Article
Biochemistry & Molecular Biology
Lukasz Oldak, Sylwia Chludzinska-Kasperuk, Patrycja Milewska, Kamil Grubczak, Joanna Reszec, Ewa Gorodkiewicz
Summary: This study investigated the use of LN-5, FN, and COL IV as potential biomarkers for brain glioma. The results showed that the concentration changes of these proteins were related to the severity of the disease and had a moderate correlation with patient age. The findings suggest that these proteins may have potential clinical applications in non-invasive diagnosis and determining the grade of brain glioma.
Article
Cell Biology
Amanda C. Daulagala, Antonis Kourtidis
Summary: The extracellular matrix (ECM) plays a crucial role in tissue homeostasis, and abnormalities in ECM composition can lead to pathological conditions. In this study, the researchers investigated the influence of different ECM substrates on the localization of RNA interference (RNAi) complexes in colon epithelial cells. They found that fibronectin and collagens I and IV disrupted the junctional localization of RNAi components and their interactions with essential partners. These findings suggest a potential mechanism that could contribute to epithelial cell transformation.
Article
Chemistry, Analytical
Tomasz Guszcz, Anna Sankiewicz, Ewa Gorodkiewicz
Summary: This study aimed to determine the blood serum concentration of collagen IV, laminin-5 and fibronectin in bladder cancer patients and correlate them with clinicopathological parameters. The results showed that the serum concentrations of these biomarkers were associated with the diagnosis and staging of bladder cancer.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Cell Biology
Claire A. Gianakas, Daniel P. Keeley, William Ramos-Lewis, Kieop Park, Ranjay Jayadev, Isabel W. Kenny, Qiuyi Chi, David R. Sherwood
Summary: The study identifies the crucial role of hemicentin and fibulin-1 in initiating and strengthening basement membrane (BM) attachment. The mechanisms underlying BM-BM linkage maturation are revealed, providing new insights into this specialized form of tissue linkage.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Chemistry, Organic
Qi Zhou, Rongbin Cai, Huan Li, Yunzhi Liu, Bing Li, Tianjiao Hou, Long Zhu, Guixiang Wang, Yu Zhang, Jun Luo
Summary: A two-shell arrangement strategy was successfully employed for the synthesis of nitro-substituted azaadamantane compounds. The target product, along with its derivative, exhibited excellent detonation properties, including high density, detonation velocity, and pressure. Moreover, this strategy provides a new route for the synthesis of polynitroazaadamantane compounds with more than six nitro groups.
ORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Pharmacology & Pharmacy
Jun Iwashita, Hikari Maeda, Momo Ishimura, Jun Murata
Summary: The excess secretion of MUC5AC in the respiratory tract of asthma patients can be suppressed by type IV collagen exposure, which modulates integrin expression and Akt/ERK phosphorylation in the respiratory tract of OVA-sensitized mice.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Toxicology
Somaieh Mansouri, Mehdi Jalali, Mohammad Reza Nikravesh, Mohammad Soukhtanloo
Summary: The study found that METH administration led to decreased expression levels of collagen type IV and laminin alpha 5 in the testis of adult mice. These changes may negatively impact fertility by altering the extracellular matrix content.
Article
Clinical Neurology
Apoorva A. Kumar, Natalie Yeo, Max Whittaker, Priya Attra, Thomas R. Barrick, Leslie R. Bridges, Dennis W. Dickson, Margaret M. Esiri, Chad W. Farris, Delyth Graham, Wen Lang Lin, Daniel N. Meijles, Anthony C. Pereira, Gregory Perry, Douglas L. Rosene, Anan B. Shtaya, Tom Van Agtmael, Giovanna Zamboni, Atticus H. Hainsworth
Summary: Vascular collagen-IV in cerebral small arteries of older people is negatively associated with radiological SVD severity, but not related to history of hypertension. Reduced collagen-IV is accompanied by accumulation of fibrillar collagens. Brain collagen-IV is elevated in older normotensive primates, but not associated with hypertension. Collagen-IV in arterial tissue does not differ between genetically hypertensive and normotensive rats.
Article
Medicine, General & Internal
Yukun Bai, Ping Wang, Yanwei Qi, Yang Li, Weidong Liu, Lei Gao, Hanliang Jiao, Yanxiao An, Yingxin Gong
Summary: The diagnostic value of HA, PC-III, IV-C, and LN in infants with congenital biliary atresia (CBA) was investigated in this study. The levels of these indicators were significantly increased in the CBA group compared to the noncongenital biliary atresia group. HA, PC-III, IV-C, and LN showed high accuracy for the diagnosis of CBA in infants and could be potential diagnostic biomarkers for CBA.
