Article
Multidisciplinary Sciences
Yuuki Shirai, Junko Okano, Takahiko Nakagawa, Miwako Katagi, Yuki Nakae, Atsuhiro Arakawa, Shinya Koshinuma, Gaku Yamamoto, Hideto Kojima
Summary: This article investigates the regenerating effect of gelatin sponges as scaffolds in deep wounds with periosteal defects in rats. The study demonstrates that the microenvironment provided by the gelatin sponges enables bone marrow-derived cells to form a vascular niche, leading to the regeneration of periosteum, skin, and skin appendages. These findings emphasize the importance of vascular niche formation in the regeneration of heterogeneous tissues in deep wounds.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Biomedical
Jonathan H. Galarraga, Hannah M. Zlotnick, Ryan C. Locke, Sachin Gupta, Natalie L. Fogarty, Kendall M. Masada, Brendan D. Stoeckl, Lorielle Laforest, Miguel Castilho, Jos Malda, Riccardo Levato, James L. Carey, Robert L. Mauck, Jason A. Burdick
Summary: Soft norbornene-modified hyaluronic acid (NorHA) hydrogels were reinforced with polycaprolactone using melt electrowriting (MEW) to fabricate composite scaffolds. These scaffolds supported chondrogenesis and cartilage formation of encapsulated porcine mesenchymal stromal cells (pMSC) and exhibited suitable mechanical properties. Fixation of the composites with biodegradable pins or fibrin glue ensured implant retention, but the use of pins resulted in inferior cartilage repair compared to the use of glue.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Materials Science, Biomaterials
Bing Guo, Xiaodong Feng, Yun Wang, Xiansong Wang, Yue He
Summary: The study evaluated a biomimetic baicalin-incorporating graphene oxide-demineralized bone matrix hybrid scaffold for bone regeneration. It demonstrated that the scaffold enhanced beneficial cross-talk among bone cells and inflammatory cells, which could be utilized as an effective strategy for bone regeneration. Additionally, the scaffold showed potential in transforming inflammatory M1 macrophages into pro-healing M2 macrophages, promoting in vitro angiogenesis and osteogenesis.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Engineering, Biomedical
Juliana Giron Bastidas, Natasha Maurmann, Luiza Oliveira, Bruno Alcantara, Camila Vieira Pinheiro, Guilhian Leipnitz, Fabiola Meyer, Maikel Oliveira, Paula Rigon, Patricia Pranke
Summary: The aim of this study was to analyze a bilayer scaffold of poly(lactide-co-glycolide)/fibrin electrospun membrane and fibrin hydrogel layer in vivo on a rat skin model. The results showed that the scaffold promoted granulation tissue growth, collagen deposition, and epithelial tissue remodeling.
BIOMEDICAL MATERIALS
(2023)
Article
Engineering, Biomedical
Thakoon Thitiset, Siriporn Damrongsakkul, Supansa Yodmuang, Wilairat Leeanansaksiri, Jirun Apinun, Sittisak Honsawek
Summary: The novel biodegradable scaffold comprising gelatin (G), chitooligosaccharide (COS), and demineralized bone matrix (DBM) showed promising biological characteristics for bone tissue engineering both in vitro cell culture and in vivo animal bioassays.
BIOMATERIALS RESEARCH
(2021)
Article
Polymer Science
Banafsheh Safari, Marziyeh Aghazadeh, Leila Roshangar, Ayuob Aghanejad, Soodabeh Davaran
Summary: This study reports the fabrication and evaluation of a bioactive hybrid scaffold collagen/P-PCL for bone tissue engineering. The scaffold showed biocompatibility, osteoconductivity, and osteoinductivity, supporting the proliferation and differentiation of AD-MSCs.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Pharmacology & Pharmacy
Jiafu Mu, Jiahe Wu, Jian Cao, Teng Ma, Liming Li, Shiqing Feng, Jianqing Gao
Summary: This study evaluates an emergency cell-free treatment using mesenchymal stem cell-derived exosomes for traumatic spinal cord injury, which has been shown to comprehensively mitigate the inhibitory lesion microenvironment, providing a promising strategy for effective emergency treatment of central nervous system trauma.
ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sebastian Blatt, Daniel G. E. Thiem, Andreas Pabst, Bilal Al-Nawas, Peer W. Kaemmerer
Summary: The combination of bone substitute materials (BSMs) with platelet-rich fibrin (PRF) showed significant positive pro-angiogenic effects, particularly with alloplastic and xenogeneic materials, leading to increased vessel formation in vivo. This demonstrates the enhanced angiogenetic potential of BSMs when combined with PRF, suggesting potential for bony regeneration in clinical applications.
