Review
Medicine, General & Internal
Robert Ossendorff, Alessandra Menon, Frank A. Schildberg, Pietro S. Randelli, Sebastian Scheidt, Christof Burger, Dieter C. Wirtz, Davide Cucchi
Summary: The global distribution and quality of studies using adipose tissue derivates in orthopedics were assessed through a systematic review. Among the 82 included studies, 70% focused on knee disorders, mainly osteoarthritis. Approximately 26% of the studies investigated expanded adipose-derived stem/stromal cells (ADSCs), with 72% of these using stromal vascular fraction (SVF). The overall quality of the studies varied, with a higher score found in studies involving expanded ADSCs compared to SVF.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Gabriele Scattini, Martina Pellegrini, Giulio Severi, Monica Cagiola, Luisa Pascucci
Summary: Adipose-derived MSCs are commonly isolated by collagenase digestion, but waste tissue fragments contain cells that adhere belatedly to the plastic. In this study, three sets of cells that adhered at different time points were analyzed for their properties, and no significant differences were found between the three subpopulations. This procedure improves MSCs yield and shortens the time required for clinical applications.
Article
Cell & Tissue Engineering
Roman Vuerich, Elena Groppa, Simone Vodret, Nadja Annelies Ruth Ring, Chiara Stocco, Fleur Bossi, Chiara Agostinis, Matteo Cauteruccio, Andrea Colliva, Mohammad Ramadan, Francesca Simoncello, Federica Benvenuti, Anna Agnelli, Franca Dore, Flavia Mazzarol, Massimo Moretti, Alice Paulitti, Silvia Palmisano, Nicolo De Manzini, Mattia Chiesa, Manuel Casaburo, Angela Raucci, Daniela Lorizio, Giulio Pompilio, Roberta Bulla, Giovanni Papa, Serena Zacchigna
Summary: Nonhealing wounds have a negative impact on the quality of life for patients and health systems. Skin substitutes have been used, but have limited efficacy due to inadequate vascularization. The use of the stromal vascular fraction (SVF) from adipose tissue shows promise in overcoming this limitation, but additional research is needed to determine its precise mechanism of action.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Cell & Tissue Engineering
Fermin Robledo, Lila Gonzalez-Hodar, Pablo Tapia, Ana-Maria Figueroa, Fernando Ezquer, Victor Cortes
Summary: Researchers successfully extracted multipotent cells from the brown adipose tissue of newborn mice, forming adipose spheroids with complex organoid structure and enhanced leptin secretion upon insulin stimulation. These findings have important implications for the potential therapeutic application of adipose tissue-derived stromal vascular fraction cells.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Biochemistry & Molecular Biology
Ibrahim Vargel, Ali Tuncel, Nilsu Baysal, Irem Hartuc-Cevik, Feza Korkusuz
Summary: SVF has the potential to modulate inflammation and initiate regeneration in joint tissues, making it a potential approach for treating knee osteoarthritis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Lucija Zenic, Denis Polancec, Damir Hudetz, Zeljko Jelec, Eduard Rod, Dinko Vidovic, Mario Staresinic, Srecko Sabalic, Trpimir Vrdoljak, Tadija Petrovic, Fabijan Cukelj, Vilim Molnar, Martin Cemerin, Vid Matisic, Petar Brlek, Zrinka Djukic Koroljevic, Igor Boric, Gordan Lauc, Dragan Primorac
Summary: The therapy using mesenchymal stem/stromal cells (MSC) shows promise in treating knee osteoarthritis, but lacks standardized protocols. By comparing stromal vascular fractions from adipose tissue, differences in MSC marker expression and progenitor cell ratios between female and male patients were observed, indicating a differential phenotype pattern even after microfragmentation. This study provides insights into the heterogeneity of adipose MSC subpopulations for osteoarthritis treatment, emphasizing interesting differences between women and men.
