Article
Pharmacology & Pharmacy
Eunmi Ban, Aeri Kim
Summary: Coacervation is a liquid-liquid phase separation that occurs in solutions of macromolecules through self-assembly or electrostatic interactions. These coacervates, composed of biocompatible macromolecules, are actively being studied as platforms for encapsulating and delivering biomolecules. Despite their advantages, challenges remain in scaling up manufacturing methods for preclinical and clinical evaluations.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Biochemistry & Molecular Biology
Fatima Ijaz, Hafiz Muhammad Tahir, Shaukat Ali, Aamir Ali, Hooria Ashraf Khan, Ayesha Muzamil, Hafiz Hamid Manzoor, Kunza Abdul Qayyum
Summary: The need for biocompatible drug carriers has increased, and researchers are interested in developing diverse and complex biomaterial-based carriers. Hydrogels are beneficial drug carriers due to their tunable structure and ability to release drugs at target sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Faiza Ahsan, Muhammad Yar, Adnan Gulzar, Khurshid Ayub
Summary: Conventional drug delivery systems face challenges of poor absorption and bioavailability at the target site. 2D porous nanostructures, such as C2N, can offer improved drug delivery systems by providing suitable cavities for drug molecules. This study explores the potential of C2N surface as a drug delivery system for nitrosourea and fluorouracil. The results show that both drugs have good adsorption on the C2N surface and C2N is a better carrier for nitrosourea compared to fluorouracil. Molecular dynamics simulations reveal that slightly elevated temperature can cause off-loading of the drugs at the target cancer cells. Overall, C2N is found to be a better carrier for these drugs compared to other surfaces.
JOURNAL OF NANOSTRUCTURE IN CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yuxuan Ge, Fan Rong, Wei Li, Yin Wang
Summary: This review focuses on the recent advances in biomolecular-based H2S delivery systems, which combine H2S donors with biomolecular vectors such as polysaccharide, peptide, and protein. The review discusses their basic structures, building strategies, therapeutic applications, and the advantages and development potentials provided by biomolecules.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Engineering, Biomedical
Hui Zhu, Kai Zheng, Aldo R. Boccaccini
Summary: In summary, mesoporous silica-based materials, especially MBGs, are promising for biomedical applications due to their bioactivity, biocompatibility, tunable composition, and controllable nanoporosity. Research has shown that MBGs can be doped with therapeutic ions and loaded with specific biomolecules to achieve controllable drug release kinetics. Co-delivery of ions and biomolecules using MBGs can offer synergistic effects for improved therapeutic outcomes.
ACTA BIOMATERIALIA
(2021)
Review
Biochemistry & Molecular Biology
Thembaninkosi G. Gaule, Ramzi A. Ajjan
Summary: Fibrinogen plays a crucial role in blood clotting, with alterations in its structure or incorporation of antifibrinolytic proteins potentially leading to unstable or persistent clots, increasing the risk of bleeding or thrombosis. Understanding fibrinogen function can help in developing effective therapies to modulate clotting and bleeding risk while maintaining the delicate balance between clot formation and lysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Dzhigangir Faizullin, Yuliya Valiullina, Vadim Salnikov, Pavel Zelenikhin, Yuriy Zuev, Olga Ilinskaya
Summary: We experimentally confirmed the possibility of creating composite protein-polysaccharide hydrogels as delivery vehicles for antitumor RNase binase. The gel matrix, based on non-degrading RG and fibrin, does not require cytotoxic chemical cross-linking agents or divalent cations. By encapsulating the therapeutic enzyme binase in liposomes made from uncharged lipids, its prolonged release with preserved activity can be achieved.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Diana Guimaraes, Artur Cavaco-Paulo, Eugenia Nogueira
Summary: Liposomes are effective drug delivery systems that improve therapeutic efficacy, being nontoxic and biodegradable. Key aspects such as design, production techniques, drug loading, and characterization for in vitro and in vivo performance are essential for successful liposomal formulations. Other topics explored include functionalization, targeting strategies, stability, limitations, and current market applications of liposomes in various therapeutic fields.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Review
Environmental Sciences
Sonika Kumari, Varruchi Sharma, Savita Soni, Ajay Sharma, Abhinay Thakur, Satish Kumar, Kuldeep Dhama, Anil Kumar Sharma, Shashi Kant Bhatia
Summary: Layered double hydroxides (LDHs) are a class of materials with unique structures and properties that have a wide range of applications. LDHs can be synthesized in a cost-effective and environmentally friendly manner through aqueous synthetic pathways, and they have the advantages of easy modification and controlled release. LDHs have great potential in drug delivery, as they can stabilize drugs, enhance solubility, and control release. Additionally, LDHs have good biocompatibility and cellular uptake. This study demonstrates the applications of LDHs and their hybrids/composites in drug, cosmeceutical, and gene delivery systems.
