Review
Chemistry, Multidisciplinary
Hamidreza Tolabi, Niyousha Davari, Mehran Khajehmohammadi, Haniyeh Malektaj, Katayoun Nazemi, Samaneh Vahedi, Behafarid Ghalandari, Rui L. Reis, Farnaz Ghorbani, Joaquim Miguel Oliveira
Summary: Cartilage degeneration is a major cause of disability and pain worldwide, but current treatments have limited success in the long term. The convergence of tissue engineering and microfabrication principles offers advanced microfluidic technologies as alternatives. This review summarizes the developments in microfluidic hydrogel-based scaffolds for cartilage regeneration and discusses the advantages over traditional hydrogels. It also presents the technologies for fabricating microfluidic scaffolds and chips, and highlights the preclinical and clinical applications and challenges in developing microfluidic systems for cartilage repair.
ADVANCED MATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Marcin Szustak, Edyta Gendaszewska-Darmach
Summary: Nanocellulose is a promising biocompatible biomaterial with good mechanical properties for use as a scaffold in cartilage tissue engineering. The limited regenerative capacity of cartilage due to its lack of vascularization and sparsely distributed chondrocytes poses challenges for cell infiltration, making nanocellulose scaffolds a potential solution. The scaffolds have been shown to induce stem cell differentiation into chondrocyte phenotypes, offering opportunities for cartilage tissue development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Kenny A. van Kampen, Elena Olaret, Izabela-Cristina Stancu, Daniela F. Duarte Campos, Horst Fischer, Carlos Mota, Lorenzo Moroni
Summary: The main function of articular cartilage is to provide a low friction surface and protect the underlying bone. In this study, a scaffold design based on hypotrochoidal curves was developed and compared to a regular woodpile design. The hypotrochoidal design showed improved mechanical properties and enhanced extracellular matrix composition, indicating its potential for cartilage tissue engineering.
MATERIALS TODAY BIO
(2023)
Article
Polymer Science
Rachel Cordeiro, Rui D. D. Alvites, Ana C. C. Sousa, Bruna Lopes, Patricia Sousa, Ana C. C. Mauricio, Nuno Alves, Carla Moura
Summary: Osteoarthritis is a prevalent disease causing cartilage loss, and tissue engineering with different cellulose incorporated poly(epsilon-caprolactone) (PCL) scaffolds shows promise for its repair. The study manufactured and characterized PCL scaffolds with various percentages of commercial cellulose (microcrystalline and methyl cellulose) or cellulose from agro-industrial residues (corncob). The results indicate that cellulose incorporation promotes cellular adhesion/proliferation, and methyl cellulose scaffolds demonstrate compressive properties closer to native cartilaginous tissue.
Review
Chemistry, Applied
Nazanin Amiryaghoubi, Marziyeh Fathi, Jaleh Barar, Yadollah Omidi
Summary: Tissue engineering involves the use of safe scaffolds, bioactive substances, and live cells to regenerate damaged tissue/organs. The success of tissue regeneration depends on the properties of the scaffold, integrity of the cells, and the biological setting. Advanced biomimetic hydrogels are promising for bone and cartilage tissue engineering due to their unique features and ability to accommodate cells and necessary substances for regeneration.
REACTIVE & FUNCTIONAL POLYMERS
(2022)
Article
Engineering, Biomedical
Xianggang Wang, Zuhao Li, Jiaqi Liu, Chenyu Wang, Haotian Bai, Xiujie Zhu, Hui Wang, Zhonghan Wang, He Liu, Jincheng Wang
Summary: This study presents the development of a bioinspired scaffold using 3D printing technology for growth plate repair. The scaffold, with surface modification and cell loading, successfully rebuilds the structure of the growth plate and reduces limb abnormalities.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Xianggang Wang, Zuhao Li, Jiaqi Liu, Chenyu Wang, Haotian Bai, Xiujie Zhu, Hui Wang, Zhonghan Wang, He Liu, Jincheng Wang
Summary: The growth plate plays a crucial role in longitudinal bone growth, and injuries to the growth plate can result in limb abnormalities in children. This study developed a bioinspired stratified scaffold using 3D printing technology to promote the reconstruction of the growth plate structure and reduce limb abnormalities.
MATERIALS TODAY BIO
(2023)
Review
Polymer Science
Md Mohosin Rana, Hector de la Hoz Siegler
Summary: PNIPAm has the potential for tissue engineering applications, but challenges such as mechanical strength and biocompatibility still need to be addressed. Researchers have explored various design variables to optimize the performance of PNIPAm-based scaffolds for guiding cartilage tissue regeneration.