Article
Medicine, Research & Experimental
Xiaohua Ren, Qingwei Wang, Chunhui Liu, Qian Zhao, Jiajun Zheng, Kun Tian, Huijuan Xu, Yandong Mu
Summary: The study found that the porous HAG scaffold-based delivery of hPMSCs significantly induced osteogenic effect in vivo, promoting the formation of bone plate structure. RNA-seq revealed the involvement of various osteogenesis-related pathways at different metabolic stages, with increased expression of collagen-1 and runt-related transcription factor 2 with implantation time.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Cell & Tissue Engineering
Lvzhong Hu, Jinhua Zhou, Zhisong He, Lin Zhang, Fangzhou Du, Mengting Nie, Yao Zhou, Hang Hao, Lixing Zhang, Shuang Yu, Jingzhong Zhang, Youguo Chen
Summary: The study found that implanting human umbilical cord mesenchymal stem cells (hUC-MSCs) in a fibrin hydrogel scaffold can promote skin repair, prolong cell survival, and accelerate wound healing. The hydrogel scaffold provides a stable environment for cell adhesion, proliferation, and migration, while the combination therapy with hUC-MSCs promotes epithelial regeneration and angiogenesis. It exhibits a remarkable therapeutic effect, more effective than monotherapy with hUC-MSCs or hydrogel.
CELL TRANSPLANTATION
(2023)
Article
Engineering, Biomedical
Shiyu Yao, Yuli Shang, Bo Ren, Shu Deng, Zhe Wang, Yang Peng, Zhaohui Huang, Shiqing Ma, Cheng Peng, Shuai Hou
Summary: This study evaluated the potential use of collagen-based biomaterials from non-mammalian donors in bone repair. Tilapia skin collagen-hydroxyapatite scaffolds were successfully fabricated and showed promising microstructure, chemical, physical, and mechanical properties. In vitro studies demonstrated that the collagen/hydroxyapatite ratio of 1:4 had the most significant effect on the proliferation, differentiation, and mineralization of bone mesenchymal stem cells. In vivo experiments showed that the same ratio of scaffolds had excellent osteogenic activities in rat skull critical defections.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2022)
Article
Multidisciplinary Sciences
Sung Ill Jang, Sungsoon Fang, Ji Hae Nahm, Jae Hee Cho, Min Young Do, Su Yeon Lee, Seok Jeong, Don Haeng Lee, Dong Ki Lee
Summary: This study investigated the safety and efficacy of corticosteroid-eluting fully covered self-expandable metal stents (FCSEMS) in an animal model and found that they had the potential to reduce fibrotic tissue. Further research is needed to confirm their safety and efficacy in humans with refractory benign biliary strictures (BBS).
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Elisa Mazzoni, Chiara Mazziotta, Maria Rosa Iaquinta, Carmen Lanzillotti, Francesca Fortini, Antonio D'Agostino, Lorenzo Trevisiol, Riccardo Nocini, Giovanni Barbanti-Brodano, Andrea Mescola, Andrea Alessandrini, Mauro Tognon, Fernanda Martini
Summary: Human bone marrow-derived mesenchymal stem cells (hBMSCs) and their derivative enhanced green fluorescent protein (eGFP)-hBMSCs were used to evaluate an innovative hybrid scaffold composed of granular hydroxylapatite and collagen hemostat. The scaffold supported cell growth, metabolism, and osteogenic differentiation, indicating its potential for bone repair and tissue engineering applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
He Yang, Zhiqiang Dong, Hongya Wang, Zicheng Liu, Wenbo Sun, Kun Wang, Xinfeng Xu, Wei Wen, Jun Wang, Liang Chen, Quan Zhu
Summary: This study designed a new method using oxidized regenerated cellulose and fibrin glue to cover the intersegmental plane in lung segmentectomy and prevent air leakage. The adhesive strength and effect on reducing air leakage were validated in an excised porcine lung segmentectomy model. The results showed that the combination of fibrin glue and oxidized regenerated cellulose had good sealing performance.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Materials Science, Biomaterials
Qiuping Leng, Zhuo Liang, Yonggang Lv
