Article
Engineering, Biomedical
Joao F. Crispim, Keita Ito
Summary: This study introduces a new method for quickly mass producing self-assembling cartilage organoids, which are then encapsulated in hydrogels with different viscoelastic properties. The approach optimizes chondrocyte expansion and assembly, demonstrating potential for large scale cartilage regeneration.
ACTA BIOMATERIALIA
(2021)
Article
Polymer Science
Francisco Drusso Martinez-Garcia, Roderick Harold Jan de Hilster, Prashant Kumar Sharma, Theo Borghuis, Machteld Nelly Hylkema, Janette Kay Burgess, Martin Conrad Harmsen
Summary: The study compared the viscoelastic properties of ECM hydrogels derived from the skin, lung, and cardiac left ventricle, showing differences in elastic modulus and relaxation behavior. These differences were found to correlate with the biochemical composition and structure of the ECM hydrogels.
Article
Nanoscience & Nanotechnology
Chengqi Lyu, Chi Cheng, YuShi He, Ling Qiu, Zijun He, Derong Zou, Dan Li, Jiayu Lu
Summary: Porous scaffolds are commonly used for cartilage tissue regeneration, but understanding the impact of their hierarchical structure on the regeneration process can be challenging. In this study, a graphene hydrogel with stable and adjustable structure was used as a model scaffold to investigate the effect of porous structure on matrix remodeling and chondrocyte ingrowth. The results showed that the graphene scaffold with an open pore structure accelerated and balanced cartilage remodeling. Furthermore, this enhanced remodeling selectively promoted the expression of collagen type II fibrils, providing new insights into scaffold design for cartilage repair.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biotechnology & Applied Microbiology
Danni Gong, Fei Yu, Meng Zhou, Wei Dong, Dan Yan, Siyi Zhang, Yan Yan, Huijing Wang, Yao Tan, Ying Chen, Bei Feng, Wei Fu, Yao Fu, Yang Lu
Summary: dcECM hydrogels derived from porcine ears through enzymatic digestion show good biocompatibility for delivering chondrocytes and forming subcutaneous cartilage in vivo. The structural and gelation kinetics of the hydrogels vary with ECM concentrations, with 10 mg/ml hydrogels supporting adhesion and proliferation of chondrocytes in vitro. In vivo, 10 mg/ml dcECM hydrogel grafts exhibit similar qualities to native cartilage, indicating their potential for tissue engineering applications in ear cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Lorena Rivera-Hernandez, Norberto Chavarria-Hernandez, Alberto Tecante, Mayra Alejandra Lopez-Ortega, Ma del Rocio Lopez Cuellar, Adriana-Ines Rodriguez-Hernandez
Summary: This research aimed to analyze the viscoelastic behavior of mixed hydrogels based on low methoxyl citrus pectin (DE = 30.4%) and low acyl gellan. The co-gelification of gellan and pectin provided more rigid hydrogels characterized by narrow meshes and slower rearrangements of polymer chains compared to gellan gels. The viscoelastic behavior of the pectin-gellan gels suggests a macromolecular network organization where gellan junction zones predominate, and pectin contributes to reinforcing the mixed network.
FOOD HYDROCOLLOIDS
(2023)
Review
Biochemistry & Molecular Biology
Wenguang Wang, Yanbin Shi, Guimei Lin, Bingtao Tang, Xuelin Li, Jing Zhang, Xinbing Ding, Guangzhen Zhou
Summary: This paper discusses the application of hydrogels in cartilage repair, including their mechanical properties and materials used. The challenges faced by hydrogels and future research directions are also discussed.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Multidisciplinary Sciences
Maobin Xie, Liming Lian, Xuan Mu, Zeyu Luo, Carlos Ezio Garciamendez-Mijares, Zhenrui Zhang, Arturo Lopez, Jennifer Manriquez, Xiao Kuang, Junqi Wu, Jugal Kishore Sahoo, Federico Zertuche Gonzalez, Gang Li, Guosheng Tang, Sushila Maharjan, Jie Guo, David L. Kaplan, Yu Shrike Zhang
Summary: This study demonstrates the rapid volumetric printing of unmodified silk sericin and silk fibroin inks, which exhibit shape-memory and tuneable mechanical properties. The inks are cytocompatible, providing expanded bioink libraries for volumetric additive manufacturing and offering potential for diverse biomedical applications.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Elizabeth R. Kahle, Biao Han, Prashant Chandrasekaran, Evan R. Phillips, Mary K. Mulcahey, X. Lucas Lu, Michele S. Marcolongo, Lin Han
Summary: Molecular engineering using synthetic mimics of native matrix molecules can modulate the mechanical properties of cellular microenvironment and influence cell mechanobiology. This study demonstrated the use of biomimetic proteoglycans (BPGs) to engineer the micromechanics of the pericellular matrix (PCM) in cartilage, leading to enhanced cellular mechanotransduction. The interactions between BPGs and the native PCM were facilitated by the biomimetic ultrastructure of BPGs, and showed potential for improving tissue regeneration and disease modification in various cell types.
