Article
Biochemistry & Molecular Biology
Moema de Alencar Hausen, Ariana de Souza Moraes, Flavia Pedrini, Felipe Grabarz, Jose Angelo Camilli, Eliana Aparecida de Rezende Duek
Summary: This study introduces a natural biomaterial device that can improve the wound healing process by modulating macrophage behavior in vitro. The device, assembled with adipose-derived stem cells and biocompatible polymers, promotes the release of anti-inflammatory cytokines, indicating the prevalence of M2 macrophages.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Luminita Labusca, Dumitru-Daniel Herea, Anca Emanuela Minuti, Cristina Stavila, Camelia Danceanu, Petru Plamadeala, Horia Chiriac, Nicoleta Lupu
Summary: Iron oxide based magnetic nanoparticles (MNP) have versatile applications in biology and medicine. This study compared the chondrogenic conversion of ADSC-MNP and WJMSC under different conditions, finding that ADSC-MNP displayed superior proliferation and chondrogenic potential, with MF exposure further enhancing chondrogenesis in ADSC-MNP. Loading ADSC with MNP may be a promising approach for cartilage engineering in the future.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shun-Cheng Wu, Chih-Hsiang Chang, Ling-Hua Chang, Che-Wei Wu, Jhen-Wei Chen, Chung-Hwan Chen, Yi-Shan Lin, Je-Ken Chang, Mei-Ling Ho
Summary: The study found that simvastatin (SIM) can enhance ADSC-based chondrogenesis in the presence of hyaluronan (HA) microenvironment (HAM) while avoiding osteogenesis.
Article
Biochemistry & Molecular Biology
Siti Syahira Airuddin, Ahmad Sukari Halim, Wan Azman Wan Sulaiman, Ramlah Kadir, Nur Azida Mohd Nasir
Summary: Stem cells, particularly adipose-derived stem cells, have shown promising potential in disease treatment due to their differentiation abilities and secretion of growth factors. However, more research is needed to fully understand the safety and effectiveness of adipose-derived stem cells in medical treatment.
Article
Cell & Tissue Engineering
Stefano Negri, Yiyun Wang, Takashi Sono, Qizhi Qin, Ginny Ching-Yun Hsu, Masnsen Cherief, Jiajia Xu, Seungyong Lee, Robert J. Tower, Victoria Yu, Abhi Piplani, Carolyn A. Meyers, Kristen Broderick, Min Lee, Aaron W. James
Summary: Research has shown that anti-DKK1 neutralizing antibodies can enhance the osteogenic differentiation of human adipose-derived stem cells, aiding in bone repair, and demonstrating potential beneficial effects in a mouse model.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Jana C. Blum, Thilo L. Schenck, Alexandra Birt, Riccardo E. Giunta, Paul S. Wiggenhauser
Summary: In this study, the combination of cell-derived artificial extracellular matrix with 3D printed polycaprolactone scaffolds showed promising results in improving bioactivity and differentiation of human adipose derived stem cells. The ac/aoPCL scaffolds demonstrated good biocompatibility and enhanced chondrogenic and osteogenic differentiation potential.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Oncology
Maria Rosaria Ambrosio, Giusy Mosca, Teresa Migliaccio, Domenico Liguoro, Gisella Nele, Fabrizio Schonauer, Francesco D'Andrea, Federica Liotti, Nella Prevete, Rosa Marina Melillo, Carla Reale, Concetta Ambrosino, Claudia Miele, Francesco Beguinot, Vittoria D'Esposito, Pietro Formisano
Summary: This study demonstrates that high glucose levels enhance the aggressiveness of breast cancer cells and promote a pro-tumorigenic phenotype in mammary adipose tissue-derived mesenchymal stromal/stem cells (MAT-MSCs). The findings suggest that metabolic control may be important for reducing cancer outgrowth and preserving the functions of the adipose microenvironment.
Article
Biochemical Research Methods
Fereshteh Valipour, Farzaneh Valipour, Reza Rahbarghazi, Amir Mohammad Navali, Mohammad Reza Rashidi, Soodabeh Davaran
Summary: The study successfully created a new biodegradable PCL-P (HEMA-NIPAAm) thermosensitive hydrogel scaffold and demonstrated its effectiveness in promoting chondrogenic differentiation of adipose-derived stem cells when combined with PDGF-BB. The results showed that the S-2 group exhibited good physical properties and swelling rate, with the scaffold having the potential to gradually release PDGF-BB. Cell culture experiments with human adipose-derived stem cells on the scaffold surface confirmed that PDGF-BB could promote cell attachment and proliferation.