Article
Biochemistry & Molecular Biology
Yasamin Alikhasi Amnieh, Sepideh Ghadirian, Nayereh Mohammadi, Mohammad Shadkhast, Saeed Karbasi
Summary: In this study, chitosan nanoparticles (Cs) were synthesized and incorporated into polyhydroxy butyrate (PHB) electrospun scaffolds for cartilage tissue engineering. The mechanical properties, hydrophilicity, and fiber diameter of the PHB scaffolds with varying concentrations of Cs nanoparticles were evaluated. The scaffold containing 1 wt% Cs nanoparticles (P-1Cs) showed optimal properties with increased strength and elongation at break compared to pure PHB. Crystallinity, degradation, and cell compatibility were also evaluated, and Cs nanoparticles were found to improve cell proliferation on the scaffold.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Polymer Science
Liang Lu, Xifu Shang, Bin Liu, Weijian Chen, Yu Zhang, Shuyun Liu, Xiang Sui, Aiyuan Wang, Quanyi Guo
Summary: This paper explores the application value of tissue engineering in vitro for the construction of artificial cartilage. By seeding healthy porcine chondrocytes on articular cartilage extracellular matrix (ACECM) scaffolds, it was found that the cells performed well in terms of adhesion and growth on the scaffolds, and they secreted collagen type II. Moreover, the porcine ACECM scaffolds showed good biocompatibility and could be used for xenotransplantation without significant immune inflammatory response. Therefore, ACECM-oriented scaffold is an ideal natural biomaterial for cartilage tissue engineering.
Article
Biotechnology & Applied Microbiology
Mantas Malinauskas, Lina Jankauskaite, Lauryna Aukstikalne, Lauryna Dabasinskaite, Augustinas Rimkunas, Tomas Mickevicius, Alius Pockevicius, Edvinas Krugly, Dainius Martuzevicius, Darius Ciuzas, Odeta Baniukaitiene, Arvydas Usas
Summary: Polycaprolactone (PCL) is a suitable material for cartilage tissue engineering due to its mechanical strength, low immunogenicity, elasticity, and biodegradability. Enhancing the hydrophilicity of PCL scaffolds is necessary, and in this study, ozone treatment and TGF beta 3 loading were used to improve the properties of electrospun bilayer PCL scaffolds. In vitro and in vivo evaluations showed that the ozone-treated scaffold loaded with TGF beta 3 and rMDSCs demonstrated better chondrogenic potential and cartilage regeneration outcomes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Zohre Montaseri, Samira Sadat Abolmaali, Ali Mohammad Tamaddon, Fakhrossadat Farvadi
Summary: Cartilage tissue engineering requires an appropriate microenvironment for chondrocyte proliferation. Composite silk fibroin hydrogels have shown strong potential and advantages for cartilage regeneration.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Frederico Barbosa, Frederico Castelo Ferreira, Joao Carlos Silva
Summary: Osteochondral tissue engineering is a promising strategy for treating diseases such as osteoarthritis. However, current research focuses mainly on the structural and mechanical properties of the tissue, while neglecting its piezoelectric properties. This review summarizes the recent advances in the application of piezoelectric electrospun scaffolds for replacing damaged osteochondral tissue and promoting its regeneration, and discusses the current challenges and future perspectives.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Rui Zhang, Junqun Jiang, Yaling Yu, Fangfang Wang, Niuniu Gao, Yingjie Zhou, Xinlong Wan, Zhibin Wang, Peng Wei, Jin Mei
Summary: Chronic kidney disease is a major global public health problem, with renal fibrosis being a common pathological process in chronic renal failure. Research indicates that renal fibrosis decellularized scaffolds have the potential to serve as a model for cellular mechanisms of tissue fibrosis or donors for tissue engineering. Analysis through histology, proteomics, and ELISA showed changes in the structure and components of fibrotic tissue. The fibrosis model induced by adenine-fed demonstrated changes in the fibrotic scaffold's structure and protein expression, but cytokines associated with renal regeneration post-injury were maintained, indicating potential for tissue engineering.