Article
Engineering, Biomedical
Lens M. Dedroog, Olivier Deschaume, Christian J. Garcia Abrego, Erin Koos, Yovan de Coene, Anja Vananroye, Wim Thielemans, Carmen Bartic, Minne P. Lettinga
Summary: This study reports a cell-compatible method based on shear stress, which allows for the fabrication of 3D uniformly aligned fibrillar collagen hydrogels. The method offers reproducibility and tunability, with minimal impact on the viability of embedded cells.
ACTA BIOMATERIALIA
(2022)
Article
Materials Science, Multidisciplinary
Zeying Zhang, Gui-Bin Liu, Zhi-Ming Yu, Shengyuan A. Yang, Yugui Yao
Summary: The research focuses on emergent particles stabilized by the symmetries of type-IV magnetic space groups (MSGs), which have a special time reversal symmetry {T vertical bar t(0)}. A complete classification of these particles in type-IV MSGs is provided, along with easily accessible interactive tables containing their basic information. The study reveals the occurrence of emergent particles in spinful systems that were previously believed to exist only in spinless systems due to the {T vertical bar t(0)} symmetry.
Article
Ophthalmology
Annalisa Altera, Gian Marco Tosi, Mari Regoli, Elena De Benedetto, Eugenio Bertelli
Summary: The study analyzed the composition of internal limiting membrane (ILM) of the retina and idiopathic epiretinal membranes (iERMs) using immunofluorescence and confocal microscopy, finding differences in thickness and protein expression between the two membranes. The ILM contained laminins, collagens, and entactin, while iERMs expressed a subset of these proteins. The co-localization of major constituents in iERMs suggested a deranged process of basement membrane formation.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2021)
Article
Chemistry, Organic
Mathieu Morency, Sebastien Neron, Radu Iftimie, James D. Wuest
Summary: This study established a simple method based on a linear relationship to predict pK(a) values of quinonoid compounds, with high accuracy for handling complex analogues with multiple OH groups and intramolecular hydrogen bonds. The approach allows reliable predictions of acidity, selection of proper pK(a) values, and determination of deprotonation sites and orders in complex structures.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Chemistry, Physical
V. Abinaya, D. John Thiruvadigal, R. Akash, A. Sakthi Balaji, R. M. Hariharan, J. Sneha, U. Adharsh, K. Janani Sivasankar
Summary: In this study, the structural and electronic properties of aluminum nitride nanotubes (AlNNT) chemically modified with various biologically available functional groups were investigated using density functional theory. It was found that the band gap of the AlNNT can be reduced by chemical functionalization, making it a potential candidate for drug delivery applications.
SURFACES AND INTERFACES
(2023)
Article
Medicine, General & Internal
Jasmina Comic, Korbinian M. Riedhammer, Roman Guenthner, Christian W. Schaaf, Patrick Richthammer, Hannes Simmendinger, Donald Kieffer, Riccardo Berutti, Velibor Tasic, Nora Abazi-Emini, Valbona Nushi-Stavileci, Jovana Putnik, Natasa Stajic, Adrian Lungu, Oliver Gross, Lutz Renders, Uwe Heemann, Matthias C. Braunisch, Thomas Meitinger, Julia Hoefele
Summary: Disease-causing variants in COL4A3-5 are associated with type IV-collagen-related nephropathy, a genetically and phenotypically diverse disorder. Early diagnosis is crucial for optimal therapy. This study highlights the complex clinical and genetic manifestations of individuals with this condition, emphasizing the need for a refined nomenclature and interdisciplinary collaboration.
