Review
Engineering, Biomedical
Kenichi Arai, Takahiro Kitsuka, Koichi Nakayama
Summary: The safety and efficacy of new drugs are typically tested in animals, but differences in drug responses and potential cardiac side effects have led to many drug development projects being discontinued. Alternative methods to animal testing are needed. Tissue engineering techniques can create three-dimensional cardiac constructs from hiPSC-CMs, providing a new approach for drug screening.
Review
Cell Biology
Venkata Suresh Venkataiah, Yoshio Yahata, Akira Kitagawa, Masahiko Inagaki, Yusuke Kakiuchi, Masato Nakano, Shigeto Suzuki, Keisuke Handa, Masahiro Saito
Summary: Bone tissue engineering involves combining MSCs with a biocompatible scaffold to aid in bone regeneration and recovery. While promising results have been seen in clinical trials, regenerating large bone defects remains a challenge.
Article
Nanoscience & Nanotechnology
Libo Zhou, Wuxia Zhang, Jinhyung Lee, Liisa Kuhn, Yupeng Chen
Summary: This study developed an injectable nanomatrix (NM) scaffold with a layer-by-layer structure inside each nanosized fiber for highly localized drug delivery and a homeostatic microenvironment, successfully generating highly organized structures via molecular self-assembly.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Yurui Xu, Anwei Zhou, Weiwei Chen, Xinghai Ning
Summary: This study presents a novel bio-orthogonal engineering strategy (BIEN) to create advanced multicellular 3D tissue constructs (MTCs) using a scaffold-free approach. By harnessing the cellular biosynthetic machinery, BIEN incorporates bio-orthogonal azide reporters into cell surface glycoconjugates and assembles MTCs through a click reaction with multiarm PEG. The resulting MTCs demonstrate uniform, heterogeneous spheroids with high intercellular junction and pluripotency, simulating tumor features and improving research models.
Article
Biophysics
Lin Tang, Xiaoying Chen, Mei Wang, Yuhua Liu, Bowen Li, Yuke Li, Yi Zhang
Summary: This study explores the construction of an organic-inorganic composite scaffold for bone regeneration, using decellularized small intestinal submucosa (SIS) as the organic component and phosphorylated hydroxyapatite nanocrystals (nHA) as the inorganic component. The nHA@SIS scaffold showed superior physicochemical properties and osteogenic ability compared to the SIS scaffold, indicating great potential for bone tissue engineering.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Polymer Science
Mahmoud Amr, Alia Mallah, Samina Yasmeen, Bernard Van Wie, Arda Gozen, Juana Mendenhall, Nehal I. Abu-Lail
Summary: This study examines the efficacy of a biocompatible hydrogel-based scaffold (SA-GEL-GA) in supporting the proliferation, extracellular matrix (ECM) production, and pericellular matrix (PCM) production of bovine articular chondrocytes (bAChs). The results demonstrate that SA-GEL-GA is not cytotoxic, promotes cellular proliferation, and supports ECM and PCM deposition by bAChs.
Article
Nanoscience & Nanotechnology
Pengfei Cai, Chunchun Li, Yangfan Ding, Hanting Lu, Xiao Yu, Jie Cui, Fan Yu, Hongsheng Wang, Jinglei Wu, Mohamed EL-Newehy, Meera Moydeen Abdulhameed, Liang Song, Xiumei Mo, Binbin Sun
Summary: This research introduces a novel method that combines electrospun nanofibers with 3D printing techniques to fabricate composite scaffolds for bone defect treatment. Compared to nanoparticle-reinforced scaffolds, these scaffolds infused with nanofibers exhibit superior mechanical attributes and bioactivity.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Igor Rzhepakovsky, Sergey Piskov, Svetlana Avanesyan, Marina Sizonenko, Lyudmila Timchenko, Oxana Anfinogenova, Andrey Nagdalian, Andrey Blinov, Evgeniya Denisova, Stanislav Kochergin, Sergey Kubanov, Magomed Shakhbanov, Mohammad Ali Shariati, Mohammad S. Mubarak
Summary: Pure and porous bacterial cellulose scaffolds were synthesized using Medusomyces gisevii Sa-28 bacterial strain. Modification with gelatin increased the biocompatibility and solubility of the scaffolds. The scaffolds exhibited uniform distribution of pores, promoting new blood vessel growth and collagen fiber formation. The results suggest that these scaffolds can be used for engineering hard and soft tissues.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Francesca Pescosolido, Luca Montaina, Rocco Carcione, Sara Politi, Roberto Matassa, Felicia Carotenuto, Stefania Annarita Nottola, Paolo Di Nardo, Emanuela Tamburri
Summary: Conductive hybrid xanthan gum (XG)-polyaniline (PANI) biocomposites with 3D structures were synthesized in a strong-acid free medium. The composites showed electrical conductivity and electrochemical activity, and exhibited biocompatibility in trial tests on prostate cancer cells. This research opens up new possibilities for the use of these materials in biomedical applications.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Chemistry, Applied
Pariya Zare, Mohamad Pezeshki-Modaress, Seyed Mohammad Davachi, Pouria Zare, Fatemeh Yazdian, Sara Simorgh, Hadi Ghanbari, Hamid Rashedi, Zohreh Bagher
Summary: In this study, two different arrangements of laminated composite scaffolds were fabricated based on Alginate, showcasing improved mechanical features and sustained drug release. The optimized scaffold displayed advantages such as interconnected pores, controlled biodegradability, and no cytotoxic effect, making it a promising candidate for tissue regeneration.