Article
Biotechnology & Applied Microbiology
Donglei Liu, Hao Zhang, Xufeng Dong, Lin Sang, Min Qi
Summary: Cartilage tissue engineering technology provides a solution for treating osteoarthritis. In this study, cellulose nanocrystal/collagen hydrogels with tunable viscoelastic properties were used as a viscoelastic microenvironment to investigate the effects on chondrocyte behaviors. The results showed that enhancing the hydrogel's viscoelastic properties promoted cell shape change, proliferation, and matrix deposition, and reduced inflammation levels. Stress relaxation was identified as the factor with the strongest correlation to chondrocyte behaviors. FAK and YAP were also found to be involved in the chondrocytes' response to the hydrogel.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Stanislas Le Guisquet, Marco Amabili
Summary: This study characterizes the viscoelastic properties of a hydrogel in sol-gel transition by measuring the vibration response of a rigid circular cylindrical container's bottom silicone membrane. A finite-element numerical model was built for identification of the hydrogel's storage shear modulus and loss tangent. It was found that membrane vibration can be used to characterize the viscoelastic mechanical properties of a hydrogel in sol-gel transition in a more versatile way.
JOURNAL OF THE MECHANICS AND PHYSICS OF SOLIDS
(2023)
Article
Construction & Building Technology
Dengwu Jiao, Geert De Schutter
Summary: Understanding the viscoelastic properties of cement paste is important for various applications of cement-based materials. This paper investigates the viscoelastic properties of cement paste with different compositions and provides new insights. The results show that increasing water-to-cement ratio decreases the storage modulus and viscoelastic yield stress, while increasing critical strain. The addition of nano-Fe3O4 particles enhances the storage modulus, critical strain, and viscoelastic yield stress. The concentration of polycarboxylate ether superplasticizer affects the critical strain and viscoelastic yield stress of cement paste.
CONSTRUCTION AND BUILDING MATERIALS
(2022)
Article
Engineering, Biomedical
Zhixiang Cai, Yanmei Tang, Yue Wei, Pengguang Wang, Hongbin Zhang
Summary: In this study, a double-network hydrogel based on hyaluronan and gellan gum was fabricated, which exhibited high compressive strength, high toughness, stiffness, and good self-recovery property. The hydrogel could support chondrocyte proliferation and new extracellular matrix deposition, correlated with its improved mechanical properties, good cytocompatibility, and biodegradability. In vivo experiments in animals demonstrated that this hydrogel facilitated hyaline-like cartilage regeneration. These findings suggest that the developed hydrogel as a biomimetic extracellular matrix offers a hopeful new platform for cartilage tissue engineering.
ACTA BIOMATERIALIA
(2022)
Article
Biology
Richard Gerum, Elham Mirzahossein, Mar Eroles, Jennifer Elsterer, Astrid Mainka, Andreas Bauer, Selina Sonntag, Alexander Winterl, Johannes Bartl, Lena Fischer, Shada Abuhattum, Ruchi Goswami, Salvatore Girardo, Jochen Guck, Stefan Schruefer, Nadine Stroehlein, Mojtaba Nosratlo, Harald Herrmann, Dorothea Schultheis, Felix Rico, Sebastian Johannes Mueller, Stephan Gekle, Ben Fabry
Summary: This study presents a high-throughput, simple, and low-cost microfluidic method to quantitatively measure the elastic and viscous moduli of individual cells. By measuring cell deformation and rotation frequency, the frequency-dependent viscoelastic properties of cells can be determined, revealing the influence of cell cycle and physical interactions between different cell structures.
Article
Chemistry, Applied
Suping Chen, Chengheng Wu, Ting Zhou, Kai Wu, Nini Xin, Xiaoyin Liu, Zi Qiao, Dan Wei, Jing Sun, Hongrong Luo, Liangxue Zhou, Hongsong Fan
Summary: In this study, we developed a hydrogel system with orthogonal viscoelasticity and nano-topography by modifying hyaluronan. The matrix nanoorientation and viscoelasticity were found to have positive effects on neurites outgrowth, neural cell elongation, and neuronal differentiation. Moreover, implantation of the viscoelastic and nano-aligned hydrogels showed enhanced repair of spinal cord injury, including improved pathological microenvironment, facilitated endogenous neurogenesis, axon regeneration, and motor function restoration.