Article
Biochemistry & Molecular Biology
Stefan Wolf, Jeremy W. Deuel, Maija Hollmen, Gunther Felmerer, Bong-Sung Kim, Mauro Vasella, Lisanne Gruenherz, Pietro Giovanoli, Nicole Lindenblatt, Epameinondas Gousopoulos
Summary: Lipedema is characterized by disproportionate adipose tissue increase in the limbs, with a distinct systemic cytokine profile and metabolic activity compared to healthy adipose tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Surgery
Kevin M. Urlaub, Kavitha Ranganathan, Jeremy V. Lynn, Alexandra O. Luby, Lauren N. Patrick, Noah S. Nelson, Alexis Donneys, Steven R. Buchman
Summary: Stromal vascular fraction therapy has shown significant efficacy in enhancing bone healing in irradiated fracture repair, improving union rates and bone vascularization metrics while restoring bone cellularity and maturity despite preoperative irradiation. This study provides a promising foundation for the development of noncultured, cell-based therapies for clinical implementation.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2021)
Review
Oncology
Rawan Al-kharboosh, Jonathan Jude Perera, Alexandra Bechtle, Guojun Bu, Alfredo Quinones-Hinojosa
Summary: Neurodegenerative disorders are characterized by the irreversible loss of cognitive functions and CNS structures, and the lack of preventive and regenerative measures has led to traditional symptomatic management as the only available therapeutic recourse. However, recent research suggests that the adipose-derived stromal vascular fraction (SVF) may hold promise as a cellular treatment for neurodegenerative diseases, with improvements seen in clinical applications for conditions such as Multiple Sclerosis, Alzheimer's disease, and Parkinson's disease.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Paul Girard, Joelle Dulong, Jerome Duisit, Camille Mocquard, Simon Le Gallou, Benoit Chaput, Elise Lupon, Eric Watier, Audrey Varin, Karin Tarte, Nicolas Bertheuil
Summary: This study optimized the nanofat grafting technique to increase the yield of adipose-derived stem cells (ASCs) extraction. The results showed that the stromal vascular fraction (SVF) obtained by 20 inter-syringe passages contained more cells, including ASCs, and had better anti-inflammatory properties. This study provides an important foundation for decellularized tissue methods that can provide the required quantity and quality of cells in a clinical setting.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Veterinary Sciences
Khan Sharun, Kaveri Jambagi, Rohit Kumar, Mudasir Bashir Gugjoo, Abhijit M. Pawde, Hardeep Singh Tuli, Kuldeep Dhama, Amarpal
Summary: Adipose tissue-derived stromal vascular fraction (AdSVF) is a heterogeneous cell population with therapeutic potential for various conditions, including osteoarthritis, wound healing, and orthopaedic disorders. Compared to in vitro expanded adipose tissue-derived mesenchymal stem cells (AdMSCs), AdSVF has several advantages.
VETERINARY QUARTERLY
(2022)
Review
Cell Biology
S. Gandolfi, B. Pileyre, L. Drouot, I. Dubus, I. Auquit-Auckbur, J. Martinet
Summary: Muscle regeneration is a complex process that requires precise interaction between immune and muscular cells. The mechanisms underlying autoimmune myopathies are not fully understood, but chronic inflammation and decreased muscle regenerative capacity play a role. Immunosuppressive treatments can be ineffective, and alternative therapies such as stromal vascular fraction show promise but lack a clear understanding of their active components and mechanisms of action.
CELL DEATH DISCOVERY
(2023)
Article
Pharmacology & Pharmacy
Bong-Sung Kim, Shih-Heng Chen, Mauro Vasella, Marco Guidi, Epameinondas Gousopoulos, Nicole Lindenblatt, Huang-Kai Kao
Summary: This study investigates the in vivo fate of mechanically processed stromal vascular fraction (mSVF) for regenerative purposes. By establishing an arteriovenous (AV) shunt model in rats, the study shows that vascularized mSVF can proliferate and express adipocyte-specific markers.
Article
Medicine, Research & Experimental
Linda Vriend, Joris A. van Dongen, Anouk Pijpe, Marianne K. Nieuwenhuis, Sandra J. M. Jongen, Martin C. Harmsen, Paul P. M. van Zuijlen, Berend van der Lei
Summary: This study aims to investigate the effect of tSVF-enriched fat grafting on scar pliability over a 12-month period, as well as probing into its clinical efficacy in treating adherent scars.
Review
Pharmacology & Pharmacy
V. M. H. Srinivasa, B. M. Kumar, Prakash Patil, Nikhil Shetty, A. V. Shetty
Summary: This article presents a systematic review on the use of extracellular vesicles (EVs) as a novel therapy for antibiotic-resistant infections. It emphasizes the superiority of adipose tissue as a source of antimicrobial EVs (aEVs) and discusses the limited literature and research gaps in this field.
