Article
Biology
Diptiman Chanda, Mohammad Rehan, Samuel R. Smith, Kevin G. Dsouza, Yong Wang, Karen Bernard, Deepali Kurundkar, Vinayak Memula, Kyoko Kojima, James A. Mobley, Gloria A. Benavides, Victor Darley-Usmar, Young-iL Kim, Jaroslaw W. Zmijewski, Jessy S. Deshane, Stijn De Langhe, Victor J. Thannickal
Summary: This study revealed the impact of cellular aging on the ability of L-MSC and AEC2s cells to form three-dimensional organoids, and found that reducing Nox4 activity may reverse critical effects of cellular aging.
Article
Polymer Science
Bryan Falcones, Hector Sanz-Fraile, Esther Marhuenda, Irene Mendizabal, Ignacio Cabrera-Aguilera, Nanthilde Malandain, Juan J. Uriarte, Isaac Almendros, Daniel Navajas, Daniel J. Weiss, Ramon Farre, Jorge Otero
Summary: Mesenchymal stromal cell (MSC) therapy for acute respiratory diseases relies on MSCs' secretion of paracrine factors. Preconditioning the MSCs by growing them in a lung-derived extracellular matrix (ECM) can enhance their therapeutic efficacy. Bioink based on decellularized porcine lung ECM hydrogels supports 3D culturing of lung-resident MSCs without additional crosslinking, leading to improved cell-matrix interactions and increased expression of key receptors. This approach shows promise for enhancing MSC preconditioning for therapeutic applications.
Article
Biotechnology & Applied Microbiology
Jie Chen, Shiyang Ma, Baihua Luo, Haojie Hao, Yanqin Li, Hang Yang, Fei Zhu, Peipei Zhang, Ruichao Niu, Pinhua Pan
Summary: In this study, it was found that small extracellular vesicles (sEVs) derived from mesenchymal stromal cells (MSC sEVs) could transfer microRNAs (miRNAs) to attenuate inflammation in lung epithelial cells during acute lung injury (ALI). Animal experiments and in vitro cell experiments confirmed this mechanism. MiR-223-3p was identified as a critical mediator in the regulatory effects induced by MSC sEVs. These findings suggest that MSC sEVs may offer a novel promising strategy for ALI.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Materials Science, Biomaterials
Jiayi Zhu, Wanqing Lun, Qi Feng, Xiaodong Cao, Qingtao Li
Summary: The mechanical stiffness of the ECM affects YAP activity, which in turn regulates the differentiation of stem cells in cartilage tissue development and regeneration. However, the mechanisms underlying scaffold-associated mechanobiology during chondrogenesis initiation and hyaline cartilage maintenance are not well understood. In this study, we investigated the relationship between YAP subcellular localization and changes in cartilage structure during early postnatal growth. We also studied the effect of scaffold stiffness on hyaline cartilage formation by mesenchymal stem cells and the role of YAP activity.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Medicine, General & Internal
Tiago Ramos, Mohit Parekh, Paula Meleady, Finbarr O'Sullivan, Rosalind M. K. Stewart, Stephen B. Kaye, Kevin Hamill, Sajjad Ahmad
Summary: The ocular surface is composed of two different types of epithelial cells, corneal and conjunctival epithelium. The migration of conjunctival epithelium onto the corneal surface can result in incomplete transdifferentiation and changes in cell fate determination. The extracellular matrix (ECM) proteins play a role in inducing cell differentiation and modulating the expression of specific markers. The response of epithelial cells to ECM proteins is laminin-type dependent, and the levels of phosphorylation and proteolysis of proteins involved in cell growth and differentiation are influenced by ECM proteins.
