Article
Engineering, Biomedical
Jia-Pu Liang, Robert P. Accolla, Madhuvanthi Soundirarajan, Amy Emerson, Maria M. Coronel, Cherie L. Stabler
Summary: The use of OxySite microbeads incorporated within a macroporous PDMS scaffold allowed for sufficient local oxygenation for up to 20 days without toxic by-products. This approach showed promising potential for clinically relevant cell transplantation, highlighting the superior translational potential of off-the-shelf OxySite scaffolds for improving graft outcomes.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Ioannis Eugenis, Di Wu, Caroline Hu, Gladys Chiang, Ngan F. Huang, Thomas A. Rando
Summary: Volumetric muscle loss (VML) is an irreversible loss of skeletal muscle due to trauma or surgery. In this study, a macroporous dECM-methacrylate (dECM-MA) hydrogel was developed and found to promote cellularization, endothelialization, and establishment of a pro-regenerative immune microenvironment. The hydrogel also enhanced the proliferation of transplanted primary muscle stem cells, muscle tissue regeneration, and functional recovery.
Article
Chemistry, Applied
Hideaki Tokuyama, Ryo Aoyagi, Kazuto Fujita, Yuki Maekawa, Shohei Riya
Summary: Ethanol fermentation using a hybrid system containing free and immobilized yeast cells in macroporous hydrogel scaffold was studied, with immobilization achieved through NIPA and MTGA hydrogels. The hybrid system showed higher ethanol productivity than the control system, with macroporous hydrogel allowing stable growth of immobilized yeast cells. In steady-state fermentation, the hybrid system had significantly higher cell densities and ethanol productivities compared to the control system.
REACTIVE & FUNCTIONAL POLYMERS
(2021)
Article
Materials Science, Biomaterials
Elizabeth Bealer, Kelly Crumley, Daniel Clough, Jessica King, Maya Behrend, Connor Annulis, Feiran Li, Scott Soleimanpour, Lonnie D. Shea
Summary: Studies have shown that three-dimensional cell culture of human stem cell-derived islet organoids can improve cell differentiation and function. Transplantation of these organoids into extrahepatic sites reduces hyperglycemia.
BIOMATERIALS SCIENCE
(2023)
Article
Cell & Tissue Engineering
Jia-Pu Liang, Robert P. Accolla, Kaiyuan Jiang, Ying Li, Cherie L. Stabler
Summary: This study demonstrates the potential of using a microbead scaffold platform for the local delivery of anti-inflammatory and proangiogenic agents, which can control the release of multiple drugs and promote the healthy integration of implants with host tissues. This multifunctional platform could be beneficial for various cellular transplants and engineered tissues.
TISSUE ENGINEERING PART A
(2021)
Article
Engineering, Biomedical
Meadhbha Brennan, David S. Monahan, Benedicte Brulin, Sara Gallinetti, Paul Humbert, Christina Tringides, Cristina Canal, Maria Pau Ginebra, Pierre Layrolle
Summary: In this study, the healing capacity of CDHA administering MSCs to bone defects was evaluated for the first time and compared with sintered beta-TCP constructs. Greater cell infiltration occurred in CDHA scaffolds, and after 8 weeks in vivo, MSC engraftment was higher in CDHA compared to beta-TCP, as was bone in-growth.
ACTA BIOMATERIALIA
(2021)
Article
Surgery
Salamah M. Alwahsh, Omar Qutachi, Philip J. Starkey Lewis, Andrew Bond, June Noble, Paul Burgoyne, Nik Morton, Rod Carter, Janet Mann, Sofia Ferreira-Gonzalez, Marta Alvarez-Paino, Stuart J. Forbes, Kevin M. Shakesheff, Shareen Forbes
Summary: Liver-targeted FGF7-GAL-PLGA particles were shown to enhance islet engraftment and improve blood glucose control in diabetic mice, with good safety profile. This targeted approach could be a promising strategy to optimize islet transplantation outcomes in type 1 diabetes.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Polymer Science
Prabha Govindaraj, Dhamodharan Raghavachari
Summary: A novel method for synthesizing a macroporous chitosan hydrogel scaffold with partial physical and chemical crosslinking was introduced in this study. The scaffolds showed promising biocompatibility and potential for applications in hemostasis and drug delivery, although the current system has limitations in fabricating microporous chitosan with higher porosity and surface area.