ENVIRONMENTAL RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Niranjan G. Kotla, Srinivasa Reddy Bonam, Swetha Rasala, Jitendra Wankar, Raghvendra A. Bohara, Jagadeesh Bayry, Yury Rochev, Abhay Pandit
Summary: Hyaluronan, a naturally occurring biopolymer, has diverse biological effects and plays a crucial role in inflammation and cancer. This review discusses the sources, properties, and potential applications of hyaluronan in therapeutic systems and cancer targeting delivery.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Virology
Junyao He, Linying Yu, Xiaodi Lin, Xiaoyan Liu, Yanming Zhang, Fan Yang, Wen Deng
Summary: Virus-like particles (VLPs) are assembled nanostructures made from viral proteins, and they have been widely used for vaccine development and as nanocarriers for cargo delivery. VLPs have good cell penetrating property, making them ideal for intracellular delivery of biomolecules and drugs. This article summarizes the current advances and applications of VLPs as delivery vectors, including the delivery of different types of biomolecules and drugs, and highlights the strategies for cargo packaging. The production and applications of VLPs are briefly reviewed, along with a discussion on future challenges in this field.
Review
Biochemistry & Molecular Biology
Santhoshkumar Jayakodi, Raghul Senthilnathan, Akila Swaminathan, Venkat Kumar Shanmugam, Rajeshkumar Shanmugam, Anbarasu Krishnan, Vinoth Kumar Ponnusamy, Pei-Chien Tsai, Yuan-Chung Lin, Yi-Hsun Chen
Summary: This review provides a comprehensive overview of the benefits of green synthesis of bioinspired nanoparticles (NPs) using plant extracts and their therapeutic application in cardiovascular diseases (CVDs). NPs can reduce the interaction with biomolecules and prevent the stimulation of reactive oxygen species (ROS), thus reducing the risk of CVD.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Materials Science, Multidisciplinary
Subhasis Roy, Prasenjit Mukherjee, Pradip Kumar Das, Prabal Ranjan Ghosh, Pradyot Datta, Biswanath Kundu, Samit Kumar Nandi
Summary: Musculoskeletal disorders and bone deficiencies are important human health conditions today, with conventional therapy often being ineffective. Local drug delivery systems offer potential advantages in the management of bone disorders.
MATERIALS TODAY COMMUNICATIONS
(2021)
Review
Pharmacology & Pharmacy
Peng Gao, Xin Chang, Dagan Zhang, Yafei Cai, Gen Chen, Hao Wang, Tianfu Wang
Summary: Therapeutic nanoparticles designed to enhance treatment efficacy leverage the unique properties of bio-inspired metal nanoclusters, which have advantages like tunable fluorescent capability and good biocompatibility. These bio-inspired metal nanoclusters can effectively combine fluorescent inorganic moieties with biomolecules to create molecular nanoparticles for targeted therapy and bioimaging.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Materials Science, Multidisciplinary
Hyun Lee, Min-Kyu Lee, Kwang-Hee Cheon, In-Gu Kang, CheonIl Park, Tae-Sik Jang, Ginam Han, Hyoun-Ee Kim, Juha Song, Hyun-Do Jung
Summary: This study proposes a dual-body combination achieved through a densification process for simultaneous release of rhBMP-2 and TCH for efficient bone regeneration. Biomimetic titanium scaffolds with significantly different pore characteristics are successfully fabricated, showing mechanical properties suitable as bone substitutes.
