Article
Chemistry, Multidisciplinary
Deogil Kim, Byung-Hyun Cha, Jinsung Ahn, Yoshie Arai, Bogyu Choi, Soo-Hong Lee
Summary: Understanding the biophysical relationships between stem cells and applied biomaterials is crucial in controlling stem cell functions. This study highlights the significant role of 3D microenvironment, particularly methacrylated hyaluronic acid (HA) hydrogel, in improving cellular reprogramming into iPSCs. The incorporation of cues from the 3D microenvironment accelerates the reprogramming process, offering potential advantages for translational applications.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Article
Biochemistry & Molecular Biology
Yue Su, Ling Wang, Zhiqiang Fan, Ying Liu, Jiaqi Zhu, Deborah Kaback, Julia Oudiz, Tayler Patrick, Siu Pok Yee, Xiuchun (Cindy) Tian, Irina Polejaeva, Young Tang
Summary: This study successfully generated biPSCs with long-term self-renewal capacity and pluripotent characteristics from bovine mesenchymal stem cells. The cells exhibited morphological changes under different culture conditions, and transcriptome analysis showed high similarity to bovine ESCs and embryos. The naive-like biPSCs also demonstrated the ability to integrate into extra-embryonic tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Hong Cao, Qian Zhou, Chungeng Liu, Yecen Zhang, Minghui Xie, Weihua Qiao, Nianguo Dong
Summary: This study investigates the effect of substrate stiffness on the differentiation of human-induced pluripotent stem cells (h-iPSCs) into valve endothelial-like cells (h-iVECs). The results show that moderate substrate stiffness can enhance the differentiation efficiency of h-iVECs and is regulated by the WNT/Calcineurin signaling pathway. This study emphasizes the importance of using substrate stiffness to achieve a more specific and mature differentiation of h-iVECs.
ACTA BIOMATERIALIA
(2022)
Review
Biochemistry & Molecular Biology
Tianyuan Shi, Martin Cheung
Summary: Using rodents for modeling neurological diseases has limitations due to differences in genetics, neural development, and physiology between humans and rodents. In the past decade, induced pluripotent stem cells and induced neural stem cells generated through reprogramming somatic cells have offered a powerful alternative for studying disease pathogenesis and testing regenerative medicines. Urine-derived stem cells are an ideal cell source for reprogramming due to their proliferation, multipotency, epithelial nature, and ease of reprogramming.
CELL AND BIOSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Samantha E. Spellicy, David C. Hess
Summary: Inflammation is a key contributing factor to the pathogenesis of stroke, leading to increased oxidative stress and neuronal cell death. Recent research has shown that induced pluripotent stem cells have the potential to suppress neuroinflammation, mainly by modulating inflammatory signaling and endogenous inflammatory cell populations.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Dwaipayan Adhya, Vivek Swarup, Roland Nagy, Lucia Dutan, Carole Shum, Eva P. Valencia-Alarcon, Kamila Maria Jozwik, Maria Andreina Mendez, Jamie Horder, Eva Loth, Paulina Nowosiad, Irene Lee, David Skuse, Frances A. Flinter, Declan Murphy, Grainne McAlonan, Daniel H. Geschwind, Jack Price, Jason Carroll, Deepak P. Srivastava, Simon Baron-Cohen
Summary: The study suggests that autism-iPSCs diverge from control-iPSCs at a cellular level during early neurodevelopment, indicating unique developmental differences associated with autism may be established at early prenatal stages.
BIOLOGICAL PSYCHIATRY
(2021)
Review
Cell & Tissue Engineering
Azam Shamsian, Roxana Sahebnasagh, Amir Norouzy, Safin Hassan Hussein, Mohammad Hossein Ghahremani, Zahra Azizi
Summary: Induced pluripotent stem cells (iPSCs) have wide-ranging potential applications in medical research, and cancer cells may serve as a new source for generating iPSCs. Although cancer cells share similarities with iPSCs, they carry genetic abnormalities and pose a risk of tumor formation. This review discusses the feasibility of using cancer cells as a preferred source for iPSC generation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Claudia Bernecker, Eva Maria Matzhold, Dagmar Kolb, Afrim Avdili, Lisa Rohrhofer, Annika Lampl, Martin Troetzmueller, Heike Singer, Johannes Oldenburg, Peter Schlenke, Isabel Dorn
Summary: Functional characterization of cultured red blood cells from human induced pluripotent stem cells (cRBC_iPSCs) was conducted in this study. The results showed that cRBC_iPSCs have similar morphology and maturity stage to native red blood cells, highlighting their potential for future applications.