JOURNAL OF POLYMER RESEARCH
(2021)
Article
Endocrinology & Metabolism
Darling Rojas-Canales, Stacey N. Walters, Daniella Penko, Daniele Cultrone, Jacqueline Bailey, Tatyana Chtanova, Jodie Nitschke, Julie Johnston, Svjetlana Kireta, Thomas Loudovaris, Thomas W. Kay, Tim R. Kuchel, Wayne Hawthorne, Philip J. O'Connell, Greg Korbutt, John E. Greenwood, Shane T. Grey, Chris J. Drogemuller, P. Toby Coates
Summary: A clinically proven biodegradable temporizing matrix (BTM) has been found to facilitate the formation of a well-vascularized intracutaneous neodermis within the skin, which improves the survival and functionality of transplanted islet cells. The experiments conducted on mice and pigs showed that BTM does not harm the viability and function of islet cells, and it can maintain the normal structure and function of the islets. BTM has the potential to become an alternative site for islet transplantation.
Review
Engineering, Biomedical
Robert P. Accolla, Amberlyn M. Simmons, Cherie L. Stabler
Summary: With the increasing prevalence of endocrine diseases like type 1 diabetes mellitus (T1DM), there is a growing need for cellular replacement approaches. Additive manufacturing (AM) techniques offer improved control over construct geometry and microscale component placement, addressing the limitations of traditional fabrication methods. This review discusses the potential of AM approaches in T1DM cellular transplantation and their broader application in cell-based therapy.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Cell Biology
Erika Sugisawa, Takeshi Kondo, Yutaro Kumagai, Hiroki Kato, Yasunori Takayama, Kayako Isohashi, Eku Shimosegawa, Naoki Takemura, Yoshinori Hayashi, Takuya Sasaki, Mikael M. Martino, Makoto Tominaga, Kenta Maruyama
Summary: This study identified a nociceptor-derived hormone called Reg3y, which can protect the host from endotoxic death by modulating the kynurenine pathway in the brain.
Review
Endocrinology & Metabolism
Smit N. Patel, Clayton E. Mathews, Rachel Chandler, Cherie L. Stabler
Summary: Progress in diabetes research is hindered by current deficiencies in experimental systems. Engineering human-centric platforms that closely mimic human physiology requires careful design. Understanding the features of the pancreatic islet and the limitations of existing models is important for improving diabetes research. Emerging platforms that combine diabetes-relevant cells in dynamic environments show promise but need further development. Engineers can use a proposed roadmap to create biorelevant and accessible diabetes models, while biologists are encouraged to utilize these tools for complex questions. The ultimate goal is to translate research from the lab to the clinic.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Engineering, Biomedical
Ruyi Wang, Shijie Cao, Mohamed Elfatih H. Bashir, Lauren A. Hesser, Yanlin Su, Sung Min Choi Hong, Andrew Thompson, Elliot Culleen, Matthew Sabados, Nicholas P. Dylla, Evelyn Campbell, Riyue Bao, Eric B. Nonnecke, Charles L. Bevins, D. Scott Wilson, Jeffrey A. Hubbell, Cathryn R. Nagler
Summary: The depletion of beneficial taxa in the gut, including butyrate-producing Clostridia, has led to the increase in food allergies and inflammatory bowel disease. By delivering butyrate-releasing micelles to the intestinal tract, barrier-protective responses can be restored, reducing disease severity in mouse models of colitis and peanut allergy. This approach can potentially treat allergic and inflammatory diseases by restoring microbial and mucosal homeostasis.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Endocrinology & Metabolism
Marco Infante, Camillo Ricordi
Summary: Diabetes of the exocrine pancreas (DEP) refers to different types of diabetes resulting from disorders of the exocrine pancreas. Post-total pancreatectomy diabetes mellitus (PTPDM) is a unique form of DEP with distinct pathophysiological and clinical features.
