Article
Biochemistry & Molecular Biology
Megumi Miyabe, Nobuhisa Nakamura, Tomokazu Saiki, Satoru Miyabe, Mizuho Ito, Sachiko Sasajima, Tomomi Minato, Tatsuaki Matsubara, Keiko Naruse
Summary: Atherosclerosis is a leading cause of death globally, and there is a correlation between periodontal disease and atherosclerosis. This study used Porphyromonas gingivalis-derived LPS (Pg-LPS) to investigate the proliferation and migration of human aortic smooth muscle cells (HASMCs). The results suggest that Pg-LPS promotes atherosclerosis through the activation of the TLR4-MAPK signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Hsiao-Ya Tsai, Jen-Chun Wang, Yu-Juei Hsu, Yi-Lin Chiu, Chih-Yuan Lin, Cheng-Yo Lu, Shih-Hung Tsai
Summary: This study evaluated the roles of RUNX2 and miR-424/322 in the progression of AAA and found that upregulation of RUNX2 and miR-424 is associated with AAA. Analysis showed that upregulation of RUNX2 can induce AAA progression by promoting MMPs expression, dependent on Smad2/3 upregulation. Additionally, miR-424 is also involved in AAA development through downregulation of RUNX2. Results demonstrated that siRUNX2 and miR-424 mimics can attenuate the overexpression of AAA progression-related molecules. miR-322 KO mice were susceptible to AngII-induced AAA, while silencing of RUNX2 and administration of exogenous miR-322 mimics improved AAA progression. Overall, this study revealed the significant role of the Smad/RUNX2/miR-424/322 axis in AAA pathogenesis and demonstrated the potential therapeutic value of miR-424/322 mimics and RUNX2 inhibitor for AAA progression.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Cardiac & Cardiovascular Systems
Hui Zheng, Zhihuang Qiu, Tianci Chai, Jian He, Yuling Zhang, Chaoyun Wang, Jianqiang Ye, Xiaohui Wu, Yumei Li, Li Zhang, Liangwan Chen
Summary: The study found an association between insulin resistance (IR) and aortic dissection (AD). Insulin resistance promotes the occurrence of AD by inducing phenotypic switching of vascular smooth muscle cells. This provides a basis for further research on the mechanism of how IR results in AD and a new approach for the prevention and treatment of AD.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Anne Virsolvy, Aurelie Fort, Lucie Erceau, Azzouz Charrabi, Maurice Hayot, Franck Aimond, Sylvain Richard
Summary: The study revealed the important role of Na-v channels in vasomotion of human arteries, with activation of these channels leading to rhythmic oscillatory contractile activity in the vasculature.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Erik Schoenmakers, Federica Marelli, Helle F. Jorgensen, W. Edward Visser, Carla Moran, Stefan Groeneweg, Carolina Avalos, Sean J. Jurgens, Nichola Figg, Alison Finigan, Neha Wali, Maura Agostini, Hannah Wardle-Jones, Greta Lyons, Rosemary Rusk, Deepa Gopalan, Philip Twiss, Jacob J. Visser, Martin Goddard, Samer A. M. Nashef, Robin Heijmen, Paul Clift, Sanjay Sinha, James P. Pirruccello, Patrick T. Ellinor, Elisabeth M. Busch-Nentwich, Ramiro Ramirez-Solis, Michael P. Murphy, Luca Persani, Martin Bennett, Krishna Chatterjee
Summary: This article reports a heritable deficiency disorder of selenoproteins caused by mutations in SECISBP2, which leads to oxidative stress-mediated aortic cell death, predisposing to thoracic aortic aneurysm formation.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Jian Dong, Shuangshuang Li, Zilin Lu, Pengcheng Du, Guangqin Liu, Mintao Li, Chao Ma, Jian Zhou, Junmin Bao
Summary: HCMV-miR-US33-5p suppresses proliferation and key factors of the integrin signal pathway, and also inhibits the expression of EPAS1/SLC3A2, leading to increased HA-VSMC apoptosis. These findings provide new insights into therapeutic strategies for AAD.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2022)
Article
Pharmacology & Pharmacy
Do Young Kim, Jiyuan Piao, Jeong Seop Park, Dahyeon Lee, Hyun Sook Hong
Summary: The study found that SP can mitigate damage to endothelial cells caused by TNF-alpha and protect the endothelium by maintaining low ECM levels. This protective effect is mediated by Akt activation and NO-enriched conditions, and it also reduces MCP-1 production in aortic smooth muscle cells. The results suggest that SP may prevent cardiovascular disease by protecting aortic endothelial and smooth muscle cells from inflammatory injury.
