael Z. Lin, Edward S. Boyden, Gabe J. Murphy, Nuno M. da Costa, Jack Waters, Lu Li, Bosiljka Tasic, Hongkui Zeng
Article
Engineering, Biomedical
Robert A. Underwood, Marcia L. Usui, Ge Zhao, Kip D. Hauch, Marc M. Takeno, Buddy D. Ratner, Andrew J. Marshall, Xuefeng Shi, John E. Olerud, Philip Fleckman
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2011)
Article
Urology & Nephrology
Wei Ling Lau, Elizabeth M. Leaf, Ming Chang Hu, Marc M. Takeno, Makoto Kuro-o, Orson W. Moe, Cecilia M. Giachelli
KIDNEY INTERNATIONAL
(2012)
Article
Multidisciplinary Sciences
Ali Shahbazi, Jeffery Kinnison, Rafael Vescovi, Ming Du, Robert Hill, Maximilian Joesch, Marc Takeno, Hongkui Zeng, Nuno Macarico da Costa, Jaime Grutzendler, Narayanan Kasthuri, Walter J. Scheirer
SCIENTIFIC REPORTS
(2018)
Correction
Multidisciplinary Sciences
Ali Shahbazi, Jeffery Kinnison, Rafael Vescovi, Ming Du, Robert Hill, Maximilian Joesch, Marc Takeno, Hongkui Zeng, Nuno Macarico da Costa, Jaime Grutzendler, Narayanan Kasthuri, Walter J. Scheirer
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Wenjing Yin, Derrick Brittain, Jay Borseth, Marie E. Scott, Derric Williams, Jedediah Perkins, Christopher S. Own, Matthew Murfitt, Russel M. Torres, Daniel Kapner, Gayathri Mahalingam, Adam Bleckert, Daniel Castelli, David Reid, Wei-Chung Allen Lee, Brett J. Graham, Marc Takeno, Daniel J. Bumbarger, Colin Farrell, R. Clay Reid, Nuno Macarico da Costa
NATURE COMMUNICATIONS
(2020)
Article
Biology
Casey M. Schneider-Mizell, Agnes L. Bodor, Forrest Collman, Derrick Brittain, Adam Bleckert, Sven Dorkenwald, Nicholas L. Turner, Thomas Macrina, Kisuk Lee, Ran Lu, Jingpeng Wu, Jun Zhuang, Anirban Nandi, Brian Hu, JoAnn Buchanan, Marc M. Takeno, Russel Torres, Gayathri Mahalingam, Daniel J. Bumbarger, Yang Li, Thomas Chartrand, Nico Kemnitz, William M. Silversmith, Dodam Ih, Jonathan Zung, Aleksandar Zlateski, Ignacio Tartavull, Sergiy Popovych, William Wong, Manuel Castro, Chris S. Jordan, Emmanouil Froudarakis, Lynne Becker, Shelby Suckow, Jacob Reimer, Andreas S. Tolias, Costas A. Anastassiou, H. Sebastian Seung, R. Clay Reid, Nuno Macarico da Costa
Summary: This study used electron microscopy and functional imaging to investigate the connectivity of chandelier cells in the mouse visual cortex, finding that synapse number is highly variable across the population and correlated with structural features of target neurons. Biophysical simulations demonstrated the effectiveness of axo-axonic inhibition in controlling excitatory output, especially when excitation and inhibition are co-active. In vivo chandelier cell activity was found to be highly correlated with pupil dilation, suggesting that chandelier cells provide a circuit-wide signal adjusted relative to target neuron properties.
Article
Biochemistry & Molecular Biology
Nicholas L. Turner, Thomas Macrina, J. Alexander Bae, Runzhe Yang, Alyssa M. Wilson, Casey Schneider-Mizell, Kisuk Lee, Ran Lu, Jingpeng Wu, Agnes L. Bodor, Adam A. Bleckert, Derrick Brittain, Emmanouil Froudarakis, Sven Dorkenwald, Forrest Collman, Nico Kemnitz, Dodam Ih, William M. Silversmith, Jonathan Zung, Aleksandar Zlateski, Ignacio Tartavull, Szi-chieh Yu, Sergiy Popovych, Shang Mu, William Wong, Chris S. Jordan, Manuel Castro, JoAnn Buchanan, Daniel J. Bumbarger, Marc Takeno, Russel Torres, Gayathri Mahalingam, Leila Elabbady, Yang Li, Erick Cobos, Pengcheng Zhou, Shelby Suckow, Lynne Becker, Liam Paninski, Franck Polleux, Jacob Reimer, Andreas S. Tolias, R. Clay Reid, Nuno Macarico da Costa, H. Sebastian Seung
Summary: This article describes a model reconstructed from electron microscopic images of mouse primary visual cortex, and investigates the relationship between cell organization and function. The study suggests that the connectivity pattern of pyramidal cells is associated with their visual response strength and reliability.