CARBOHYDRATE POLYMERS
(2021)
Article
Polymer Science
Amaliya Rasyida, Salma Halimah, Ika Dewi Wijayanti, Sigit Tri Wicaksono, Haniffudin Nurdiansah, Yohannes Marudut Tua Silaen, Yatim Lailun Ni'mah, Hosta Ardhyananta, Agung Purniawan
Summary: This study investigated the interrelation between the addition of reduced graphene oxide (r-GO) into PVA/Alginate and their degradation and biocompatibility properties. The morphology of the composites was characterized using scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS), and the mechanical properties were determined through a compressive test. In vitro cytotoxicity and cell viability were assessed using an MTT assay. The presence of r-GO was found to have no significant effect on the morphology of the hydrogel, and the addition of 0.4% r-GO increased cell viability, indicating low toxicity. The studied composites showed minimal degradation and outstanding biocompatibility, making them attractive materials for tissue engineering applications.
Article
Cell & Tissue Engineering
Siddharth Shanbhag, Salwa Suliman, Samih Mohamed-Ahmed, Carina Kampleitner, Mohamed Nageeb Hassan, Patrick Heimel, Toni Dobsak, Stefan Tangl, Anne Isine Bolstad, Kamal Mustafa
Summary: Three-dimensional spheroid culture can promote osteogenic differentiation of bone marrow mesenchymal stromal cells, while 3D printing allows for customized scaffolds for complex bone defects. This study compared the potential of human BMSC cultured as 2D monolayers or 3D spheroids in constructs for bone regeneration. Results showed a trend for superior in vitro mineralization with 3D BMSC, but similar in vivo performance between 2D and 3D BMSC, indicating PLATMC-HPLG constructs as promising scaffolds for bone tissue engineering.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Qingxi Hu, Junjie Wu, Haiguang Zhang, Wenpei Dong, Yan Gu, Suihong Liu
Summary: A degradable hernia repair patch with an anti-adhesive layer was prepared using 3D printing and electrospinning techniques. It showed superior mechanical strength and biocompatibility compared to commercial patches, effectively reducing the occurrence of adhesions.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Polymer Science
Ssu-Meng Haung, Yu-Ting Lin, Shih-Ming Liu, Jian-Chih Chen, Wen-Cheng Chen
Summary: In this study, porous scaffolds made of gelatin, hyaluronic acid, alginate, and sucrose in different proportions were used for chondrocyte regeneration. The results showed that the scaffold with the highest porosity and strength did not perform the best, while another scaffold provided a more suitable environment for chondrocyte growth. Developing hydrogels with customized pore properties for defective cartilage is expected to meet the requirements of ultimate clinical applications.
