Article
Pharmacology & Pharmacy
Ali Salehi, Mohammad Amin Mobarhan, Javad Mohammadi, Hosein Shahsavarani, Mohammad Ali Shokrgozar, Atefeh Alipour
Summary: The decellularized onion scaffold with its proper 3D structure and supportive role on osteogenic differentiation showed promising potential for use in tissue engineering. This study demonstrated that the scaffold supported stem cell growth and proliferation, as well as enhanced osteogenic differentiation compared to traditional 2D culture systems.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xifeng Liu, Linli Li, Bipin Gaihre, Sungjo Park, Yong Li, Andre Terzic, Benjamin D. Elder, Lichun Lu
Summary: Functional spheroids engineered with two-dimensional heteronano-layers consisting of black phosphorus and graphene oxide show promising potential for bone tissue engineering. These spheroids successfully support osteogenesis of mesenchymal stem cells, neovascularization, and bone regeneration in vivo, as well as achieve outstanding in vivo osteogenic induction and bone regrowth without prior in vitro culture of osteogenic factor. The shortened overall culture time could be advantageous for clinical translation.
Article
Cell & Tissue Engineering
Hadi Sadeghzadeh, Ahmad Mehdipour, Hassan Dianat-Moghadam, Roya Salehi, Ali Baradar Khoshfetrat, Ayla Hassani, Daryush Mohammadnejad
Summary: The study fabricated a multilayered nanocomposite scaffold containing magnetic collagen to promote osteogenic differentiation in ADSCs without the need for osteogenic differentiation medium. The magnetic nanocomposite scaffold enhanced scaffold strength, wettability, porosity, and biocompatibility, as well as facilitated ALP activity and calcium mineralization.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Engineering, Biomedical
Yanqun Li, Jianhui Yue, Yuan Liu, Jun Wu, Min Guan, Di Chen, Haobo Pan, Xiaoli Zhao, William W. Lu
Summary: The study revealed that strontium promotes osteogenic differentiation of mesenchymal stem cells and balances stemness maintenance and osteogenic differentiation through asymmetric cell division. Strontium activates noncanonical Wnt signaling to regulate cell division and enhance osteogenic differentiation.
ACTA BIOMATERIALIA
(2021)
Article
Cell Biology
Dongdong Fei, Yanmin Xia, Qiming Zhai, Yazheng Wang, Feng Zhou, Wanmin Zhao, Xiaoning He, Qintao Wang, Yan Jin, Bei Li
Summary: This study focuses on the clonal osteogenic heterogeneity of periodontal ligament stem cells (PDLSCs) and uncovers a mechanism where exosome-mediated PINK1/Parkin-dependent mitophagy regulates interclonal communication among SCCs with osteogenic heterogeneity, promoting osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Shiva Shariati, Ehsan Seyedjafari, Fatemeh Sadat Mahdavi, Amirhosein Maali, Elaheh Ferdosi-Shahandashti
Summary: In this study, NiFe2O4/ZnO-coated PLLA scaffold was used to evaluate the osteogenic differentiation capability of hAMSCs. The results showed that the NiFe2O4/ZnO-coated PLLA scaffold could enhance the adhesion, proliferation, and differentiation of hAMSCs, as well as promote the expression of osteogenic markers.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Polymer Science
Jiyong Park, Sangbae Park, Jae Eun Kim, Kyoung-Je Jang, Hoon Seonwoo, Jong Hoon Chung
Summary: The study utilized graphene oxide (GO) as a coating material to enhance the osteogenic differentiation ability of poly(epsilon-caprolactone) (PCL) scaffolds, promoting cell proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs).
Article
Biochemistry & Molecular Biology
Paulina Kazimierczak, Malgorzata Koziol, Agata Przekora
Summary: The newly developed biomaterial showed high biocompatibility and influenced macrophage polarization and osteoblast differentiation, playing an immunomodulatory role. The study demonstrated a low risk of inflammatory response and induced macrophage polarization into the osteogenic phenotype M2, making it a promising bone scaffold for regenerative medicine applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Man Zhang, Qian Sun, Yiling Liu, Zhiqin Chu, Leixiao Yu, Yong Hou, Heemin Kang, Qiang Wei, Weifeng Zhao, Joachim P. Spatz, Changsheng Zhao, Elisabetta A. Cavalcanti-Adam
Summary: Tunable mechanical properties of hydrogels play a key role in regulating stem cell differentiation. By modulating cellular spatial sensing of integrin-adhesive ligands on low stiffness hydrogels, the mechanosensing and osteogenic differentiation of MSCs can be promoted to levels comparable with those observed on stiffer hydrogels. This approach opens up new possibilities for designing biomaterials and tissue scaffolds for regenerative therapeutics.