Article
Cell Biology
Stephanie K. Bonney, Vanessa Coelho-Santos, Sheng-Fu Huang, Marc Takeno, Joergen Kornfeld, Annika Keller, Andy Y. Shih
Summary: This article presents a meta-analysis of multiple data sets to explore the ultrastructural features of the cerebrovasculature. The analysis reveals diverse cell types and subcellular details, providing important insights into the functions of blood-brain barrier, blood flow control, brain clearance, and bioenergetics. The article also discusses the limitations and possible improvements of vascular segmentation and analysis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Gayathri Mahalingam, Russel Torres, Daniel Kapner, Eric T. Trautman, Tim Fliss, Shamishtaa Seshamani, Eric Perlman, Rob Young, Samuel Kinn, JoAnn Buchanan, Marc M. Takeno, Wenjing Yin, Daniel J. Bumbarger, Ryder P. Gwinn, Julie Nyhus, Ed Lein, Steven J. Smith, R. Clay Reid, Khaled A. Khairy, Stephan Saalfeld, Forrest Collman, Nuno Macarico da Costa
Summary: Serial-section electron microscopy is a high-resolution method for studying biological samples, with applications in reconstructing neural wiring diagrams. We present ASAP, a software pipeline that can handle large datasets in a distributed computational environment, offering high throughput and modular design.
Article
Multidisciplinary Sciences
JoAnn Buchanan, Leila Elabbady, Forrest Collman, Nikolas L. Jorstad, Trygve E. Bakken, Carolyn Ott, Jenna Glatzer, Adam A. Bleckert, Agnes L. Bodor, Derrick Brittain, Daniel J. Bumbarger, Gayathri Mahalingam, Sharmishtaa Seshamani, Casey Schneider-Mizell, Marc M. Takeno, Russel Torres, Wenjing Yin, Rebecca D. Hodge, Manuel Castro, Sven Dorkenwald, Dodam Ih, Chris S. Jordan, Nico Kemnitz, Kisuk Lee, Ran Lu, Thomas Macrina, Shang Mu, Sergiy Popovych, William M. Silversmith, Ignacio Tartavull, Nicholas L. Turner, Alyssa M. Wilson, William Wong, Jingpeng Wu, Aleksandar Zlateski, Jonathan Zung, Jennifer Lippincott-Schwartz, Ed S. Lein, H. Sebastian Seung, Dwight E. Bergles, R. Clay Reid, Nuno Macarico da Costa
Summary: Neurons in the developing brain undergo structural refinement through the engulfment and degradation of axonal branches and synapses by glial cells. In the mouse visual cortex, oligodendrocyte precursor cells (OPCs) were found to surround small branches of axons and engage in axon pruning. Compared to microglia, OPCs were more abundant in phagosomes and phagolysosomes, suggesting their substantial contribution to neuronal circuit refinement during cortical development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Sven Dorkenwald, Nicholas L. Turner, Thomas Macrina, Kisuk Lee, Ran Lu, Jingpeng Wu, Agnes L. Bodor, Adam A. Bleckert, Derrick Brittain, Nico Kemnitz, William M. Silversmith, Dodam Ih, Jonathan Zung, Aleksandar Zlateski, Ignacio Tartavull, Szi-Chieh Yu, Sergiy Popovych, William Wong, Manuel Castro, Chris S. Jordan, Alyssa M. Wilson, Emmanouil Froudarakis, JoAnn Buchanan, Marc M. Takeno, Russel Torres, Gayathri Mahalingam, Forrest Collman, Casey M. Schneider-Mizell, Daniel J. Bumbarger, Yang Li, Lynne Becker, Shelby Suckow, Jacob Reimer, Andreas S. Tolias, Nuno Macarico da Costa, R. Clay Reid, H. Sebastian Seung
Summary: Learning from experience depends on changes in neuronal connections. In this study, the authors present a large map of connectivity between cortical neurons in the mouse primary visual cortex and use it to identify constraints on learning algorithms used by the cortex. They find that synapse size, specifically between layer 2/3 pyramidal cells, can be modeled as a combination of a binary variable and an analog variable drawn from a log-normal distribution. The binary variables of two synapses are highly correlated, while the analog variables are not.
Proceedings Paper
Engineering, Biomedical
Gayathri Mahalingam, Tong Jiao, Casey Schneider-Mizell, Agnes Bodor, Russel Torres, Marc Takeno, JoAnn Buchanan, Daniel Bumbarger, Wenjing Yin, Derrick Brittain, Clay Reid, Nuno Da Costa
Summary: This paper proposes a zero-shot learning approach for pixel-based segmentation of resin and film regions in unknown datasets. By combining a U-Net segmentation network with an unsupervised image-to-image translation method, the segmentation accuracy is significantly improved. This approach automates the detection of anomalies, facilitating the reconstruction of dense neural wiring diagrams in the brain.
2022 IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (IEEE ISBI 2022)
(2022)
Article
Biology
Jun Zhuang, Quanxin Wang, Marc Takeno, Jack Waters