Article
Biochemistry & Molecular Biology
Fan Fei, Haiyan Yao, Yujiang Wang, Junchao Wei
Summary: Poly(lactide)/graphene oxide/parathyroid hormone (1-34) nanofiber membranes were prepared and showed improved tensile strength and alkaline phosphatase activity, making them promising candidate materials for bone tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Zhicong Zhao, Xia Deng, Jue Jia, Li Zhao, Chenxi Wang, Zhensheng Cai, Chang Guo, Ling Yang, Dong Wang, Suxian Ma, Jialiang Deng, Haoxiang Li, Libin Zhou, Zhigang Tu, Guoyue Yuan
Summary: The study demonstrated that ANGPTL8 improves glucose metabolism in diabetic mice by inhibiting hepatic gluconeogenesis. This suggests that increased expression of ANGPTL8 could be a potential target for preventing and treating type 2 diabetes.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2022)
Review
Nanoscience & Nanotechnology
Ya-Ni Zhang, Zhuo-Ying Song, Dun Qiao, Xiao-Hui Li, Zhe Guang, Shao-Peng Li, Li-Bin Zhou, Xiao-Han Chen
Summary: 2D van der Waals materials, with atomic thickness layers and rich distinct properties, have been extensively used in the field of ultrafast photonics. This article provides a detailed description of the photoelectric characteristics, nonlinear characteristic measurement techniques, and preparation technology of 2D van der Waals materials. It also summarizes the development of ultrafast pulsed fiber lasers based on different types of 2D materials. Furthermore, it reviews and summarizes the opportunities and research results of ultrafast pulsed fiber lasers based on the latest 2D van der Waals materials.
Article
Cell Biology
Shushu Wang, Miaomiao Yuan, Linlin Zhang, Kecheng Zhu, Chunxiang Sheng, Feiye Zhou, Zhaoqian Xu, Qianqian Liu, Yun Liu, Jieli Lu, Xiao Wang, Libin Zhou
Summary: Short-chain fatty acids (SCFAs) produced by the gut microbiota have been shown to improve metabolic homeostasis, but their role in islet function is still controversial. This study found that long-term incubation with sodium acetate, sodium propionate, and sodium butyrate significantly enhanced pancreatic beta cell function, but also suppressed the expression of insulin secretion pathway and beta cell identity genes.
CELL DEATH & DISEASE
(2022)
Article
Pharmacology & Pharmacy
Wan Xu, Feiye Zhou, Qin Zhu, Mengyao Bai, Tiancheng Luo, Libin Zhou, Ruyuan Deng
Summary: The study found that CG can attenuate palmitate-induced lipid accumulation in hepatocytes by activating AMPK, suggesting it may be a promising therapeutic medicine for hepatic steatosis.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Genetics & Heredity
Yuqi Li, Jiayu Gu, Ahsan Irshad, Linshu Zhao, Huijun Guo, Hongchun Xiong, Yongdun Xie, Shirong Zhao, Yuping Ding, Libin Zhou, Fuquan Kong, Zhengwu Fang, Luxiang Liu
Summary: Novel genetic variations can be obtained by inducing mutations in plants. In this study, wheat treated with C-12 and Li-7 ion beam radiation was analyzed, and differentially expressed proteins associated with metabolism and photosynthesis were identified. C-12 and Li-7 ion beam radiation had different effects on plant growth, inhibiting photosynthesis in C-12 treated plants but not in Li-7 treated plants.
FRONTIERS IN GENETICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yudong Liu, Yang Zhou, Lei Qin, Jun Zeng, Haiqiong Yue, Lijuan Zeng, Jianfeng He, Libin Zhou
Summary: The traditional semiburied design of internal distractor has a high risk of infection and aesthetic problems. A modified internal distractor with design of pre-embedding curvilinear rail, drive screw, and universal joint was invented to reduce these risks. Its stress distribution characteristics during curvilinear distraction osteogenesis (DO) were analyzed and its effectiveness was tested in vivo.
JOURNAL OF STOMATOLOGY ORAL AND MAXILLOFACIAL SURGERY
(2023)
Review
Biochemistry & Molecular Biology
Jiaqi Wang, Yixin Zhang, Libin Zhou, Fu Yang, Jingpeng Li, Yan Du, Ruiyuan Liu, Wenjian Li, Lixia Yu
Summary: Seed priming technology is considered a promising strategy for agricultural sciences and food technology to improve crop yield and quality. Compared to conventional methods, physics strategies like ionizing radiation are more effective and environmentally friendly, but further research is needed to explore their effects and mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Jun Zeng, Ye Jiang, Yudong Liu, Yongxin Hu, Chenmiao Guo, Libin Zhou
Summary: The objective of this study was to construct a novel portable tooth sectioning guide to improve the accuracy of mandibular third molar extraction. The results showed that the digital guided technique is more predictable and accurate in tooth sectioning, and it can be successfully performed by dental surgeons with different levels of experience.