EXPERT REVIEW OF ENDOCRINOLOGY & METABOLISM
(2023)
Editorial Material
Endocrinology & Metabolism
Teresa L. Mastracci, Minoti Apte, Laufey T. Amundadottir, Alexandra Alvarsson, Steven Artandi, Melena D. Bellin, Ernesto Bernal-Mizrachi, Alejandro Caicedo, Martha Campbell-Thompson, Zobeida Cruz-Monserrate, Abdelfattah El Ouaamari, Kyle J. Gaulton, Andrea Geisz, Mark O. Goodarzi, Manami Hara, Rebecca L. Hull-Meichle, Alexander Kleger, Alison P. Klein, Janel L. Kopp, Rohit N. Kulkarni, Mandar D. Muzumdar, Anjaparavanda P. Naren, Scott A. Oakes, Soren S. Olesen, Edward A. Phelps, Alvin C. Powers, Cherie L. Stabler, Temel Tirkes, David C. Whitcomb, Dhiraj Yadav, Jing Yong, Norann A. Zaghloul, Stephen J. Pandol, Maike Sander
Summary: The workshop was held to promote research on pancreatic diseases and provide guidance for future directions. It covered various aspects including pancreas anatomy and physiology, diabetes in the setting of exocrine disease, metabolic influences on the exocrine pancreas, genetic drivers of pancreatic diseases, tools for integrated pancreatic analysis, and implications of exocrine-endocrine crosstalk. The discussions identified research gaps and opportunities for better understanding the interplay between these compartments through integration of current knowledge.
Article
Engineering, Biomedical
Robert P. Accolla, Jia-Pu Liang, Taylor R. Lansberry, Irayme Labrada Miravet, Marlon Loaisiga, Baltasar Lopez Sardi, Cherie L. Stabler
Summary: An oxygen-generating composite material called OxySite is developed by encapsulating calcium peroxide (CaO2) within polydimethylsiloxane and formulating it into microbeads for tissue integration. Material parameters are modulated to study oxygen generation kinetics and cellular applications. In vitro and in vivo experiments demonstrate the improved metabolic activity and function of cells when coencapsulated with optimized OxySite microbeads.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Cell & Tissue Engineering
Andrea Grosso, Alexander Lunger, Maximilian G. Burger, Priscilla S. Briquez, Francesca Mai, Jeffrey A. Hubbell, Dirk J. Schaefer, Andrea Banfi, Nunzia Di Maggio
Summary: Vascular endothelial growth factor-A (VEGF) has a physiological role in regulating both angiogenesis and osteogenesis, but its application in bone tissue engineering has produced conflicting outcomes. This study investigates the impact of VEGF dose on the coordination between angiogenesis and osteogenesis. The findings suggest that a specific range of VEGF dose is required to stimulate both vascular invasion and osteogenic commitment, resulting in improved bone formation. The study also reveals that VEGF dose regulates Notch1 activation and induces a pro-osteogenic endothelial phenotype. Fine-tuning of VEGF dose is crucial for achieving the physiological coupling of accelerated vascular invasion and enhanced bone formation.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Cell & Tissue Engineering
Yasmin K. Alshoubaki, Yen-Zhen Lu, Julien M. D. Legrand, Rezvan Karami, Mathilde Fossat, Ekaterina Salimova, Ziad Julier, Mikael M. Martino
Summary: Cytokines and growth factors have potential in regenerative medicine, but their clinical success has been limited due to safety concerns and low effectiveness. By exploiting interactions between extracellular matrix (ECM) and therapeutic proteins, researchers have developed an engineering strategy that significantly enhances the tissue retention of therapeutic proteins and reduces leakage in circulation. This approach shows promise in improving the efficacy and safety of regenerative therapies.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Engineering, Biomedical
Robert P. Accolla, Madison Deller, Taylor R. Lansberry, Amberlyn Simmons, Jia-Pu Liang, Smit N. Patel, Kaiyuan Jiang, Cherie L. Stabler
Summary: This study addresses the challenge of compacted, dense, and poorly functional microcapillary structures in pre-vascularization approaches by introducing a 3D printed PDMS-based open framework structure. The PDMS framework reduces hydrogel compaction, resulting in easily manipulated constructs with increased surface area. Additionally, the PDMS frame alters vascular morphogenesis, leading to larger and less dense network structures. This research provides a novel approach to improve the generation of translational pre-vascularized networks.