Review
Biochemistry & Molecular Biology
Isabelle Xavier Dias, Aline Cordeiro, Joao Antonio Matheus Guimaraes, Karina Ribeiro Silva
Summary: The burden of musculoskeletal disorders is increasing worldwide and the treatment options are limited. Induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSC) have emerged as an alternative therapeutic approach with a higher potential for treating musculoskeletal disorders.
Review
Neurosciences
Ranran Duan, Yang Gao, Ruya He, Lijun Jing, Yanfei Li, Zhe Gong, Yaobing Yao, Tingting Luan, Chaopeng Zhang, Li Li, Yanjie Jia
Summary: Ischemic stroke is a severe central nervous system disease with high disability and mortality rates. Current therapies like rt-PA and mechanical thrombectomy have limitations, prompting the exploration of new treatments like stem cell transplantation, particularly iPSCs, which show promise in overcoming challenges and offering neuroprotection in stroke treatment.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Cell Biology
Taiki Satoh, Marcelo A. S. Toledo, Janik Boehnke, Kathrin Olschok, Niclas Flosdorf, Katrin Goetz, Caroline Kuestermann, Stephanie Sontag, Kristin Sere, Steffen Koschmieder, Tim H. Bruemmendorf, Nicolas Chatain, Yoh-ichi Tagawa, Martin Zenke
Summary: Dendritic cells (DC) are professional antigen-presenting cells that can be divided into different subsets, with the DC3 subset having proinflammatory properties. Human iPS cell-derived DC3 exhibit similar characteristics to blood DC3 in vitro. The JAK2 V617F mutation enhances DC3 production and biases towards erythrocytes and megakaryocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Asuka Eguchi, Adriana Fernanda G. S. Gonzalez, Sofia I. Torres-Bigio, Kassie Koleckar, Foster Birnbaum, Joe Z. Zhang, Vicky Y. Wang, Joseph C. Wu, Steven E. Artandi, Helen M. Blau
Summary: Duchenne muscular dystrophy (DMD) is a severe muscle wasting disease caused by the lack of dystrophin. Heart failure is the main cause of death in DMD patients. Previous studies have shown that telomere shortening is a characteristic of DMD cardiomyocytes. This study aims to investigate whether preventing telomere attrition is possible in patient-derived induced pluripotent stem cell differentiated cardiomyocytes (iPSC-CMs) and whether preventing telomere shortening affects cardiomyocyte function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell & Tissue Engineering
Ling Liu, Soochi Kim, Matthew T. Buckley, Jaime M. Reyes, Jengmin Kang, Lei Tian, Mingqiang Wang, Alexander Lieu, Michelle Mao, Cristina Rodriguez-Mateo, Heather D. Ishak, Mira Jeong, Joseph C. Wu, Margaret A. Goodell, Anne Brunet, Thomas A. Rando
Summary: Exercise can rejuvenate stem cells and improve tissue regeneration in aging animals. The cellular and molecular changes caused by exercise in various stem cell compartments have not been extensively studied. In this study, young and old mice were subjected to aerobic exercise and a comprehensive single-cell transcriptomic atlas of different stem cell types and their niche cells was generated. The study found that exercise ameliorated inflammatory pathways associated with old age and restored intercellular communication mediated by immune cells within these stem cell compartments. Exercise had a profound impact on the composition and transcriptomic landscape of circulating and tissue-resident immune cells.
Letter
Cardiac & Cardiovascular Systems
Mengcheng Shen, Chun Liu, Shane Rui Zhao, Amit Manhas, Laksshman Sundaram, Mohamed Ameen, Joseph C. Wu
Editorial Material
Cardiac & Cardiovascular Systems
Xuekun Wu, Francesca Vacante, Joseph C. Wu
EUROPEAN HEART JOURNAL
(2023)
Review
Physiology
Angela Zhang, Zhenqin Wu, Eric Wu, Matthew Wu, Michael P. P. Snyder, James Zou, Joseph C. C. Wu
Summary: Artificial intelligence has greatly advanced in the field of health care over the past decade, especially in utilizing physiology data to transform and improve health care. This review explores the impact of past research on the field and identifies future challenges and directions. The focus is on artificial intelligence models, their application in automating health care tasks, increasing access to care, and enhancing capabilities. The review also addresses emerging concerns regarding individual physiology data and the challenges of deploying artificial intelligence models for meaningful clinical impact.