FRONTIERS IN MEDICINE
(2022)
Review
Biochemical Research Methods
Udesh Dhawan, Hussain Jaffery, Manuel Salmeron-Sanchez, Matthew J. Dalby
Summary: This review discusses recent breakthroughs in understanding the complex interplay between cellular nanoenvironment and biomolecular signalling pathways, and the development of targeted osteogenic platforms. It also presents strategies and techniques for inducing osteogenesis, as well as overcoming the drawbacks of traditional osteogenic implants.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Cell Biology
Andrew Y. Wang, Nuno M. Coelho, Pamma D. Arora, Yongqiang Wang, Denise Eymael, Chenfan Ji, Qin Wang, Wilson Lee, Jessica Xu, Andras Kapus, Karina M. M. Carneiro, Christopher A. McCulloch
Summary: Fibrosis is characterized by excessive deposition of densely packed collagen fibrils, which hampers organ structure and function. A key player in collagen-rich lesions is the discoidin domain receptor 1 (DDR1), which is associated with increased collagen compaction through Ca2+-dependent nonmuscle myosin IIA (NMIIA). This study reveals a functional relationship between DDR1 and the transient receptor potential vanilloid type 4 (TRPV4) channel, showing that DDR1 regulates Ca2+ influx through TRPV4 to promote critical processes involved in collagen compaction and alignment in fibrotic lesions.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Giuseppe Ciccone, Mariana Azevedo Gonzalez Oliva, Nelda Antonovaite, Ines Luchtefeld, Manuel Salmeron-Sanchez, Massimo Vassalli
Summary: Nanoindentation is an experimental technique used to quantify the local mechanical properties of soft biomaterials and cells. The most popular approach, using an atomic force microscope (AFM), is complex and limits routine measurements. Recently, nanoindentation devices based on optical fiber sensing technology have gained popularity for their ease of integration and ability to apply sub-nN forces with mu m spatial resolution.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Review
Engineering, Biomedical
Jiajun Luo, Matthew Walker, Yinbo Xiao, Hannah Donnelly, Matthew J. Dalby, Manuel Salmeron-Sanchez
Summary: This review explores the effective physical approach of nanotopography in biomaterial cell manipulation and its interactions with protein adsorption. Advances in creating nanotopographical features to guide cell behaviors and the cellular mechanotransductive pathways initiated by nanotopography are summarized in this review.
BIOACTIVE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Michaela Petaroudi, Aleixandre Rodrigo-Navarro, Oana Dobre, Matthew J. Dalby, Manuel Salmeron-Sanchez
Summary: Living interfaces are a novel class of active materials that allow precise control over cell behavior. This study presents a biointerface between engineered bacteria and mammalian cells, which successfully regulates the stemness markers of human mesenchymal stem cells.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Jiajun Luo, Shudong Zhao, Xiangsheng Gao, Swastina Nath Varma, Wei Xu, Maryam Tamaddon, Richard Thorogate, Haoran Yu, Xin Lu, Manuel Salmeron-Sanchez, Chaozong Liu
Summary: This study investigated the mechanism of cellular adhesion on nanotopographic features, revealing that cells exhibit better adhesion and spreading on nanoconvex surfaces with a stronger and more concentrated evolution of Coulomb's force. This research is significant for designing novel Ti-based orthopaedic implants' surfaces.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Biomedical
Yinbo Xiao, ChanelleA. S. McGuinness, W. Sebastian Doherty-Boyd, Manuel Salmeron-Sanchez, Hannah Donnelly, Matthew J. Dalby
Summary: Hematopoietic stem cells play a fundamental role in the generation of blood and immune cells. Although the signaling mechanisms in their bone marrow niche microenvironment have been increasingly understood, the ability to recreate these mechanisms in vitro for clinical purposes is still lacking. Recent studies have used engineering techniques to establish ex vivo bone marrow niche models, showing promising potential for research and clinical applications.
Article
Engineering, Biomedical
Michaela Petaroudi, Aleixandre Rodrigo-Navarro, Oana Dobre, Matthew J. Dalby, Manuel Salmeron-Sanchez
Summary: Living biointerfaces are a new type of biomaterials that combine living cells and polymeric matrices to act as instructive materials for surrounding cells. In this study, living biomaterials based on Lactococcus lactis expressing specific genes were used to control hematopoietic stem cells in both 2D and 3D environments. The results showed that these living biomaterials can direct the interaction between stem cells and bacteria, and can significantly expand the population of specific stem cells in 3D hydrogels. The findings suggest the potential of using genetically engineered bacteria-based living materials for ex-vivo expansion of stem cells and clinical applications in hematological disorders.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Cell Biology
Ezequiel Monferrer, Oana Dobre, Sara Trujillo, Mariana Azevedo Gonzalez Oliva, Alexandre Trubert-Paneli, Delia Acevedo-Leon, Rosa Noguera, Manuel Salmeron-Sanchez
Summary: The tumor microenvironment plays a crucial role in cancer development, and the use of decoupled elements in 3D in vitro systems is important for studying cancer progression. In this study, hydrogels incorporating VN into PEG networks were developed to mimic the native NB microenvironment. Results showed that VN-cell interaction plays a key role in NB aggressiveness.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Vasileios Papalazarou, James Drew, Amelie Juin, Heather J. Spence, Jamie Whitelaw, Colin Nixon, Manuel Salmeron-Sanchez, Laura M. Machesky
Summary: Pancreatic cancer is a deadly and highly metastatic disease. This study explores the influence of stiff extracellular matrix (ECM) on pancreatic cancer cell behavior and suggests that pancreatic cancer cells can be reprogrammed when exposed to a soft microenvironment. Collagen VI plays a key role in this process.