ACTA BIOMATERIALIA
(2023)
Article
Immunology
Marco Infante, Laura Vitiello, Andrea Fabbri, Camillo Ricordi, Nathalia Padilla, Francesca Pacifici, Pasquale Di Perna, Marina Passeri, David Della-Morte, Massimiliano Caprio, Luigi Uccioli
Summary: We describe a case of a 22-year-old Italian man with new-onset type 1 diabetes who experienced an unusually prolonged clinical remission phase (31 months). The patient was treated with calcifediol and low-dose insulin, resulting in the maintenance of residual beta-cell function and clinical remission. This may be attributed to the immunoregulatory properties of calcifediol.
Article
Engineering, Biomedical
Abigail L. Lauterbach, Anna J. Slezak, Ruyi Wang, Shijie Cao, Michal M. Raczy, Elyse A. Watkins, Carlos J. Medina Jimenez, Jeffrey A. Hubbell
Summary: Butyrate, an important bacterial metabolite, has a complex role in regulating immunity and maintaining epithelial barriers. However, its clinical translation is hindered by poor bioavailability, strong odor, and high dosage requirements. A novel polymeric delivery platform has been developed for controlled release of butyrate, which also has potential for other therapeutically relevant metabolites. This platform shows efficacy in the treatment of non-healing diabetic wounds by modulating immune cell activation and altering the soluble signals in the wound microenvironment, addressing a significant clinical need.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Gastroenterology & Hepatology
Teresa L. Mastracci, Minoti Apte, Laufey T. Amundadottir, Alexandra Alvarsson, Steven Artandi, Melena D. Bellin, Ernesto Bernal-Mizrachi, Alejandro Caicedo, Martha Campbell-Thompson, Zobeida Cruz-Monserrate, Abdelfattah El Ouaamari, Kyle J. Gaulton, Andrea Geisz, Mark O. Goodarzi, Manami Hara, Rebecca L. Hull-Meichle, Alexander Kleger, Alison P. Klein, Janel L. Kopp, Rohit N. Kulkarni, Mandar D. Muzumdar, Anjaparavanda P. Naren, Scott A. Oakes, Soren S. Olesen, Edward A. Phelps, Alvin C. Powers, Cherie L. Stabler, Temel Tirkes, David C. Whitcomb, Dhiraj Yadav, Jing Yong, Norann A. Zaghloul, Maike Sander, Stephen J. Pandol
Summary: The workshop focused on the integration of knowledge about the physiology of the exocrine and endocrine compartments in pancreatic diseases. It aimed to establish connections and identify research gaps and opportunities in the field. The presentations and discussions covered various topics, including pancreas anatomy and physiology, diabetes in the setting of exocrine disease, metabolic influences on the exocrine pancreas, genetic drivers of pancreatic diseases, tools for integrated pancreatic analysis, and implications of exocrine-endocrine crosstalk. The discussions resulted in a better understanding of the interplay between these compartments and highlighted the importance of integrating current knowledge for future research.
Meeting Abstract
Cell & Tissue Engineering
Adelin Rouchon, Priscilla S. Briquez, Dirk J. Schaefer, Jeffrey A. Hubbell, Nunzia Di Maggio, Andrea Banfi
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Andrea Banfi, Andrea Grosso, Priscilla S. Briquez, Dirk J. Schaefer, Jeffrey A. Hubbell, Nunzia Di Maggio
TISSUE ENGINEERING PART A
(2022)