JOURNAL OF BIOLOGICAL ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Jing Zheng, Yan Xie, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Summary: This study investigated different microenvironmental conditions for the controlled proliferation and chondrogenic differentiation of human bone-marrow-derived mesenchymal stem cells (hMSCs). The results showed that collagen sponges promoted cell proliferation and chondrogenic differentiation. Combining collagen sponges with collagen hydrogels and induction factors had the highest promotive effect on chondrogenic differentiation. These findings provide useful information for stem cell chondrogenic differentiation and cartilage tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yikai Wang, Zibo Liu, Chuqiao Pan, Yi Zheng, Yahong Chen, Xiang Lian, Yu Jiang, Chuhsin Chen, Ke Xue, Yuanyuan Zhang, Peng Xu, Kai Liu
Summary: This study found that extracellular vesicles derived from chondrocytes can enhance the chondrogenesis of adipose-derived stem cells in vitro, and low-intensity ultrasound stimulation can increase the secretion of these vesicles. Additionally, ultrasound-driven healing enhances the quantity and quality of the extracellular vesicles by enriching cartilage-regeneration-associated miRNAs.
Article
Cell & Tissue Engineering
Naoki Ishiuchi, Ayumu Nakashima, Satoshi Maeda, Yoshie Miura, Kisho Miyasako, Kensuke Sasaki, Toshio Uchiki, Ayano Sasaki, Shogo Nagamatsu, Naoki Nakao, Masataka Nagao, Takao Masaki
Summary: Fibrosis is a common histological feature in chronic organ injury, leading to organ failure. Adipose-derived mesenchymal stem cells (ASCs) have been studied as a therapeutic tool to prevent organ failure. This study compared the therapeutic effects of ASCs from superficial and deep layers and found that both types of ASCs are equally effective and safe for treating organ injury.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Engineering, Biomedical
Thomas L. Jenkins, Paula A. Sarmiento Huertas, Kentaro Umemori, Farshid Guilak, Dianne Little
Summary: This study aimed to evaluate crosslinking methods to enhance the retention of tendon-derived matrix (TDM) on electrospun poly(e-caprolactone) (PCL) scaffolds and stimulate tendon-like matrix synthesis. The results showed that crosslinking TDM to electrospun scaffolds improves tendon-like matrix synthesis, providing a viable strategy for improving the retention of TDM on electrospun PCL scaffolds.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Biochemistry & Molecular Biology
Seita Osawa, Hisashi Kato, Yuki Maeda, Hisashi Takakura, Junetsu Ogasawara, Tetsuya Izawa
Summary: This study investigated the impact of exercise training and high-fat diet on the differentiation potential of adipose-derived stem cells (ADSCs) and found variations in amino acid accumulation under different conditions. The findings also revealed the influence of exercise training on amino acid metabolism pathways in ADSCs-derived adipocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Surgery
Rui Guo, Jincai Fan
Summary: This study found that auricular chondrogenic-derived extracellular vesicles (AC-EVs) have a positive effect on cell proliferation and migration of adipose-derived mesenchymal stem cells (ADSCs), and they can promote chondrogenic differentiation of ADSCs. Compared with the commonly used cartilage inducer TGF-beta 3, AC-EVs showed better performance in the deposition of cartilage-specific matrix. Therefore, AC-EVs may be a useful ingredient for cartilage tissue engineering and external ear reconstruction.
AESTHETIC PLASTIC SURGERY
(2023)
Article
Medicine, Research & Experimental
Francesca Toia, Elena Lo Presti, Anna Barbara Di Stefano, Marta Di Simone, Marco Trapani, Anna Maria Corsale, Carmela Picone, Francesco Moschella, Francesco Dieli, Adriana Cordova, Serena Meraviglia
Summary: This study analysed the immunomodulatory properties of spheroids from adipose stem cells (SASCs) and compared them to adipose stem cells (ADSCs). SASCs showed stronger immunomodulatory abilities in heterologous experimental settings compared to ADSCs. SASCs also demonstrated greater anti-inflammatory properties.