Article
Biochemistry & Molecular Biology
Ziysan Buse Yarali Cevik, Ozan Karaman, Nermin Topaloglu
Summary: Photobiomodulation (PBM) is a novel strategy for bone tissue regeneration that utilizes red and near-infrared light absorption by mitochondria to trigger bone tissue regeneration. In this study, we aimed to enhance the osteogenic differentiation capability of a co-culture of hBMSC and HUVECs on 3D SFMs through triple light treatment at 655 and 808 nm wavelengths. We found that PBM therapy was successful in inducing osteogenesis, with 1 J/cm2 at 655 nm and 5 J/cm2 at 808 nm being the most effective energy densities for osteogenic differentiation on SFMs.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Liang Wang, Lei Qi
Summary: Recent studies have highlighted the important role of H19 in regulating stem cell osteogenic differentiation. The majority of the research findings support the positive regulatory effect of H19 through the lncRNA-miRNA pathway. Further investigation is needed to fully understand the function and underlying mechanisms of H19 in osteogenic differentiation.
MOLECULAR MEDICINE
(2021)
Article
Engineering, Biomedical
Alexandra McMillan, Minh Khanh Nguyen, Cong Truc Huynh, Samantha M. Sarett, Peilin Ge, Melanie Chetverikova, Kien Nguyen, David Grosh, Craig L. Duvall, Eben Alsberg
Summary: This study introduces a new strategy for delivering RNA-nanocomplexes from photocrosslinked dextran microspheres to achieve tunable presentation of bioactive RNA. The system enables sustained gene silencing within stem cell aggregates and has the potential to drive stem cell differentiation towards specific lineages.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Betuel Celebi-Saltik, Sena Babadag, Elif Ballikaya, Suat Pat, Mustafa Ozguer Oteyaka
Summary: Additive manufacturing is being increasingly used in dentistry and orthopedics for creating customized implants. In this study, fused deposition modeling was used to produce 3D scaffolds made of polyurethane filament. These scaffolds were coated with boric acid using the thermionic vacuum arc technique. The coated scaffolds showed macro-pores with dimensions of approximately 0.16 mm2. The boric acid coating increased roughness and decreased wettability. Cell culture analysis confirmed the presence of boric acid on the scaffolds. The 3D printed PU scaffolds with boric acid coating showed enhanced cell viability and osteogenic differentiation of dental pulp stem cells. The expression of osteogenic markers was increased in the coated scaffold group compared to the control group.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Bin Ren, Oliver B. Betz, Daniel Seitz, Christian Thirion, Michael Salomon, Volkmar Jansson, Peter E. Mueller, Volker M. Betz
Summary: This study evaluated a novel growth factor delivery system, consisting of adenoviral BMP-2 transduced fascia tissue, for its osteogenic effects on hADSCs seeded on an innovative biomimetic spongiosa-like scaffold. The results showed that delivery of BMP-2 by overexpressing fascia provided a continuous and uniform level of BMP-2 over a period of 30 days, leading to a strong osteogenic response of hADSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Jianhua Zhang, Hande Eyisoylu, Xiao-Hua Qin, Marina Rubert, Ralph Mueller
Summary: In this study, a novel human mesenchymal stem cell (hMSC)-laden GO/alginate/gelatin composite bioink was developed for forming 3D bone mimicking scaffolds. The GO composite bioinks showed improved bioprintability, scaffold fidelity, cell proliferation, osteogenic differentiation, and ECM mineralization. The optimal GO group was 1GO, which demonstrated great potential for 3D bioprinting of bone tissue models and tissue engineering applications.
ACTA BIOMATERIALIA
(2021)
Review
Dentistry, Oral Surgery & Medicine
Fabian Langenbach, Christian Naujoks, Ralf Smeets, Karin Berr, Rita Depprich, Norbert Kuebler, Joerg Handschel
CLINICAL ORAL INVESTIGATIONS
(2013)
Article
Engineering, Biomedical
Fabian Langenbach, Christian Naujoks, Andrea Laser, Michael Kelz, Pia Kersten-Thiele, Karin Berr, Rita Depprich, Norbert Kuebler, Gesine Koegler, Joerg Handschel
JOURNAL OF BIOMATERIALS APPLICATIONS
(2012)
Article
Engineering, Biomedical
Christian Naujoks, Fabian Langenbach, Karin Berr, Rita Depprich, Norbert Kuebler, Ulrich Meyer, Joerg Handschel, Gesine Koegler
JOURNAL OF BIOMATERIALS APPLICATIONS
(2011)
Article
Biochemical Research Methods
Fabian Langenbach, Karin Berr, Christian Naujoks, Andrea Hassel, Michael Hentschel, Rita Depprich, Norbert R. Kubler, Ulrich Meyer, Hans-Peter Wiesmann, Gesine Koegler, Joerg Handschel
Article
Cell & Tissue Engineering
Lydia Lammers, Christian Naujoks, Karin Berr, Rita Depprich, Norbert Kuebler, Ulrich Meyer, Fabian Langenbach, Beate Luettenberg, Gesine Koegler, Hans-Peter Wiesmann, Joerg Handschel
STEM CELL RESEARCH
(2012)
Article
Cell & Tissue Engineering
Joerg Handschel, Christian Naujoks, Fabian Langenbach, Karin Berr, Rita A. Depprich, Michelle A. Ommerborn, Norbert R. Kuebler, Matthias Brinkmann, Gesine Koegler, Ulrich Meyer
TISSUE ENGINEERING PART A
(2010)
Review
Cell & Tissue Engineering
Fabian Langenbach, Joerg Handschel
STEM CELL RESEARCH & THERAPY
(2013)