Article
Biochemistry & Molecular Biology
Emilie J. Doucet, Sergio Cortez Ghio, Martin A. Barbier, Etienne Savard, Brice Magne, Meryem Safoine, Danielle Larouche, Julie Fradette, Lucie Germain
Summary: Tissue-engineered skin substitutes (TESs) used for severe burn injuries have traditionally been cultured using bovine serum, but a new study shows that serum-free media can be used to produce high quality TESs that are macroscopically and histologically similar to those produced with serum. The serum-free TESs expressed the same genes and had comparable mechanical properties to serum-cultured TESs. They were also successfully grafted onto mice for a six-month period.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Nikhil T. Awatade, Andrew T. Reid, Kristy S. Nichol, Kurtis F. Budden, Punnam Chander Veerati, Prabuddha S. Pathinayake, Christopher L. Grainge, Philip M. Hansbro, Peter A. B. Wark
Summary: This study compared the morphology and functional responses of primary bronchial epithelial cells differentiated using two commonly used culture media, PneumaCult and bronchial epithelial growth medium. The results showed distinct structural and functional differences between the two media.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Luis Soriano, Tehreem Khalid, Fergal J. O'Brien, Cian O'Leary, Sally-Ann Cryan
Summary: Translation of novel inhalable therapies for respiratory diseases is hindered by the lack of in vitro cell models reflecting native tissue complexity. Developing physiologically-representative tracheobronchial tissue analogues can improve new treatments translation by accurately reflecting in vivo respiratory responses. The study utilized advanced tissue-engineered collagen hyaluronic acid bilayered scaffolds (CHyA-B) to evaluate bacterial and drug-induced toxicity and inflammation, finding higher inflammatory responses in 3D epithelial cultures compared to 2D.
Article
Biochemistry & Molecular Biology
Sergio Cortez Ghio, Martin A. Barbier, Emilie J. Doucet, Imad Debbah, Meryem Safoine, Gaetan Le-Bel, Andreanne Cartier, Emilie Jolibois, Amelie Morissette, Danielle Larouche, Julie Fradette, Sylvain L. Guerin, Alain Garnier, Lucie Germain
Summary: We developed a new chemically defined serum-free medium, Surge SFM, which can support massive expansion of human primary keratinocytes and production of skin substitutes. Compared to conventional serum-containing medium, Surge SFM can more efficiently expand keratinocytes and properly form stratified epidermis. It also retains keratin-19-positive epithelial stem cells and maintains similar gene expression as with the conventional medium. Surge SFM can be used to produce bilayered self-assembled skin substitutes histologically similar to those produced using serum-containing medium, which can persist for up to six months when grafted onto mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jinmei Wang, Xiaoxiao Wang, Jundong Xie, Bin Yao, Miaohua Mo, Danjun Ma, Chen Huang, Renhe Xu, Xiaobing Fu, Edward E. Tredget, Yaojiong Wu
Summary: SKPs can serve as the hair-inductive cells in engineered skin substitutes, providing them with the potential for hair follicle regeneration.
Article
Biotechnology & Applied Microbiology
Qianghua Quan, Ding Weng, Xuan Li, Quan An, Yang Yang, Bowen Yu, Yuan Ma, Jiadao Wang
Summary: The study developed a biomimetic interface-controlled skin-on-chip system (IC-SoC) by integrating skin extracellular matrix and skin cells into a microfluidic chip. This system provided a stable air-liquid interface (ALI) and mechanical signals for the development of human skin equivalents. The results showed that the skin tissue formed in the microfluidic system had improved barrier function and could be used to simulate bacterial-infected damaged skin. Moreover, the IC-SoC proved effective in evaluating the repair effect of polyphyllin H on the skin barrier and its inhibition effect on inflammation compared to dexamethasone.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell & Tissue Engineering
Wan Tai Seet, Mohd Asyraf Mat Afandi, Mohamad Fikeri Ishak, Muhammad Najib Fathi Hassan, Nazeha Ahmat, Min Hwei Ng, Manira Maarof
Summary: The field of skin injury treatments has advanced significantly in recent years, with advancements in transplants and skin substitutes. This paper discusses the importance of quality assurance and quality control in the manufacturing process of cell and tissue products, using MyDerm (R) as an example. The implementation of standard operating procedures, verification and testing of starting materials, and quality control testing of final products are crucial for ensuring safety and efficacy. Personnel training, environmental monitoring, and risk management are also key aspects.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Engineering, Biomedical
Huijuan Fu, Dequan Zhang, Jinshi Zeng, Qiang Fu, Zhaoyang Chen, Xuer Sun, Yi Yang, Shiyi Li, Minliang Chen
Summary: This study aimed to explore the in vivo application of a 3D-printed tissue-engineered skin substitute loaded with human adipose-derived stem cells (hADSCs) in wound healing. The newly designed biomaterial, composed of adipose tissue decellularized extracellular matrix (dECM) pre-gel, methacrylated gelatin (GelMA), and methacrylated hyaluronic acid (HAMA), was fabricated by 3D printing. The results showed that the 3D-printed skin substitute accelerated wound healing, promoting blood perfusion, re-epithelialization, collagen deposition, and angiogenesis.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Engineering, Biomedical
Yujian Liu, Kaifu Zheng, Zijie Meng, Lei Wang, Xi Liu, Baolin Guo, Jiankang He, Xiyang Tang, Mingyao Liu, Nan Ma, Xiaofei Li, Jinbo Zhao
Summary: In this study, a cell-free tissue-engineered tracheal substitute was developed using a 3D-printed polycaprolactone scaffold coated with a gelatin-methacryloyl hydrogel. Transforming growth factor-β1 (TGF-β) and stromal cell-derived factor-1α (SDF-1) were embedded in the scaffold to facilitate cell recruitment and differentiation. The tracheal substitute showed positive results in promoting the recruitment of cells and enhancing differentiation towards a chondrocyte phenotype.