BIOACTIVE MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Siyu Wu, Wenlai Guo, Rui Li, Xi Zhang, Wenrui Qu
Summary: Articular cartilage has limited self-regeneration ability, but platelet derivatives combined with biomaterials provide an economical and effective treatment method for cartilage tissue engineering, with mechanical support and localized release of bioactive molecules.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Weikun Meng, Ana Rey-Rico, Mickael Claudel, Gertrud Schmitt, Susanne Speicher-Mentges, Francoise Pons, Luc Lebeau, Jagadeesh K. Venkatesan, Magali Cucchiarini
Article
Pharmacology & Pharmacy
Jagadeesh K. Venkatesan, Weikun Meng, Ana Rey-Rico, Gertrud Schmitt, Susanne Speicher-Mentges, Celine Falentin-Daudre, Amelie Leroux, Henning Madry, Veronique Migonney, Magali Cucchiarini
Article
Pharmacology & Pharmacy
Fernando Alvarez-Rivera, Ana Rey-Rico, Jagadeesh K. Venkatesan, Luis Diaz-Gomez, Magali Cucchiarini, Angel Concheiro, Carmen Alvarez-Lorenzo
Article
Orthopedics
Yannik P. Morscheid, Jagadeesh K. Venkatesan, Gertrud Schmitt, Patrick Orth, David Zurakowski, Susanne Speicher-Mentges, Michael D. Menger, Matthias W. Laschke, Magali Cucchiarini, Henning Madry
Summary: The study demonstrated the potential of direct rAAV-mediated FGF-2 gene therapy to enhance osteochondral repair in a large animal model over time in vivo, leading to increased subchondral bone formation and improved cartilage repair.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Jagadeesh K. Venkatesan, Xiaoyu Cai, Weikun Meng, Ana Rey-Rico, Gertrud Schmitt, Susanne Speicher-Mentges, Celine Falentin-Daudre, Amelie Leroux, Henning Madry, Veronique Migonney, Magali Cucchiarini
Summary: This study investigated the potential of transferring a recombinant adeno-associated virus (rAAV) vector carrying a sequence for transforming growth factor beta (TGF-beta) in chondrogenically competent bone marrow aspirates using poly(e-caprolactone) (PCL) films functionalized with poly(sodium styrene sulfonate) (pNaSS). The results showed that the PCL film-assisted rAAV TGF-beta gene transfer can enhance reparative potential in cartilage lesions, supporting improved cartilage repair in future approaches.
HUMAN GENE THERAPY
(2021)
Article
Chemistry, Multidisciplinary
Johanna Maihoefer, Henning Madry, Ana Rey-Rico, Jagadeesh K. Venkatesan, Lars Goebel, Gertrud Schmitt, Susanne Speicher-Mentges, Xiaoyu Cai, Weikun Meng, David Zurakowski, Michael D. Menger, Matthias W. Laschke, Magali Cucchiarini
Summary: In this study, gene therapy using an alginate hydrogel as a carrier for the delivery of the IGF-I gene was found to enhance cartilage repair in a minipig model. The sustained overexpression of IGF-I significantly improved parameters of cartilage repair and reduced perifocal osteoarthritis and inflammation after one year of treatment, demonstrating the potential clinical application of biomaterial-guided gene therapy for promoting cartilage repair and protecting against OA.
ADVANCED MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Emilie Velot, Henning Madry, Jagadeesh K. Venkatesan, Arnaud Bianchi, Magali Cucchiarini
Summary: Extracellular vesicles (EVs) are biological particles released from cells that act as messengers for cell-to-cell communication. They have a membrane with a lipid bilayer, but lack a nucleus and consequently are unable to replicate. Researchers focus on their biological effects rather than their specific origins.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Materials Science, Biomaterials
M. Isabel Rial-Hermida, Ana Rey-Rico, Barbara Blanco-Fernandez, Natalia Carballo-Pedrares, Eimear M. Byrne, Joao F. Mano
Summary: Polysaccharides have been widely used in the development of delivery systems for biotherapeutics in the pharmaceutical and biomedical fields, due to their renewable nature, biocompatibility, and ease of chemical modification.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Liliana Barravecchia, Arturo Blanco-Gomez, Iago Neira, Raminta Skackauskaite, Alejandro Vila, Ana Rey-Rico, Carlos Peinador, Marcos D. Garcia
Summary: We present a new class of pH-responsive viologen analogues called vermellogens, which are synthesized efficiently in aqueous media using hydrazone exchange reactions. The vermellogens show significant pH-responsivity and the parent analogue, N,N'-dimethylated vermellogen, exhibits anion-recognition abilities and non-toxicity. The host-guest chemistry of the vermellogens with macrocyclic receptors has been studied experimentally and theoretically, showing similar behavior to a known herbicide but with different redox reactive capabilities.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Pharmacology & Pharmacy
Natalia Carballo-Pedrares, Clara Sanjurjo-Rodriguez, Jose Senaris, Silvia Diaz-Prado, Ana Rey-Rico
Summary: This study utilized new non-viral carriers based on niosomes to promote effective chondrogenesis of human mesenchymal stem cells, showing promise for gene therapy approaches focused on cartilage repair.
Review
Materials Science, Multidisciplinary
J. K. Venkatesan, A. Rey-Rico, W. Meng, X. Cai, F. Pons, L. Lebeau, V Migonney, H. Madry, M. Cucchiarini
Summary: Biomaterial-assisted gene therapy shows promise in delivering candidate gene sequences to sites of tissue damage in a spatially and temporally controlled manner, potentially offering an effective and safe treatment option for musculoskeletal disorders in the future.
MATERIALS TODAY ADVANCES
(2021)
