期刊
TISSUE ENGINEERING PART A
卷 18, 期 3-4, 页码 397-410出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/ten.tea.2010.0414
关键词
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资金
- National Science Foundation [CBET 0966546]
- Institute for Critical Technologies and Applied Science at Virginia Tech
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1067654] Funding Source: National Science Foundation
Bone regeneration can be accelerated by utilizing mechanical stress and growth factors (GFs). However, a limited understanding exists regarding the response of preosteoblasts to tensile stress alone or with GFs. We measured cell proliferation and expression of heat-shock proteins (HSPs) and other bone-related proteins by preosteoblasts following cyclic tensile stress (1%-10% magnitude) alone or in combination with bone morphogenetic protein-2 (BMP-2) and transforming growth factor-beta 1 (TGF-beta 1). Tensile stress (3%) with GFs induced greater gene upregulation of osteoprotegerin (3.3 relative fold induction [ RFI] compared to sham-treated samples), prostaglandin E synthase 2 (2.1 RFI), and vascular endothelial growth factor (VEGF) (11.5 RFI), compared with samples treated with stimuli alone or sham-treated samples. The most significant increases in messenger RNA expression occurred with GF addition to either static-cultured or tensile-loaded (1% elongation) cells for the following genes: HSP47 (RFI = 2.53), cyclooxygenase-2 (RFI = 72.52), bone sialoprotein (RFI = 11.56), and TGF-beta 1 (RFI = 8.05). Following 5% strain with GFs, VEGF secretion increased 64% (days 3-6) compared with GF alone and cell proliferation increased 23% compared with the sham-treated group. GF addition increased osteocalcin secretion but decreased matrix metalloproteinase-9 significantly (days 3-6). Tensile stress and GFs in combination may enhance bone regeneration by initiating angiogenic and anti-osteoclastic effects and promote cell growth.
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