Article
Biochemistry & Molecular Biology
Ruonan Zhang, Jun-Ichiro Jo, Ryuhei Kanda, Aki Nishiura, Yoshiya Hashimoto, Naoyuki Matsumoto
Summary: The study modified the surface of polyetheretherketone (PEEK) with sandblasting and polydopamine (PDA) to incorporate a gelatin hydrogel, enabling sustained release of growth factors and osteogenic differentiation of stem cells. The success of gelatin hydrogel binding and degradation with PEEK (GPEEK) was confirmed, along with mineralization in simulated body fluid. The sustained release of bone morphogenetic protein (BMP)-2 in GPEEK led to osteogenic differentiation of human mesenchymal stem cells (hMSCs). PEEK with a gelatin hydrogel incorporating BMP-2 shows promise as a substrate for bone tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Wytrwal, Malgorzata Sekula-Stryjewska, Agata Pomorska, Ewa Oclon, Ewa Zuba-Surma, Szczepan Zapotoczny, Krzysztof Szczubialka
Summary: This study investigated the covalent binding of recombinant human bone morphogenetic protein-2 and -7 in stem cells and its effects on cell adhesion, migration, and osteogenesis. The presence of both proteins enhanced cell adhesion but limited cell motility, while the presence of single proteins promoted cell migration activity. Additionally, the coexistence of the proteins significantly increased early osteogenic marker expression.
Article
Pharmacology & Pharmacy
Chih-Hsin Cheng, Ya-Shun Chen, Hsu -Ting Chang, Kai -Chi Chang, Ssu-Meng Huang, Shih-Ming Liu, Wen-Cheng Chen
Summary: This study aimed to develop biodegradable millimeter-scale spherical hydrogel beads with highly interconnected pores that can effectively serve dual functions for cell growth and antibiotic release. Sodium alginate and gelatin were used as the matrix, which were synergistically cross-linked using different ratios of cross-linkers. The physicochemical properties of the beads were measured, and gentamicin-impregnated hydrogel beads showed better antibacterial activity compared to hydrogel without antibiotics. The developed hydrogel beads have good antibacterial activity, and can be used in combination with different medical devices to improve biocompatibility and reduce the risk of surgical failure caused by infection.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Engineering, Biomedical
Sarah A. Schoonraad, Alan A. Jaimes, Arjun J. X. Singh, Kiera J. Croland, Stephanie J. Bryant
Summary: This study investigates the osteogenic effects of tethered BMP-2 and BMP-9 on human MSCs encapsulated in a hydrogel. The results show that tethered BMP-2 and BMP-9 both effectively enhance osteogenesis of MSCs, leading to a mature osteoblast phenotype.
ACTA BIOMATERIALIA
(2023)
Article
Materials Science, Biomaterials
M. C. Echave, I Erezuma, N. Golafshan, M. Castilho, F. B. Kadumudi, C. Pimenta-Lopes, F. Ventura, A. Pujol, J. J. Jimenez, J. A. Camara, R. Hernaez-Moya, L. Iturriaga, L. Saenz Del Burgo, I. Iloro, M. Azkargorta, F. Elortza, R. Lakshminarayanan, T. H. Al-Tel, P. Garcia-Garcia, R. Reyes, A. Delgado, C. Evora, J. L. Pedraz, A. Dolatshahi-Pirouz, G. Orive
Summary: In this study, a new osteoconductive scaffold was developed, which reduced the required doses of BMP-2 for bone regeneration. The scaffold showed promising results in promoting cell adhesion, proliferation, and osteogenic differentiation of human bone-marrow derived mesenchymal stem cells (hBM-MSCs). The sustained release of BMP-2 from the scaffold allowed for the treatment of critical-sized osteoporotic bone defects with relatively low growth factor doses.
BIOMATERIALS ADVANCES
(2022)
Article
Biotechnology & Applied Microbiology
Ji Won Baek, Ki Su Kim, Ho Park, Nak Gyu Park, Beom-Su Kim
Summary: In this study, we improved the osteoinductive activity of marine-plankton-derived whitlockite (MP-WH) granules by incorporating bone morphogenetic protein2 (BMP2). The results showed that hMSCs seeded on MP-WH/BMP2 granules exhibited better biocompatibility, proliferation, and adhesion compared to MP-WH. Furthermore, the incorporation of BMP2 significantly upregulated the expression of bone-specific markers, indicating enhanced osteogenic effects.
