Article
Pharmacology & Pharmacy
Martin Philipp Dieterle, Thorsten Steinberg, Pascal Tomakidi, Jiri Nohava, Kirstin Vach, Simon Daniel Schulz, Elmar Hellwig, Susanne Proksch
Summary: This study developed a new nonwoven scaffold for periodontal tissue engineering. Nonwovens of 16% gelatin/5% hydroxyapatite with additional porosity were produced by electrospinning and in situ cross-linking. The results showed that the scaffold efficiently supported the adhesion and survival of human mesenchymal stem cells (hMSCs) and periodontal ligament fibroblasts (PDLFs), indicating its potential applications.
Article
Polymer Science
Timur Kh Tenchurin, Alla Rodina, Vladimir P. Saprykin, Lada Gorshkova, Alexey A. Mikhutkin, Roman A. Kamyshinsky, Dmitry S. Yakovlev, Alexander L. Vasiliev, Sergey N. Chvalun, Timofey E. Grigoriev
Summary: The 3D reconstruction of fibrous poly-L/L-lactide scaffolds with different thicknesses showed that the density of fibers on the side adjacent to the electrode is higher, which can affect cell diffusion. Stem cells cultured in a 600 μm-thick scaffold formed colonies and showed higher cell proliferation and growth factor release compared to a 100 μm-thick scaffold. In vivo experiments demonstrated that the cells seeded onto the 600 μm-thick scaffold promoted neovascularization.
Article
Materials Science, Multidisciplinary
Martina Martinkova, Berenika Hausnerova, Jakub Huba, Tomas Martinek, Simona Kacerova, Vera Kasparkova, Petr Humpolicek
Summary: Alumina-based scaffolds prepared by powder injection molding can be used for bone graft preparation. The final architecture of the scaffolds can be controlled by the size and volume ratio of the powder space holder. The cell-instructive property of alumina can be enhanced by coating it with electrically-conducting polyaniline or polyaniline/biopolymer films. The cytocompatibility of the functionalized scaffolds was confirmed, showing their potential application.
MATERIALS & DESIGN
(2022)
Review
Engineering, Biomedical
Ping Xia, Yongxiang Luo
Summary: Vascularization is a key event in tissue engineering, with the architecture features of pores in scaffolds playing important roles by facilitating cell migration, nutrient, and oxygen diffusion for promoting new tissue formation and vascularization. The pore parameters, including size, interconnectivity, distribution, shape, and surface topography, are crucial factors affecting cells response and vessel formation.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Egor Morokov, Anna Zykova, Polina Tyubaeva
Summary: Fibrous scaffolds are essential materials in tissue engineering, but assessing their mechanical behavior in wet environments is challenging. By combining high-frequency acoustic microscopy with a mechanical testing machine, high-resolution imaging of the internal microstructure of scaffolds under tension in water-filled specimens is possible. This method provides a foundation for future experimental setups to characterize tissue-engineered constructs in conditions closer to those in living organisms.
ADVANCED ENGINEERING MATERIALS
(2021)
Article
Engineering, Biomedical
Xavier Barcelo, Kian F. Eichholz, Ines F. Goncalves, Orquidea Garcia, Daniel J. Kelly
Summary: This study demonstrates a biofabrication strategy using inkjet bioprinting to engineer fibrocartilage tissues with user-defined collagen architectures. The scaffold architecture was found to influence the spatial organization of the collagen network, and higher aspect ratios supported the formation of structurally anisotropic tissues. This technology could potentially provide new treatment options for damaged and diseased meniscal tissue.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Environmental
Daniel J. Vandenheuvel, Brenna L. Devlin, Pascal R. Buenzli, Maria A. Woodruff, Matthew J. Simpson
Summary: Understanding the influence of pore geometry on tissue growth is crucial for tissue engineering. In this study, we investigated the effects of square-shaped and non-square pores with wave-shaped boundaries on tissue growth using osteoblastic cells. Our results suggest that cell migration and cell proliferation rates are not significantly influenced by pore geometries, indicating that observed curvature effects on tissue growth are mainly due to space availability. This finding provides a basis for estimating parameters and predicting tissue growth in more realistic and complex pores using simple square-shaped pores.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Yanan Wang, Eunkyoung Shim, Nanfei He, Behnam Pourdeyhimi, Wei Gao
Summary: The theoretical model for nonwoven-based TENGs provides insight into the correlation between triboelectric output and structural parameters, with successful validation through sample preparation and analysis. The model demonstrates good predictive power and universality in predicting the performance of meltblown nonwoven-based TENGs.