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Zachary Ladd, Gang Su, Joseph Hartman, Guanyi Lu, Sara Hensley, Gilbert R. Upchurch Jr, Ashish K. Sharma
Summary: This study explored the regulation role of spironolactone in endothelial cell-mediated ATP release for the mitigation of abdominal aortic aneurysm (AAA) formation. The results showed that spironolactone treatment could decrease the diameter of the abdominal aorta, reduce elastic fiber disruption, and immune cell infiltration. Furthermore, it also suppressed the expression of pro-inflammatory cytokines, inhibited MMP2 activity, and reduced ATP content in the aortic tissue. In vitro experiments demonstrated that spironolactone attenuates extracellular ATP release from endothelial cells, leading to decreased macrophage activation and smooth muscle cell-dependent vascular remodeling.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Anmol Kumar, Stefan Mutter, Erika B. Parente, Valma Harjutsalo, Raija Lithovius, Sinnakaruppan Mathavan, Markku Lehto, Timo P. Hiltunen, Kimmo K. Kontula, Per-Henrik Groop
Summary: This study investigated the effect of L-type calcium channel blockers (LTCCBs) on VEGF concentrations in retinal cells and human serum, and evaluated the association between LTCCB usage and risk of severe diabetic eye disease (SDED). The results showed that LTCCBs increase VEGF concentrations in retinal cells and human serum, but they are not associated with the risk of SDED in adults with type 1 diabetes.
Article
Biochemistry & Molecular Biology
Shuo Wang, Xiaoxiang Tian, Dan Liu, Xiaolin Zhang, Chenghui Yan, Yaling Han
Summary: This study found that overexpression of TRPV5 could decrease the incidence and diameter of abdominal aortic aneurysm, inhibit collagen and elastin degradation, increase alpha-SMA, and reduce MMP2 expression. TRPV5 also played a role in regulating VSMC phenotype switch by downregulating KLF4 expression, suggesting it as a potential new strategy for treating AAA.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Cell Biology
Sou Inagaki, Yoshiaki Suzuki, Keisuke Kawasaki, Rubii Kondo, Yuji Imaizumi, Hisao Yamamura
Summary: In this study, the role of mitochondrial fusion protein 2 (Mfn2) in the coupling of sarcoplasmic reticulum (SR) and mitochondria in vascular smooth muscle cells (VSMCs) was examined. It was found that Mfn2 is responsible for tethering mitochondria to SR, which regulates calcium signaling, ATP production, and cell proliferation in VSMCs.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Engineering, Biomedical
Mi Hu, Fan Jia, Wei-Pin Huang, Xu Li, Deng-Feng Hu, Jing Wang, Ke-Feng Ren, Guo-Sheng Fu, Yun-Bing Wang, Jian Ji
Summary: The stiffness of the vascular extracellular matrix plays a crucial role in artery pathology and can impact the autophagy levels of vascular endothelial cells and smooth muscle cells. Increasing substrate stiffness leads to differential changes in autophagy levels, affecting gene expression associated with endothelial function and transitioning VSMCs from contractile to synthetic phenotype. Inhibiting cell autophagy further reduces endothelial functional gene expression and increases VSMC calponin expression, highlighting the important role of autophagy in response to changes in microenvironment stiffness.
BIOACTIVE MATERIALS
(2021)
Article
Urology & Nephrology
Ying Gong, Qing Zhong, Yunfeng Xia, Yang Wen, Hua Gan
Summary: This study investigated the expression and role of a lncRNA, MALAT1, and a miRNA, miR-30c, in vascular calcification (VC). The results showed that MALAT1 promoted VSMCs calcification, at least partially through regulating the miR-30c/Runx2 axis.
Article
Microbiology
Koon-Chu Yaiw, Abdul-Aleem Mohammad, Chato Taher, Huanhuan Leah Cui, Helena Costa, Ourania N. Kostopoulou, Masany Jung, Alice Assinger, Vanessa Wilhelmi, Jiangning Yang, Klas Straat, Afsar Rahbar, John Pernow, Cecilia Soderberg-Naucler
Summary: The study found that HCMV infection significantly decreased ET-1 expression, promoted accumulation of ET-1 precursor protein in infected endothelial cells, and inhibited ET-1 maturation. Treatment with Ganciclovir did not prevent the suppressive effects of the virus on ET-1 expression. The IE2-p86 protein was identified to predominantly modulate ET-1 expression.
Article
Biology
Guoqing Qian, Oluwaseun Adeyanju, Ayobami Olajuyin, Xia Guo
Summary: This article provides a summary of the risk factors, pathophysiology, and established animal models of abdominal aortic aneurysm (AAA). It focuses specifically on the vascular smooth muscle cell phenotypic switching and dysfunction in AAA formation, offering potential novel therapeutic targets for the treatment or prevention of the disease.