BIOENGINEERING-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Hyun Seok, Hee-Youl Kim, Dong-Cheol Kang, Jung-Ho Park, Jong Hoon Park
Summary: The study compared the bone regeneration ability of bone scaffolds using particle and block bones with rhBMP-2, showing that rhBMP-2 with block bones resulted in significantly higher bone volume but also led to the formation of adipose tissue and void spaces.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Jonathan Dorogin, Henry B. B. Hochstatter, Samantha O. O. Shepherd, Justin E. E. Svendsen, Morrhyssey A. A. Benz, Andrew C. C. Powers, Karly M. M. Fear, Jakob M. M. Townsend, James S. S. Prell, Parisa Hosseinzadeh, Marian H. H. Hettiaratchi
Summary: By using yeast surface display, unique BMP-2-specific affibodies were identified to modulate the release and activity of BMP-2, offering a promising approach for controlling BMP-2 delivery in clinical applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Medicinal
Eleni K. Tsekoura, Teo Dick, Porntipa Pankongadisak, Daniel Graf, Yaman Boluk, Hasan Uludag
Summary: This study investigated the delivery of gene complexes via electrospun mats with different compositions to cells, finding that a high fraction of collagen in the mats can affect fiber quality and reduce transfection efficiency, while double-layered mats containing collagen without complexes as a first layer and gelatin-collagen-PEG with complexes as a second layer successfully induced GFP expression in cells.
Article
Engineering, Biomedical
Sihan Lin, Shi Yin, Junfeng Shi, Guangzheng Yang, Xutao Wen, Wenjie Zhang, Mingliang Zhou, Xinquan Jiang
Summary: In this study, the dose of bone morphogenetic protein-2 (BMP-2) was reduced and its safety and efficacy were improved through the regulation of magnesium ion (Mg2+). Magnesium ion enhanced cellular bioenergetic levels and the osteoinductivity of BMP-2. A co-delivery system, fabricated using microfluidic and 3D printing technologies, was able to rapidly and robustly induce bone regeneration.
BIOACTIVE MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Gaoli Xu, Chenxi Shen, Haiyan Lin, Jian Zhou, Ting Wang, Ben Wan, Munerah Binshabaib, Tymour Forouzanfar, Guochao Xu, Nawal Alharbi, Gang Wu
Summary: This study synthesized a novel biomimetically-precipitated nanocrystalline calcium phosphate (BpNcCaP) with internally incorporated bone morphogenetic protein-2 (BpNcCaP + BMP-2), demonstrating its potential application in repairing large-volume bone defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Cheol Hee Jeong, Song-Yi Lim, Jo Eun Um, Hyo Won Lim, Kyu Ho Hwang, Kyeong-Mee Park, Jun Seop Yun, Dohun Kim, Jong-Ki Huh, Hyun Sil Kim, Jong In Yook, Nam Hee Kim, Yoon Hae Kwak
Summary: This study introduces micellized bone morphogenetic protein-2 (mPTD-BMP-2), a next-generation osteogenic supplement that combines protein transduction domain (PTD) and nano-sized micelle formulation technique to improve transduction efficiency and stability. The use of PTD represents a novel approach, and our results demonstrate the superiority of mPTD-BMP-2 over rh-BMP-2 in terms of in vivo pharmacokinetic profile and osteogenic potential, particularly in a rat tibial model of distraction osteogenesis.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Eun Young Jeon, Seung-Hoon Um, Jaeho Park, Youngmee Jung, Cheol-Hong Cheon, Hojeong Jeon, Justin J. Chung
Summary: In this study, a complex coacervate consisting of fucoidan and poly-l-lysine (PLL) was fabricated to encapsulate BMP-2, avoiding denaturation and enhancing therapeutic action. The coacervate showed superior binding ability to BMP-2 and allowed for prolonged release, improving its bioactivity and protection against antagonist and proteolysis. The coated coacervate on collagen sponges demonstrated bioactivity and localized bone regenerating ability in vitro and in vivo evaluations.
Article
Engineering, Biomedical
Jia Liang, Zhule Wang, Andreas A. Poot, Dirk W. Grijpma, Piet J. Dijkstra, Rong Wang
Summary: It has been found that methacrylated gelatin (GelMA) can be used as a 3D printable scaffold material in tissue regeneration fields, but its long-term stability needs to be improved. A mixture of GelMA and tyramine-conjugated 8-arm PEG (8PEGTA) was used to create filaments with an interpenetrating network (IPN), increasing the shape stability of the printed scaffolds. This new material was demonstrated to be a good candidate for extrusion-based printing of constructs for tissue engineering applications.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Biotechnology & Applied Microbiology
Xianggang Wang, Zhengyan Li, Zhonghan Wang, He Liu, Yutao Cui, Yuzhe Liu, Ming Ren, Hongsheng Zhan, Zuhao Li, Minfei Wu, Jincheng Wang
Summary: The study developed a thermosensitive hydrogel loaded with BMP-2 and OPG to promote bone formation and inhibit osteoclast activity, which was then used to construct 3D-printed composite scaffolds. These scaffolds facilitated osteogenic differentiation, reduced osteoclastic activation, and improved bone ingrowth and osseointegration through sustained release of BMP-2 and OPG.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)