FRONTIERS IN MEDICINE
(2022)
Article
Cell & Tissue Engineering
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: The study identifies HSPB7 as a regulator of osteogenic and adipogenic differentiation in bone marrow-derived mesenchymal stromal cells (BMSCs), with a positive role in osteoblastic differentiation and maintaining the balance between osteogenesis and adipogenesis. HSPB7 shows potential as a therapeutic target for bone metabolic diseases.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Tim Tian Y. Han, John T. Walker, Aaron Grant, Gregory A. Dekaban, Lauren E. Flynn
Summary: The study shows that bioreactor preconditioning enhances the regenerative potential of human ASCs by altering the expression characteristics of ASCs on DAT scaffolds through in vitro culture, and also promotes the infiltration of host cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Immunology
Alasdair G. Kay, James M. Fox, James P. Hewitson, Andrew P. Stone, Sophie Robertson, Sally James, Xiao-nong Wang, Elizabeth Kapasa, Xuebin B. Yang, Paul G. Genever
Summary: The heterogeneity of bone marrow mesenchymal stromal cells (MSCs) poses challenges for their clinical development. A subtype of MSCs characterized by CD317 expression has been identified, and they show a constitutive interferon signature linked to human disease. CD317(pos) MSCs induce tissue damage in a skin inflammation model, while CD317(neg) MSCs have no effect. CD317(neg) MSCs can suppress the proliferation of activated human T cells, while CD317(pos) MSCs increase the polarization towards pro-inflammatory Th1 cells. CD317(neg) and CD317(pos) MSCs can suppress leukocyte recruitment, but only CD317(neg) MSCs suppress macrophage numbers.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Mengmeng Fan, Makoto Arai, Akinobu Tawada, Tetsuhiro Chiba, Reo Fukushima, Katsuhiro Uzawa, Masashi Shiiba, Naoya Kato, Hideki Tanzawa, Yuichi Takiguchi
Summary: The study revealed the roles of EPSTI1 in conferring malignant characteristics to oral squamous cell carcinoma (OSCC) and lung squamous cell carcinoma (LSCC), with the gene showing oncogenic roles in OSCC and tumor-suppressive roles in LSCC. Additionally, PCR array analyses identified some genes with altered expression levels along with modified EPSTI1 expression in both OSCC and LSCC cell lines, suggesting EPSTI1 may be a therapeutic target for both types of cancer.
Article
Biochemistry & Molecular Biology
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: In this study, TNS3 was found to be involved in the fate decision of human bone marrow-derived stromal cells (BMSCs), regulating osteogenesis and adipogenesis through the control of RhoA activity and cytoskeletal reorganization. The findings highlight the crucial role of TNS3 in bone metabolism.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Immunology
Takeshi Nitta, Hiroshi Takayanagi
Summary: The stromal microenvironment in the thymus is crucial for generating a functional T cell repertoire, with thymic epithelial cells (TECs) being the most prominent type. Besides TECs, other stromal cell types of mesenchymal origin also play important roles in controlling TEC development. The recently discovered functional effect of thymic fibroblasts on T cell repertoire selection is highlighted as a significant advancement in our understanding of thymic organogenesis and T cell development.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Zinat Sargazi, Saeed Zavareh, Mojdeh Salehnia
Summary: This study aimed to construct an endometrial-like structure using co-culturing of human mesenchymal endometrial cells and uterine smooth muscle cells on a decellularized scaffold. Different cell seeding methods were compared, and the one with the highest number of seeded cells and lowest number of residual cells in suspension was selected for further study. The co-cultured cells showed differentiation and expressed endometrial-related genes, indicating the successful formation of endometrial-like structures.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2023)
Article
Cell & Tissue Engineering
A. S. Croft, J. Fuhrer, M. Woltje, B. Gantenbein
Summary: Researchers have successfully created silk scaffolds with similar size and cell density to human intervertebral disc tissue using silk and human mesenchymal stromal cells. These scaffolds have shown good biocompatibility in terms of cell numbers and morphology, and have promoted tissue-like characteristics.
EUROPEAN CELLS & MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Huda I. Atiya, Grace Gorecki, Geyon L. Garcia, Leonard G. Frisbie, Roja Baruwal, Lan Coffman
Summary: The ability of cancer cells to detach from the primary site and metastasize is the main cause of cancer-related death. The first event in the metastatic cascade is epithelial-to-mesenchymal transition (EMT), which leads to the loss of cell-cell adhesion and the acquisition of motile and stem-like phenotypes. The tumor microenvironment (TME) plays a critical role in modulating EMT in cancer cells, promoting the acquisition of a mesenchymal phenotype through interaction or changes with cancer cells.
Article
Engineering, Biomedical
Saik-Kia Goh, Suzanne Bertera, Thomas Richardson, Ipsita Banerjee
Summary: Diabetes is a global epidemic affecting more than 285 million people worldwide. Engineering endocrine pancreas tissue using regenerative cell source shows promise for diabetes therapy. Researchers demonstrate the feasibility of re-engineering decellularized organ scaffolds using human pluripotent stem cells (hPSC) to repopulate these scaffolds. The study finds that hPSCs cultured and differentiated as aggregates are more suitable for repopulation, especially in decellularized liver scaffolds, which also support the endocrine cell fate of the engrafted cells. This novel strategy has potential applications in preclinical testing, drug discovery, and diabetes therapy.
BIOMEDICAL MATERIALS
(2023)