ADVANCED MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Afroditi Nanou, Ivan Lorenzo-Moldero, Kyriakos D. Gazouleas, Barbara Cortese, Lorenzo Moroni
Summary: Cancer biology research is shifting towards innovative in vitro 3D culture models to better mimic in vivo cancer biology. This study evaluated a 3D engineered system using porous scaffolds with two different porosities and compared it to conventional 2D cell culture. The results showed that static seeding was the optimal method and cells cultured in 3D scaffolds exhibited a more dormant phenotype.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Huixing Liang, Ziming Wang, Junsong Wu, Xiang Li, D. T. Semirumi
Summary: This study focuses on using PVA and gelatin as biocompatible and biodegradable polymers, along with Arabian gum-HA, to create porous biological tissue with improved mechanical properties. The addition of gum reduced the percentage of porosity, increased tensile strength, and improved the tissue's resistance to destruction.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Polymer Science
Gustavo A. Rico-Llanos, Sara Borrego-Gonzalez, Miguelangel Moncayo-Donoso, Jose Becerra, Rick Visser
Summary: Collagen type I is widely used in bone tissue engineering due to its excellent biocompatibility and osteoconductivity, but it also has drawbacks such as high biodegradability and low mechanical strength. Recent efforts have been focused on designing and producing collagen implants to enhance bone regeneration.
Article
Polymer Science
Thamires Santos da Silva, Bianca de Oliveira Horvath-Pereira, Leandro Norberto da Silva-Junior, Joao Victor Barbosa Tenorio Fireman, Michel Mattar, Marcilio Felix, Rogerio Leone Buchaim, Ana Claudia Oliveira Carreira, Maria Angelica Miglino, Marcelo Melo Soares
Summary: This study characterizes the properties of GO/PLLA-printed scaffolds and demonstrates their potential applications in fields such as bone tissue engineering and implants. The research findings indicate that the GO/PLLA nanocomposites are not cytotoxic and the printed scaffolds exhibit good reproducibility.
Article
Engineering, Biomedical
Rui Zhang, Junqun Jiang, Yaling Yu, Fangfang Wang, Niuniu Gao, Yingjie Zhou, Xinlong Wan, Zhibin Wang, Peng Wei, Jin Mei
Summary: Chronic kidney disease is a major global public health problem, with renal fibrosis being a common pathological process in chronic renal failure. Research indicates that renal fibrosis decellularized scaffolds have the potential to serve as a model for cellular mechanisms of tissue fibrosis or donors for tissue engineering. Analysis through histology, proteomics, and ELISA showed changes in the structure and components of fibrotic tissue. The fibrosis model induced by adenine-fed demonstrated changes in the fibrotic scaffold's structure and protein expression, but cytokines associated with renal regeneration post-injury were maintained, indicating potential for tissue engineering.