Article
Engineering, Environmental
Najmeh Alsadat Abtahi, Seyed Morteza Naghib, Sina Jafari Ghalekohneh, Zahra Mohammadpour, Hojjatollah Nazari, Seyed Mohammad Mosavi, Seyed Mohammad Gheibihayat, Fateme Haghiralsadat, Javad Zavar Reza, Behroz Zandieh Doulabi
Summary: This study optimized niosomes for carrying and delivering anticancer agents and genes, showing that co-delivery of curcumin and miR-34a using the new cationic niosomal delivery system can be recognized as a prominent strategy for more effective cancer treatments. Cancer cells treated with co-delivery showed higher cytotoxicity, uptake, and anticancer activity, while showing less toxicity to normal human cells. In addition, the co-delivery of curcumin and miR-34a in the 4T1 xenografted Balb/C mouse tumor model resulted in a higher tumor inhibition rate compared to other groups.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Pooria Lesani, Gurvinder Singh, Zufu Lu, Mohammad Mirkhalaf, Elizabeth J. New, Hala Zreiqat
Summary: We report the design and development of a two-photon ratiometric carbon dot-based nanoprobe for real-time monitoring of intracellular pH. The nanoprobe exhibits sensitivity across a wide pH range, remarkable thermal and photo stability, as well as two-photon near-infrared excitation capability.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Materials Science, Biomaterials
Iman Roohani, Young Jung No, Betty Zuo, Sue D. Xiang, Zufu Lu, Hongwei Liu, Magdalena Plebanski, Hala Zreiqat
Summary: This study introduces a method to synthesize hollow submicron particles of beta-Tricalcium phosphate (β-TCP) at low temperatures and evaluates their cytocompatibility with human osteoblasts, adipose-derived stem cells, and antigen-presenting cells. The results demonstrate that h β-TCP significantly enhances osteogenic differentiation of stem cells, providing opportunities for developing new β-TCP-based biomaterials.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Dentistry, Oral Surgery & Medicine
Aisha Hussein Al-Jamaei, Jan G. A. M. de Visscher, V. Ramadugula Subramanyam, Tymour Forouzanfar, Peter Sminia, Behrouz Zandieh Doulabi, Marco N. Helder
Summary: This study investigated the effectiveness of WEE1 kinase inhibitor (MK-1775) in enhancing radiation responses of OTSCC cells in vitro. The results showed that MK-1775 inhibited the survival of OTSCC cells and enhanced their response to radiation by inducing apoptosis and lethal mitosis. This demonstrates the potential of WEE1 inhibitor as a potent radiosensitizer in OTSCC.
Article
Materials Science, Biomaterials
ZuFu Lu, Gurvinder Singh, Pooria Lesani, Hala Zreiqat
Summary: This article discusses the impact of cellular senescence on tissue regeneration capacity and explores the potential of nanotechnology in restoring and enhancing tissue regeneration ability in the aging population.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Saeid Afereydoon, Fateme Haghiralsadat, Nima Hamzian, Ali Shams, Mahdie Hemati, Seyed Morteza Naghib, Masoud Shabani, Behrouz Zandieh-doulabi, Davood Tofighi
Summary: This study demonstrates that pre-treatment with curcumin-containing nanoniosomes as a radiosensitizer enhances irradiation-induced breast cancer cell apoptosis, making it an effective strategy to increase the effectiveness of breast cancer therapy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Oncology
Aisha A. H. Al-Jamaei, Jan G. A. M. de Visscher, Tymour Forouzanfar, Ruud H. Brakenhoff, C. Rene Leemans, Arwen Stikvoort, Behrouz Zandieh-Doulabi, Marco N. Helder
Summary: This study reveals that irradiation leads to upregulation of c-Met expression in oral tongue squamous cell carcinoma (OTSCC) cells, and enhances cell migration and invasiveness. These findings provide new insights into the role and dynamics of c-Met in OTSCC.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Geriatrics & Gerontology
ZuFu Lu, Liting Jiang, Pooria Lesani, WenJie Zhang, Ning Li, Danyang Luo, Yusi Li, Yulin Ye, Ji Bian, Guocheng Wang, Colin R. Dunstan, XinQuan Jiang, Hala Zreiqat
Summary: This study shows that nicotinamide mononucleotide (NMN) could partially reverse the negative effects of tumor necrosis factor-alpha (TNF-α) on osteoblasts, including inducing senescence, reducing differentiation ability, and affecting mitochondrial function. In addition, NMN can protect cells from aging damage by increasing mitophagy activity.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Article
Cell & Tissue Engineering
Hadi Seddiqi, Sonia Abbasi-Ravasjani, Alireza Saatchi, Ghassem Amoabediny, Behrouz Zandieh-Doulabi, Jianfeng Jin, Jenneke Klein-Nulend
Summary: This study investigates the effects of bioreactor systems on bone tissue engineering using cell-seeded 3D-printed scaffolds. The results indicate that perfusion bioreactors enhance osteogenic activity more strongly than spinner flask bioreactors.