Article
Multidisciplinary Sciences
Christophe Caneparo, Stephane Chabaud, Julie Fradette, Stephane Bolduc
Summary: This study produced and characterized substitutes for urethral tissue using tissue engineering. By optimizing the mechanical resistance and functionality, the substitutes showed comparable mechanical resistance to human bladder tissue. These substitutes have sufficient mechanical resistance and functionality, making them a potential alternative for future treatments.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Laura von Schledorn, David Puertollano Martin, Nicole Cleve, Janina Zoellner, Doris Roth, Ben Ole Staar, Jan Hegermann, Felix C. Ringshausen, Janna Nawroth, Ulrich Martin, Ruth Olmer
Summary: Primary ciliary dyskinesia (PCD) is a rare genetic disorder that affects the function of motile cilia in the respiratory tract, leading to chronic inflammation and lung damage. Current treatment options for PCD are only symptomatic, highlighting the need for curative therapies. In this study, researchers developed an in vitro model using human induced pluripotent stem cells (hiPSC) to recapitulate the diseased phenotype of PCD at the molecular, structural, and functional levels.
Article
Physiology
Leenah Abugisisa, Emily X. Royse, Matthew W. Kemp, Alan H. Jobe, Noah H. Hillman
Summary: Mechanical ventilation alters the responses of preterm airway epithelium to stimulation in culture. Preterm respiratory epithelial cells respond to LPS and IL-13 similar to adults, but mechanical ventilation changes the response to LPS. BALF from mechanically ventilated animals also alters the response to LPS in both preterm and adult cells.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Article
Engineering, Biomedical
Fabien Kawecki, Dominique Mayrand, Akram Ayoub, Emilie Attiogbe, Lucie Germain, Francois A. Auger, Veronique J. Moulin
Summary: The study focuses on developing a large-sized self-assembled skin substitute as a new solution for covering extensive skin wounds. Experimental results showed that large-sized and standard-sized self-assembled skin substitutes exhibited similar characteristics in vitro and produced similar histological results after grafting.
BIOMEDICAL MATERIALS
(2021)
Article
Toxicology
Shaorui Ke, Qi Liu, Xinlian Zhang, Yuhan Yao, Xudong Yang, Guodong Sui
Summary: This study developed a novel ALI-DC platform to investigate the cytotoxicity and molecular mechanisms of biomass combustion-derived particles in human lung cells. The results showed that the ALI-DC platform was more sensitive compared to traditional submerged culture methods, with BCSCs causing decreased cell viability, increased apoptosis rate, and damage to mitochondria and the p53 pathway in HPAEpiC.
PARTICLE AND FIBRE TOXICOLOGY
(2021)
Article
Biochemical Research Methods
Michael Karimov, Marlene Scherer, Heike Franke, Alexander Ewe, Achim Aigner
Summary: A three-dimensional tissue slice air-liquid interface (ALI) culture model was developed for nanoparticle testing, which showed clearer differences in transfection efficacy and cytotoxicity compared to 2D cell culture. This model allows for the evaluation of biological nanoparticle efficacy and biocompatibility in an intact tissue environment, providing a cost-effective and time-saving alternative.
BIOTECHNOLOGY JOURNAL
(2023)