PHYSIOLOGICAL REVIEWS
(2023)
Article
Cell & Tissue Engineering
Xiaohui Kong, Nadjet Belbachir, Wenshu Zeng, Christopher D. Yan, Sai Navada, Marco V. Perez, Joseph C. Wu
Summary: Catecholaminergic polymorphic ventricular tachycardia is a congenital arrhythmic syndrome caused by mutations on the RYR2 gene. Two patient-specific iPSC lines with RYR2 mutations were generated and evaluated for pluripotency, differentiation capability, and karyotype stability. These iPSC lines provide a valuable tool for studying the CPVT phenotype and underlying mechanisms.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Peter-James H. Zushin, Yang Zhou, Audrey Li, Euan A. Ashley, Matthew T. Wheeler, Joseph C. Wu
Summary: Dilated cardiomyopathies (DCM) are a leading cause of heart failure in aging populations. The chaperone protein BAG3 plays a significant role in the development of DCM and the rate at which it progresses to heart failure. In this study, two human induced pluripotent stem cell (iPSC) lines were generated from individuals with mutations in exon 3 of the BAG3 gene and their pluripotency and ability to differentiate into three primary germ layers were validated. These cell lines serve as a valuable model for studying BAG3 inactivation and insufficiency in relation to DCM.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Wenshu Zenga, Xiaohui Kong, Christina Alamanaa, Yu Liu, Jessica Guzman, Paul D. Pang, John W. Day, Joseph C. Wu
Summary: Spinal muscular atrophy (SMA) is a severe neurodegenerative muscular disease caused by the loss of survival of motor neuron 1 (SMN1) genes. Two iPSC lines were generated from SMA type I patients with SMN1 mutations, and their pluripotency and ability to differentiate into three germ layers were validated. These iPSC lines can be used to generate skeletal muscles to model the muscle atrophy of SMA, providing a complementary platform for in vitro drug screening.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Wenqiang Liu, Wenshu Zeng, Xiaohui Kong, Min Htet, Rebecca Yu, Matthew Wheeler, John W. Day, Joseph C. Wu
Summary: Researchers successfully generated two DMD induced pluripotent stem cell lines, which not only had pluripotency but also the capability to differentiate into cells of the three germ layers in the body. These cell lines can be used to model DMD in vitro and have the potential for therapeutic development.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Wenjuan Zhu, Wenqiang Liu, Rebecca Yu, Melanie Manning, Anitra Waran Romfh, Joseph C. Wu
Summary: This study has successfully generated two induced pluripotent stem cell lines from two patients with Down syndrome, providing valuable resources for research and testing therapeutic strategies for this disorder.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Mao Zhang, Ravichandra Venkateshappa, Audrey Li, Michael B. Fowler, Melinda L. Telli, Joseph C. Wu
Summary: Researchers generated induced pluripotent stem cell (iPSC) lines from blood cells of breast cancer patients with germline ATM mutations. These patient-specific iPSC lines can differentiate into three germ layers and hold great potential for studying mechanisms and developing drug screening strategies for ATM-related cancer.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Mao Zhang, Wenqiang Liu, Audrey Li, Min H. Htet, Rebecca Yu, Melinda L. Telli, Joseph C. Wu
Summary: Germline pathogenic variants in the BRCA2 gene are strongly associated with increased risk of breast cancer. iPSC lines derived from breast cancer patients carrying specific BRCA2 variants demonstrate pluripotency and differentiation potential. These iPSC lines offer valuable resources for personalized pre-clinical research and provide new opportunities for investigating breast cancer mechanisms and targeted therapeutic approaches.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Bernardo Bonilauri, Hye Sook Shin, Min Htet, Christopher D. Yan, Ronald M., Karim Sallam, Joseph C. Wu
Summary: This study generated induced pluripotent stem cell lines from patients with Transthyretin Cardiac Amyloidosis, allowing investigation of the pathological mechanisms related to the disease.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Gaoxian Chen, Lorena Orozco, Sophia Parmisano, James W. S. Jahng, Carlos D. Vera, Yan Zhuge, Joseph C. Wu, Detlef Obal
Summary: This study reports two iPSC lines generated from IPAH patients, which provide valuable research tools for studying the molecular and cellular mechanisms of IPAH.
STEM CELL RESEARCH
(2023)
Letter
Cardiac & Cardiovascular Systems
Nadjet Belbachir, Yiyang Wu, Mengcheng Shen, Sophia L. Zhang, Joe Z. Zhang, Chun Liu, Bjorn C. Knollmann, Gholson J. Lyon, Ning Ma, Joseph C. Wu