MATERIALS & DESIGN
(2021)
Article
Cell & Tissue Engineering
Kieran Joyce, Zaklina Buljovcic, Goran Rosic, Marietta Kaszkin-Bettag, Abhay Pandit
Summary: This article evaluates the regulatory landscape of tissue-engineered products (TEPs) and identifies the trends in clinical trial activity in the European Union (EU). The study highlights the most active member states, commercial involvement, a shift toward the use of allogeneic cells, and a renewed interest in TEP development.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Neurosciences
Vaibhav Patil, Raghvendra Bohara, Carla Winter, Michelle Kilcoyne, Siobhan McMahon, Abhay Pandit
Summary: This study investigated the glycosylation modulation in a mixed glial culture model and identified considerable differences in glycosylation from acute to chronic phases. Clustering analysis revealed dense clusters for control and LPS treated groups, while the cytokine combination group was scattered into different clusters. Lectin immunostaining confirmed the presence of various glycans in astrocytes and microglia, including GalNAc, GlcNAc, mannose, fucose, and sialic acid-binding residues. Inhibition of sialyltransferase suppressed the upregulation of sialic acid expression, resulting in modulation of mitochondrial functions.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Yazhong Bu, Wenwen Zhang, Sergio Martin-Saldana, Amir M. Alsharabasy, Mark Da Costa, Luyao Feng, Ze Zhang, Xinlan Ge, Chonghui Li, Shichun Lu, Abhay Pandit
Summary: This study develops a series of multifunctional bioadhesives using succinic anhydride-modified hyaluronic acid (HASA) and plant-derived tannic acid (TA) with centrifuging and freeze-drying. By varying the ratio of HASA to TA, HASATA 1:2, 1:7, and 1:14 are fabricated with tunable adhesive strength. The bioadhesives accelerate wound healing, exhibit antioxidant properties, and have antibacterial activity, making them suitable for noninvasive wound closure in the clinical setting.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Multidisciplinary Sciences
Paolo Contessotto, Renza Spelat, Federico Ferro, Vaidas Vysockas, Ausra Krivickiene, Chunsheng Jin, Sandrine Chantepie, Clizia Chinello, Audrys G. Pauza, Camilla Valente, Mindaugas Rackauskas, Alvise Casara, Vilma Zigmantaite, Fulvio Magni, Dulce Papy-Garcia, Niclas G. Karlsson, Egle Ereminiene, Abhay Pandit, Mark Da Costa
Summary: The lack of a reproducible pre-clinical model hinders the study of the pathophysiology and possible interventions for non-ST-segment elevation myocardial infarction (NSTEMI). In this study, authors develop an ovine model to reproduce post-ischemic remodeling in NSTEMI and reveal distinct complex sugar moieties in cellular membranes and extracellular matrix patterns in infarcted tissue.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Buesra Guenay, Elizabeth Matthews, Jack Morgan, Marianna A. Tryfonidou, Radka Saldova, Abhay Pandit
Summary: This study characterizes the N-glycome of the nucleus pulposus (NP) in young and mature intervertebral discs, and identifies patterns related to aging. Findings show that the levels of outer arm fucosylation and α(2,3)-linked sialylation change during aging. The glycosylation changes related to aging are different from those involved in the degeneration of the NP.
Review
Nanoscience & Nanotechnology
Inigo Perez-Estenaga, Merari Tumin Chevalier, Estefania Pena, Gloria Abizanda, Amir M. Alsharabasy, Eduardo Larequi, Myriam Cilla, Marta M. Perez, Jon Gurtubay, Manuel Garcia-Yebenes Castro, Felipe Prosper, Abhay Pandit, Beatriz Pelacho
Summary: Ischemic heart disease is a major cause of death worldwide. A collagen hydrogel loaded with pro-angiogenic SDF1 was combined with a compact collagen membrane to create a bilayer collagen-on-collagen scaffold (bCS), capable of efficiently delivering therapeutic growth factors after myocardial infarction (MI). In vitro and in vivo studies demonstrated that the bCS had suitable physicochemical properties, leading to sustained release of SDF1, integration into the heart tissue, anti-inflammatory and pro-angiogenic macrophage presence, revascularization, and improved cardiac function. This multimodal scaffold can serve as a promising matrix for drug delivery and functional recovery after MI.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Biotechnology & Applied Microbiology
Aert F. Scheper, Jack Schofield, Raghvendra Bohara, Thomas Ritter, Abhay Pandit
Summary: Glycosylation is a process of modifying proteins and lipids with complex carbohydrates called glycans. It is not template-driven like genetic transcription or protein translation, but rather dynamically regulated by metabolic flux. This review provides an overview of the metabolic pathways involved in glycan synthesis and explains the dysregulation of glycosylation in inflammation. The resulting inflammatory hyperglycosylation acts as a glycosignature of disease, and studies on metabolic inhibitors targeting critical enzymes have identified potential glycotherapeutic approaches to inflammation.