JOURNAL OF CELL SCIENCE
(2022)
Article
Engineering, Biomedical
Yinbo Xiao, Hannah Donnelly, Mark Sprott, Jiajun Luo, Vineetha Jayawarna, Leandro Lemgruber, P. Monica Tsimbouri, R. M. Dominic Meek, Manuel Salmeron-Sanchez, Matthew J. Dalby
Summary: In the field of bone tissue regenerative medicine, the use of MSC-based tissue engineering strategies combined with growth factors (GFs) and biomaterials has drawn great interest. However, the optimization of interactions between biomaterials and MSCs, as well as the efficient delivery of GFs, remains a challenge. This study demonstrates that through plasma polymerization, tissue culture plates coated with poly (ethyl acrylate) (PEA) can provide an optimal environment for MSCs, with enhanced adhesion and binding of GFs. The combination of fibronectin (FN) and vitronectin (VN) on PEA surfaces results in the formation of a heterogeneous, open ECM network, promoting MSC differentiation into osteoblasts and inducing osteogenic intracellular signaling.
MATERIALS TODAY BIO
(2022)
Review
Engineering, Biomedical
Alvaro Sanchez-Rubio, Vineetha Jayawarna, Emily Maxwell, Matthew J. Dalby, Manuel Salmeron-Sanchez
Summary: Tissue engineering aims to replicate tissues and organs for in vivo and in vitro applications. In vivo, artificial constructs using functional materials and cells are engineered to provide physiological form and function. In vitro, three-dimensional (3D) models are engineered to support drug discovery and enable understanding of fundamental biology. Recent advances in fabrication technologies have enabled compartmentalized structures with defined compositions and properties, which are crucial in creating 3D cell-laden multiphasic complex architectures. This review focuses on the advances in engineered multicompartment constructs that mimic tissue heterogeneity, highlighting the relevance of 3D bioprinting in the future of biological research and medicine.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Stylianos O. Sarrigiannidis, Oana Dobre, Alexandre Rodrigo Navarro, Matthew J. Dalby, Cristina Gonzalez-Garcia, Manuel Salmeron-Sanchez
Summary: Researchers have developed a recombinant dual affinity protein fragment that can immobilize bone morphogenic protein 2 (BMP-2) in collagen sponges, addressing the low affinity issue between BMP-2 and collagen. This technology enables osteogenesis with ultra-low doses of BMP-2 and enhances the biological activity of collagen, providing a potential pathway for clinical translation without complex chemistries or manufacturing changes.
MATERIALS TODAY BIO
(2023)
Article
Nanoscience & Nanotechnology
Miranda Morata-Martinez, Mark R. Sprott, Carmen M. Antolinos-Turpin, Manuel Salmeron-Sanchez, Gloria Gallego-Ferrer
Summary: Fibronectin (FN) plays a significant role in cell-material interactions for tissue repair. The spontaneous organization of FN in poly(ethyl acrylate) (PEA) and poly(buthyl acrylate) (PBA) substrates leads to exceptional bone regeneration and efficient cell differentiation. By synthesizing P(EA-co-BA) copolymers, researchers investigate the impact of intermediate surface mobility on cell differentiation. The results show that as the PBA content increases, FN mobility increases, cell adhesion strengthens, and cell differentiation decreases.
ACS APPLIED BIO MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Hannah Donnelly, Mark R. Sprott, Anup Poudel, Paul Campsie, Peter Childs, Stuart Reid, Manuel Salmeron-Sanchez, Manus Biggs, Matthew J. Dalby
Summary: There is a clinical demand for viable bone grafts, and tissue engineering strategies using mesenchymal stromal cells (MSCs) to create potential bone-graft materials can help fulfill this need. However, the long culture periods and cost associated with MSC osteogenesis in a clinical setting necessitate the investigation of strategies for optimizing cell production. In this study, a piezoelectric copolymer with a fibronectin network coating is used to enhance MSC adhesion and present growth factors, and electrical stimulation is applied to investigate the MSC response.
ACS APPLIED MATERIALS & INTERFACES
(2023)