CURRENT DRUG TARGETS
(2023)
Article
Cardiac & Cardiovascular Systems
Johanna Gueckel, Christian Puelacher, Noemi M. Glarner, Danielle Gualandro, Ivo Strebel, Tobias Zimmermann, Ketina Arslani, Reka Hidvegi, Marcel Liffert, Alessandro Genini, Stella Marbot, Maria Schlaepfer, Luzius Steiner, Daniel Bolliger, Andreas Lampart, Lorenz Guerke, Christoph Kindler, Stefan Scharen, Stefan Osswald, Martin Clauss, Daniel Rikli, Giovanna Lurati Buse, Christian Mueller
Summary: This study evaluated the contributions of patient and procedure-related factors to perioperative myocardial infarction/injury (PMI) in noncardiac surgery. The results showed that procedure-related factors were more strongly associated with PMI occurrence, but patient factors also played a role in PMI development.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2022)
Article
Biochemical Research Methods
Gordian Born, Evelia Plantier, Guido Nannini, Alessandro Caimi, Andrea Mazzoleni, M. Adelaide Asnaghi, Manuele G. Muraro, Arnaud Scherberich, Ivan Martin, Andres Garcia-Garcia
Summary: Bioreactors that allow direct perfusion of cell suspensions or culture media through 3D scaffolds are widely used in tissue engineering to improve cell seeding efficiency, uniformity of cell distribution, and tissue development. A macro-scale U-shaped bioreactor has been previously developed, but the perfusion flow in the peripheral regions was not uniform. In this study, the design of the perfusion chamber has been optimized to provide more homogeneous perfusion flow through the scaffold. Furthermore, a miniaturized version of the flow-optimized bioreactor has been proposed for medium-throughput assays.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Clinical Neurology
Brian A. Karamian, Gregory D. Schroeder, Mark J. Lambrechts, Jose A. Canseco, Cumhur Oner, Emiliano Vialle, Shanmuganathan Rajasekaran, Marcel R. Dvorak, Lorin M. Benneker, Frank Kandziora, Klaus Schnake, Christopher K. Kepler, Alexander R. Vaccaro
Summary: The purpose of this study was to validate the AO Spine Subaxial Injury Classification System, and the results showed that the system had excellent interobserver reliability and intraobserver reproducibility for fracture morphology, substantial reliability and reproducibility for facet injuries, and excellent reproducibility with substantial reliability for injury subtype. In conclusion, this classification system is accurate and reliable in assessing and describing subaxial cervical spine injuries.
EUROPEAN SPINE JOURNAL
(2023)
Article
Engineering, Biomedical
Yawei Gu, Sebastien Pigeot, Lucas Ahrens, Fabian Tribukait-Riemenschneider, Melika Sarem, Francine Wolf, Andres Garcia-Garcia, Andrea Barbero, Ivan Martin, V. Prasad Shastri
Summary: By developing a bioink system with appropriate rheological properties and using 3D bioprinting technology, it is possible to create high cell density structures of predefined geometry, which can then be implanted into nude mice to generate bone tissue with neovascularization. This method demonstrates the potential for fabricating complex bone tissue for clinical applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Cell Biology
Boris Dasen, Sebastien Pigeot, Gordian Manfred Born, Sophie Verrier, Olga Rivero, Petra S. Dittrich, Ivan Martin, Maria Filippova
Summary: This study found that pericytes express not only the classical N-cadherin, but also the atypical T-cadherin. T-cadherin plays an important role in regulating the proliferation, migration, invasion, and interactions of pericytes with endothelial cells.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell & Tissue Engineering
Sowmya Viswanathan, Katarina Le Blanc, Rachele Ciccocioppo, Georges Dagher, Anthony J. Filiano, Jacques Galipeau, Mauro Krampera, Lena Krieger, Manoj M. Lalu, Jan Nolta, Viviana Marcela Rodriguez Pardo, Yufang Shi, Karin Tarte, Daniel J. Weiss, Ivan Martin
Summary: The International Standards Organization (ISO) has recently published ISO standardization documents for MSC(WJ) and MSC(M) biobanking, which standardize the terminology and functional characterization of MSCs. These documents, developed with input from ISCT, provide requirements and recommendations for functional characterization of MSC(WJ) and MSC(M) and represent an international consensus on MSC identity and characterization. They are an important first step in standardizing MSC biobanking and characterization for research and development.