BIOTECHNOLOGY ADVANCES
(2023)
Review
Pharmacology & Pharmacy
Vaibhav Patil, Raghvendra Bohara, Vijaya Krishna Kanala, Siobhan Mcmahon, Abhay Pandit
Summary: Spinal cord injury leads to chronic inflammation and glial scar formation caused by the activation of microglia and astrocytes. Current anti-inflammatory strategies have limitations and do not consider the progression of glial cell-derived inflammation. Understanding this progression is crucial for evaluating therapeutic strategies. New models can be used as high-throughput screening platforms for novel therapeutics.
DRUG DISCOVERY TODAY
(2023)
Article
Materials Science, Biomaterials
Mangesh Morey, Aitor Larranaga, Sunny Akogwu Abbah, Raghvendra Bohara, Amal Aljaabary, Abhay Pandit
Summary: Nucleic acid therapy has the potential to promote wound healing through gene expression modification, but protecting the nucleic acid payload and achieving efficient bioresponsive delivery remain challenging. In this study, a glucose-responsive gene delivery system was designed using fibrin-coated polymeric microcapsules, which effectively loaded and released multiple nucleic acids with no cytotoxic effects in vitro. When applied to diabetic wounds, the system improved wound healing and upregulated key proteins involved in the process. This hydrogel-based system shows promise for promoting wound healing and can be encapsulated with different therapeutic nucleic acids.
Article
Cell & Tissue Engineering
Clara Sanz-Nogues, Michael Creane, Sean O. Hynes, Xizhe Chen, Christine Ayu Lagonda, Katarzyna Goljanek-Whysall, Timothy O'Brien
Summary: The aim of this study was to develop and validate a multiparametric scoring tool for assessing the severity of skeletal muscle ischaemia in a commonly used preclinical animal model. The study found a significant negative correlation between the level of muscle ischaemia damage and the calf muscle weight and skeletal muscle fibre diameter.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Review
Medicine, General & Internal
Wang-Lin Duan, Li-Ning Zhang, Raghvendra Bohara, Sergio Martin-Saldana, Fei Yang, Yi-Yang Zhao, Yong Xie, Ya-Zhong Bu, Abhay Pandit
Summary: This review summarizes the advances in using adhesive hydrogel for osteoarthritis (OA) treatment, including commonly used hydrogels with their adhesion mechanisms and components, as well as bioactive therapeutic strategies. By presenting an interdisciplinary perspective, this review aims to provide a comprehensive insight for designing novel bioadhesive systems for OA therapy.
MILITARY MEDICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Enrico Bagnoli, Alexandre Trotier, Jill Mcmahon, Leo R. Quinlan, Manus Biggs, Abhay Pandit, Una Fitzgerald
Summary: Parkinson's disease is a progressive neurodegenerative disorder with unknown etiology and no cure. The olfactory bulb, responsible for processing olfactory information, is affected early in the disease. Research has shown that exposure to the dopamine metabolite DOPAL can replicate many aspects of Parkinson's disease pathology.
Article
Biochemistry & Molecular Biology
Enrico Bagnoli, Alexandre Trotier, Jill McMahon, Leo R. Quinlan, Manus Biggs, Abhay Pandit, Una Fitzgerald
Summary: Parkinson's disease is a progressive neurodegenerative disorder with unknown etiology and no cure. The olfactory bulb, involved in processing olfactory information, is affected early in the disease. The study demonstrates that dopamine metabolite DOPAL can replicate Parkinson's pathology, shedding light on the disease mechanism.
Review
Medicine, Research & Experimental
Catherine R. Healy, Georgina Gethin, Abhay Pandit, David P. Finn
Summary: Chronic wounds impose a significant burden on individuals and the healthcare system, with pain being the most challenging aspect of living with these wounds. The cutaneous endocannabinoid system shows potential in addressing wound-related pain and inflammation, as well as promoting wound healing. However, more research, including preclinical studies and randomized control trials, is needed to fully explore the therapeutic applications of the endocannabinoid system in chronic wound management.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Meeting Abstract
Pharmacology & Pharmacy
Claire Riordan, Lindsay Deely, Sarah Gundy, Brendan Dolan, Abhay Pandit, John P. Kelly, David P. Finn, Eilis Dowd
BRITISH JOURNAL OF PHARMACOLOGY
(2023)