TISSUE ENGINEERING PART C-METHODS
(2023)
Article
Materials Science, Biomaterials
Zhengjiang Xu, Yuan Zhang, Danping Lu, Guofang Zhang, Yang Li, Zufu Lu, Fei Wang, Guocheng Wang
Summary: Accumulation of senescent cells in the elderly has a negative impact on bone homeostasis, and it is important to mitigate cell senescence and reduce oxidative stress for improved bone fracture healing in elderly patients. This study developed a nanoformulation of resveratrol (RSV) using a biocompatible zeolitic imidazolate framework-8 (ZIF-8) nanoparticle, which effectively addressed the issues of oxidation and bioavailability. The ZIF-8/RSV nanoformulation showed promising results in alleviating cellular senescence and enhancing cell functions, suggesting its potential for bone fracture treatment in the elderly.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Engineering, Biomedical
Charenpreet Virdi, Zufu Lu, Hala Zreiqat, Young Jung No
Summary: This study demonstrates that high-water content polyvinyl alcohol hydrogels can achieve a tensile strength exceeding 1.0 MPa through a combination of physical crosslinking, mechanical drawing, freeze drying, and hierarchical design.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Chunfeng Xu, Mingjie Wang, Behrouz Zandieh Doulabi, Yuanyuan Sun, Yuelian Liu
Summary: Osteosarcoma (OS) is a rare and aggressive tumor that is typically treated with extended surgical resections, resulting in bone defects and decreased post-surgical quality of life. An ideal therapeutic agent for OS should have both anti-cancer and bone repair capabilities. Curcumin (CUR) has been studied for its potential in OS therapy and bone regeneration; however, its mechanism of action in suppressing OS development is not fully understood. Despite its role as a natural antioxidant, this research found that CUR actually increased intracellular reactive oxygen species (ROS) levels, damaged DNA, and induced apoptosis in MG-63 cells in vitro, even though it also increased levels of NRF2 protein and NRF2-regulated antioxidase genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Victor J. B. van Santen, Behrouz Zandieh Doulabi, Cornelis M. Semeins, Jolanda M. A. Hogervorst, Cornelia Bratengeier, Astrid D. Bakker
Summary: Once prostate cancer cells metastasize to bone, they experience 2 kPa compression, which stimulates the epithelial-to-mesenchymal transition (EMT) and alters their secretion of paracrine signals. This leads to a decrease in osteoclast resorptive activity and an increase in osteoblast mineralization. These findings provide insight into the altered behavior of prostate cancer cells under mechanical stimuli and suggest potential leads for therapies to prevent bone metastases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Fatemeh Araste, Astrid Diana Bakker, Behrouz Zandieh-Doulabi
Summary: The use of nanoparticles in antiviral strategies, such as vaccine development and functionalization of face masks, shows promising potential. However, there are concerns about their potential side effects and environmental impacts, which need to be addressed to ensure safe and effective usage.
JOURNAL OF NANOPARTICLE RESEARCH
(2023)
Article
Cell Biology
Fardin Rahimi, Ghasem Amoabediny, Hossein Sabahi, Behrouz Zandieh-Doulabi
Summary: This study aimed to develop lipo-niosomes loaded with Amphotericin B (AmB) and Thymus Essential Oil (TEO) to treat fungal-infected human adipose stem cells (hASCs). The optimal formulation of the lipo-niosomes was characterized and their effectiveness was evaluated. Results showed that the lipo-niosomes had high encapsulation efficiency, controlled drug release, and low toxicity. The antifungal activity of the lipo-niosomes was significantly higher than that of free formulations. This suggests that the AmB/TEO-loaded lipo-niosomes could be a promising nano-system for treating fungal infections in hASCs.