Article
Engineering, Biomedical
Divya B. Patel, Christopher R. Luthers, Max J. Lerman, John P. Fisher, Steven M. Jay
ACTA BIOMATERIALIA
(2019)
Article
Cell & Tissue Engineering
Bai Yang, Cecillia Lui, Enoch Yeung, Hiroshi Matsushita, Anjana Jeyaram, Isaree Pitaktong, Takahiro Inoue, Zayneb Mohamed, Chin Siang Ong, Deborah DiSilvestre, Steven M. Jay, Leslie Tung, Gordon Tomaselli, Chunye Ma, Narutoshi Hibino
TISSUE ENGINEERING PART C-METHODS
(2019)
Review
Biochemical Research Methods
Alex Eli Pottash, Christopher Kuffner, Madeleine Noonan-Shueh, Steven M. Jay
JOURNAL OF BIOLOGICAL ENGINEERING
(2019)
Article
Pharmacology & Pharmacy
John S. Schardt, Madeleine Noonan-Shueh, Jinan M. Oubaid, Alex Eli Pottash, Sonya C. Williams, Arif Hussain, Rena G. Lapidus, Stanley Lipkowitz, Steven M. Jay
Article
Cell & Tissue Engineering
Enoch Yeung, Takuma Fukunishi, Yang Bai, Djahida Bedja, Isaree Pitaktong, Gunnar Mattson, Anjana Jeyaram, Cecillia Lui, Chin Siang Ong, Takahiro Inoue, Hiroshi Matsushita, Sara Abdollahi, Steven M. Jay, Narutoshi Hibino
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2019)
Article
Engineering, Biomedical
Charlotte Piard, Anjana Jeyaram, Yi Liu, John Caccamese, Steven M. Jay, Yu Chen, John Fisher
Article
Biotechnology & Applied Microbiology
Anjana Jeyaram, Tek N. Lamichhane, Sheng Wang, Lin Zou, Eshan Dahal, Stephanie M. Kronstadt, Daniel Levy, Babita Parajuli, Daphne R. Knudsen, Wei Chao, Steven M. Jay
Review
Biotechnology & Applied Microbiology
Louis J. Born, John W. Harmon, Steven M. Jay
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2020)
Editorial Material
Biotechnology & Applied Microbiology
Steven M. Jay
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2020)
Article
Biochemical Research Methods
Pricila Hauk, Kristina Stephens, Chelsea Virgile, Eric VanArsdale, Alex Eli Pottash, John S. Schardt, Steven M. Jay, Herman O. Sintim, William E. Bentley
ACS SYNTHETIC BIOLOGY
(2020)
Review
Neurosciences
Dipankar Dutta, Niaz Khan, Junfang Wu, Steven M. Jay
Summary: Extracellular vesicles (EVs) are membrane-delimited particles secreted by nearly all cell types, playing crucial roles in mediating physiological functions and pathophysiological processes in the central nervous system. Research has shown that EVs are important in neurotrauma such as spinal cord injury (SCI) and may serve as potential targets for therapy.
TRENDS IN NEUROSCIENCES
(2021)
Article
Engineering, Biomedical
Louis J. Born, Kai-Hua Chang, Pouria Shoureshi, Frank Lay, Sameer Bengali, Angela Ting Wei Hsu, Sanaz Nourmohammadi Abadchi, John W. Harmon, Steven M. Jay
Summary: This study explores the potential of using extracellular vesicles (EVs) for delivery of HOTAIR as a strategy to promote healing of chronic wounds.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Multidisciplinary Sciences
Adela S. Oliva Chavez, Xiaowei Wang, Liron Marnin, Nathan K. Archer, Holly L. Hammond, Erin E. McClure Carroll, Dana K. Shaw, Brenden G. Tully, Amanda D. Buskirk, Shelby L. Ford, L. Rainer Butler, Preeti Shahi, Kateryna Morozova, Cristina C. Clement, Lauren Lawres, Anya J. O' Neal, Choukri Ben Mamoun, Kathleen L. Mason, Brandi E. Hobbs, Glen A. Scoles, Eileen M. Barry, Daniel E. Sonenshine, Utpal Pal, Jesus G. Valenzuela, Marcelo B. Sztein, Marcela F. Pasetti, Michael L. Levin, Michail Kotsyfakis, Steven M. Jay, Jason F. Huntley, Lloyd S. Miller, Laura Santambrogio, Joao H. F. Pedra
Summary: Extracellular vesicles play a role in the transmission of pathogens from arthropods to hosts, with different types of tick-derived vesicles promoting or mitigating bacterial infection outcomes by influencing skin immunity.
NATURE COMMUNICATIONS
(2021)
Review
Materials Science, Biomaterials
Nicholas H. Pirolli, William E. Bentley, Steven M. Jay
Summary: Bacterial extracellular vesicles (BEVs) have been identified as potential signaling vectors for long-distance interkingdom communication within the gut-microbiota brain axis, with the ability to regulate brain gene expression and induce pathology in neuroinflammatory and neurodegenerative conditions. BEVs also have therapeutic properties that can be utilized in probiotic therapy, drug delivery, and vaccine development.
Review
Pharmacology & Pharmacy
Stephanie M. Kronstadt, Alex E. Pottash, Daniel Levy, Sheng Wang, Wei Chao, Steven M. Jay
Summary: EVs, as nanosized cell-derived particles, play critical roles in regulating sepsis pathophysiology and have demonstrated anti-inflammatory, anti-apoptotic, and immunomodulatory effects in sepsis models. Future sepsis therapies may involve using EVs to enhance and control therapeutic effects.
ADVANCED THERAPEUTICS
(2021)