BIOACTIVE MATERIALS
(2021)
Article
Engineering, Biomedical
Mariya M. Mikhailova, Konstantin Sydoruk, Lubov Davydova, Evgeniy Yastremsky, Sergey N. Chvalun, Vladimir G. Debabov, Vladimir G. Bogush, Andrey A. Panteleyev
Summary: The study assessed the suitability of electrospun recombinant spidroin-based matrices for cultivation of the mouse aorta and dorsal root ganglia (DRG) explants, and quantified the effects of matrix topography on both types of tissues. The results showed that the RS-based matrices effectively supported aortic explants survival and sprouting, and matrices with parallel fibers highly oriented cell migration. The findings demonstrate that electrospun RS matrices are highly suitable for culturing both nerve and vascular cells, and studying the effects of matrix topography on cell migration.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2022)
Article
Biotechnology & Applied Microbiology
Elisa Capuana, Simona Campora, Giorgio Catanzaro, Francesco Lopresti, Gioacchino Conoscenti, Giulio Ghersi, Vincenzo La Carrubba, Valerio Brucato, Francesco Carfi Pavia
Summary: Perfusion of flow during cell culture promotes cell proliferation and enhances cellular activity. Numerical simulations and micro-computed tomography were used to evaluate the effects of operating parameters of a custom-made bioreactor and the porous microstructure of scaffolds on fluid flow and cell viability. The results showed a uniform distribution of liquid velocities and confirmed the importance of flow uniformity for successful perfusion cultures.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Biomedical
Philippa Bowland, Raelene M. Cowie, Eileen Ingham, John Fisher, Louise M. Jennings
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART H-JOURNAL OF ENGINEERING IN MEDICINE
(2020)
Article
Construction & Building Technology
E. A. Essah, S. J. Russell, S. D. Waring, J. Ferguson, C. Williams, K. Walsh, S. Dyer, R. Raynor
BUILDING RESEARCH AND INFORMATION
(2020)
Article
Biophysics
Wen-Hao Dong, Jia-Xu Liu, Xiao-Ju Mou, Guo-Sai Liu, Xiao-Wei Huang, Xu Yan, Xin Ning, Stephen J. Russell, Yun-Ze Long
COLLOIDS AND SURFACES B-BIOINTERFACES
(2020)
Article
Infectious Diseases
Amy Wheldrake, Estelle Guillemois, Hamidreza Arouni, Vera Chetty, Stephen J. Russell
Summary: Mechanical damage, particularly snagging, tearing, and abrasion, is the primary cause of the loss of physical integrity in LLINs. New specifications and minimum standards for seam construction are needed to improve the resistance of LLIN products to mechanical damage during normal use.
Article
Infectious Diseases
Amy Wheldrake, Estelle Guillemois, Vera Chetty, Albert Kilian, Stephen J. Russell
Summary: The study developed algorithms and conducted laboratory textile testing to determine the RD value of any LLIN product prior to distribution. Only six out of sixteen LLINs achieved an RD value above 50, suggesting a need for product innovation to increase resistance to damage. LLINs with higher RD values were associated with lower hole damage in the field.
Article
Infectious Diseases
Amy Wheldrake, Estelle Guillemois, Hamidreza Arouni, Vera Chetty, Stephen J. Russell
Summary: LLINs are susceptible to forming holes quickly during use, with mechanical damage being responsible for the majority. The resistance to damage varies based on fabric design specifications, rather than polymer type. A new suite of testing methods allows for assessment and specification of LLIN performance prior to distribution, establishing minimum performance standards.
Article
Infectious Diseases
Albert Kilian, Emmanuel Obi, Paul Mansiangi, Ana Paula Abilio, Khamis Ameir Haji, Estelle Guillemois, Vera Chetty, Amy Wheldrake, Sean Blaufuss, Bolanje Olapeju, Stella Babalola, Stephen J. Russell, Hannah Koenker
Summary: This study explores the usefulness of the resistance to damage (RD) score in predicting the physical durability of LLIN products. The results suggest that a 10-point increase in RD score can reduce the risk of failure by 36% and extend the useful life of LLINs by 7 months.
Article
Biotechnology & Applied Microbiology
Reem El-Gendy, Sarah Junaid, Stephen K. L. Lam, Karen M. Elson, Joanne L. Tipper, Richard M. Hall, Eileen Ingham, Jennifer Kirkham
Summary: This study established a porcine tooth in situ organ culture system successfully maintained over 4 days in vitro. The viability, maintenance of structure, and sterility of the model were confirmed through various assays and analysis methods, demonstrating its potential for future research in periodontal regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Engineering, Biomedical
Malachy Maher, Miguel Castilho, Zhilian Yue, Veronica Glattauer, Timothy C. Hughes, John A. M. Ramshaw, Gordon G. Wallace
Summary: Hard tissue engineering has evolved rapidly, with no gold standard approach to engineering and regenerating hard tissue substitutes yet. One unique strategy is the in vitro mineralisation of collagen templates, which relies on the collagen template and mineralisation strategy for success.