Summary: Gene therapy based on mRNA holds promise for bone regeneration, with different osteoinductive-mRNAs released by osteogenically pre-differentiated MSCs at various stages showing varied effects on osteogenic differentiation of MSCs. Among them, Oi14-mRNA significantly promoted the expression of specific markers and new bone formation in MSCs.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Cell & Tissue Engineering
Sun-A Ock, Geun-Ho Maeng, Yeon-Mi Lee, Tae-Ho Kim, B. Mohana Kumar, Sung-Lim Lee, Gyu-Jin Rho
CELL TRANSPLANTATION
(2013)
Article
Genetics & Heredity
B. Mohana Kumar, Geun Ho Maeng, Ryoung Hoon Jeon, Yeon Mi Lee, Won Jae Lee, Byeong Gyun Jeon, Sun A. Ock, Gyu Jin Rho
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2012)
Article
Engineering, Biomedical
Jin Ho Lee, Shin-Won Kim, Uk-Kyu Kim, Se Heang Oh, Sang June-Kim, Bong-Wook Park, Jin-Hyun Kim, Young-Sool Hah, Deok Ryong Kim, Gyu-Jin Rho, Geun-Ho Maeng, Ryoung-Hoon Jeon, Hee-Chun Lee, Jong-Ryoul Kim, Gyoo-Cheon Kim, June-Ho Byun
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2013)
Article
Cell & Tissue Engineering
Bong-Wook Park, Dong-Ho Kang, Eun-Ju Kang, June-Ho Byun, Jong-Sil Lee, Geun-Ho Maeng, Gyu-Jin Rho
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2012)
Meeting Abstract
Developmental Biology
J. H. Lee, Y. M. Lee, G. H. Maeng, R. H. Jeon, T. H. Kim, W. J. Lee, S. A. Ock, B. G. Jeon, B. Mohana Kumar, S. L. Lee, G. J. Rho
REPRODUCTION FERTILITY AND DEVELOPMENT
(2012)
Meeting Abstract
Developmental Biology
W. J. Lee, G. H. Maeng, R. H. Jeon, G. J. Rho, S. L. Lee
REPRODUCTION FERTILITY AND DEVELOPMENT
(2012)
Meeting Abstract
Developmental Biology
S. A. Ock, G. H. Maeng, Y. M. Lee, T. H. Kim, B. M. Kumar, S. L. Lee, G. J. Rho
REPRODUCTION FERTILITY AND DEVELOPMENT
(2012)
Meeting Abstract
Developmental Biology
T. H. Kim, Y. M. Lee, G. H. Maeng, J. H. Lee, R. H. Jeon, S. A. Sun, B. Mahana Kumar, B. G. Jeon, W. J. Lee, S. L. Lee, G. J. Rho
REPRODUCTION FERTILITY AND DEVELOPMENT
(2012)
Article
Veterinary Sciences
B. Mohana Kumar, Geun-Ho Maeng, Yeon-Mi Lee, Tae-Ho Kim, Jeong-Hyeon Lee, Byeong-Gyun Jeon, Sun-A Ock, Jae-Gyu Yoo, Gyu-Jin Rho
RESEARCH IN VETERINARY SCIENCE
(2012)
Article
Veterinary Sciences
B. Mohana Kumar, Geun-Ho Maeng, Yeon-Mi Lee, Jeong-Hyeon Lee, Byeong-Gyun Jeon, Sun-A Ock, Taeyoung Kang, Gyu-Jin Rho
VETERINARY RESEARCH COMMUNICATIONS
(2013)
Article
Biotechnology & Applied Microbiology
Satyabrata Das, Naoko Koyano-Nakagawa, Ohad Gafni, Geunho Maeng, Bhairab N. Singh, Tara Rasmussen, Xiaoyan Pan, Kyung-Dal Choi, Daniel Mickelson, Wuming Gong, Pruthvi Pota, Cyprian V. Weaver, Stefan Kren, Jacob H. Hanna, Demetris Yannopoulos, Mary G. Garry, Daniel J. Garry
NATURE BIOTECHNOLOGY
(2020)
Article
Developmental Biology
Geunho Maeng, Wuming Gong, Satyabrata Das, Demetris Yannopoulos, Daniel J. Garry, Mary G. Garry
Article
Engineering, Biomedical
Geunho Maeng, Satyabrata Das, Sarah M. Greising, Wuming Gong, Bhairab N. Singh, Stefan Kren, Daniel Mickelson, Erik Skie, Ohad Gafni, Jacob R. Sorensen, Cyprian V. Weaver, Daniel J. Garry, Mary G. Garry
Summary: Through CRISPR technology, deletion of MYF5, MYOD and MYF6 genes in pig embryos can lead to the production of intraspecies and interspecies chimaeric pig embryos lacking native skeletal muscle, followed by somatic-cell nuclear transfer and the delivery of exogenous cells through blastocyst complementation. The generated chimaeric pig embryos exhibited normal histology, morphology and function, showing potential for research and xenotransplantation of exogenic organs. Furthermore, human:pig chimaeras created with TP53-null human induced pluripotent stem cells demonstrated higher chimaerism efficiency, with humanized muscle observed in embryos at different embryonic stages.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Veterinary Sciences
Ryoung-Hoon Jeon, Geun-Ho Maeng, Won-Jae Lee, Tae-Ho Kim, Yeon-Mi Lee, Jeong-Hyeon Lee, Basavarajappa-Mohana Kumar, Sung-Lim Lee, Gyu-Jin Rho
JAPANESE JOURNAL OF VETERINARY RESEARCH
(2012)
Article
Oncology
Byeong-Gyun Jeon, B. Mohana Kumar, Eun-Ju Kang, Geun-Ho Maeng, Yeon-Mi Lee, Young-Sool Hah, Sun-A Ock, Dae-Oh Kwack, Bong-Wook Park, Gyu-Jin Rho
ANTICANCER RESEARCH
(2011)