JOURNAL OF STOMATOLOGY ORAL AND MAXILLOFACIAL SURGERY
(2023)
Article
Biochemistry & Molecular Biology
Zhuo Feng, Yan Du, Jingmin Chen, Xia Chen, Weibin Ren, Lulu Wang, Libin Zhou
Summary: The study demonstrates that both carbon-ion beam and gamma rays are effective techniques for rapid mutation breeding in soybean. Carbon-ion beams have a higher relative biological effectiveness and can induce a higher proportion of low-frequency phenotypic mutations and structural variants. The optimal dosage range for carbon-ion beam irradiation in soybean is 101 Gy to 115 Gy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Zhaoqian Xu, Yiru Wang, Qianqian Liu, Shushu Wang, Chunxiang Sheng, Junmin Chen, Jialin Tan, Xiao Wang, Li Shao, Libin Zhou
Summary: Excessive hepatic gluconeogenesis plays a role in type 2 diabetes mellitus. Serum- and glucocorticoid inducible-kinase 1 (SGK1) is associated with metabolic syndrome development. The regulatory role of SGK1 in liver glucose metabolism is unclear.
JOURNAL OF MOLECULAR ENDOCRINOLOGY
(2023)
Article
Plant Sciences
Xiao Liu, Yan Du, Xiaofan Na, Man Wang, Ying Qu, Linghui Ge, Yuanmeng Wang, Linqi Gao, Wenke Bai, Yurong Bi, Libin Zhou
Summary: Plant growth-promoting rhizobacteria (PGPR) promote plant growth and protect plants from pathogens, contributing to sustainable agriculture. Integration of transcriptome and metabolome analysis reveals that BT22 inoculation regulates plant hormone homeostasis and induces metabolome reprogramming, thereby promoting growth in Arabidopsis.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Plant Sciences
Weibin Ren, He Wang, Yan Du, Yan Li, Zhuo Feng, Xinhui Zhou, Guisen Kang, Qingyao Shu, Tao Guo, Huijun Guo, Lixia Yu, Wenjie Jin, Fu Yang, Jingpeng Li, Jianzhong Ma, Wenjian Li, Chaoli Xu, Xia Chen, Xiao Liu, Chenan Yang, Luxiang Liu, Libin Zhou
Summary: This study systematically investigated the effects of heavy ion beam (HIB) on rice. It was found that doses exceeding 125 Gy caused significant physiological damages to rice. The mutation rate peaked at 100 Gy, and 129 mutants with distinct phenotypic variations were isolated from 11,720 M-2 plants, with about 50% showing stable inheritance in the next generation. These findings demonstrate the effectiveness of HIB as a breeding tool and provide valuable resources for further genomic research and breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Xia Chen, Yan Du, Shanwei Luo, Ying Qu, Wenjie Jin, Shizhong Liu, Zhuanzi Wang, Xiao Liu, Zhuo Feng, Bi Qin, Libin Zhou
Summary: Taraxacum kok-saghyz Rodin (TKS) shows great potential as an alternative natural-rubber (NR)-producing crop, but its germplasm innovation is hindered by self-incompatibility. This study investigates the potential use of carbon-ion beam (CIB) irradiation for mutation breeding of TKS. Results show that CIB irradiation affects the growth, physiological parameters, and gene expression pattern of TKS. The study provides valuable insights into the radiation response mechanism and future mutation breeding strategies for TKS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Hongchun Xiong, Huijun Guo, Meiyu Fu, Yongdun Xie, Linshu Zhao, Jiayu Gu, Shirong Zhao, Yuping Ding, Qidi Du, Jiazi Zhang, Lin Qiu, Xiaomei Xie, Libin Zhou, Zhongxu Chen, Luxiang Liu
Summary: To overcome the challenge posed by the large genome of hexaploid wheat, researchers generated a wheat mutant database through whole-exome sequencing. The database contains a diverse array of genetic variations induced by different mutagenesis methods, providing a valuable resource for functional genomics studies in wheat.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Astronomy & Astrophysics
Xiao Liu, Yan Du, Chaoli Xu, Fusheng Wang, Xuehu Li, Luxiang Liu, Xiaohui Ma, Yuanmeng Wang, Linghui Ge, Weibin Ren, Ling Jin, Libin Zhou
Summary: This study investigates the molecular response mechanism of Platycodon grandiflorus seedlings after irradiation with heavy ion beams (HIB) and X-rays (XR). Transcriptome and proteome analyses reveal that HIB radiation induces more differentially expressed proteins (DEPs), while XR radiation leads to a higher number of differentially expressed genes (DEGs). Both HIB and XR radiation activate genes associated with RNA silencing, DNA repair, and cellular processes. Additionally, the two types of radiation elicit distinct effects on gene expression and protein enrichment, providing insights into the biological effects of plants under space radiation.
LIFE SCIENCES IN SPACE RESEARCH
(2023)