ACTA BIOMATERIALIA
(2021)
Article
Materials Science, Textiles
Andrea L. Wilde, David L. J. Alexander, Anthony P. Pierlot, Ronald Denning, Menghe Miao
Summary: The research developed a simple empirical model to predict the density of carbon fibers and analyzed the relative cyclization index and density. After experimental verification, the model showed good agreement with the predictions.
FIBERS AND POLYMERS
(2021)
Article
Materials Science, Multidisciplinary
Ke Du, Johan Basuki, Veronica Glattauer, Caroline Mesnard, Anh Tuan Nguyen, David L. J. Alexander, Timothy C. Hughes
Summary: The developed silicone thiourea resins improve the elasticity of 3D-printed silicones by increasing dynamic noncovalent intermolecular interactions between polymer chains through the introduction of thiourea groups. A molecular design strategy was implemented to adjust the ratio of thiourea segments using oligomeric and polymeric PDMS units. The cured silicone elastomer displayed a maximum elongation of up to 1000% under tensile load and excellent cyclic compression durability, allowing for the 3D printing of PDMS-based objects with complex microarchitectures, high resolution, excellent surface finishing, and low cytotoxicity, with potential applications in medical devices, wearable devices, and soft robotics.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Engineering, Biomedical
Ashley Ward, Debora Morgante, John Fisher, Eileen Ingham, Jennifer Southgate
Summary: Bladder acellular matrix has potential applications in reconstructive surgeries, but the decellularization process is complex. This study used a mechanical engineering approach to design a laser-printed flat-bed apparatus for reproducible and full-thickness bladder tissue decellularization.
BIOMEDICAL MATERIALS
(2021)
Article
Chemistry, Medicinal
Malachy Maher, Veronica Glattauer, Carmine Onofrillo, Serena Duchi, Zhilian Yue, Timothy C. Hughes, John A. M. Ramshaw, Gordon G. Wallace
Summary: This study compares the potential and performance differences of collagen from marine and porcine sources in tissue engineering. Collagen derived from marine sources has lower risk of disease transfer and is more compatible with religious and ethical groups. Although it has inferior mechanical properties and is prone to degradation, marine-derived collagen is still considered a promising biomaterial.
Article
Polymer Science
Malachy Kevin Maher, Jacinta F. White, Veronica Glattauer, Zhilian Yue, Timothy C. Hughes, John A. M. Ramshaw, Gordon G. Wallace
Summary: Collagen, as the most abundant protein in the extracellular matrix, has been extensively studied in tissue engineering and regenerative medicine. Collagen type I is commonly used in laboratory studies. The structure of the collagen fibril network affects cellular proliferation, differentiation, and the modulus of hydrogels. This study compared the network-forming properties of two forms of collagen and investigated the impact of methacrylation on the crosslinking and 3D printing suitability. The results showed that the presence of telopeptides facilitated fibril formation, but methacrylation reduced the self-assembly potential. Crosslinking improved the fibril-like network structure.
Article
Engineering, Biomedical
Marcin Kotlarz, Ana M. Ferreira, Piergiorgio Gentile, Stephen J. Russell, Kenneth Dalgarno
Summary: Composites offer the option of combining the benefits of their constituents to achieve unique material properties in tissue engineering applications. In this study, a droplet-based bioprinting system called ReJI was used to integrate a cell-laden hydrogel with a microfibrous mesh. The cell-hydrogel-fibre composites showed high cell viability and promoted cell-cell and cell-biomaterial interactions. The fibrous component of the composites exhibited high swelling properties and quick release of calcium ions.
BIO-DESIGN AND MANUFACTURING
(2022)