CARBOHYDRATE POLYMERS
(2022)
Article
Engineering, Multidisciplinary
Yijian Zhang, Weiling Yin, Yang Liu, Mingzhuang Hou, Qin Shi, Tao Liu, Miao Wang, Huilin Yang, Guoqing Pan, Fan He, Xuesong Zhu
Summary: By fabricating a dynamic hydrogel based on the coordination between metal ions and collagen and enveloping it with the chondro-inductive factor kartogenin (KGN) via cyclodextrin (CD), this study demonstrated that the hydrogel can promote cartilage matrix expression and enhance chondrocyte mitochondrial function and intracellular antioxidant properties, thereby benefiting cartilage repair and reconstruction.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Pathology
Chenshuang Li, Zhong Zheng, Jie Jiang, Wenlu Jiang, Kevin Lee, Emily A. Berthiaume, Eric C. Chen, Cymbeline T. Culiat, Yan-Heng Zhou, Xinli Zhang, Kang Ting, Chia Soo
AMERICAN JOURNAL OF PATHOLOGY
(2018)
Article
Endocrinology & Metabolism
Gexin Zhao, Bau-Lin Huang, Diana Rigueur, Weiguang Wang, Chimay Bhoot, Kemberly R. Charles, Jongseung Baek, Subburaman Mohan, Jie Jiang, Karen M. Lyons
JOURNAL OF BONE AND MINERAL RESEARCH
(2018)
Article
Cell Biology
Jie Jiang, Gexin Zhao, Karen M. Lyons
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2018)
Correction
Cell Biology
Jie Jiang, Gexin Zhao, Karen M. Lyons
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2018)
Review
Cell Biology
Kathryn E. Worley, Jennifer Rico-Varela, Dominic Ho, Leo Q. Wan
INTEGRATIVE BIOLOGY
(2018)
Article
Cell & Tissue Engineering
Ahmad E. Abu-Hakmeh, Allison K. M. Fleck, Leo Q. Wan
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2019)
Article
Orthopedics
Natalie L. Leong, Jamie L. Kator, Thomas L. Clemens, Aaron James, Motomi Enamoto-Iwamoto, Jie Jiang
JOURNAL OF ORTHOPAEDIC RESEARCH
(2020)
Article
Engineering, Biomedical
Chenshuang Li, Zhong Zheng, Pin Ha, Wenlu Jiang, Emily A. Berthiaume, Seungjun Lee, Zane Mills, Hsinchuan Pan, Eric C. Chen, Jie Jiang, Cymbeline T. Culiat, Xinli Zhang, Kang Ting, Chia Soo
Article
Endocrinology & Metabolism
Gexin Zhao, Elliot W. Kim, Jie Jiang, Chimay Bhoot, Kemberly R. Charles, Jongseung Baek, Subburaman Mohan, John S. Adams, Sotirios Tetradis, Karen M. Lyons
JOURNAL OF BONE AND MINERAL RESEARCH
(2020)
Article
Cell & Tissue Engineering
Haokang Zhang, Jie Fan, Zhen Zhao, Chunyu Wang, Leo Q. Wan
Summary: Cell chirality plays a role in determining the directionality of cellular polarization. The study found that tau protein may disrupt cell polarization and chiral morphogenesis, potentially leading to blood-brain barrier dysfunction in Alzheimer's disease. Meanwhile, the effects of ApoE4 and A beta-42 on BBB integrity appear to be independent of cell chirality.
CELLULAR AND MOLECULAR BIOENGINEERING
(2021)
Review
Cell & Tissue Engineering
Guohao Dai, Adam W. Feinberg, Leo Q. Wan
Summary: This report summarizes the theme and goal of the BMES-CMBE conference held in January 2020 in Puerto Rico, as well as the topics discussed and expectations presented.
CELLULAR AND MOLECULAR BIOENGINEERING
(2021)
Article
Biophysics
Tasnif Rahman, Frank Peters, Leo Q. Wan
Summary: A cell vertex model is used to study the role of tissue rigidity or jamming state on chiral morphogenesis. It is found that solid-like tissues exhibit stronger chiral cell alignment and elongation compared to fluid-like tissues. The study reveals the role of chiral torque in biased cellular alignment and the importance of cell density-independent tissue rigidity in this process.
JOURNAL OF BIOMECHANICS
(2023)
Article
Engineering, Biomedical
Benson Hang, Eman Jassem, Hanan Mohammed, Leo Q. Wan, Jason Herschkowitz, Jie Fan
Summary: Physical interaction between tumor cells and endothelial cells weakens the rotational characteristics of endothelial cells, while paracrine signaling has minimal impact. Disruption of EC chirality may increase the risk of metastatic cancer spread.
APL BIOENGINEERING
(2022)
Article
Materials Science, Biomaterials
Haokang Zhang, Jie Fan, Joshua M. A. Maclin, Leo Q. Wan
Summary: The left-right asymmetry of cells is an intrinsic property derived from the dynamic organization of the actin cytoskeleton. Actin-binding proteins and signaling pathways, such as PKC, can regulate cell chirality. Additionally, fascin, an actin crosslinker, plays a critical role in cell chirality regulation by facilitating cell migration.
Review
Engineering, Biomedical
Tasnif Rahman, Haokang Zhang, Jie Fan, Leo Q. Wan
APL BIOENGINEERING
(2020)