Article
Cell & Tissue Engineering
Anke Wixmerten, Sylvie Miot, Patrick Bittorf, Francine Wolf, Sandra Feliciano, Stephan Hackenberg, Sebastian Hasner, Werner Krenger, Martin Haug, Ivan Martin, Oliver Pullig, Andrea Barbero
Summary: This article describes the regulatory compliant change of a raw material in the development of a medicinal product. The substitution of autologous serum with clinical-grade human platelet lysate (hPL) in the manufacturing process was shown to enhance cell proliferation and gene expression without significant changes in product quality. The modified process was accepted by national competent authorities and applied in ongoing clinical tests of N-TEC.
Article
Cell Biology
Encheng Ji, Lieke Leijsten, Janneke Witte-Bouma, Adelin Rouchon, Nunzia Di Maggio, Andrea Banfi, Gerjo J. V. M. van Osch, Eric Farrell, Andrea Lolli
Summary: Tissue engineering bone via endochondral ossification requires the generation of a mineralized cartilage template. Our study investigated the effects of cartilage mineralization on its pro-angiogenic potential. We found that mineralized cartilage showed decreased production of pro-angiogenic factors and reduced ability to stimulate endothelial cell migration, proliferation, and tube formation. This stage-dependent pro-angiogenic potential should be considered in bone tissue engineering strategies.
Article
Biotechnology & Applied Microbiology
Jesil Kasamkattil, Anna Gryadunova, Raphael Schmid, Max Hans Peter Gay-Dujak, Boris Dasen, Morgane Hilpert, Karoliina Pelttari, Ivan Martin, Stefan Scharen, Andrea Barbero, Olga Krupkova, Arne Mehrkens
Summary: An advanced 3D microtissues model containing human degenerating nucleus pulposus tissue was developed to mimic the microenvironment of early-stage degenerative disc disease. Nasal chondrocytes in spheroidal organization showed superior regenerative performance compared to cell suspension, and pre-conditioning with IL-1Ra further improved their ability to counteract inflammation and support new matrix production. This study is of great importance for therapeutic strategies in degenerative disc disease.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Andres Garcia-Garcia, Sebastien Pigeot, Ivan Martin
Summary: The contribution of the immune system to tissue regeneration is increasingly recognized, suggesting that tissue engineering strategies and biomaterials design should focus on regulating early immunological processes. This study aimed to enhance the osteoinductivity of engineered extracellular matrices (ECMs) by enriching their repertoire of immunomodulatory factors. The results showed that priming with IL1 beta and overexpressing BMP-2 enabled the engineering of ECMs with preserved osteoinductive signals and increased levels of angiogenic and pro-inflammatory molecules. These IL1 beta-induced ECMs enhanced the inflammatory phase and ultimately led to faster and more efficient bone formation.
BIOACTIVE MATERIALS
(2023)
Article
Cell & Tissue Engineering
Andrea Grosso, Alexander Lunger, Maximilian G. Burger, Priscilla S. Briquez, Francesca Mai, Jeffrey A. Hubbell, Dirk J. Schaefer, Andrea Banfi, Nunzia Di Maggio
Summary: Vascular endothelial growth factor-A (VEGF) has a physiological role in regulating both angiogenesis and osteogenesis, but its application in bone tissue engineering has produced conflicting outcomes. This study investigates the impact of VEGF dose on the coordination between angiogenesis and osteogenesis. The findings suggest that a specific range of VEGF dose is required to stimulate both vascular invasion and osteogenic commitment, resulting in improved bone formation. The study also reveals that VEGF dose regulates Notch1 activation and induces a pro-osteogenic endothelial phenotype. Fine-tuning of VEGF dose is crucial for achieving the physiological coupling of accelerated vascular invasion and enhanced bone formation.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Orthopedics
M. Kamenova, E. Li, J. Soleman, O. Fiebig, A. Mehrkens, S. Schaeren
Summary: The aim of this study was to compare reoperation rates and clinical outcomes between TLIF and posterior stabilization with PEEK rods in elderly patients with one-level lumbar spine degenerative disease. The results showed similar reoperation rates and outcomes between the TLIF and PEEK groups, but the TLIF group had higher intraoperative blood loss, administration of tranexamic acid, and operation time. In patients above 80 years of age, the TLIF group had higher reoperation rates as well.
ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY
(2023)
Meeting Abstract
Cell & Tissue Engineering
Manuele Giuseppe Muraro, Andrea Mazzoleni, Evrim Ceren Kabak, Arnaud Scherberich, Ivan Martin
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Mansoor Chaaban, Adrien Moya, Thibaut Klein, Arnaud Scherberich
TISSUE ENGINEERING PART A
(2022)